LOCUS       NZ_CP101206             4091 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-151 plasmid pEC21Z151-4K-1, complete
            sequence.
ACCESSION   NZ_CP101206
VERSION     NZ_CP101206.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593415
            Assembly: GCF_024298745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,300
            CDSs (total)                      :: 5,176
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 82 of 223
            Pseudo Genes (incomplete)         :: 162 of 223
            Pseudo Genes (internal stop)      :: 45 of 223
            Pseudo Genes (multiple problems)  :: 57 of 223
            Pseudo Genes (short protein)      :: 3 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101206.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-151"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z151-4K-1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-15"
     gene            complement(join(3409..4091,1..1465))
                     /gene="mobQ"
                     /locus_tag="NML27_RS26425"
                     /old_locus_tag="NML27_26420"
     CDS             complement(join(3409..4091,1..1465))
                     /gene="mobQ"
                     /locus_tag="NML27_RS26425"
                     /old_locus_tag="NML27_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_011666798.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     KRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            1636..1926
                     /locus_tag="NML27_RS26430"
                     /old_locus_tag="NML27_26425"
     CDS             1636..1926
                     /locus_tag="NML27_RS26430"
                     /old_locus_tag="NML27_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            2459..3160
                     /locus_tag="NML27_RS26435"
                     /old_locus_tag="NML27_26430"
     CDS             2459..3160
                     /locus_tag="NML27_RS26435"
                     /old_locus_tag="NML27_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          1111..1465
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1482..1632
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          2460..3159
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..1507)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.801"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3413..4089)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="5.91e-140"
ORIGIN
        1 cacgcattaa atcaacactc tcaaaaattc tggcgcggta tttctccagt tcgttccccc
       61 agagcgtata ttctccgttc cggttgtgaa gcagaaggta acaggtcatt tccccgttac
      121 ctggcatggg ggcctcgcca taccccgtaa cgactgcttt cagtacccct gaatccataa
      181 cggcattgtc atttctcctg agctgctccg cccgacgacg ttctgtttcc tcctgctcct
      241 tccggcgcaa ttcctcttca cgtttacgca actgttctcg gagatctgca aggccagatg
      301 caccccactg ctgccactgt gtttcaaatg ctttttgact cgcgggaatg tcctgtttct
      361 caaccttcca gtgatgctgc cgcaattttt ccatgcgctg gctcaacgac tggatttttt
      421 cctgttcgcg gccgtattcc ggtgcaaccc attgcatcag gggaatacgg gaaaaaccgg
      481 acgaaaacgg cgatgatttt ttcaggctgg cagcatggct catcgcttcc tttcgttcgg
      541 tgttcagatc ttcccagcgt ctgcgcgtcg cctgcatatc tgccatcatc gacatggctt
      601 tactggtgag tttccatgct gcgttactgt ccgtcggcaa cgcttcgatg atgtcatgca
      661 gtatgtacga agttgcgttg tcaggaagct caatattcag aaggtcagcc atacggcgtg
      721 cgggctctgt cagctcactg ttcctgagtt cgtcgcggga aacctgtatt tccaggttaa
      781 gcctgttaat caggcgattc tgttcgccgc gttctgtttc gatgccccac ttttccatcg
      841 cacaggccga ctttccgagg tgaatgccag gcgttttctc aaccccctgt cgctcgtatg
      901 aacgatggtc tatctcttcc tgataaccgg cgttcgccag cgccctgttc gcatggtctg
      961 cccatgatcc ccgccatgtt tcggcgtgag tccagtcatt ccactcacgg actttctccc
     1021 cgaagccgtc aggcgtgatt gccctcatgg atagcatgat gtgcgcgtgt gggtttgacc
     1081 cgcccagatt gtgaaaggcc acgtcggcga tcatgccgcg actgacaaag ttttccctta
     1141 cgaagttgag aactgtttcc cgtgcggcat cctgcgccag ttcacggggg atggcgagtt
     1201 cgaactcccg cgcaagttgt gagtttttcc gccgctcggc tttttcaaca gcattccaga
     1261 gttcggcgcg gttcgcacac catgctgggg cgtttgctgg cgtcagaatc actgcgctgg
     1321 ctacaccgtt tttgcgggtg tagtcgtgaa tcactccggt gcgctcatcc agtatttttt
     1381 ctccggcacg ataggcagca gaagcaacgg cgctgtagcc cttgcttctg gaaagaatct
     1441 tcatgctgag atgataaatt gccatcgtgt cgttgttgcc gttttctttt agccccgcag
     1501 ggcgcacaca aactcgacga aggagagttt gtataagtgc gcacttcgtg atttgctgtg
     1561 cattatgcca gttcagtttt taaactacca gtgacgcgct aaaatgcgat ttttcaccgc
     1621 gcacggagtt acggaatgag tagaacactc gaacagaaga ttgccgatgc tgaggccaga
     1681 ttgcagcgtc tgaaggcgaa gagcaggagc ctggacacag cgcagaaggt cattgtggga
     1741 gcggcattgc tggcaaaggt caggaagcct ggggaaacac aactacgagc ctggttgctt
     1801 catctcctga agacagaggt aacacggcag gctgatgtga gcaggataca gccgttgatt
     1861 gacgaactga acgcgttacc aaaaccagtc cccaaagaag tgtcagaaaa cgaccaacag
     1921 gcgtgaatgc accgtccgct cgccgcagcc ccagcgaccg agcgttagcg agcgagtggg
     1981 cgaggaagcg gaagtgaatc cgtcgccaga ttgagcacat acgcacggtt tttcgacagg
     2041 tgatatgcgc tgaacccgca tgaatactgg atttccacag ggttgagcgg ggttttgcta
     2101 aagcttgcga acctgctttt ttgtaacctg ttgtttttca tgattttttg ttttgaacac
     2161 cccaagtttt ccacaggctc gccgttaagt ttttagatct tgtttttaag atcttgtttt
     2221 taatacttgt tttagggtcg aattttctta tgaaattcaa gagtttagaa gactataggt
     2281 aatataattt aaggtaaagg caatatgatt taaggtggat aggcaatatg atttaaggta
     2341 aaggcaatat gatttaaggt aatattcaca ggaaaaggga tattaaaatt aaatcatatt
     2401 gcctttacct aaaggaatgt ttatatttta ctcattacct taggttgagg gatttacaat
     2461 gtcggatctg attgcctaca aatccaatgc actggttgag gccagctata aattgactct
     2521 tcaggaacag cgattcttgt tactttgcat tggtcgattg aagtcgggtt ctgatgcaga
     2581 atccactgaa ttgcaaaaga ccatgactgt taccgccgaa gagtatttcg actcattccc
     2641 tgatatgggg cggaaaaatg ccgaggttca gctacaggaa gcaattgatc gtttgtggga
     2701 caggtcgatc atccttaagg acgatgagaa gcgcgaggag ttccgctgga tccagtatcg
     2761 ggcacagtac gctaaaggtg agggcaaagc ccaaatcact ttttcagatg cggtcatgcc
     2821 ttatctgaca cagttgaagg ggcaatttac tagagttgta attaagaaca tctccaattt
     2881 atcaagaagt tacagcataa gaatatacga acttctccag caattccgca gcacagggga
     2941 acgtattatc gccctcgatg attttcgctc aatgcttgat atagagcata aatatcaaac
     3001 atataagtct ctgaaccagc aattattaag accttgcgtt gacgaactga atcaaaaaag
     3061 cgacttggct gtaacgcttg agacgatcaa gaaggggcgc acggttgtag ccctgcattt
     3121 tcgcttcaag gaagacaaac agatcaagat gacgatctag aatcgatttt aaggcctcta
     3181 aaacgctctc tgacgcgttt tttttgtgtt tggcatgcga aggtactggt gaagtaaaaa
     3241 attgcgtcat aaacgctctg gcacagtgaa aaaagaaccc ccaatttttc gggggcggga
     3301 aaactataaa gttgatttgt gttatgtgtg tgcttcttgc attcatccct gtcgatgaac
     3361 atcgacgaac ataacacgat gttaaacaaa acaaaatatt ggtcacattc actgtgacca
     3421 accgcgatca tgtctcctct cggtggcgtg tgttcgggca tattcatcac gtttccattc
     3481 atcccaggtg cgctcaaagc ccagcgagcg gaacagatga tccggctgtt tttctggcgt
     3541 gttgacgata gcaaaaagcg gttcgctcca ggttttcaga tgaaactgca taagttccgg
     3601 cgcaggacgt gccggactgt cagcatcgtg gtttaccgct tcaccatagc ctgcaaagcg
     3661 taaacctttg tcggtcagtg cattgattac cagctggctg ttgtcggctg tggggacggc
     3721 aaacgcggga tacagcgtac cattaagccg gacaacaccg cccatagagt catccacacc
     3781 catgaccagc aaaagctgaa gctggcgggt ttcacggtcg acagaactca ttaccggcat
     3841 cagagtcggt ggcagacgtc cctgcgtaaa atccaccttc cttgccgcca gtgatggcat
     3901 ggctggctgg cggtcttctc caagacagaa agactccggc catgtcttcg gctggcggag
     3961 atcgcgggta tgttgcggcc atgtctggca aacctgcgtc agcagttgct caaaggtcgc
     4021 agaggaataa acatgttgca gtggtggacg ttgaccgatt tcgcttcgct cacagatata
     4081 accactcctg t
//
