LOCUS       NZ_CP101122             4167 bp    DNA     circular CON 25-AUG-2023
DEFINITION  Klebsiella pneumoniae strain BA32726 plasmid p_unnamed, complete
            sequence.
ACCESSION   NZ_CP101122
VERSION     NZ_CP101122.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29409900
            Assembly: GCF_030863585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Veeraraghavan,B., Shankar,C., Teekaraman,M. and Shanmugam,S.
  TITLE     Carbapenem resistant K. pneumoniae BA32726
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Veeraraghavan,B., Shankar,C., Teekaraman,M. and Shanmugam,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Unicycler v. December 2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/23/2023 16:06:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,620
            CDSs (total)                      :: 5,494
            Genes (coding)                    :: 5,369
            CDSs (with protein)               :: 5,369
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 47 of 125
            Pseudo Genes (incomplete)         :: 74 of 125
            Pseudo Genes (internal stop)      :: 18 of 125
            Pseudo Genes (multiple problems)  :: 12 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101122.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA32726"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p_unnamed"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2021"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College and Hospital, Vellore"
     gene            join(3543..4167,1..491)
                     /locus_tag="NLA63_RS28075"
                     /old_locus_tag="NLA63_27995"
     CDS             join(3543..4167,1..491)
                     /locus_tag="NLA63_RS28075"
                     /old_locus_tag="NLA63_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            491..1123
                     /locus_tag="NLA63_RS28080"
                     /old_locus_tag="NLA63_28000"
     CDS             491..1123
                     /locus_tag="NLA63_RS28080"
                     /old_locus_tag="NLA63_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            1489..1731
                     /locus_tag="NLA63_RS28085"
                     /old_locus_tag="NLA63_28005"
     CDS             1489..1731
                     /locus_tag="NLA63_RS28085"
                     /old_locus_tag="NLA63_28005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2077..2478)
                     /locus_tag="NLA63_RS28090"
                     /old_locus_tag="NLA63_28010"
     CDS             complement(2077..2478)
                     /locus_tag="NLA63_RS28090"
                     /old_locus_tag="NLA63_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2478..2669)
                     /locus_tag="NLA63_RS28095"
                     /old_locus_tag="NLA63_28015"
     CDS             complement(2478..2669)
                     /locus_tag="NLA63_RS28095"
                     /old_locus_tag="NLA63_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3085..3198
                     /locus_tag="NLA63_RS28100"
                     /old_locus_tag="NLA63_28020"
                     /pseudo=""
     CDS             3085..3198
                     /locus_tag="NLA63_RS28100"
                     /old_locus_tag="NLA63_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          2791..3198
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gcctactaac acaaaaaagg aaaaaagaca tactagaaaa cttaaaaaaa atagatgaca
       61 gaattgtaga tatagcaata tctgcgatag gtaacaataa ggaaatatat ttagacattg
      121 gatttagtga gctaaatgaa atatcaatgc ttggcgaggg tatcagtcga gcattatctt
      181 ttatcagctc agtgctggta caggaaaact caattattct tattgatgaa atcgagaacg
      241 gaatacatta ctcagtaatt aaagacataa taaaatcatt aatttcatct gcaaaacaaa
      301 acaacaatca aatatttgca acaactcata gtcaagatgt gatacgagca ataaacgaaa
      361 tagatagcaa aaatgaagat atcgcttata ttagactagg aagagaaaag aactccctaa
      421 aaccaacagc cgtacaattc aatatggatg atttctccta ctcagttgag aacgactggg
      481 aggtaagata atgaaagaca taaataatag caaaaagatc attttcgttg agggtgatga
      541 tgattttcat tttctatgca acctacttga aatacaaggc attaccgatg tttttgtcga
      601 aaaaataaat ggaaaatcaa aattaaaaca agcacttaga gcattcaaaa acattgattc
      661 tttcgatgaa aaagaaagta tttttattat attagatgct gacacatcat tttctgacac
      721 agaaagatca ataattgcca cattgcgaga actacaaatc tcctccccca gctcgcataa
      781 cgagatagct atgaatggca atacaaaaat ttctttcttt ataatgccag gaaaagacaa
      841 gcaaggtgcc attgaagatc tgataattga acacgcaagc actcgagcgt tatatgaata
      901 cataatagaa tttttcgaaa aaattaaaaa taataaagat gacatctcag cgagtgacaa
      961 agagtacact tatccaacaa acgaaaaaaa agctaaggta caagtctatc ttagctgcaa
     1021 tagagaatcc gactcaagaa taggaatatc catgaaaaat ggaataataa acacagcaga
     1081 caattgcttt gatgaaataa aagattttat ttcaaaaata taaatccaaa tcaagccagc
     1141 ccttcacggg ctggcttatc accagggtgt tattaaacgc cggataacgt atagaaagca
     1201 tcatacactt tagttatcag caatctcaaa cgtcctaaag acatcaccag atgccaaaac
     1261 aatgattgtg cagatctaat gtaaaagacc cagcacaaga tgctccctta agttatattc
     1321 tctctattca cttaaaacaa tttaacataa aatcataatt gtacgcaccg ctaaaacgga
     1381 ctcaggacgt ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc
     1441 agaaacgcgc aggctctgtt tttacggcat gcatgagtat gcgcgcaagt gcatagcgcg
     1501 gaaattcaca ccattcagcc aggggaagtc ctgtttccgg cgttcttctc ctccgaaaac
     1561 ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac
     1621 cggttttcag caccttcagg aatcgggttc gtcgacggcc ggcaatcacg ttatggcaag
     1681 tcgccggacg actcaccaca cgattcaccg gcgattcgaa tcgtgaattg aatcaagagt
     1741 caccatgagc aaaaaacacc caaagtgatg aattgaatca agagtcgatt catcataaca
     1801 atatgataat aaagcgtgat tcacatatga atcgtgggca attcgtcagc gattcgccat
     1861 agagtcgtcc acgacttgga tctgcacttt gtaacacatc accgcacatt tatttttcat
     1921 taacccattg ttgtaaataa ggaatctgca aaattttaat tttgcgtggg gtgtgggtat
     1981 ttttagtggt gaggagcgtt cagagatctg acgatttttg ggaaggttca ggaactccct
     2041 gggatggccg gagaggaaaa ctgaagcatg acggggtcag gattttttcc agaatttcca
     2101 ccatggcgcc ggggattcag ttaattttgc ctggtgattt tcaagaagga gcatggcctg
     2161 tttcaggctg tcaatgtgcg aatccttgga caaaagtaag gtgttcaggt gttcaatctc
     2221 ctgtttcaga tgacggatca cctgttcact ctggtcagca gaggctgtat cacgctgtcc
     2281 agagacctgt ccagaatggt gttcaccctt cggttgctca aaggggccaa agacacgtaa
     2341 aagctctgaa gtatctacca tttttgcatt gcgctcaccg ctacaggtgg acaggtcacc
     2401 tgctttaatt aacctgtgca gtgttgttct actcttaccc gtcagtcggg cagcctcact
     2461 aatagacact ctggccatca gttaccatcc tgtaatgcat ccagtctcga ggacagtgta
     2521 cggaaaagat gctccgcatc atcgtggagt ttctcacaaa ggtcggcctc gacatcgaga
     2581 tcaagctcgt taagcttctc cagcaggagc agagactgat tctggatgaa tttagccatg
     2641 ttcagcgctg ttttctgttg tttcgtcata agaggcctca gagcgtcagt ggtcagtgta
     2701 gggatctcct cttttccgct tcctcgctca ctcagtcgct acgctcctgc cgttcggctg
     2761 cggcgagcgg caggagcatg cgtagaaacg ctcgagaaaa ccgagtaatc gcatggagga
     2821 aagaaggaaa ggccgcacag cggccgtttt tccataggct ccgcccccct gacgagcatc
     2881 acggaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     2941 cgtttccccc tgatggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     3001 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
     3061 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc
     3121 taatccggta actatcgtct tgagtccaac ccggaaagac acgacaaagc gccactggca
     3181 gcagccactg gtaactgatt tagggagtta gtcttgaagt tatacgccag ttaaagctaa
     3241 actaaacgaa cagttttggt gactgcgctc cctttaagcc agttacctcg gttcatagag
     3301 cgccaactct actgaacctt tcgaaaaaac cgcccgccaa ggcggttttt ttcgttcaca
     3361 gccggaagct tacggctgct gccagataat ctcaagaaga aagtaaaaaa aatcgattaa
     3421 aacacaagca atcaaagtta atctttgaca taaagtctag ctaatcacaa tgaaagctgt
     3481 acattttatt aaggtactaa caaatcgtat ggcatataac aaaaacacga acagaggcta
     3541 tcatgataaa ggaaataagt ttaaagaact tcaaatgctt caatgagcta tatctaaagc
     3601 aattaaagac tttaaatatc atagctggga aaaacaacta cgggaaaaca tctatactgg
     3661 atgctatttt ttgtttttat gatgtaaaaa acccggctgt tttgttaaac attcaagcat
     3721 tcagaaaaga aatggcagaa ataaacaaaa acaagccatt ttgggttagt tactttcatg
     3781 atatggacac atcacaaaag atgtccatag tcattcgaga tgaacgctct gaagtaacac
     3841 aaacatatga aacagaaaca aatcagagat tagaatcaag tttgtctcta aacgttgaca
     3901 ccgtactatc taaccaaaac atacctcgtc aaactatcgg ttctcaggcc acagtaaact
     3961 ctttaaaaat aaatgtgacc gagacaccca atgcgaaaaa cagggtttcc aataagcttt
     4021 tctctatgca attgaaagaa gatggcgtag aaataaaatc cgaaataaaa aacgaaaggg
     4081 atgggattaa ttataaattt aagacagcaa caataataac aacatccaga aagataaaca
     4141 aagaggcaac aattacaaat gtatcta
//
