LOCUS       NZ_CP101204             6648 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-151 plasmid pEC21Z151-7K, complete
            sequence.
ACCESSION   NZ_CP101204
VERSION     NZ_CP101204.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593415
            Assembly: GCF_024298745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,300
            CDSs (total)                      :: 5,176
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 82 of 223
            Pseudo Genes (incomplete)         :: 162 of 223
            Pseudo Genes (internal stop)      :: 45 of 223
            Pseudo Genes (multiple problems)  :: 57 of 223
            Pseudo Genes (short protein)      :: 3 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101204.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-151"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z151-7K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-15"
     gene            join(6571..6648,1..1491)
                     /gene="cea"
                     /locus_tag="NML27_RS26355"
                     /old_locus_tag="NML27_26350"
     CDS             join(6571..6648,1..1491)
                     /gene="cea"
                     /locus_tag="NML27_RS26355"
                     /old_locus_tag="NML27_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1877..2014
                     /locus_tag="NML27_RS26360"
                     /old_locus_tag="NML27_26355"
     CDS             1877..2014
                     /locus_tag="NML27_RS26360"
                     /old_locus_tag="NML27_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3041..3232)
                     /locus_tag="NML27_RS26365"
                     /old_locus_tag="NML27_26360"
     CDS             complement(3041..3232)
                     /locus_tag="NML27_RS26365"
                     /old_locus_tag="NML27_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3296..3619
                     /locus_tag="NML27_RS26370"
                     /old_locus_tag="NML27_26365"
     CDS             3296..3619
                     /locus_tag="NML27_RS26370"
                     /old_locus_tag="NML27_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3828..>4259
                     /locus_tag="NML27_RS26375"
                     /old_locus_tag="NML27_26370"
                     /pseudo=""
     CDS             3828..>4259
                     /locus_tag="NML27_RS26375"
                     /old_locus_tag="NML27_26370"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4297..4815
                     /locus_tag="NML27_RS26380"
                     /old_locus_tag="NML27_26375"
     CDS             4297..4815
                     /locus_tag="NML27_RS26380"
                     /old_locus_tag="NML27_26375"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4822..5055
                     /locus_tag="NML27_RS26385"
                     /old_locus_tag="NML27_26380"
     CDS             4822..5055
                     /locus_tag="NML27_RS26385"
                     /old_locus_tag="NML27_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5373..5792)
                     /locus_tag="NML27_RS26390"
                     /old_locus_tag="NML27_26385"
     CDS             complement(5373..5792)
                     /locus_tag="NML27_RS26390"
                     /old_locus_tag="NML27_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5873..6310)
                     /locus_tag="NML27_RS26395"
                     /old_locus_tag="NML27_26390"
     CDS             complement(5873..6310)
                     /locus_tag="NML27_RS26395"
                     /old_locus_tag="NML27_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          2869..2956
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2300..2855)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3610..5159
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtactcctg acgggagtgg ctctggcggc ggaggtggaa aaggaggcag taaaagtgaa
       61 agttctgcag ctattcatgc aactgctaaa tggtctactg ctcaattaaa gaaaacacag
      121 gcagagcagg ctgcccgggc aaaagctgca gcggaagcac aggcgaaagc aaaggcaaac
      181 agggatgcgc tgactcagcg cctgaaggat atcgtgaatg aggctcttcg tcacaatgcc
      241 tcacgtacgc cttcagcaac agagcttgct catgctaata atgcagctat gcaggcggaa
      301 gcagagcgtt tgcgccttgc gaaagcagaa gaaaaagccc gtaaagaagc ggaagcagca
      361 gaaaaggctt ttcaggaagc agaacaacga cgtaaagaga ttgaacggga gaaggctgaa
      421 acagaacgcc agttgaaact ggctgaagct gaagagaaac gactggctgc attgagtgaa
      481 gaagctaaag ctgttgagat cgcccaaaaa aaactttctg ctgcacaatc tgaagtggtg
      541 aaaatggatg gagagattaa gactctcaat tctcgtttaa gctccagtat ccatgcccgt
      601 gatgcagaaa tgaaaacgct cgctggaaaa cgaaatgaac tggctcaggc atccgctaaa
      661 tataaagaac tggatgagct ggtcaaaaaa ctatcaccaa gagccaatga tccgcttcag
      721 aaccgtcctt tttttgaagc aaccagacga cgggttgggg ccggtaagat tagagaagaa
      781 aaacaaaaac aggtaacagc atcagaaaca cgtattaacc ggataaatgc tgatataact
      841 cagatccaga aggctatttc tcaggtcagt aataatcgta atgccggtat cgctcgtgtt
      901 catgaagctg aagaaaattt gaaaaaagca cagaataatc tccttaattc acagattaag
      961 gatgctgttg atgcaacagt tagcttttat caaacgctga ctgaaaaata tggtgaaaaa
     1021 tattcgaaaa tggcacagga acttgctgat aagtctaaag gtaagaaaat cggcaatgtg
     1081 aatgaagctc tcgctgcttt tgaaaaatac aaggatgttt taaataagaa attcagcaaa
     1141 gccgatcgtg atgctatttt taatgcgttg gcatcggtga agtatgatga ctgggctaaa
     1201 catttagatc agtttgccaa gtacttgaag attacggggc atgtttcttt tggatatgat
     1261 gtggtatctg atatcctaaa aattaaggat acaggtgact ggaagccact atttcttaca
     1321 ttagagaaga aagctgcaga tgcaggggtg agttatgttg ttgctttact ttttagcttg
     1381 cttgctggaa ctacattagg tatttggggt attgctattg ttacaggaat tctatgctcc
     1441 tatattgata agaataaact taatactata aatgaggtgt tagggattta attattcttt
     1501 attgatataa aaagtcctag caatccaaat gggattgcta ggaccaaaca aagtagatta
     1561 tatagcataa ataggtttaa ttttgctacg ggggcgttat ttaggttttt tcttctttcg
     1621 aaaaaatctt tctttatgaa gttaaaagct atgtgttcaa tagcatattt tgaatatgga
     1681 catagaatag tgcttatcac tattgcatat agcatcttat ctgacacaag gaaataatac
     1741 ccttcgctgt tttttgttat aaggtatata tatataagtg tgcagtacag gccaaataaa
     1801 atatttttta tgtagtatct taagctcata aattaaacct cgccatatat tcttttcatt
     1861 ttataaggat cgagttatga ggaaaagatt ttttgtggga atattcgcga taaacctcct
     1921 tgttggatgt caggctaact atatacgtga tgttcaggga gggaccatcg caccatcctc
     1981 ctcttctaaa ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat
     2041 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accaacggtg
     2101 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2161 gcgcagatac caaatactgt ccttctagtg tagccgtagt cgggccacta cttcaagaac
     2221 tctgtagcac cgtttgtgcc atcatcgctc tgctaatccg gttaccagtg gctgctgcca
     2281 gtggcgttaa ggcgtgcctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2341 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2401 ccgaactgag ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2461 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2521 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2581 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc tgctacgtgg
     2641 ccttcttcct gttcctggtc ttttgctcac atgttctttc cggccttatc ccctgattct
     2701 gtggataact gtgttaccgt ttttgtgtga gtcagtaccg ctcgccgcag tcgaacgacc
     2761 gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc tggacgtgca ttttctcctt
     2821 acgcatctgt gcggcatttc acacccggca tggcgtactt ttcatacaat ccgcactgat
     2881 gccgcatggt taagccagta tacactccgc tatcgctacg tgactgggtc agggctgcgc
     2941 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtcagctc ccggcatccg
     3001 ctcacagaca agctgtgacc gtctccggga gctgcatgta tcagaggttt tcaccgtcat
     3061 ccccgaaacg tgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac
     3121 aaatatcggc ctgttcatct gcgtccagtt cgttgagctt ctccagcagc gttaatgtct
     3181 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttagtc actgatgcct
     3241 ccgtgtaagg gggatttctg ttcatggggt aatgataccg atgaaacgcg agaggatgct
     3301 cacaatacgg gttactgatg atgaacatgc ccggttactt gaacgctgtg agggtaaaca
     3361 actggcggta tggatgcggc gggtctgcct gggggagccg gttgcccgtt ccggaaaact
     3421 gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcggaaata acctgaatca
     3481 gacagcccgt aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc
     3541 cgcactgatg gccatcgggg atgagctgcg ccggctgcgt ctggccgtca gggaacaggg
     3601 ggcgcgggat gatagttaaa tttcatgcca ggggaaaagg tggtggcagt ggtccggttg
     3661 attacctgct ggggagggag cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg
     3721 aagaagtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc
     3781 tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg
     3841 agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag catcctgtgg gtggagcacc
     3901 aggacaaggg acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa
     3961 aacgcctcca gccgtactac gaccgcgcag acaggcctag aattgatgcc tggcagacgc
     4021 tggtgaatca ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc aggacgctga
     4081 cactccctga taacctgcct gaaacgaaac aggcgcttgc tgagggcgtc acgcgaggta
     4141 tagatgcact ttaccatgcc ggagagataa aaggccgtca ggatgtgatt caggcgctca
     4201 ctgaggcggg gctggaagtg gtcagggtga cgcgaagcag tatcagcatt gcagatccga
     4261 acggcgggaa gaatatcagg ctgaaaggag cattttatga gcaatctttt gcagacgggc
     4321 gcggagttcg agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacaac
     4381 gagttcagga ggctcggcga atctgtaagc gaggctgtga catcaaacga gacgaaaatc
     4441 agagacgcta tagccctgtt cacagcctcg acagaggaat cgctggaaaa acaccgggaa
     4501 ggggtgaaag aggcgatgat gcagcacagg agggacgtgt taaagctggc agggaatacg
     4561 ggcatgatgt tactggggat agtctttctc ctgtttaccg cgagtggcgg gacgctctgg
     4621 tatcttggag ggaggataca ggcgaacctg gaagaaatca ggaagcagga agagacattg
     4681 cagaaactga acgcgaagac atggggcgtg gagtttgtgc aggacgggaa caggaaattc
     4741 cttgtccttc cgtacgggaa atcagcggag gtgattccct ttcaggggaa agagtgggta
     4801 catctgaagg agtgacacag agtgacagag ctggaaacac atttgctgaa cgccttagag
     4861 cagctgcaac aggactatat gcagcggctg agcgaatggg agagcgcctt cgtggaattg
     4921 cagaagatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag
     4981 ttgagtcagc aggtgcagca cttgagcgag cagacagaac gcttgagcca gttatacagc
     5041 gagaactgga gataagagag gaacggctga tacaggagcg cgaacatgtg ttatccctgg
     5101 aacgggagcg tcagccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     5161 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     5221 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgatcgcggc agtttttcgg
     5281 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     5341 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     5401 taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag gtctttgctg
     5461 cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa ttcctggctg
     5521 tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc tttatcttta
     5581 gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt gtcggctttt
     5641 tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg ccgggtatgt
     5701 tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt tatcatggcg
     5761 ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta aagtatttat
     5821 tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc gttcagcgac
     5881 cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc ctgagctggg
     5941 ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc atatcctgtg
     6001 cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagc tcccggagaa
     6061 tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc ctttctggtg
     6121 tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca aactcccgtc
     6181 gtccgtccct gccggtacgg taactgacaa ggcctgcgcg catatcacgg taaaactgtg
     6241 aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc ataaagtgac
     6301 agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt ctgagaacgg
     6361 taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt gaaagactgt
     6421 ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa atgcagcggc
     6481 gtagcttttt atgctgtata taaaaccagt ggttatatgt acagtattta tttttaactt
     6541 attgttttaa aagtcaaaga ggattttata atggaaaccg cggtagcgta ctataaagat
     6601 ggtgttcctt atgatgataa gggacaggta attattactc ttttgaat
//
