LOCUS       NZ_CP101208             2089 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-151 plasmid pEC21Z151-2K-1, complete
            sequence.
ACCESSION   NZ_CP101208
VERSION     NZ_CP101208.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593415
            Assembly: GCF_024298745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,300
            CDSs (total)                      :: 5,176
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 82 of 223
            Pseudo Genes (incomplete)         :: 162 of 223
            Pseudo Genes (internal stop)      :: 45 of 223
            Pseudo Genes (multiple problems)  :: 57 of 223
            Pseudo Genes (short protein)      :: 3 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101208.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-151"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z151-2K-1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-15"
     gene            complement(504..770)
                     /locus_tag="NML27_RS26455"
                     /old_locus_tag="NML27_26450"
     CDS             complement(504..770)
                     /locus_tag="NML27_RS26455"
                     /old_locus_tag="NML27_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(774..1739)
                     /locus_tag="NML27_RS26460"
                     /old_locus_tag="NML27_26455"
     CDS             complement(774..1739)
                     /locus_tag="NML27_RS26460"
                     /old_locus_tag="NML27_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1115..1346)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 accttttcca cacacccgat cactaaaaca gggaataaaa acaaaaccct gtcttacagg
       61 tggatggcct cacttcgtga aaccatccac cccgcataag ttttttaatc ggtggtgtcg
      121 tcaatcacta aatcggaacc ccaaagggga cccccacgat ttagcatttg acgagattaa
      181 ctctcggaga tggcatcatg cttttaacgg actttaaaaa acggtgaacg gatgcggatt
      241 ttaatccgtg tctgtgaggc gttgagcaac gagcaaagcg aggcggtaga aagagagcca
      301 gcggcaggct caggacgata atccggggaa aatgtaccac tcgcagagtg ttaacatttg
      361 acatcggatt gtctatcctg cattggtgtc gagttggcaa ggctgaggat aatgctgagg
      421 ataatgcagg atagacagag cactagagat tgcatgcaat ctcgtaccta tcacctcagc
      481 ctgccgctgg ctcttttcca gaactaagaa tgcgttctta aaaatccaaa cttgataccg
      541 tttaaatcta ttttcacaaa aagagttttt accctctcgt ttgttttctg aatatataaa
      601 attaattcat cctcaaaacc ttcgtgcgga tgaaaaccaa atgtgccaaa acttaatcca
      661 tcaataagca aaataaggtt tttaaacctg tcaggaatca ccgcttcact tcttaaaaaa
      721 aataaaccaa tagtcttatt tccattcagg tattcaatat taatattcat acgttaatcc
      781 gatttatcct tctcaggggc gcgtttgtaa cgccgtttac ctgaatccca gacaaacgcc
      841 gtccgcttcc cgtcatcagt gccatcccct acatcgtcgg caatgacaag atcctcattg
      901 gtttctcgat ccaactggag gacgtttttt agcgccccac cggtcgagat aaaacggcgc
      961 ttgtgaagct gccgcgtcag ctcaagaaac cactcaggat cgtttgccat atcttccggt
     1021 ttaacggagt atttcagcgt ttcagcaacc gcgctttgca gttgctcggc aacgttggcc
     1081 acaacctcac ctgtcttagt ttttactgcc cgaatatcga tattcggctc atagttcacc
     1141 cgcaagcaat cgcgccagag ttctacccaa cgttcgtgct taacgtagtt cttcccttta
     1201 aaccaagaag gttgcaccat cagcagacag tgaaaatgcg gatgtgcgct gccatcctta
     1261 cctcgcgtca cctccgtagc cctgatccac ccctgaacag gtgatagctc ctttcgcttt
     1321 tccattcgct taaacgccgc attcattgct gtaaggactg ttccaagttc acctatctcg
     1381 cagttcctga cagttaacgt cagaaacaac catcgggaag acgggtaatc cacaacgatt
     1441 ttcggtagag cctgataaaa acgggcttgc cacatgaggg ttcttctcca ctggcagaca
     1501 gggcaatgac ggacacggca aaattgcgca ctacgaagcc gtaggcgcgt ttctccggtt
     1561 tccatgatgg tagaccaacc aaaacgtaac aaaccgctac aggaggccat acggagtgca
     1621 tagcgctcga attcagcagc agtctggtag atacccccga cagaatcggt aagcgtgcgc
     1681 tgggtatccc aaactgcatc acgggggctg tagtccgaaa ggaatttatc ttctgacatt
     1741 aagcctgctc cgatctgggc aggctgtgat ttgatgtggt atattcatca cgtacctgtg
     1801 cattgcgagc cagtacatgt caccagagtt tgccgctgag gtgacacatt cgaaatgttg
     1861 gggagtgttg acgcactccc caacgccgat taaaaacgcc tcaattctgc tccattttct
     1921 ttgacctgtc aaaacccact caaagcctcg tcattactgg gctaaaccac tatttcatat
     1981 ccgccattta tcccttaagt ataaatacaa ggatcacgcg cgagaattgc gcaaaaaata
     2041 accacaattt taacccccca aaacaagcac ccccgaacgg cttacccca
//
