LOCUS       NZ_CP101215             4236 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-147 plasmid pEC21Z147-4K, complete
            sequence.
ACCESSION   NZ_CP101215
VERSION     NZ_CP101215.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593414
            Assembly: GCF_024298765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 177 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 66 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101215.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-147"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z147-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            complement(110..2329)
                     /gene="mobQ"
                     /locus_tag="NML26_RS27245"
                     /old_locus_tag="NML26_27250"
     CDS             complement(110..2329)
                     /gene="mobQ"
                     /locus_tag="NML26_RS27245"
                     /old_locus_tag="NML26_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_112917959.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPVRRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCEGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM"
                     /name="mobQ"
     gene            2508..2783
                     /locus_tag="NML26_RS27250"
                     /old_locus_tag="NML26_27255"
     CDS             2508..2783
                     /locus_tag="NML26_RS27250"
                     /old_locus_tag="NML26_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052995416.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNG"
                     /name="hypothetical protein"
     gene            complement(2989..3897)
                     /locus_tag="NML26_RS27255"
                     /old_locus_tag="NML26_27260"
     CDS             complement(2989..3897)
                     /locus_tag="NML26_RS27255"
                     /old_locus_tag="NML26_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072656801.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGLAKGEAYEDKRFMALCMLENGYSQ
                     KAIAAMLDVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     Typing          1972..2330
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2331..2501
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="97.0"
                     /evalue="1.23e-74"
     Typing          complement(3170..3897)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.982"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(114..2329)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.963"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgaacgggtc aaaacggcaa actttctcat ccagaaaacg cctcacggga cgcggaattt
       61 tttcgtgtcc cgtgagacgt tggcggggtt cggggtgcag ccccggatat cacatactca
      121 tgccccggta atgtccgggg cgtcgttgtt gttgcctgtg ctgttcgtcc cgcttccatt
      181 catgccaggt ctgattaaat ccgagttgac ggaagagata atccggcatc tcttccggtt
      241 tgttgatggc agcaaaaagc gtgtcctgac agcccttaag cctgaactgc atcagcttcg
      301 gttccggtgg gtacggagta tcagcatcac ggtttatggc ctcaccgtaa cctgccaggt
      361 gtatcccgtt ctctgtgatg atgcttacca tcagatgact gtaatccggc gacggaagcg
      421 caagaacggg acgcagctcg tcctccaggc gcacaaaacc gacaagcatt tcctccggcc
      481 cttcgcacaa cagcagattc agcgtcctgc tgtttcgctc tgtcgaaaag acaacgggca
      541 tgagttgctc cggtgccgca taagcggtga cgtccacccg gtgcgctgac ggcagcggag
      601 aggctgcggg cctgcggtcg tcgctgaaat aaaaattatc cggccagtga tgcggcagcg
      661 ccggaaaacc gtaagagggc cacccctggc gcatctgttc cagtacatgc cggaccgtcg
      721 caccggaata cattctcaca cgctcaggcc gcgccatcgt ctcatgagat tctggcattc
      781 cctcacgtat atccgagacc atcacagccc cgcctggcga caggtaaaca ttcaccgggt
      841 catgaacact ctgggggtaa tgcgccagct catcacccca taccgtgaat tctccggctc
      901 tgttactcag catcatgtag cgggttatcc tgcccgttcc cggctctctg gcacgcccca
      961 gctccgtaat aaccccggct aatgccgttt ctcggctcag gcgatggtta tcatggtgtt
     1021 tcagccgtgc gtcatattcc tgttccttcc ggcgcgcttc cgcttcggct tcctccctgc
     1081 gccgctccgc ttcccgtgct gacagttttt ctttcagctc tgccattccc ggcgaaatcc
     1141 acgtattcca ctgcgtttca aaggcctgac gacggacggg aatgtcccgt tctttcacgg
     1201 ccctgtaggt gcggagaaga gattcaatct gtttctcgag tttccagaag cggagttgtc
     1261 ttttgcggta ttccggttca gcccatgtca tcaggggaag ctctgacagg atggacgcta
     1321 ccggacgggc ttttctgagt ccggtcatct ctttttcgag aatatcccgc tctttcacca
     1381 gcgcattccc ctcacgtttg atgtcgtttg ccttcccgtt catcgccacg aactccggtg
     1441 tcatctccca tgcgttcccg cattcctgcg gcagtgtggc aatgaattcc cgcagggtat
     1501 ccgctgaggc attcgcggcg ggaatggtca gattaagggc ttccgctgca cgacgtgcgg
     1561 catcgctgag ttcccgtttc cgcacgttct cctgtacctg gatgactgcc agccttgtgc
     1621 gggaaacctg tatttccagg ttaaggctgt taatcaggcg gttctgctca ccgcgttctg
     1681 tttccatgcc ccgtttttcc atcgcacagg cagcctttcc gaggtgaagg cccggcgctt
     1741 tctccagtcc ctgacgctcg tatgaacgat ggtctatctc ttcctggtag ccggcgttcg
     1801 caagcgctct gttcgcatgg tcagcccatg atgcgcgcca cgtctcggcg agtgcccggt
     1861 cgttccagtc cctgaccttt cctgcgaatc ccgtttcccc gacagctctc gtggtcagca
     1921 tgatgtgtgc atgggggttg gtccggtcca tatgatggaa cgccacatcg gcaatcatgc
     1981 cccgactgac aaagttttcc cggacaaaag cgagaacggc ctcccgtgcg gcctcgcggg
     2041 aaatctcacg gggaatggcg agttcaatct cccttgccag ctgggagttc cggcgctgtt
     2101 cggctttctc gaccgcattc cagagaacgg aacggtcacc acaccaggac ggtgcattcg
     2161 caggggtgag aatgaccgca tcatcaacgc cgcgtttacg ggtgtaatcg tggattaatc
     2221 cggtacggtc atcgggtatg acagagccgg aacggtaggc agcagaagca acggcactgt
     2281 agccgttttt tcgcgaaatg attttcatgc tgagatgata aatcgccatt tctgccgtaa
     2341 tgcctttttg ccgttttctt tcagccccgc agggcgcaca caatcgaaac gaagtgaaga
     2401 ttgtataagt gcgcacttcg tgatatgccg tgcattatgc cagctcagtt tttaaactac
     2461 cagtgacgag ctaaaatgcg atttttcacc gcgcaaggag tcacgaaatg agcagaacac
     2521 tcgaacagaa gattgctgaa gctgaggcca gattgcagcg cctgaaggcg aagagccgga
     2581 gcctggacac tgcgcaaaaa gtcattgtgg gagcagccat gctggcgagg gtacggaggc
     2641 cggaagaggc gcaactgcgt gcgttcctgc tccagttcct gcgaaaggaa gtcacaagac
     2701 aggccgatgt gaaccggcta caaccactga ttaacgagct ggagaagcta ccaagaccac
     2761 ctgcgaaacc gcagaacgga taagccccgg cgcgtaagtg ccacatgcac caccggacgc
     2821 ctttgcgctt cctcgcacat tcgctcgcta cgctcggtca tgaggctgcg gcgagcgcta
     2881 ccaccgcaat cacgaggaaa ccagcgccat tgctgggcgg aaccggaaaa accgcaggaa
     2941 cgccaacagc tcaaaaaagc gccgaaaagg cgctgttatc tgataaggct atttgtgcat
     3001 tgcccaatta cggatcgtat ttcgatgtac atccagcatc gccgcaatag ctttctgaga
     3061 atatccattc tccagcatac atagcgccat gaaacgcttg tcctcgtagg cttcgccttt
     3121 agcaaggcca cctttccggc ctcgagcggc ctgtacagcg gaaaagcctt ccggtgagaa
     3181 ttttctgtgc gtgtatttcg caatgctctt gccaatagcc cggcattcag ccagtgaaag
     3241 aggaacggga agcgatgcgt tgtacatttc gacacgctgg atcacggcat caagccactg
     3301 tgagaatgct ggccatccct gacgaatcgc tctgtaagcc catttgcgcg tcatttcgaa
     3361 caggtggcag tttcgcccca tcccgtaatg tttatcgatg ctacgacgct ctgaggcgct
     3421 caaatcgaga taatctgcca gttcatcgag ggtataggct tcctcgcgcc actccatcac
     3481 ctgccattcc aggtggaacg gatttttgca aatcagtccg ctgtagttca catcagcaca
     3541 aagtttctca cacagcgaac gttcaatcgc ggcagcatat ttcagcgcct taaccgacgc
     3601 atcaggcgcg gttctcacgg cgatattgag tgcatagagc aggtgagcgt gtccgttcac
     3661 aggattttta acggtgatgt tcggcgcggg ggcgttccgg tcgctccagt caatcgctgc
     3721 cccctcgcgg tcaacgtcaa aaaccagcca gaactgcgca tgaggctggt tctgctggat
     3781 gtaccgcgca agaagcgcac gccctttgcc ggagatgcga aggccgaaag cgagatcatc
     3841 cgtgtgatag ggacggtggg gtaaattttc ttcgaagtat tgaagcgcgg cgctcatgtg
     3901 ttacgattcc ttccaggtta tcgaccgaca tcgcccctgt attttcgtga cagtcttgcc
     3961 ggataggcac gaatttcagg ggcgtttgtt ttttctggcg cttaccctac acccaaacgc
     4021 caaaccctgt cgatccttcc ccaaaacccg atccatttac cataactccc gttgtacgta
     4081 ccctctaaaa cgctctcaga cgcgttctga cgcaaaaaac gcaaaaagac aaggaaacac
     4141 caggctgaac cagaaaaatg cgtcagaatc gtttttaggg cgctgcataa ctatgcacga
     4201 aagcgatgcg cgatcggttt cgaccgttca gaagcg
//
