LOCUS       NZ_CP101214             6988 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-147 plasmid pEC21Z147-7K, complete
            sequence.
ACCESSION   NZ_CP101214
VERSION     NZ_CP101214.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593414
            Assembly: GCF_024298765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 177 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 66 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101214.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-147"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z147-7K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            join(6456..6988,1..28)
                     /locus_tag="NML26_RS27185"
                     /old_locus_tag="NML26_27190"
     CDS             join(6456..6988,1..28)
                     /locus_tag="NML26_RS27185"
                     /old_locus_tag="NML26_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            35..268
                     /locus_tag="NML26_RS27190"
                     /old_locus_tag="NML26_27195"
     CDS             35..268
                     /locus_tag="NML26_RS27190"
                     /old_locus_tag="NML26_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(752..1174)
                     /locus_tag="NML26_RS27195"
                     /old_locus_tag="NML26_27200"
     CDS             complement(752..1174)
                     /locus_tag="NML26_RS27195"
                     /old_locus_tag="NML26_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_048236912.1"
                     /translation="MKSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1296..1574
                     /locus_tag="NML26_RS27200"
                     /old_locus_tag="NML26_27205"
     CDS             1296..1574
                     /locus_tag="NML26_RS27200"
                     /old_locus_tag="NML26_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1566..1856)
                     /locus_tag="NML26_RS27205"
                     /old_locus_tag="NML26_27210"
     CDS             complement(1566..1856)
                     /locus_tag="NML26_RS27205"
                     /old_locus_tag="NML26_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            2096..3742
                     /gene="cka"
                     /locus_tag="NML26_RS27210"
                     /old_locus_tag="NML26_27215"
     CDS             2096..3742
                     /gene="cka"
                     /locus_tag="NML26_RS27210"
                     /old_locus_tag="NML26_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_162754150.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKVRENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(3739..4029)
                     /gene="cki"
                     /locus_tag="NML26_RS27215"
                     /old_locus_tag="NML26_27220"
     CDS             complement(3739..4029)
                     /gene="cki"
                     /locus_tag="NML26_RS27215"
                     /old_locus_tag="NML26_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_113402713.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNENDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            4059..4190
                     /locus_tag="NML26_RS27220"
                     /old_locus_tag="NML26_27225"
     CDS             4059..4190
                     /locus_tag="NML26_RS27220"
                     /old_locus_tag="NML26_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4385..4528)
                     /locus_tag="NML26_RS27225"
                     /old_locus_tag="NML26_27230"
     CDS             complement(4385..4528)
                     /locus_tag="NML26_RS27225"
                     /old_locus_tag="NML26_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(5196..5387)
                     /locus_tag="NML26_RS27230"
                     /old_locus_tag="NML26_27235"
     CDS             complement(5196..5387)
                     /locus_tag="NML26_RS27230"
                     /old_locus_tag="NML26_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5455..5778
                     /locus_tag="NML26_RS27235"
                     /old_locus_tag="NML26_27240"
     CDS             5455..5778
                     /locus_tag="NML26_RS27235"
                     /old_locus_tag="NML26_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5987..>6424
                     /locus_tag="NML26_RS27240"
                     /old_locus_tag="NML26_27245"
                     /pseudo=""
     CDS             5987..>6424
                     /locus_tag="NML26_RS27240"
                     /old_locus_tag="NML26_27245"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          5024..5111
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4457..5013)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5769..6988
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.754"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaacgcagta agactggtca gggagtaagt gagtatgaca gagctggaaa tgcacttact
       61 gaacgcctta gagcaattgc agcaggacta tacgcagcgg ctgaaagact gggagagcgc
      121 cttcacggat ttgcagcata tgttcgagct gacacgacag gagaacgggc aactgaggga
      181 acagtgcgac gacttgagtc agcaggtgca gagattagcc gggcaggtgg accacttgag
      241 tcggttattt atcacgaaca gcagataagg gatgaacagg aaaggcaggc acgcctggaa
      301 cgggagcgtg cgcggtcctg gcatgaagag caggaacggg aagcacgggt atggcgtggc
      361 ccgtcaatag ggatgtgacg gtgagttgtt ccgggaggtt gccagatggt accggaacgc
      421 gttaaaaacg catccgggcg cgattctggc gtgtcgtttt tgttgctgtg ttttgcctct
      481 ttgccagatc gcgctcattc ctcagcctca tcccgcgatg tctgcgtatg tcatcaactg
      541 aaaagatgga aaaaaaacag tgtcatgagg tttacacagg caaaactagc gttattcaca
      601 tggctgaata gctatgcata cggttaaaat ttatcaggtg cgatcgcggc agcttttcgg
      661 gtggtttgtt gccattttta cctgtcagca gccgtgatcg cgctgaacgc atttcagcgg
      721 tgcgtacaat taagggatta tggtaaatcc cttactgtct gccctcgtat ccatcgagat
      781 aaaccgcaga aatggtgtct gccattagtt tcattttttt cggcgaaggc atatcgctgt
      841 ttgtgtattt tctgccagca aaggctgttg tgctccggag tgagtgaaaa aatttttctg
      901 agtggaattc ttttacacgt tgctgtgccg tttctgctga tactccggct gctttgtctt
      961 tcatccccat atcccggaac tgctgaaaga acgggagcat catacgtatg gtgtctgctt
     1021 tctgggtgta gaagttagga tcaaatccat catctgaagc ataaacataa tgccgggtat
     1081 gtcgttccag gctggtcttt ggtgtgcatc ctgtaagagc tattgttgct gttatcagag
     1141 gtatcagaag cagcctggca gcggcagatt tcataaacat ctccttgcgt ttttaatctt
     1201 gatactcagg gagttatatc tccgaagagt atctcttatc atacgtcggt ttactcccaa
     1261 tgtgatttga tggtcaatga gtcaggaata atcccgtgaa aaaatgtgtg ccagtcctgc
     1321 tttttagtgt tctttctgct tcggttgcca gtcatgcgga cagcaatgtt tttcaacaaa
     1381 aggccgagaa gacaaaaaac ggcattgttg cggatgttaa tgccagacag aaaatgttca
     1441 gtaacagact gaagatacaa cttcagatcg atgagttgag agcagagctg gctggcacca
     1501 gagatgccca aaaaagagag gatttgcgta aaaaactgga gtctctggag cggcaaaaga
     1561 aatccttacg ctgatggggg cgtattttcc ccggtttctt ccataacctt tttaccgttc
     1621 agatatttac gggcgaggcg aatgcgtctt gcctggtata ccgccgagta agcctgggtg
     1681 ggattcacac cattactgat ttcactgacc acatgacgct gatcagccag tgtcagcgtg
     1741 taaacctgtt tctggatgag gtcacgggct ttgtcatgct catatccggc agcaacaagg
     1801 gtgtcgagaa gtcgtttgtc tgttttgttc agtttatggc ggggtgattt gctcacatcg
     1861 ttctccgttt tttcatgatc caacagtttt atatctcatc ctgatttgct ccggtgttct
     1921 gtttcgacgt tctcaatgcc tgcctcccca tagtgtgatc cttattgcaa aaatgaaaat
     1981 ccatgctctt gacatggaca atgctgagta gtaggtttta ctgtatataa aaccagtggt
     2041 tatatataca gtatattgtt tttaatttat tgttttaatt atcaaagagg aatttatggc
     2101 taaagaacta agtggatatg gaccaactgc tggtgagtct atggggggaa caggggcgaa
     2161 tctgaatcag caaggtggaa ataataatag taatagtggg gttcactggg gtggtggctc
     2221 tgggcatggt aataatggtg gtcagggaaa ttcaaattca tctggttcta catcaactgt
     2281 gatgaaaacc ggtgagtcat atctgactcc gtggggtgat gttgtcatta ataatgatgg
     2341 ccttccggtg atgaatggta ttgttatgac agaagagaat tcaactctgg ttgataatcc
     2401 atttggaggg gtctccagag ttcttaattc tttaattagt gatatgccat cactttttgc
     2461 agaaagctca ggaaataaca acaataacac tgcttcagtt aatactgcac caacgaatgc
     2521 tcaggtcagt gatatggata agagttcgaa ggtggtgggt aatgtaatta atgaaaaaca
     2581 aaagcagaag aataaaattg ctacacagat tagtgaaaaa cagaagaaaa tcgaggagat
     2641 gaaaaaagta ttcaagcatc attcttatca tggtatcact gatcttgaga gggatgttga
     2701 tgagttgcaa aaaaaatcaa atcagcttga tgcagatatt agcaaactta attcttataa
     2761 aaatacattg caaagtaaaa ttggtgatgt gaataaacag aaggaagctg aagaaaaggt
     2821 tcgtgaaaat gcagaggtcg ctgaacatga aactcttaat gaagagaaac aggcggtggc
     2881 ggaagcagag aagcgtttag ctgaggcgaa agctgaactg gcgaaggctg aaagtgatgt
     2941 acagagtaag caagctactg tttccagagt tgcaggagag cttgaaaacg ctcagaagag
     3001 tgttgacgtg aaggtcaccg gatttccggg atggcgtgac gttcagaaaa aactacagcg
     3061 tcagcttgaa gctaagcagg ctgagtactc cgcagttgaa aatgaactta agaatgctgt
     3121 tagtttcagg gatggtaagg ccgctgaggt taaagaggca gagcagaaat taaaagaagc
     3181 tcaggatgct cttgaaaaga gccagataaa agatgctgtt gatactatgg ttggatttta
     3241 tcaatatata accgaacaat atggagaaaa atacgcaaaa attgctcagg atttagcgga
     3301 aaagtctaaa ggaaagaaaa ttcaaggtgt tgatgaagct ctggcagcat ttgaaaagta
     3361 taagaatgta ctggataaga aattcagtaa ggttgatcgg gatgctattt ttaatgcgtt
     3421 agagtctgtt aattatgatg agttatctaa gaatttaacg aagatatcaa agtcgcttaa
     3481 aatcaccagt cgagtatctt ttctatatga tgttggttct gattttaaaa atgcaataga
     3541 aactggtaac tggcgacctt tgtttgtcac gctggagaaa agtgcggtcg atgtaggtgt
     3601 ggcaaaaatt gtggcattaa tgtttagttt tattgttggt gtgcctcttg gtttctgggg
     3661 gattgcaatt gtcacaggta ttgtttcttc ttacataggg gatgatgagt tgagcaaact
     3721 taatgagttg ttagggattt aatttctctt tagagtgtaa tatatgaaac ttggcaatcc
     3781 tactgggatt gccattaata aataaaacaa tgcagataac ttgcataagt accagggttc
     3841 tttttttaac tttgtatttt ctttgatata tctagatatg gttaatttag agtatggata
     3901 tagcaataca tgtattgatg atataaatat aaataacaaa ggagtgttat cattctcgtt
     3961 aaatattaaa ataagaatcc atggtataag tgattcaggt aaattatgta ggtagtattt
     4021 taaatgcata agtattttta cttttgttgg aaagaaatat gaaaataata atttttctaa
     4081 ttacaatatt attcttgtca ggatgtcaag caaactatat tcgtgatgta caaggaggaa
     4141 ccgttgcacc atcttcgtct tctgaactga cggggatcgc ggttcagtag aaaagatcaa
     4201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc
     4261 gcctggggag gcggtttagt cgaaggttaa gtcagttggg gactgcttaa ccgggtaact
     4321 ggctttagtg gagcacagat accaaatgct gtccttccag tgtagccgct ataaggcttc
     4381 aacttcaaga gttctgcgta tctaaatcca ctatccagtt accaatggct gctgccagtg
     4441 gcgttaagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4561 aactgagata ccaacagcgt gagctatgag aaagcgccac gcttcccaaa gggagaaagg
     4621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagccaccag
     4681 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4741 gtttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagct gcgtggcctt
     4801 ttccgggttc ttgttttttg ctcacatgtt tcttttggtg ttatcccctg attctgtgga
     4861 taaccgcatt accgtctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4921 gagcgagtca gtgagcgagg aagcggaaaa gcgcctggac gagcattttc tccttacgca
     4981 tctgtgcggc atttcacacc cggcatggtg cacttttcat acaatcctca ctgatgccaa
     5041 atgattaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     5101 cacccgctaa aacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac
     5161 aggccagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg
     5221 aagcgtgcga ggcagctgcg gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg
     5281 tcagcctggt cgtcggcgtc cagttcattc agtttttcca gcagggtcag cgtctggctt
     5341 ctgatgaagc gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc
     5401 catgtgtggg gagtctgtcg gaggttttta atgataccgg tgaaacgcga aaggatgctc
     5461 acgatacggg tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa
     5521 cttgcgtcat ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt
     5581 ccgacactgg caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag
     5641 atagcccgga agataaacag cggacagtgg tcggggcatg accgggttca tgtggtggct
     5701 gcactgatgg ccatcggacg tgaactgagt gagctgaggg atgaggtccg taaacaggga
     5761 gaacgggatg atagttaagt ttcatgccag gggaaaaggc ggtggcagtg gtccggttga
     5821 ttacctgctg ggccgggagc gtaaccggga aggggcgaga gttctgcggg gtgctcccga
     5881 agaggtgcgg gaactcatcg atgccacgcc gtttgcgaaa aaatacacct cgggggttct
     5941 gtcttttgca gagcagaccc tgccaccggg agagcgtgaa agggtgatgg agagctttga
     6001 gcgggtactg atgcccggtc ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca
     6061 ggacaagggg cgtcttgagc tgaattttgt catcccgaat atggagctgg cgagcggaaa
     6121 acggctccag ccctattacg accgagcaga ccgtccgcga atcaatgcct ggcagacact
     6181 ggtgaatcac cattacgggc tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt
     6241 caccccgaat aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact
     6301 ggaggctctt taccatgccg gagcactaaa aacgcgtcag gatgtcactg aggcgcttac
     6361 agcggcaggt tttgaggttg tgaggacaac caggagcagt atcagtatcg ccgacccgga
     6421 gggtgggcgt aatctcaggc tgaaaggggc gctgtatgag caatctttta caaatgggga
     6481 cagacttcga gaagaaactg aaagagcgtg ccgtctctac agagaacatg ctgaacagcg
     6541 agttcaggaa gctcgaagcg tctgtagaca gggaactgaa cttaaacggg cagaaaatca
     6601 gagacgctat cagcgcacac acagcagcgg tgaaagaaca actggagacg ctgaacagtg
     6661 ccgtgaacgc gcagttcagc gccacagaga acgaactggc ccggcggcag gaagagctgc
     6721 tctggaagct ggtcaaaggg cggatactgt acccgtcgct gacggcgctt tgcgtcacgg
     6781 gcgggatttt tctggcgtca tgggggctga ttcagtggca ggaaagccgg attgcggcga
     6841 acatcctggc tatcagggac caggaagaga cgctggcgaa actccgggaa aaaacatggg
     6901 gtgtgacgta tcacgagggc aggaacggga aatttctggt gttgccgtcc ggggtgaagg
     6961 gggaaaacaa ctggacggtg gagaagaa
//
