LOCUS       NZ_CP101207             4072 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-151 plasmid pEC21Z151-4K-2, complete
            sequence.
ACCESSION   NZ_CP101207
VERSION     NZ_CP101207.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593415
            Assembly: GCF_024298745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,300
            CDSs (total)                      :: 5,176
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 82 of 223
            Pseudo Genes (incomplete)         :: 162 of 223
            Pseudo Genes (internal stop)      :: 45 of 223
            Pseudo Genes (multiple problems)  :: 57 of 223
            Pseudo Genes (short protein)      :: 3 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101207.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-151"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z151-4K-2"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-15"
     gene            join(3796..4072,1..416)
                     /locus_tag="NML27_RS26440"
                     /old_locus_tag="NML27_26435"
     CDS             join(3796..4072,1..416)
                     /locus_tag="NML27_RS26440"
                     /old_locus_tag="NML27_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(672..2831)
                     /gene="mobQ"
                     /locus_tag="NML27_RS26445"
                     /old_locus_tag="NML27_26440"
     CDS             complement(672..2831)
                     /gene="mobQ"
                     /locus_tag="NML27_RS26445"
                     /old_locus_tag="NML27_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3010..3264
                     /gene="mobC"
                     /locus_tag="NML27_RS26450"
                     /old_locus_tag="NML27_26445"
     CDS             3010..3264
                     /gene="mobC"
                     /locus_tag="NML27_RS26450"
                     /old_locus_tag="NML27_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2474..2832)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          2833..3009
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          73..416
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.74e-180"
     Typing          3797..4072
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.473"
                     /coverage="100.0"
                     /evalue="1.0900000000000001e-130"
     Typing          complement(676..2918)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctggatcca gtatcgggca cagtacgcta aaggtgaggc caaggctcga ataacctttt
       61 ctgacgcggt aatgccttat ctgacgcaac tgaaggggca attcacgcgc gttgttataa
      121 aaaacatatc aggtttatca agtagttaca gtataagaat atacgagttc ctccagcaat
      181 tccgcagtac gggagagaga attattgctc ttaatgactt tcgttcaatg ttagggattg
      241 aaaataaata caaacaattc agagacttaa ataaaatact aataaaaccc tgtgttgacg
      301 aactgaacaa aaaaagcgac ctggctgtaa cggttgagac gatcaaaaag gggcgcacgg
      361 ttgtagccct gcattttcgc ttcaaggaag acaaacagat caagatgacg atctaaaatc
      421 gattttaagg cacctaaaac gctccctgct gcgttttttt tgtgttagta gtgcgaaggt
      481 actattgaag tgaaaaattg cgtcaaaagc gctctaatgc agcgaaaaaa gaacccccaa
      541 tttttggggg cggaaaaaca aaactatgct tgcattatgt gtgtgcttct tgcattcatc
      601 cctgtcgatg aacatagaag aacatcgacg aacataacac gatgttaaac aaaacaaaat
      661 attgttcaca ttcactgtga ccaaccgcga tcatgtctcc tctcggtggt gtgtgttctt
      721 gcatcttcct cgcgccgcca ttcatcccag gtacgattga aacccagaga gcggaacaga
      781 tgatccggct gtttttctgg tgtgttgacg gcagcaaaaa gcggtgcctc acgggttttc
      841 agatgaaact ccataagttc cggcgcaggt gatatgtggc tgtctgcatc gtgatttacg
      901 gcaacaccat accctgcata ccgtaacccc ttatcggtca gtgcactgat gaccagctgg
      961 ctgttatcgg ctgagggaac ggcaaacgcg ggatacagcg taccattaag ccggacaaca
     1021 ccgcccagag agtcatccac gcccatgacc agcaaaagct gaagctggcg ggtttcccgg
     1081 tcgacagaac tcattaccgg catcagcgtc ggtagcagac gtccctgcgt aaaatccacc
     1141 ttccttgctg ccagtgatgg catggctggc tggcggtctt ctccaagaca gaaagactcc
     1201 ggccatgtct tcggctggcg gagattgcgg gtatattgcg gccatgtctg gcaaacctgc
     1261 gtcagaagtt gctcaaaggt cgcagaggaa taaacacgtt gcagtggtgg atgttgaccg
     1321 atttcgcttc ggtcacagat ataaccactc ctgtcacgca ttaaatcaac acgctcaaag
     1381 attctggtgc ggtatttctc cagctcgttc ccccagagcg tgtattctcc gttccggttg
     1441 tgaagcagaa ggtaacaggt catttccccg ttaccaggca tgggggcctc gccatacccc
     1501 gtaacgactg cgctcagtac ccctgaatcc ataacggcat tgtcattttt cctgagctgt
     1561 tccgctcgtc gctccgcgcg actacgttct gtatcctcct gctccttccg gcgcaattcc
     1621 tcttcacgtt taagcaactg gactctgaga gctgcaaggc cggatgcccc ccactgctgc
     1681 cactgttttt caaatgccgt ctgactcgcg ggaatgtcct gtttctccac cttccggtaa
     1741 tgctgtcgca atttttccat ttgctggctc agcgactgga tttttccctg ttcgctacgg
     1801 tattccggtg ccgcccattg catcagggga atacgggaaa aaccggacga aaacggcgat
     1861 gattttttca ggctggcagc atggctcatc gcttcctttc gttcggtgtt cagctcttcc
     1921 cagcgtctgc gcgtcgcctg catatctgcc atcatcgaca tggcattact ggtgagtttc
     1981 catgccgcat tactgtcctt cggcaacgct tcgatgatgt cacgtagtgt gtacgaagtt
     2041 gcgttgtcag gaagcacgat attcagaagg tcagccacac ggcgggcagg ctctgtcagc
     2101 ccgctgttcc tgagttcagt gcgggaaatc tgtatttcca ggttaagcct gttaatcaga
     2161 cgattctgtt cgccgcgttc tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt
     2221 ccgaggtgaa taccaggcgt tttctcaagc ccctgtcgct cgtatgaacg atggtctatc
     2281 tcttcctgat aaccggcgtt cgccagtgct ctgtttgcat ggtcagccca tgatgcacgc
     2341 cacgtctcgg catgtgtccg gtcgttccag tccctgacct ttccgccgaa cccagcaggt
     2401 ccgacagccc tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg
     2461 aacgccacat cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg
     2521 gtttcccgtg cggcatcctg cggcagttca cgggggatag cgagttcaat ttcccgcgca
     2581 agttgtgagt tcttccggca ttcggccttc tcaacggcat tccagagttc ggcacggttc
     2641 gtacaccagg caggggctgt cgcgggggtc agaatcacag cttcagccac accgcttttg
     2701 cgggtgtagt cgtgagtcag tccggtacgc tcatcaagca tcagtgaacc ggaacgatag
     2761 gcagcggaag ctacggcgct gtagccgctg cttctggaga taattttcat gctgagatga
     2821 taaatcgcca tttctgccgt aatgcctttt tgtagttttc tttcagcccc gcagggcgca
     2881 cacaatcgaa acggagtgga gattgtataa gtgcgcactt tgtgatttgt cgcgcattat
     2941 gccagctcag tttttaaact accagtgacg cgctaaaatg cgatttttca tcgcgcacgg
     3001 agttacatca tgagcagaac gctcgaacag aagattgctg atgctgaggc gagattacag
     3061 cgcctgaagg cgaagagccg gagcctggac acagcgcaga aggttgttgt gggtgcagca
     3121 ttgctggcaa aggtcagaaa gccggaggag gtgcagttgc gtgcctggtt actccagttc
     3181 ctgaaggcag aggtgacacg acaggctgat gtgacgcgca tactgccgct gattaacgag
     3241 ctggaggcgc tgccaggaca gtgattgtat cgtccgctcg ccgcagcccc agcgaccgag
     3301 cgttagcgag cgagtgggcg aggaagcgga agtgaatccg tcgccaggtt gagcacatac
     3361 gcacggtttt tcggtaggtg atatgcgctg aacccgcatg aatactggat ttccacaggg
     3421 ttggaagggg agtattcgga acttgcaatt ctgctttctt gcaacacatt gtttttattg
     3481 gttttttgtt ttcagcgccc caagttatcc acaggctcgc cgttaagttt ttagatcttg
     3541 tttttaagat cttgttttta atacttgttt tagggtcaaa attcattaat aaaatcaata
     3601 tgttatagag gtatatgtgg aggaatttaa ggtaaaggtg gaggaattta aggtgtttag
     3661 gtggaggaat ttaaggtaaa ggtggaggaa tttaaggtgt tattcacaga aatgtgagga
     3721 tataaaaaaa aatattccac atttcttatt tgtggggcac attaaagcat caccacattt
     3781 gaacgggatc tttgtatgtc ggacttgatt gcctacaagt cgaatgcttt ggttgaggcc
     3841 agctacaagt tgaccctaca ggaacaacgt tttctgttgc tgtgtatcag tcgattgaaa
     3901 tctggtgctg atgctgaatt gcaaaagaca atgaccatta ccgctgcgga atattttgat
     3961 tcattccctg acatggggcg gaaaaatgcc gaggttcagt tacaggaagc gatcgatcgt
     4021 ttatgggaca ggtcgatcat tctcaagaat gacgagaagc gtgaggagtt cc
//
