LOCUS       NZ_CP101202            24263 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-151 plasmid pEC21Z151-24K, complete
            sequence.
ACCESSION   NZ_CP101202
VERSION     NZ_CP101202.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593415
            Assembly: GCF_024298745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24263)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24263)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,300
            CDSs (total)                      :: 5,176
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 82 of 223
            Pseudo Genes (incomplete)         :: 162 of 223
            Pseudo Genes (internal stop)      :: 45 of 223
            Pseudo Genes (multiple problems)  :: 57 of 223
            Pseudo Genes (short protein)      :: 3 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101202.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-151"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z151-24K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-15"
     gene            complement(29..562)
                     /locus_tag="NML27_RS26105"
                     /old_locus_tag="NML27_26100"
     CDS             complement(29..562)
                     /locus_tag="NML27_RS26105"
                     /old_locus_tag="NML27_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025389.1"
                     /translation="MNIQEALNIFGLSGQLTEKDIKNAFKKAALKYHPDRNPVGAEMMK
                     AVNSAFDFLMANLDKINQYQSPEQSAIYNYGDELENVLKALYGLSGLVYEVIGNWIWIS
                     GNTIEHKEALKEIGCKWAAKKKQWFYRPEEHKSYWNREEHTIEEIRAKYGTSGKFQAGG
                     WQQVEGRARVEARA"
                     /name="J domain-containing protein"
     gene            complement(933..1232)
                     /locus_tag="NML27_RS26110"
                     /old_locus_tag="NML27_26105"
     CDS             complement(933..1232)
                     /locus_tag="NML27_RS26110"
                     /old_locus_tag="NML27_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001615573.1"
                     /translation="MARLPYIESTTQQIFIFNTLTLCTIGLIHIETGKKYIVVFTDNNK
                     IRDYKNGVIEELGEITQEDIELIEYYAKKYEKYFDDLKIGEEPLNLKEYIECMQ"
                     /name="hypothetical protein"
     gene            complement(1306..1578)
                     /locus_tag="NML27_RS26115"
                     /old_locus_tag="NML27_26110"
     CDS             complement(1306..1578)
                     /locus_tag="NML27_RS26115"
                     /old_locus_tag="NML27_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283543.1"
                     /translation="MSAFIFRTTFKATGDQQKDAITQIKVMQEQAVKAFVSWMTNQEGT
                     EFLSTLQEITTELDALIDAVESHSPVFRKKATELEALLAHYKTLL"
                     /name="hypothetical protein"
     gene            complement(2537..3172)
                     /locus_tag="NML27_RS26120"
                     /old_locus_tag="NML27_26115"
     CDS             complement(2537..3172)
                     /locus_tag="NML27_RS26120"
                     /old_locus_tag="NML27_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371526.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="WP_000088100.1"
                     /translation="MSRKRRRFLNRWIKSLTTVAGRIKIKNELRRRIRHNRYWVNEANK
                     FGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGHKSISRASRGCDRLV
                     WLNAIITEKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERARLLKADPDEVIYS
                     GDARLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHSPTPIPA"
                     /name="RepA family replication protein"
     gene            complement(3821..4189)
                     /locus_tag="NML27_RS26125"
                     /old_locus_tag="NML27_26120"
     CDS             complement(3821..4189)
                     /locus_tag="NML27_RS26125"
                     /old_locus_tag="NML27_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248966.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_029397050.1"
                     /translation="MVPKRLKEAREAAGLSQEKLSELVGIEGTSTRSRMSNYEAGRFTP
                     PFKFICRVADVLGYPEYYFYIVNDVTAKKLLQMYKGEKNSIENIELEASRMADQLDDAR
                     KLVSQLSDCLRELKRSLG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            4443..4892
                     /locus_tag="NML27_RS26130"
                     /old_locus_tag="NML27_26125"
     CDS             4443..4892
                     /locus_tag="NML27_RS26130"
                     /old_locus_tag="NML27_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998906.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_249540331.1"
                     /translation="MNAWYLLYCNNNELDRIAYRCKTMNVVAFCPRYIKITPRKDCNAV
                     RKEEKVLFPNYLFLHFDVNVTHTSAITSIPGAIGFVSFGSTICTVPEKVVIAIKCAKIL
                     ALNPDDDSIECRNIGSDLLAEIQRISSIISPLERQTAFSRLLQNQ"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            4969..5181
                     /locus_tag="NML27_RS26135"
                     /old_locus_tag="NML27_26130"
     CDS             4969..5181
                     /locus_tag="NML27_RS26135"
                     /old_locus_tag="NML27_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001133545.1"
                     /translation="MPNPIGQAKSTAVIYIGKKRYETLAKHARDISYVAEVNIRPSAFL
                     QYMIDKLSNEAHNEMLKELMSKEDK"
                     /name="hypothetical protein"
     gene            5182..5388
                     /locus_tag="NML27_RS26140"
                     /old_locus_tag="NML27_26135"
     CDS             5182..5388
                     /locus_tag="NML27_RS26140"
                     /old_locus_tag="NML27_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029397048.1"
                     /translation="MPKPTYNVYVTQEVPTDENGGKNTYWTKVGAAFAHNGKPGLNIML
                     VPGIAVSGKLVLLEPKEDTPQGN"
                     /name="hypothetical protein"
     gene            5404..6108
                     /locus_tag="NML27_RS26145"
                     /old_locus_tag="NML27_26140"
     CDS             5404..6108
                     /locus_tag="NML27_RS26145"
                     /old_locus_tag="NML27_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_094283541.1"
                     /translation="MLSTVSFMAVAMQCAATVHPSTSLDVARVESGFNPYAIAEIIPKR
                     ERQPGDKGFISHMPKTKEDALSIVKQIEAKGRRYSVGLMQITSTNFNSYAVTAADLFNP
                     CTNLSVFEKIITDCYQRGGTLKRALSCYYSGNFTTGQQPEAALSRTSYIQRIGYSPEKP
                     RYVVPGTKDDIATQSAILNATPVEAPARPRVVWPGAIVRGVPAQLRQKKADTVYYPAQV
                     IRGNRFSTEKED"
                     /name="lytic transglycosylase domain-containing protein"
     gene            6112..6408
                     /locus_tag="NML27_RS26150"
                     /old_locus_tag="NML27_26145"
     CDS             6112..6408
                     /locus_tag="NML27_RS26150"
                     /old_locus_tag="NML27_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_029397047.1"
                     /translation="MFNKLKAICSRASAYILAPLVSVVSPVFAADGFSRANTIMEKVSN
                     GLHGLAAVTITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
                     /name="TrbC/VirB2 family protein"
     gene            6421..9165
                     /locus_tag="NML27_RS26155"
                     /old_locus_tag="NML27_26150"
     CDS             6421..9165
                     /locus_tag="NML27_RS26155"
                     /old_locus_tag="NML27_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_001096401.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLTVVGVTIIVAIWTSIALLLFLP
                     IEFLVMKSLTRNEPLRFNLIAVWLRVRGNPVANRLFGATAFMPVEHNAVDIQEFIDAMK
                     LNQCATIKKYIPYSSHIHPNVVRSLNSDFFCTWELTGTPFDCESDETLQFGTNQLHGLI
                     RSFEGFPVTFYVHNSREQFTDSLHKVSGNPYADTVSERYYASLNKRPFRRNRLFVTICY
                     RPFASLDKANRKRMKDGQKLKEIDSALLEVLEIKSTLDTALSRYGAHALGTFTENEAVF
                     SSQLAFYEYLLTHQWRKVRVTRTPAYNVMGNVALYFSAESGQINHVNGTQYFRGLEVKE
                     FSEETATGMTDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLMMSADDDAVSQRLDL
                     DVALDLLTSGKIAYGKHHLSIMVFSPSLESLIADTNEISNALNNIGITPVPAEISLSAA
                     YMAQLPGNYNLRPRAGELSSLNFVELASLHNFYPGKRDKAPWGDAMALVRTPSGDGYYL
                     NLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLQINQQKYNRDDSFSPDARTKRL
                     TTVYLDKDRGAEINIRALGGKYYRVFSGEPTGWNPFALPATKRNINFIKQLMKILCTRN
                     GATITPRDERRLNDAVNAVMSDEPQYRVYGITRMLENLPEPATREAQENGLSIRLSQWA
                     RGGEFGWVFDNEHDTFDISNCDNFGIDGTEFLDDPNVCAPISFYLLYRITSLLDGRRLV
                     IFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDEIAPAVIEQCGTQ
                     ILAANPSADRSHYVDGMKFEPEVFDVIKGLDPQARQYVVVKNQFRRGDTKRFAARITLD
                     LSGIGRYTKVMSGDAPNLEIFESLYQEGMKPSDWLDSYLAKAL"
                     /name="VirB3 family type IV secretion system protein"
     gene            9180..9884
                     /locus_tag="NML27_RS26160"
                     /old_locus_tag="NML27_26155"
     CDS             9180..9884
                     /locus_tag="NML27_RS26160"
                     /old_locus_tag="NML27_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_032173316.1"
                     /translation="MKRFRPLILAVLIASPIVNAAGIPVFDATARLENIRQWTKEANQW
                     LQTVQHYKDQLQAYQKQLETVTGIRDIQAFVNQAKSLKADLDKLRKPGQALNDLLLSGG
                     SSGQFDELYNKYKIFDNCNAEQSKSYANTCRQEVINKAIQIEQTNEVQTQIGEMLGEIN
                     ELSDRVANAKDMKEAQDLANSIQLKSVMLNTLTTQWEMNVKAAEKREKMLAEERRKKWN
                     QQQLNAPEIMFN"
                     /name="type IV secretion system protein"
     gene            9896..10123
                     /locus_tag="NML27_RS26165"
                     /old_locus_tag="NML27_26160"
     CDS             9896..10123
                     /locus_tag="NML27_RS26165"
                     /old_locus_tag="NML27_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000720509.1"
                     /translation="MKKIILFTIISGLMALTSGCKEEAKTTKWYREHPDELKVVYAECQ
                     KTGSASENCKHANEAHWQIQQLNAPEISFN"
                     /name="EexN family lipoprotein"
     gene            10133..11203
                     /locus_tag="NML27_RS26170"
                     /old_locus_tag="NML27_26165"
     CDS             10133..11203
                     /locus_tag="NML27_RS26170"
                     /old_locus_tag="NML27_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_024187435.1"
                     /translation="MMAGIFSSVEKNIIGGITDATNGLMMSYSSMMMGLAATSATIYIM
                     WRGYQTLAGKLSTPMEDVMWDIMRMAIILSFVANINGYLDGVIDAVNGIKEGFSSSTSI
                     WQLLDTLWNSARVLGKTLHDLDNSEFIKEEGTIAELFVWGGVFVLMIITAFVSMIAEIM
                     ILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTIMFSALALRIVIGFLTSRMSEA
                     VTQASKANIVELGAQVCLAGVCAAILVYMSAKLAGALAGASATATMQGAAALGIGAAAY
                     GAGKMLKEAGKVLPKEVASPSNKGLGDPQSRIVFNAADARKHAIEQMIARNEARMLTDQ
                     SAPKLPPSIKRLPPPR"
                     /name="type IV secretion system protein"
     gene            11251..11394
                     /locus_tag="NML27_RS26175"
                     /old_locus_tag="NML27_26170"
     CDS             11251..11394
                     /locus_tag="NML27_RS26175"
                     /old_locus_tag="NML27_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000825481.1"
                     /translation="MKRLIALALLCVLSGCAQKSKSLPPAFSGQGSPVNTPQIIAELTT
                     HD"
                     /name="hypothetical protein"
     gene            11387..12070
                     /locus_tag="NML27_RS26180"
                     /old_locus_tag="NML27_26175"
     CDS             11387..12070
                     /locus_tag="NML27_RS26180"
                     /old_locus_tag="NML27_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000139289.1"
                     /translation="MTEQKLIADSRSFEQQMIDRDKRATKAGFLVGGAGLLIAILSLIA
                     IVIMLPLKQTTVELYTVDNHTGRVEHVTRASKTALTAEEAYQKAMAANYVKLRERYVYP
                     ALQDDYETVQIYNSPQVNDDYLALYAGKNAPDKVYNNAAHTVKIEIISEQITNATAPDK
                     VATVRYKKIIRRMADNSIRNEYWDARFTFHSDPEKEMSDVEREINPFGFTVTSWQTDRE
                     IRGGE"
                     /name="type IV secretion system protein"
     gene            12070..12975
                     /gene="virB9"
                     /locus_tag="NML27_RS26185"
                     /old_locus_tag="NML27_26180"
     CDS             12070..12975
                     /gene="virB9"
                     /locus_tag="NML27_RS26185"
                     /old_locus_tag="NML27_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_094283540.1"
                     /translation="MRKLTLITLIIMLNSQAWAAATPQASRYDARIQQVIYNPQNVTVV
                     NTKPGFMTTLVFDNDEAVISASPGFDEAWEARADANRVNIRPVALTQGAPGEDGNTTQV
                     VIPPNSRDWHTNMLIVTNKRLYNVELNVIDEKSPQQPAYLVSYRYPGDERNRANKEAAA
                     RQKEWEQKQEQAQIQKALRTAQTPRNWDYTKYPGKDSYSIEPDFAYDDGRFTFIGFSPN
                     KSVPSVTKEQNGQEYVVNSSIQRKGNFTVLVIQEITPRLVLRSGDAVVGLKNNGFGKVH
                     ATDNSTVSDKVERVEKTDNI"
                     /name="virB9"
     gene            13017..14267
                     /locus_tag="NML27_RS26190"
                     /old_locus_tag="NML27_26185"
     CDS             13017..14267
                     /locus_tag="NML27_RS26190"
                     /old_locus_tag="NML27_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="WP_146999495.1"
                     /translation="MTDENKSPSTEEEKPVTTPDQDDIATLEREARAKREAELQALQDD
                     EKENSLQPAVNKLKKRPKGKATAFLAICAVALIGLTWGGNWVYQNFLTQEDGEKTKEVT
                     PPKKTNDYRKRTDLGMATNEPENSTEGEQTVNQDNTGNVAGTAGQMKTFEPPQLNKASF
                     LVRRNNTASPQNQVKTRQQEMILTAENNATQPTDSTATTTQHGTASQAISPATVRRIPY
                     NPDLYIPENTVIPCSMDYRFVSDRAGRIRCTVAKDIWSASGNTKLIEKGTTATGIYQTG
                     ITQGQGRAFIMMTKLRTRQRPYLDIPLIDTNAAGELGESGVDGWIDTHFWERFGGALMV
                     GMIPDIGAWASNNAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGEIINL
                     ITGQDIDFSNIYTLRMK"
                     /name="TrbI/VirB10 family protein"
     gene            14271..15296
                     /gene="virB11"
                     /locus_tag="NML27_RS26195"
                     /old_locus_tag="NML27_26190"
     CDS             14271..15296
                     /gene="virB11"
                     /locus_tag="NML27_RS26195"
                     /old_locus_tag="NML27_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125556.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_044862971.1"
                     /translation="MTVKNISLDRYKQRFFSEYLELPGLTEIAVNRPGELWTKIDGVWG
                     QHTVDLKFDDCYNFARCLAKHHNDNIEDIKPGLSATLESGERCQVVVPPACERDTISIT
                     IRKPSKIQIPHQAYIDSGFYNRVTGIEKASTHNEELIELYKSKNIPLFMEKCVEYGKTL
                     AVAGETSTGKTTYMKMLIQYIPVHLRITTIEDNPEIIFYKHKNYVHLFYPSESSDEKGS
                     VVTPAALIRWNYRMNPDRILLTEVRGGEAWDFLKITGSGHEGSMTSIHAGSAHEAIDGF
                     ITRCTENPQCAQLPYSFLLRKVLDSLDVVVSIDLDGNIRRMNDIYFRPLHRNQYYEEMK
                     V"
                     /name="virB11"
     gene            15293..15691
                     /locus_tag="NML27_RS26200"
                     /old_locus_tag="NML27_26195"
     CDS             15293..15691
                     /locus_tag="NML27_RS26200"
                     /old_locus_tag="NML27_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_094283556.1"
                     /translation="MIKHILVTVFVTLITGCSSPPPPVPVEWNKKPVSLNDKLPEWKPN
                     SLIIKSPVINDKWTLSISSQDFNKEDWMPSVFYAIAHSTQITVATKSGADYFSTKNWLY
                     HHGANGVIIYHPIINCLTCNETTIYFSR"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            15721..16020
                     /locus_tag="NML27_RS26205"
                     /old_locus_tag="NML27_26200"
     CDS             15721..16020
                     /locus_tag="NML27_RS26205"
                     /old_locus_tag="NML27_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000716758.1"
                     /translation="MKKIAAITMFVLTTATPVYSYAADPCEVVLCMYGKATGNSGGGEC
                     RSAEKAFFNILKKKKGSIRWSKTFDARKAFLNQCSSADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            16023..16238
                     /locus_tag="NML27_RS26210"
                     /old_locus_tag="NML27_26205"
     CDS             16023..16238
                     /locus_tag="NML27_RS26210"
                     /old_locus_tag="NML27_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283555.1"
                     /translation="MNTDENMVFYLTNSDLVYSYAHKIVHSIPSGMKGKFITSIGETIE
                     DLKKRHPDIILITESEALALQSERKN"
                     /name="hypothetical protein"
     gene            16266..16571
                     /locus_tag="NML27_RS26215"
                     /old_locus_tag="NML27_26210"
     CDS             16266..16571
                     /locus_tag="NML27_RS26215"
                     /old_locus_tag="NML27_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="WP_094283554.1"
                     /translation="MLKEDMFINNIHSRNQDRIHVKRVFDDISNKANRGCGLYYEIYQA
                     RLMRGLIEHTVKLKSSDANKLISYAASQGYEITEDRYNAVIKAEDECLTEITRAQV"
                     /name="dpoa decarboxylase"
     gene            17381..19108
                     /locus_tag="NML27_RS26220"
                     /old_locus_tag="NML27_26215"
     CDS             17381..19108
                     /locus_tag="NML27_RS26220"
                     /old_locus_tag="NML27_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615560.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_094283553.1"
                     /translation="MNPHLMQKLNGIADAFSIFLLWLQNSPVILSLLAGLTLPFIFNLP
                     REERKNAPFWLKSVACVSIFFFIFGTMSPLSIHGLSYLFDVLDPNTLLRIPLWIITILF
                     TLAGLAFHLTARRLLAGEIDSLKHRMIKKSKLERNTRTDVRKVKELLPDSIEYNPLDYI
                     DLKKGVFIGLDKDDQPQYITIKEFQKQHAAIIGTTGSGKGITASVLLYQAILLGEAVFV
                     EDPKDDEWAVHVLREACKKAGKKFVVINLNRTNHQLDLLADISHEQLEELFNAGFSLSK
                     KGEAADFYRIADRRAARNVAAIHKTGMTLYDLFNDDNVKSIGEDAPAFLGELEEIALVN
                     SVNAKKGFSLKEIFDNGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINTTPRVV
                     AIFLDELKYHISRPTMEGLGTARDKSVHIFMAFQAIDDLRDCPSDLNGDSVIGAVIENA
                     KFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDS
                     NMFLNLPERVGFVFTSKDLPKATKMSHIPANKETIELLSFETEQIEQHNNNDVPSSGL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            19118..19852
                     /gene="mobC"
                     /locus_tag="NML27_RS26225"
                     /old_locus_tag="NML27_26220"
     CDS             19118..19852
                     /gene="mobC"
                     /locus_tag="NML27_RS26225"
                     /old_locus_tag="NML27_26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094283552.1"
                     /translation="MLIHDFYERKRISANNMLCLLNFLKDETYSDFHIIKRKLGFTKKS
                     RNPVYKLLNKALKEGFIIKHEIPSSKRKLTIWGITMQGLAMVVKADDITFPSYFEPSKL
                     KYWTLEHRLLNQRVRISLESKGATEWINGDSAAFIARYSGCSHRPDGVIKQPTGAIMAI
                     ETERTMKTRARYISIINSHLTARDKGNWHYALYVMPDTKSKESLIRLFDSIKTVMRNNT
                     PVPFDEKNREMFIFRTIDELEQ"
                     /name="mobC"
     gene            19856..20335
                     /locus_tag="NML27_RS26230"
                     /old_locus_tag="NML27_26225"
     CDS             19856..20335
                     /locus_tag="NML27_RS26230"
                     /old_locus_tag="NML27_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_094283551.1"
                     /translation="MKILIIFALYILSPSLYANAADIRGKVIRVLDGDTIEILQNKKPV
                     RIRLANIDAPEKKQAYGRWSTDQLKSLIAGQSVRVTYSHNDRYGRIIGRVFTTGGSEAN
                     RFMVQSGAAWVYEHYNTDNALADLQQEARSQKRGLWADPNPVPPWEWRHKYKVTK"
                     /name="thermonuclease family protein"
     gene            20332..20709
                     /locus_tag="NML27_RS26235"
                     /old_locus_tag="NML27_26230"
     CDS             20332..20709
                     /locus_tag="NML27_RS26235"
                     /old_locus_tag="NML27_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283550.1"
                     /translation="MNDCFGCMHPYPAGHWFYSLQDLLTPLLENPLFYVFSFFFAGMIG
                     AIISRITKLIKTMKHSNNNNNKISIDNYDQIEIERYMTNEEVGRLVINFMKRTNGEPLL
                     IVFPEYDNGDDNDDKNTRCIK"
                     /name="hypothetical protein"
     gene            20678..20941
                     /locus_tag="NML27_RS26240"
                     /old_locus_tag="NML27_26235"
     CDS             20678..20941
                     /locus_tag="NML27_RS26240"
                     /old_locus_tag="NML27_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_094283549.1"
                     /translation="MTIKILVVSNDGHEKLIDLSPVNDLAKITKSLRTSENRMVCVIQD
                     NNRILRWDRNYASRAKNHWRKVAPDRFEILGTIEHIHYVGKC"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(20973..21206)
                     /locus_tag="NML27_RS26245"
                     /old_locus_tag="NML27_26240"
     CDS             complement(20973..21206)
                     /locus_tag="NML27_RS26245"
                     /old_locus_tag="NML27_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000024515.1"
                     /translation="MANVPPKPRNTKGEPPSLEDTQHGFNPTRKNADGRKAQLNLYVDM
                     EVKREFRSIAAANDMKHGELLEAYQEFWKKHH"
                     /name="hypothetical protein"
     gene            complement(21246..21944)
                     /locus_tag="NML27_RS26250"
                     /old_locus_tag="NML27_26245"
     CDS             complement(21246..21944)
                     /locus_tag="NML27_RS26250"
                     /old_locus_tag="NML27_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214917.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_249540332.1"
                     /translation="MDTIIIFFYKNIFIGGRMTVAIALVNGKGGVKKSSMSRAIATEYT
                     KADWKVLGLDMDVDQGSFLDWGQRRLRYKVEPPVTIQGCGTVSQAKKHIESGIWDLVVI
                     DSAAYATKSLLSLAEIVDLLVLPSGFSVDDLRTTVTTVNALRNKGHSTNKMAVVFVGVS
                     ESESEYRTALEYLKPCGVQVINGAIPFMTSFSQAQDKGLALTESPFPGPRQKADEVVQG
                     VIDLLTKLSQ"
                     /name="ParA family protein"
     gene            22256..22606
                     /locus_tag="NML27_RS26255"
                     /old_locus_tag="NML27_26250"
     CDS             22256..22606
                     /locus_tag="NML27_RS26255"
                     /old_locus_tag="NML27_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006705960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094283547.1"
                     /translation="MWNVVTTDIFDAWFLTQNEDLRESVYEAMGLLEKFGPTLGRPYVD
                     TLNGSDFANMKELRVQYKGSPVRAFFAFDPTRNAIVLCAGDKTGLNEKRFYKDMIKLAD
                     SEYRKHLAKLEK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            22608..22907
                     /locus_tag="NML27_RS26260"
                     /old_locus_tag="NML27_26255"
     CDS             22608..22907
                     /locus_tag="NML27_RS26260"
                     /old_locus_tag="NML27_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806992.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_094283546.1"
                     /translation="MGTYRELLAKESPEMQARVAERVEKASVQIALSALRDELNISQTE
                     LATMMGVKQPSVARMEQTDNDPRISTLKRYVKALGGELSLDVTLPTGKRVAFHL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            22914..>23024
                     /locus_tag="NML27_RS26265"
                     /old_locus_tag="NML27_26260"
                     /pseudo=""
     CDS             22914..>23024
                     /locus_tag="NML27_RS26265"
                     /old_locus_tag="NML27_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /name="transcriptional regulator"
     gene            complement(23099..23338)
                     /locus_tag="NML27_RS26270"
                     /old_locus_tag="NML27_26265"
     CDS             complement(23099..23338)
                     /locus_tag="NML27_RS26270"
                     /old_locus_tag="NML27_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283545.1"
                     /translation="MQTKPTPADVKEARVSAGLTLKEAAEIFGYQLNTWQMKESAGKAT
                     RSLSVGEYNYLLLLAGKHPEYVLCKRDTVGTLNN"
                     /name="hypothetical protein"
     gene            complement(23328..23585)
                     /locus_tag="NML27_RS26275"
                     /old_locus_tag="NML27_26270"
     CDS             complement(23328..23585)
                     /locus_tag="NML27_RS26275"
                     /old_locus_tag="NML27_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024192529.1"
                     /translation="MKTDNVFFKSKGSYSYVVTVDMDNQVYMVRKYVKTYVKSCDKEQV
                     KGKKTVKKLVLDTNLTLPELLSFDNGILADLFTRGNNNAN"
                     /name="hypothetical protein"
     gene            complement(23622..24164)
                     /locus_tag="NML27_RS26280"
                     /old_locus_tag="NML27_26275"
     CDS             complement(23622..24164)
                     /locus_tag="NML27_RS26280"
                     /old_locus_tag="NML27_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283544.1"
                     /translation="MTTFSEKYQPVFEVVCRILGNGWRVNKLDNYQHRIKLTSTEIRKY
                     SIIIRLERERFFIVGSVDSLVGHGNPSVCTVSLTRNPVGIANDISRKILANALDEIELL
                     REHESALQEKQDKVRIIKGMLSQLFSISPWYGALTGFNADNGLAGKILQNGDDYEMVIR
                     GLNVDQLIKVAGFIKKL"
                     /name="hypothetical protein"
     Typing          19119..19849
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.361"
                     /coverage="100.0"
                     /evalue="3.71e-162"
     Typing          6422..9162
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="87.527"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11388..12067
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="85.903"
                     /coverage="100.0"
                     /evalue="3.85e-119"
ORIGIN
        1 gatatagggg gcttacgccc ccgtttcgtt atgctctggc ttcaactctt gccctgcctt
       61 caacttgttg ccatcctccg gcctggaatt ttccgcttgt gccatatttt gcgcggattt
      121 cctctatcgt gtgctcttca cggttccagt aacttttatg ttcttctgga cgataaaacc
      181 attgtttttt ctttgccgcc catttacacc ctatttcttt taatgcttct ttgtgctcga
      241 tagtatttcc actaatccag atccagttac caataacctc ataaactaaa ccagacaaac
      301 cgtaaagagc ctttaaaaca ttttctagtt catcaccata gttatatatt gcactttgtt
      361 caggactttg gtactgatta atcttatcaa gattcgccat taaaaaatcg aatgctgaat
      421 taaccgcctt catcatttca gcacctaccg gattacggtc tgggtgatat tttaatgcag
      481 ctttcttaaa ggcgtttttg atgtcttttt cagtcaactg gccagacaag ccaaaaatat
      541 ttaatgcttc ttggatattc attttgtagc tcccgcccct gtggggctaa gatgagaggg
      601 aaaccatttc cctctcgatg atttaattat accttcaatt gcaggtattg caagtttttt
      661 agctctcttt ttgaaaaaat ttataattac tgtcaggaca tcaacattgc ttaaaacatt
      721 tcatatccct gtaaataatt taaccaccac ctgaattgat cccaatccgt aaatttcatt
      781 gctgggattc tcgtttccca tcctttgaca agtgaatccg caagttgtac gatctgacct
      841 gttgacattt gaacacctct ctattgtttt gagtgtcgat aaagtgatca ggttgctcga
      901 ccaacctgat cacaccttta tagaatatat ggctattgca tacattctat atattcttta
      961 agattcaaag gctcctcacc tatttttaaa tcatcaaaat acttttcata ttttttagca
     1021 taatactcaa tcagttcaat atcttcttgc gtaatttccc ctaattcttc tattacacca
     1081 tttttataat ctctaatctt attgttatct gtaaatacta caatatattt tttacctgtc
     1141 tctatatgaa ttaatccaat agtacaaagt gtcaaagtat taaaaataaa aatctgttgc
     1201 gtggttgatt caatataagg taagcgagcc atttttaaac tccacttgct ttagatttgg
     1261 attaaataaa gagccatata acggggcaat tgccccgttg aggacttaca gcaacgtttt
     1321 gtaatgggct aaaagcgctt ccagttcagt cgctttcttt ctgaaaacag gggaatggga
     1381 ctctaccgca tcaatcagtg cgtcaagttc tgttgtgatt tcctgcaaag tgcttagaaa
     1441 ttctgtaccc tcttggttgg tcatccagga aacaaaggct tttactgctt gttcctgcat
     1501 aactttaatt tgggtaatag cgtctttttg ctgatcgcca gttgctttaa atgtggttct
     1561 aaaaataaat gcactcattt taatctcccg cccctatggg gctaagtgga aagggaacct
     1621 atttcccttt cgatgatttt attatacctg catatgcagg tattgcaact atttttcact
     1681 attcgaactg aaaaattttc caccactcac ctttaattcc ttcgcgtatg tgctcaattc
     1741 agcaagggca ctattccgct tcctcaccca ctcgctcact aacgttcggt cgctagggtt
     1801 gcggcgagcg gatctacttt cctagggttg tacaccggaa acgccgaaat ttccgatttc
     1861 caaactgacc tccaacagga acctttttgt gatctttctc gatcatattg ttagcagtta
     1921 gttaagttat gctcgatggt gggaagatgc cgcgttagcg gcatgtaagg cgcgaaatac
     1981 agttccatag aatcacaaat gctaaggcgc gccgataccc ctaatcaagg acgttcatgt
     2041 cgccaggaac cgcgtcagcc ccctgctaag ccaaaattgc acggaactta cagctactta
     2101 ggtggtaggt ggtcgtagac cgtcgatgtt acagcgatga taggtgaatg attgttcgtt
     2161 tttaatgacc ggatatggtg tctctaatgc cccgatgctt gcgaggggtg ctacctttga
     2221 ggcgcttgcg ccgatgcttt tcagttatcc acttgtaaaa ggggataaca gtaatgtttt
     2281 tctttttgct tctcttttag tacgtaagta actaaaagag aagcaaaaga gtaattgcgt
     2341 catatcgttt gcacttcact tatgattatc cacaagctgt acatcgttta ccctctggcc
     2401 ataacctatg gctgctcttt cctaagcagg gagccgcttg gaaaacggaa aatatctcac
     2461 gctaagacta ttttttgttc agagatttat tatggacgtg acgatatacc gcccataagg
     2521 cggtaagtag ccagcatcag gccggaatgg gggtaggtga atgtgcgtaa tattcttttt
     2581 tgcgttgtcg agctgcgtcg cgttttgctt tcatcctggc taatccggct gccgctttcc
     2641 gcgtccagtt ctggactctg atagctataa gtctggcatc accggagtaa atcacttcat
     2701 caggatctgc ctttaacagc ctggctcgtt cccgataaac cagttttaac ggaatgccaa
     2761 ggatggcaaa gaaatcctcg gttacttcaa tatgtttaca ggcacaccgt gcatcatacg
     2821 ggttaaacgg agccttctct gtaatgatgg cattcaacca aaccaggcga tcacaaccac
     2881 gactggcgcg agaaatggat ttatgtcccg catcgctgcg tgtaaccagt ccggcacgtt
     2941 cggcaagcgt ttcaaggtta tgcctggttt cccacgagcg taaatcgaga tcgtccagaa
     3001 tagctaacat caattcgcaa agagcctcta tgccaaactt gttagcctca ttaacccaat
     3061 agcggttatg ccggatacgg cggcggagtt cgtttttgat tttgatacgc cctgcgaccg
     3121 tcgtcagcga tttaatccag cgattgagga aacggcgtcg cttacgcgac acaacgttat
     3181 agcatggact ttgttttgcc acccctgacg gcgatatgcg ggggattaag gagttgttct
     3241 tttcagccac caccgcccgc acttattgcc ggacagaggt cttgtattga gaaatgcatt
     3301 tgctataatc ctttgcaggc acccagcctg ctttggaccc cctgattatc ttcttggacc
     3361 ggcaaaatcg aagagagata tcagggggtt gttgcattca agggggggct aaaatgcttc
     3421 cccccccttc ccttggacgc tgttacggga acgtcccacc caaaaatccg taaaaaccgc
     3481 acgaaaatag catgaaccca ttcgaagatc aattcatcca caaaagatcc cgccgattac
     3541 cttcctattt ttctttaaac ttcatctggt tatataattc ttaacaaaat tgagattaaa
     3601 taccgtacag catataactc acgatattaa tgctaatgta cgcatacatt agcattaata
     3661 tcgaaaagaa atcggcgata acaaaccgaa aaataacgga atttaatagg gtatttaccg
     3721 ctcgccacaa ccctagcgac cgaacgttag tgagcgagtg ggtgaggaag cggaaaagcg
     3781 acttgaccaa atggagcaca tacgcactga tgcccgaaac ttaccctaac gaacgtttaa
     3841 gctctctcaa acagtcgcta agttgagata ctagttttct agcatcatca agttgatcag
     3901 ccattctact tgcctccaac tcaatgtttt caatgctgtt tttttctccc ttgtacatct
     3961 gaagtaactt tttagcagtt acatcattca ctatatagaa ataatattct ggatatccta
     4021 agacatcggc cacccgacag ataaatttaa atggtggcgt aaaccgtccc gcctcataat
     4081 tagacatcct agatcttgtg ctagtccctt ctatccccac taactctgat agcttttcct
     4141 gagataaacc agcagcttcc cgcgcttctt tcaatcgttt tggcaccatt gacccgatcc
     4201 ccttgttttg ttttccaaaa catacatcgg caccaatttt tgaatgttca tacatccaaa
     4261 acctttgact taatgaacac acttgttatt atttctttag attcttgata ttgacacgca
     4321 agaatagctg tataaaatca caactgtgta tttttacagt taacacttaa tgagtacgcc
     4381 gtcctaagcc cggcaactta ggacggcgga aaatgggggc ttgtgtccgt aaggtgacgc
     4441 ttatgaatgc atggtatctt ttatattgta ataacaacga gttagaccgc attgcatatc
     4501 gttgtaaaac tatgaacgtt gtggctttct gtccgagata cataaagatc acacctcgga
     4561 aagattgcaa cgcagtacgc aaggaagaaa aagttttatt tcctaactac ctcttcctcc
     4621 attttgatgt gaatgtcacg cacacatcag caataacctc tattccaggt gcaattggct
     4681 tcgtaagttt cggttccaca atatgtactg taccggaaaa agtcgttatc gctattaaat
     4741 gcgccaagat actggcgcta aatcctgatg atgattccat agagtgccgt aacatcggta
     4801 gtgacctact ggcagaaatt caacgcatat cttcaatcat ctcaccgtta gaacgtcaaa
     4861 cggccttctc caggctttta cagaatcagt aaaccagacc ttcattcctt ctccagtcgt
     4921 accgctgtgc ttttgcacac ggatatttca tacaggagct ataaactcat gccgaaccct
     4981 ataggccagg ctaaatctac agccgttatt tacatcggca aaaaacgcta tgaaacactg
     5041 gcaaaacacg ccagagatat aagttatgtt gcggaggtaa atattcgtcc aagtgccttt
     5101 ttgcagtaca tgatcgacaa attaagtaat gaagctcaca acgaaatgtt gaaagagcta
     5161 atgtcaaaag aggataaata aatgcccaaa cccacctata acgtatacgt cacacaggaa
     5221 gttcccactg acgaaaacgg cggtaaaaat acctactgga ccaaggttgg cgcggcattc
     5281 gcgcataacg gaaaaccagg actgaatatt atgctcgttc ctggtattgc tgtttccggc
     5341 aagctggtac tactcgaacc taaagaagac accccacaag gaaactaatt tagcgagggt
     5401 actatgcttt ccaccgtttc atttatggcg gtggctatgc agtgtgccgc cacagttcac
     5461 ccctccacat cactggatgt tgcgcgggtc gagtctggtt ttaatcccta cgctattgct
     5521 gagatcatcc ccaaacgtga acgccagcca ggcgacaagg gctttatctc acatatgcca
     5581 aaaactaagg aggatgcatt aagcatcgtc aaacagatag aggcaaaagg tcgccgctat
     5641 tctgttggtc tgatgcaaat cacaagtaca aattttaata gttacgccgt aacggctgct
     5701 gacctgttca acccgtgcac aaatctttct gtttttgaaa agatcatcac tgattgctac
     5761 cagcgaggcg gcacactgaa acgggcgctg agttgttatt actctggaaa ttttactaca
     5821 ggacaacaac cagaagctgc gttatccaga accagttaca tacagcgaat tggttactca
     5881 cctgaaaagc cccgctatgt cgttcctgga accaaggatg atatcgcaac tcaatcggcc
     5941 attctgaatg caacacccgt tgaagctccg gcacgccctc gcgttgtctg gcctggcgca
     6001 atcgtgcgcg gggttcctgc tcaactgcgc cagaaaaagg cagataccgt ttattacccc
     6061 gcacaagtta tacgcggaaa cagattttca actgaaaaag aggattaacc gatgtttaac
     6121 aaattaaagg ccatttgcag ccgcgcatct gcttatattc tggctccgtt agtaagtgtt
     6181 gtcagtccag ttttcgcggc tgacggtttc agccgagcaa ataccatcat ggaaaaggtt
     6241 tcgaatggtc tgcatggtct ggcagccgtc accatcaccg tcgctgtgat ctggattggc
     6301 tataagactc tgtggaaagg cgaaagcctg tcgcagtgcg gttacatcat cattggcggc
     6361 attttaattg gcggaggcag tgaaattggc gctctgctaa tgagctaaga ggccgaacat
     6421 atggcaaagc tgcttaaggc gatgaaacgt ccggcggcgc tgtggggggt gccaatggtg
     6481 cccctgctga ctgtcgtggg cgtgacaatt attgttgcta tctggacttc catagcccta
     6541 cttctcttcc tccctattga gtttctggtt atgaaatcac taaccagaaa tgaacctctg
     6601 agatttaacc tgatagctgt ctggctaagg gtaaggggta atccggtagc aaaccgttta
     6661 tttggcgcaa ccgcatttat gcctgttgaa cataatgctg ttgatatcca ggagtttatc
     6721 gatgcgatga agctaaacca atgtgccacc ataaaaaaat acattcctta ctcgtcacat
     6781 attcacccta atgttgtccg ttcactgaat agcgattttt tctgtacctg ggagctgaca
     6841 ggaacgccgt ttgattgtga atctgacgag acactacagt tcggtacaaa ccagttgcac
     6901 gggctaatac gctcatttga aggctttccg gttacatttt atgtacataa cagccgtgaa
     6961 cagtttacgg actcactgca taaggtttca ggaaaccctt acgcggatac cgtgagcgaa
     7021 cgctattacg catcactgaa taaaaggcca ttccgacgta accgcctgtt tgtgaccatc
     7081 tgctatcgtc catttgccag tctggataag gcaaaccgta aacgtatgaa agacggtcag
     7141 aagctaaaag agatagacag cgcattactt gaagtgctgg aaatcaaaag tacgctggat
     7201 acagccttat cccgttatgg tgctcacgcg ctcggtactt tcaccgaaaa cgaggctgta
     7261 ttctcgtccc aactggcatt ttatgaatac cttctcacgc atcaatggcg aaaagtcaga
     7321 gttacacgta cccccgctta caacgtaatg ggaaatgttg cgctgtactt ctccgccgaa
     7381 tcagggcaaa taaatcatgt taatggcact caatatttcc gtggtctgga ggttaaagag
     7441 ttttcggaag aaaccgcaac gggcatgaca gactccttac tgtacgcgcc gtgcgattat
     7501 gtgattacac aatcctatac ctgcatgtcc cgtgaggaag cgaaaaaggc gattaagcgc
     7561 acccgacggc tgatgatgag tgctgatgat gatgctgtat ctcaacgttt ggatctcgat
     7621 gtggcgcttg acctgctcac ctccggtaaa atcgcctacg gtaaacatca tttatcaatt
     7681 atggtgtttt cgccgtcact ggaaagcctg attgcagaca caaacgagat cagcaatgca
     7741 cttaacaata tcgggataac ccctgttcct gctgaaatct cattgtctgc ggcctatatg
     7801 gctcaattac caggcaatta caatctgcgg ccacgagccg gagagctgag tagcctgaac
     7861 tttgttgaac tggcatcact tcataatttc tatccaggaa aacgcgacaa agccccgtgg
     7921 ggtgacgcga tggcactggt gcgaaccccg tcaggtgatg gctactatct gaatctgcat
     7981 aacactctgg cagataaaga cgagttcaac gaaaaaaacc ctgcaagtac ctgcattctg
     8041 ggtacaaacg gttcaggtaa aacaatgctg atgacgtttt tacaaatcaa tcagcaaaaa
     8101 tataaccgtg atgacagctt ttcaccggat gccaggacta aacgactaac cacagtttac
     8161 ctcgataaag accgtggtgc tgaaatcaat attcgcgcac ttggaggaaa gtactacagg
     8221 gtattcagcg gtgagccaac aggctggaac ccgtttgctc tgccagccac taagcgaaac
     8281 atcaatttca ttaagcaact gatgaaaatc ctttgcaccc gcaacggtgc aaccataacg
     8341 ccacgtgatg aacgtcgcct caatgatgct gtaaatgccg tcatgagtga tgaacctcaa
     8401 tatcgcgtct acggcataac ccgaatgctg gagaacctac cggaaccggc aacgcgtgaa
     8461 gcgcaagaaa acggcctgag tatccgtctt tcacagtggg cgcgcggtgg tgaatttggt
     8521 tgggtgtttg ataacgaaca cgacactttc gatatcagca actgcgataa tttcggaatt
     8581 gacggaactg aatttctgga tgacccgaat gtctgcgctc ctatctcgtt ttatctgctg
     8641 taccggataa ccagcctgct ggatggcaga cgacttgtta ttttcatgga tgaattctgg
     8701 aagtggttac gcgaccctgt atttaaagat tttgcgtata acaagctgaa aactatccga
     8761 aaactgaacg gtatgctggt tgttggcacg caatcgccag cggaaataat caaggatgag
     8821 atagctccgg cagttattga gcaatgtggt actcaaatac tggccgccaa cccaagcgca
     8881 gatcgctctc attacgtgga tggaatgaaa tttgaaccag aagtgtttga tgtcattaaa
     8941 ggacttgatc cacaggcgcg gcagtatgtc gttgtcaaaa atcagttccg ccgtggagat
     9001 acaaaacgct ttgcagcccg tatcacgctg gacctgtctg gtatcggcag atacacaaaa
     9061 gtaatgagcg gcgatgcacc caacctggaa atattcgaat cactctatca ggagggaatg
     9121 aagcccagcg actggctgga ttcctacctg gctaaagcac tataaccaag gagatatcga
     9181 tgaaaagatt tcgccccctt attctggcgg ttcttattgc cagcccgata gtaaacgctg
     9241 ctggtatacc cgtgtttgat gcaacagcca gactggagaa tattcgccag tggacaaaag
     9301 aagccaatca atggctacag acggtacaac actataaaga ccagctacag gcgtatcaaa
     9361 aacaactgga aacagttaca ggcatccgtg acattcaggc attcgttaat caggctaaaa
     9421 gccttaaagc tgacctggat aaactacgta agccagggca agccctgaac gatttgctct
     9481 tatccggcgg ttcttctggc caatttgatg aactgtacaa caaatacaag attttcgata
     9541 actgcaatgc tgaacaatca aaaagttatg ccaatacatg cagacaagag gtcatcaaca
     9601 aagcaataca aatcgaacaa accaatgaag tacaaaccca gattggtgag atgcttggtg
     9661 aaatcaacga attatcagac cgtgtagcaa atgccaaaga tatgaaagag gcccaagact
     9721 tagcaaacag tattcagtta aaatctgtga tgctaaatac gctcacgacc caatgggaaa
     9781 tgaacgtaaa agcggcggag aagcgtgaaa agatgcttgc tgaggaacgc cgtaagaaat
     9841 ggaatcagca gcaattaaac gcaccagaaa taatgtttaa ttagcaggtg acttaatgaa
     9901 gaaaatcatt ttatttacaa ttatttcagg cctgatggcg ttaacatcag gatgcaagga
     9961 agaagctaaa acaacaaaat ggtatcgtga acatcctgat gaactcaaag ttgtttatgc
    10021 tgaatgccag aaaacaggca gcgcatctga aaactgcaaa catgccaatg aagctcactg
    10081 gcaaattcaa caattaaatg cacctgaaat aagtttcaac taaagggcgt ttatcatggc
    10141 cggtatattt tcttctgtcg aaaaaaatat catcggtggg ataactgacg cgactaatgg
    10201 tttgatgatg tcttattcgt caatgatgat ggggctggcg gctacgtcag ccactatcta
    10261 cattatgtgg cgaggatatc aaaccttagc ggggaagctg tctacaccaa tggaagatgt
    10321 tatgtgggat ataatgagga tggctatcat tttatccttt gtagcaaaca ttaacggata
    10381 tcttgatggt gtcattgatg ctgtaaatgg aataaaagaa ggtttttcca gtagcacaag
    10441 catctggcaa ttacttgata ctctatggaa cagcgcaaga gttttaggta aaacgttaca
    10501 tgatctagac aactccgaat ttatcaaaga ggaaggcaca attgctgaat tatttgtatg
    10561 gggtggtgtc tttgtcttaa tgattatcac agcattcgta tctatgattg cggagatcat
    10621 gatcttattg ttaggtatta ccgcaccaat atttatattt tgcctgatgt atggcttttt
    10681 acggccaatg tttaataact ggcttcaaaa tatatttgct gcaatactaa caattatgtt
    10741 ttcagcctta gctctgcgaa ttgtaattgg ttttcttaca tccagaatgt cagaagcagt
    10801 gacccaggcc agcaaagcaa acattgtaga acttggtgct caagtttgtc ttgccggagt
    10861 atgcgctgca atactggtgt acatgtccgc aaaactggct ggggcacttg caggagcaag
    10921 tgctacagcc actatgcaag gtgcggcggc tctcggcatt ggcgctgctg catacggcgc
    10981 tggaaagatg ttaaaggagg caggaaaagt attacctaaa gaagtagcaa gcccaagtaa
    11041 taaagggtta ggagatccac aatcgcgcat agtatttaac gctgctgatg cacgtaaaca
    11101 cgctatagaa caaatgatag cgcgtaatga agcgcggatg ctgacagatc aatcagcacc
    11161 aaaactaccg ccatcaatta aacgtttacc ccctccaaga taactataaa aatataatta
    11221 tatttttata gaaacaaaaa ggtacataaa atgaaacgct taatcgcgct ggctttgctg
    11281 tgcgtgcttt ctggctgcgc acaaaaatct aaatctctgc cgcccgcatt cagcggtcag
    11341 ggtagccctg taaacacacc ccaaattatc gcggagctaa ccacacatga ctgaacagaa
    11401 attaattgct gattcccgct cgtttgagca gcaaatgatt gacagggata aacgcgccac
    11461 aaaggcaggc tttctggtcg gtggagcagg gttattaatc gctatcctgt cactgatcgc
    11521 gatagtgatt atgttgccac ttaaacaaac aacagttgaa ctctataccg tagataatca
    11581 taccggacga gtggaacacg ttacccgtgc atcaaaaaca gccctgacag cggaagaagc
    11641 ctaccaaaaa gctatggccg ccaattacgt taaattacgt gaacggtacg tttatcctgc
    11701 attacaggac gattacgaga cagtacagat ctacaactca ccacaggtaa acgacgacta
    11761 cctggcactg tacgccggaa aaaacgcacc agacaaagtt tataataacg cggctcacac
    11821 cgtgaaaatt gaaattattt ctgagcagat aacgaatgca acagcaccag acaaggtagc
    11881 caccgttcgt tataaaaaaa taattcgtcg catggctgat aacagtattc gcaatgaata
    11941 ctgggatgca cgtttcacct tccattcaga ccctgaaaaa gaaatgtcag atgttgagcg
    12001 ggaaataaac ccgttcggat tcaccgtaac aagctggcag actgaccgcg aaatcagggg
    12061 aggcgagtaa tgagaaaact gacgttaatt accctgataa taatgctgaa cagccaggca
    12121 tgggccgcag ctacgccaca ggcgagccgt tatgatgcac gtatccagca ggtcatttat
    12181 aacccgcaga atgttaccgt tgtaaacaca aaacctggat ttatgacgac tctggttttt
    12241 gataacgatg aagctgttat cagtgccagt cctggctttg atgaagcatg ggaagccaga
    12301 gcagatgcaa acagggttaa cattcgtccg gttgcgctca cacagggcgc acctggtgaa
    12361 gacggtaata caacacaggt tgttatcccg ccgaacagcc gtgactggca taccaatatg
    12421 ctgattgtga caaataagcg cctgtacaac gttgaactga atgtcattga tgaaaaatcg
    12481 ccacaacagc ccgcatacct ggtgagttac cgctatccag gggacgaacg taacagagcc
    12541 aataaggaag cagcagcacg tcagaaagaa tgggaacaaa agcaggaaca agcccagatt
    12601 cagaaagcac tgagaaccgc acaaacccca cgtaactggg actacacgaa atatccagga
    12661 aaagacagtt acagcattga acctgatttt gcctacgacg acggtcgctt tacgtttatc
    12721 ggattctcac ccaacaaatc agttccatca gttaccaaag aacagaacgg ccaagaatac
    12781 gtggttaaca gcagtataca gcgtaagggc aattttaccg ttctcgttat ccaggaaatc
    12841 acaccacggc ttgtactacg ttcgggtgat gccgtagtgg ggctgaaaaa taacggcttt
    12901 ggaaaagtac acgcgactga taattcaacc gtatctgaca aggtggagcg cgttgaaaaa
    12961 acagacaaca tctaatctat aaaaatataa ttatattttt atataaggac tcatatatga
    13021 ctgacgaaaa taaatccccc tctacggaag aagaaaagcc agtgacgaca cctgatcagg
    13081 atgatatcgc cacgctggaa cgagaagcca gagcaaaacg tgaggctgaa ttacaagcat
    13141 tacaggacga tgaaaaggag aactcattac aaccagcagt aaacaaactg aaaaaacgtc
    13201 cgaaggggaa agcaacggcc tttctggcaa tttgtgcagt agccctgatt ggcctgacat
    13261 ggggcggtaa ctgggtatat caaaacttcc tgacgcaaga agatggagag aaaacaaagg
    13321 aggttacgcc acctaaaaaa acgaatgact accggaaacg aaccgattta ggcatggcca
    13381 ccaatgagcc ggaaaattcg actgagggag agcaaactgt taaccaggat aacacgggca
    13441 atgtagccgg tactgccgga cagatgaaaa catttgagcc accacaactg aataaggcca
    13501 gtttccttgt tcgtcgtaat aatacggcca gcccccaaaa tcaggtcaaa acaagacaac
    13561 aggaaatgat actgactgcg gaaaacaacg ccacacaacc tacagatagt acagcaacca
    13621 caacacaaca tggcactgca tcacaggcaa tttcccccgc gacagtgcga agaatcccct
    13681 acaaccctga tttgtacata ccggaaaaca cagtcattcc atgctctatg gattaccgct
    13741 ttgtttccga tcgcgctgga agaatacgat gtacggtagc taaagatatc tggagtgcca
    13801 gcggcaatac aaaacttatc gagaagggaa ccacggcaac aggaatatac cagacgggaa
    13861 ttacgcaagg gcaagggcgc gcttttatca tgatgacaaa attacgtacc cgacagcgac
    13921 catatctgga tattccgctg attgatacca acgccgctgg cgagcttgga gaatcaggtg
    13981 ttgatggatg gattgatacc cacttctggg aaaggtttgg cggcgcatta atggtcggca
    14041 tgatcccaga tattggcgca tgggcatcaa acaatgcggg taaaaaagac cgcaatacag
    14101 actatacaga gaacagccgc caggctatgg cggatatggc tcgaaccacg ctggaaaaca
    14161 gcattaatat tccaccaacg ctttacaaga atcagggaga aataatcaac ctaattacag
    14221 ggcaggatat tgacttctca aatatctaca cgctgaggat gaaatagaac atgacggtta
    14281 aaaacatatc tcttgatcgc tataaacaac gtttttttag tgaatacctg gaattaccag
    14341 gactgacaga aatagctgtt aatcgtccag gtgaactctg gacaaagata gatggcgtct
    14401 gggggcaaca taccgttgat ctgaaatttg acgattgcta taactttgca cggtgtcttg
    14461 caaaacacca taacgataat atcgaggata tcaaaccagg actttctgca acccttgaat
    14521 caggagaacg atgtcaggtt gtcgttcctc cggcgtgcga acgtgacacg atctcaatca
    14581 cgatccgtaa accgtcaaaa attcaaattc cgcatcaggc atatattgat agcggttttt
    14641 ataaccgcgt cacgggcatt gaaaaagcat ccacacataa cgaagaactg attgaattat
    14701 acaaatcaaa aaacattcct ttgtttatgg aaaaatgcgt ggagtatgga aaaacattgg
    14761 ccgtcgcagg tgaaaccagt acgggtaaaa caacatacat gaaaatgttg atacaatata
    14821 tacctgtcca tttacgcata actacgatag aagacaaccc agagataata ttctacaaac
    14881 ataagaacta tgttcatctg ttttatcctt ctgaatcatc cgatgaaaaa ggatctgttg
    14941 taacaccagc agcgcttata cgctggaatt accggatgaa tccagacaga attttattaa
    15001 cagaagtgag gggtggagag gcatgggatt ttctgaaaat aacggggtca ggccatgaag
    15061 gaagtatgac ctccattcat gctggttcag cacatgaagc aatcgacggt tttattaccc
    15121 gctgtactga aaacccacag tgtgcgcaat taccatattc attcttgcta cgtaaagttc
    15181 tcgactctct ggatgttgtt gtgagcattg atttagatgg aaacatacgc cgtatgaatg
    15241 atatttattt ccgaccgtta caccgcaatc aatattacga ggaaatgaaa gtatgataaa
    15301 gcatatactg gttactgtgt ttgtaacact aattaccgga tgttcctcac cgcctccacc
    15361 agtacccgtg gaatggaata aaaagccggt atcgcttaac gataaacttc ctgaatggaa
    15421 acccaatagc ttaataataa aatctcctgt tattaatgat aaatggacgt tatccattag
    15481 ctcacaagat ttcaataaag aagattggat gccatctgtc ttttatgcca ttgctcactc
    15541 aacacaaatc acagtagcaa caaagtcagg cgctgattat ttcagcacaa aaaactggtt
    15601 gtatcatcat ggcgcaaacg gggtaattat atatcacccc atcataaatt gtcttacgtg
    15661 caatgaaaca accatctact tttcgcgtta acacaaatat attaaacaaa gggttctgac
    15721 atgaaaaaaa ttgcagcaat aacaatgttt gttttaacaa ccgccacacc tgtttactct
    15781 tatgccgcag acccatgcga ggttgtttta tgtatgtacg gaaaagctac aggaaacagc
    15841 ggaggtggcg aatgcagatc agcagaaaaa gcattcttca acattcttaa gaaaaagaaa
    15901 ggttcaatac gatggagtaa aacatttgat gcacgtaaag catttttaaa tcagtgttca
    15961 tcagctgatc ccgcagccat atctaaaata atgagtaagt ttggtagagt ccgaggataa
    16021 atatgaatac agatgaaaac atggtatttt atctgacaaa ctccgatctt gtttatagct
    16081 atgcacacaa gatagtacac tccattccat caggaatgaa aggaaaattc attacttcta
    16141 ttggtgaaac tattgaagat ttaaagaaac gacatcctga tattattctc attacagaat
    16201 ctgaagcatt agcattgcaa tcggagagga aaaattaaca tattctttca gatctggaga
    16261 atcctatgtt aaaagaagat atgttcatta ataatattca ttcaagaaat caggacagaa
    16321 tccatgtaaa aagagtcttt gatgatatta gtaataaagc aaacagggga tgcggactat
    16381 attatgaaat atatcaagcg aggttaatgc gagggttaat tgagcacaca gtaaaactga
    16441 aaagttctga tgccaataag ctgattagtt acgctgcttc acaaggttat gaaataacag
    16501 aagatagata taatgctgta ataaaagccg aggatgaatg tctgactgaa ataacacggg
    16561 cacaagtctg aggcagagga ttgcgtagca acccgaaggg cggggcaaat tcgctttgcg
    16621 aatttgggcg agtccgacgc agggcgtggt gcagttcagg ctttctttat ttctggcttc
    16681 aatttcgaag aaattgttaa gacaggaata aagaaagtgg tgcgtggaca gaaaattcaa
    16741 tttcgaagaa attgttaatt ttcaggcacc acgcccaccc gcgaacattc gccccaagcg
    16801 aattgaagcg ggccgggggg agccgcaaaa atttttccgc cccagcgaga aaaaaggcac
    16861 gcccacgtgc cggtttttgt ggggggcttg ccccgcacac cgtcgggcca ggtaatattt
    16921 cccgccccag cgggggatat taccgattag gcgcgaccac cccctttaaa gcagcgttcc
    16981 aattttttcg agcttgcgaa gaaaaaatag gctaaacgcg cgtcttaaag ggggtggtcg
    17041 cgcgtagcgc gcgacggtgt gccgcctttg accttggggg ttttggtccg gcgtccagac
    17101 gggccattac ccccatgtga cgtgaaggac tggcgttcca gccaggccga aacggcacac
    17161 gccgttgccc gttattcggg ctttcaggag ccagacgata acttatcggc tggattctga
    17221 aagaacggat aacgggcatg atcgcctaag gcgatcacaa tgtttttggc ttttattggg
    17281 gtacaacaat ggggtacagc tatggggtac atcatatccc ccattactgt accccattgt
    17341 tgtaccccat agattcactt tatgaatggg gaaaacaaat atgaatccac atctaatgca
    17401 aaagctgaat ggtattgctg atgccttttc aatatttctg ttatggctac agaacagccc
    17461 tgtcatattg tcattactgg caggactaac actaccattt atattcaacc taccacgaga
    17521 ggaaagaaaa aacgctccct tctggttaaa atctgttgcg tgcgtgtcca ttttcttttt
    17581 catattcggc acaatgtcac cacttagtat tcatggtctg agctacctat ttgacgttct
    17641 cgatcccaat acactactta gaataccatt atggataatc acaattctct ttacactagc
    17701 cggattagct tttcatttga ccgcgcgacg attattagca ggtgaaatag acagcctgaa
    17761 acacagaatg ataaagaaaa gcaaacttga gagaaataca agaaccgatg ttcgcaaagt
    17821 aaaagaattg ttgccagaca gcatagaata caatccccta gattacattg atttgaaaaa
    17881 aggggttttc attggcttag acaaagacga ccaaccgcaa tatataacaa taaaagaatt
    17941 tcagaaacaa catgcagcaa tcataggtac aacaggttcc ggtaagggca ttaccgcatc
    18001 ggttttgctt tatcaggcaa ttctgttagg cgaggctgtt tttgttgaag atccaaaaga
    18061 tgatgaatgg gctgtgcatg ttcttaggga agcctgtaaa aaagcaggaa agaaatttgt
    18121 tgtaattaat ttaaacagaa ccaaccatca acttgattta cttgctgaca tatcacatga
    18181 acaattagaa gaattattta atgcaggatt tagtctatca aaaaaaggag aggccgcaga
    18241 tttttatcgt atagctgatc gccgcgcagc cagaaatgta gccgcaattc ataaaaccgg
    18301 aatgacactg tatgatttat tcaacgatga caacgtaaaa tcaataggag aagatgcccc
    18361 agcattttta ggtgaactag aagaaattgc tcttgtaaat tccgtcaatg caaaaaaagg
    18421 attttctctt aaagagattt ttgataatgg aggatgctgc tatatcattg ggtcaacccg
    18481 aaatcaaaaa attatttctg cacaacgcat gatacttacg cgtttgattc agatagctga
    18541 aaccagagac agaataaaca caactccacg tgttgttgca atttttcttg atgagttgaa
    18601 atatcatatt tcacgcccga caatggaagg gttaggtaca gcacgagata aaagtgtgca
    18661 tatatttatg gccttccaag caatagatga tttaagagat tgcccttctg atcttaatgg
    18721 tgattctgta attggtgctg ttattgaaaa cgcaaaattc aagttaatct ataaaatcca
    18781 gaatccagaa acagcagagt gggcagctaa aatgactggt tcaatattag ttgacgatga
    18841 aatgaggaag gtgagaaccg acttatcatt aacagaaaaa gttgatactg accgcatgat
    18901 aagacaggca gaaagttatt atgtagacag taatatgttt ttaaatctac ctgaaagagt
    18961 aggatttgtg ttcacatcaa aagatttacc aaaagcaact aaaatgtctc atataccagc
    19021 aaacaaagaa accattgaat tgctatcttt tgagacagaa caaatagaac aacacaataa
    19081 caacgatgtg ccttccagtg gtttatgagg ttttactatg ttaattcatg atttttacga
    19141 aagaaaaaga atatcagcta acaacatgct ttgtctttta aatttcctta aggatgaaac
    19201 gtattcagac tttcacatta taaaaagaaa attaggattc acaaaaaaaa gcagaaaccc
    19261 agtatataaa ttactaaaca aggctcttaa agaaggtttt attatcaaac atgaaatacc
    19321 atcatcaaaa agaaaattaa ctatttgggg tattactatg caaggattag caatggttgt
    19381 aaaagccgat gacattacgt tccctagtta tttcgaaccc agcaaactaa aatactggac
    19441 tcttgagcat cgtcttttaa atcagcgagt gagaatatct ctggaaagta aaggcgctac
    19501 tgaatggatt aacggtgata gtgcagcctt catcgcacgt tattccggct gttcccatcg
    19561 ccctgatgga gtaataaaac aaccaactgg cgcaataatg gctattgaaa cagaacgaac
    19621 aatgaaaact cgtgctcgct atataagcat tataaacagt catcttacgg caagagataa
    19681 aggaaactgg cattatgctt tgtacgtcat gcctgacacc aaaagtaaag aatcattaat
    19741 cagactgttc gactcaatca aaactgtaat gaggaataat acacctgtgc cttttgatga
    19801 aaaaaacaga gagatgttta tttttagaac aattgatgaa ctggaacaat agttgatgaa
    19861 aatattaatc atattcgcat tatatatctt gtcgccttca ctatatgcca atgcagcgga
    19921 tatacgggga aaagttattc gtgtacttga cggcgatacc atcgaaattt tacagaataa
    19981 aaagccagta aggattcgtc tggccaatat tgatgcaccg gaaaagaaac aggcttacgg
    20041 acgctggtca actgaccaac ttaaaagcct gattgccgga caatccgtca gggtgacata
    20101 ttcacacaac gaccgttacg gtcgcattat cggacgtgta ttcacaacag gcggtagtga
    20161 ggctaaccgc tttatggtgc aatcgggtgc tgcatgggtg tatgagcatt acaacacaga
    20221 taacgctctg gcagatctac aacaggaagc tcgctcacaa aaacgaggtt tgtgggctga
    20281 ccccaatccc gttccaccgt gggaatggag acataagtat aaggtgacaa aatgaatgat
    20341 tgtttcggtt gtatgcatcc atatcccgcc ggtcactggt tttattcttt acaggattta
    20401 cttactccac tactggaaaa tcctcttttc tacgtctttt cctttttttt cgcgggtatg
    20461 ataggtgcaa ttatatcaag aataaccaaa ttaattaaaa cgatgaaaca tagcaataac
    20521 aataacaata aaatatcaat agataattat gaccaaattg aaatagaacg ctatatgaca
    20581 aatgaagagg ttggcagatt agtaatcaat tttatgaaac gcaccaacgg agaaccgtta
    20641 ctaatagtgt tcccagaata tgacaatgga gacgataatg acgataaaaa tactcgttgt
    20701 atcaaatgac ggccatgaaa aactaattga tctaagtcca gttaatgatt tagctaaaat
    20761 tacaaaatca ttgcgtacat cagaaaacag gatggtatgt gttatccagg acaataatcg
    20821 tattctccgc tgggacagaa actatgcctc tcgtgcaaaa aatcactggc gaaaggtagc
    20881 tcccgaccgt tttgagattt taggaaccat tgaacatatt cattatgttg gtaagtgtta
    20941 atatcagggg acagttcgtc ccctatgcca ttttaatgat gctttttcca gaattcctgg
    21001 tacgcctcca gcaactcccc gtgcttcata tcattagccg ctgcaatcga gcggaactcc
    21061 cgtttaactt ccatatccac ataaaggtta agctgagctt ttctcccatc agcgttttta
    21121 cgcgtcggat taaatccgtg ttgtgtatcc tccagacttg gcggctcgcc tttggtgtta
    21181 cgcggttttg gtggtacatt agccatctta tcacctctat aaaaatataa ttatagaatt
    21241 atatatcact gtgagagttt agtcagcaag tcaataactc cctggacaac ttcgtcagct
    21301 ttctgccgtg gacctggaaa aggggattcc gtcaatgcca gtcctttatc ctgtgcctgg
    21361 ctgaacgagg tcataaaagg tattgcgcca ttgataacct gaacaccaca cggtttaaga
    21421 tattcaagcg cggtacgata ctcactttcg ctttcactga ctccgacaaa aactacagcc
    21481 atcttatttg ttgagtgtcc cttgttacgc aatgcattca cggttgtaac cgtcgttctc
    21541 aaatcgtcca cactaaaacc agacggtaac acgagcaaat caacaatttc tgccaggctc
    21601 aacagcgatt tagttgcata agcagcacta tcgataacga ctaaatccca tatccctgat
    21661 tcaatatgtt ttttagcctg tgataccgtt ccgcatccct gaatagtgac aggtggttca
    21721 actttatatc tcaatcgccg ctgtccccaa tccaggaatg acccctgatc aacatccata
    21781 tcaagcccta ataccttcca gtctgccttc gtgtactctg tggcaatagc cctgctcatt
    21841 gaggactttt tcaccccgcc tttaccgtta accagcgcaa tagctaccgt catacgaccc
    21901 cctataaaaa tatttttata gaaaaatata attatagtat ccatattggt gccaagaatt
    21961 gcaacaacta tttgaactac aggcggcaag tgaagagata acttgtgaat ataaggcttt
    22021 cacaaccaaa ttcaggcaaa acaaaaaatg acgttttatt aacaaatacg caacacaaaa
    22081 aacggtggaa aacgcccagg aaaacatgat cttttgagcg gattttgcag atccgacaac
    22141 gaacggtgat ccgatggtgc cgataacgtc cattatgtta aatagcctcg tttttattga
    22201 aaatatagtg tattcgttat aatattagat ataatgaata agctataggt tttttatgtg
    22261 gaacgtagta actacagata tctttgatgc gtggtttttg acgcaaaatg aagatcttcg
    22321 tgaatcagtc tatgaggcta tgggattact ggaaaaattt ggtccaacgc tgggaaggcc
    22381 atatgtagat acattgaatg gttctgattt tgccaatatg aaggaactcc gcgttcagta
    22441 taaaggtagc cctgtcagag cattttttgc atttgatcct acccgtaacg ccatagtgct
    22501 ttgtgcagga gataaaacag gattgaacga gaagcgcttc tataaggaca tgataaaact
    22561 ggctgattcc gaataccgta aacatcttgc aaaactggag aaataacatg ggcacctata
    22621 gagaactact ggctaaagag agtcctgaaa tgcaggcgcg cgttgccgaa cgggttgaaa
    22681 aagccagcgt tcagatcgca cttagtgctt tacgtgatga actgaacata tcccaaaccg
    22741 aactggctac aatgatggga gtaaaacaac catcagttgc caggatggag caaactgaca
    22801 acgatccgag aatatcaacg cttaaacgtt atgtgaaagc gttaggcggc gagttaagtc
    22861 tggacgtaac attgcctaca ggaaagcgag ttgctttcca cctgtgaaaa cagatgagaa
    22921 cattagaaga agtaattgcg gctcaatcac ctgaaagtca ggctatcatc aaagccatgg
    22981 ctaatgagat gattctggaa acaggactgc aaatgatgcg tgaataattg cagcttttta
    23041 ggagtgaaag ccgaaaatgg gcgaaaagtt cattttcggc gaattaattg tattatcctt
    23101 aattatttaa agtacccaca gtatcccttt tacaaagaac atattcaggg tgttttccag
    23161 ccaacaacaa taagtagtta tattcaccta cagataaaga ccttgtagct ttacctgcgc
    23221 tttctttcat ttgccaggta tttaactgat agccgaaaat ttcggcggct tccttaagcg
    23281 ttaaacctgc ggaaactctc gcttctttaa catctgccgg agttggttta gtttgcattg
    23341 ttattacctc ttgtaaataa atcagccaga ataccattat caaagctcaa taattctggc
    23401 aatgtaagat tcgtatcaag caccaatttt ttaactgtct ttttcccttt aacttgctct
    23461 ttatcacaag atttcacata tgtcttaaca tactttctga ccatatacac ctgattatcc
    23521 atatctactg taacaacata agagtaacta cccttacttt taaagaaaac attatcagtt
    23581 ttcataaaca accccatata aaaattcaca tcaacacttt gttatagttt ttttataaaa
    23641 cctgccactt tgataagttg atcaacatta agccctctga taaccatttc ataatcatca
    23701 ccattttgta gtatttttcc tgcaagtcca ttatccgcat taaatcccgt taatgcccca
    23761 taccagggag atatagaaaa caactgtgat aacatccctt tgattattcg cactttatct
    23821 tgtttttcct gtaatgcgct ctcatgctcc ctgagcaatt caatttcatc cagtgcatta
    23881 gcaaggattt tccgagatat atcattagca ataccaacag gattgcgcgt aagagataca
    23941 gtacagacgg aaggattacc gtgccctaca agactatcaa cgctaccaac tataaaaaat
    24001 ctttcacgct ccagacgaat aatgatggag tatttacgaa tttctgtaga agtcagttta
    24061 atacgatgct gataattatc cagtttgtta acacgccagc cgttcccaag aatacggcat
    24121 acaacttcaa agacaggctg atatttttct gaaaatgttg tcatgctttc acatccttta
    24181 ctgcgctaac gtagccagca atcgcagagt gtcagctact caatcctttt cgggttcgat
    24241 gcccgaaaag gattgctggc gag
//
