LOCUS       NZ_CP100731             4593 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Blockley strain R17.0776
            plasmid pR17.0776_4.6k, complete sequence.
ACCESSION   NZ_CP100731
VERSION     NZ_CP100731.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29431217
            Assembly: GCF_024266545.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Blockley
  ORGANISM  Salmonella enterica subsp. enterica serovar Blockley
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Chiou,C.S., Chen,B.H., Wang,Y.W. and Teng,R.H.
  TITLE     28 Salmonella enterica strains that have azithromycin resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Chiou,C.S., Chen,B.H., Wang,Y.W. and Teng,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.9; trycycler v. 0.5.3; medaka v. 1.6.0;
            homopolish v. 0.3.3; polca v. 4.0.5; polypolish v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 287x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/23/2023 02:18:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,000
            CDSs (total)                      :: 4,877
            Genes (coding)                    :: 4,749
            CDSs (with protein)               :: 4,749
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 55 of 128
            Pseudo Genes (incomplete)         :: 89 of 128
            Pseudo Genes (internal stop)      :: 19 of 128
            Pseudo Genes (multiple problems)  :: 31 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100731.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Salmonella enterica subsp. enterica serovar
                     Blockley"
                     /mol_type="genomic DNA"
                     /strain="R17.0776"
                     /serovar="Blockley"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:57741"
                     /plasmid="pR17.0776_4.6k"
                     /geo_loc_name="Taiwan: New Taipei City"
                     /collection_date="2017"
                     /collected_by="Centers of Disease Control, Taiwan"
                     /subtype="ST52"
     gene            856..1695
                     /locus_tag="NL731_RS24940"
                     /old_locus_tag="NL731_24940"
     CDS             856..1695
                     /locus_tag="NL731_RS24940"
                     /old_locus_tag="NL731_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(2383..2616)
                     /locus_tag="NL731_RS24945"
                     /old_locus_tag="NL731_24945"
     CDS             complement(2383..2616)
                     /locus_tag="NL731_RS24945"
                     /old_locus_tag="NL731_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_049264670.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFVTNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2623..3183)
                     /locus_tag="NL731_RS24950"
                     /old_locus_tag="NL731_24950"
     CDS             complement(2623..3183)
                     /locus_tag="NL731_RS24950"
                     /old_locus_tag="NL731_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3218..3652)
                     /locus_tag="NL731_RS24955"
                     /old_locus_tag="NL731_24955"
                     /pseudo=""
     CDS             complement(<3218..3652)
                     /locus_tag="NL731_RS24955"
                     /old_locus_tag="NL731_24955"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3861..4184)
                     /locus_tag="NL731_RS24960"
                     /old_locus_tag="NL731_24960"
     CDS             complement(3861..4184)
                     /locus_tag="NL731_RS24960"
                     /old_locus_tag="NL731_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4247..4438
                     /locus_tag="NL731_RS24965"
                     /old_locus_tag="NL731_24965"
     CDS             4247..4438
                     /locus_tag="NL731_RS24965"
                     /old_locus_tag="NL731_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          29..584
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.907"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2286..3871)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtggcatcag tgcggattat aagaaaagtg caccatatgc ggtgtgaaat accgcacaga
       61 tgcgtaagga gaaaatgccg catcaggcgc tcttccgctt cctcgctcac tgactcgcta
      121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaagacggt gatgcggtta
      181 tccacagaat caggggataa cgccaaaaga aacatgtgag caaaaggcca ggaacaggaa
      241 gaaggccgcg ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      301 cagatgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt ggtatctcgg
      481 cccggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac acgtaaaaac
      601 gccactggca gcagccattg gtaaccgttt agaggagtta gtcttgaagt tatgcgccgg
      661 ttaaggctaa actgaaagga cagatttggt ggctgtgctc ctccaagcca gttaccttgg
      721 ttcaaagagt tgctgctctg agaaccttcg aaaagccgcc ttgctgggcg gttttttcgt
      781 ttacggacaa gactaccttc aggatcgtta gatctcaaga caatcatgca tagtgagcat
      841 acacaggagc cgaacttgct tacactgttc atcttttttg ttttattaat agctgcatgt
      901 ttcttttgtt ttgctccacc acgaagagga tatgacagaa atgagatcat tccatacaaa
      961 ataaaactat caataaataa atatcgcctt tacatatact catcgggaaa ggtaagacaa
     1021 taccttttat ttttagttat tttatcactg tactactcca tagcggaacc atttaagtct
     1081 gagttaataa aaaacatatc atattcactg atggctgcat ttatttttga tacagggctt
     1141 aattttagca aggagaatat aactaaagga gtgatttcaa caaggtggca taatgaccta
     1201 tattcatcat ttgagagaat gaaagcaatt aataaaattt actacccatc caacaaagag
     1261 ataaacacag aaggtctatc caaagccatc acatcatctc ttttcaatga tgacgcgaac
     1321 tcgttcgcaa agagagattt tcgcttgatg tgggacttat cttctgaaaa atatctttcc
     1381 tataaagaaa taataataag aaaaggcgat aagcttgacg ctgtatgctt gagatttata
     1441 aatgatgact acaagttttt agttaatttc aacagagatg aagaggtttt caaatacttt
     1501 ccatctataa tgcaaccttc actcaaaaca tatagagcgt tatcaagact agtaaactca
     1561 atcaaagatc cttctagatt taagttcacg acagagtcat tagaaatgga gttactagaa
     1621 tacttagagt taagaaatga attattcaat gacatagaag aagtaatggg tagctatgct
     1681 cagagggcac catagttcat gatagccctc gatagtactt acttatattt ttagagaggt
     1741 ttaagtgtaa gtagagggag taacacctgg tttttcagca tccactccaa tgaatgctga
     1801 caccgatacg cgataattct ctttatcgta gcaaaatcat cctccagatt gcggttgaga
     1861 ataggttcat ggtgcgcaat acgattgcgc agttttctca cgggacttgt tagcagtaat
     1921 gcttcccccc ggggacaaac gacagagata tacaacaaag gcccgcaatt aaaatactcc
     1981 gtatttacca taacgtcgca ttgcgcgtac cgcttaaacg cgttcagcgc gatcacggca
     2041 gcagacaggt aaaaacagca acaaaccacc cgaaaaactg ccgcgatcgc gcctggtaaa
     2101 ttttaacctc atgcatagtt atgcgcgaaa ctgaatgacg atcagtttcg cacgttccag
     2161 ccgtcgaacg gggcaaaatg gggaggtttg gatattgtta atgctaatgt acgtacatta
     2221 acattggcgc catccgggta gggcgtgcca caaactggct cgatttcagg gggtaccccg
     2281 gtcacatccc cattgacggg ccacgccaga tccgcgcttc gggttcctgc tcttcatgcc
     2341 agcgccgggc aagctcctgc gcctgacgct cctgttcatc ccttatctgt tgttcgtgac
     2401 aaataaccga ctcaagcggt cgacttgtcc ggctaatagc tgcacctgct gactcaagtt
     2461 cacgacacgc tcgttcagta tcgcgttgtt ctgttgcgta accgcgtgca tcttctgcaa
     2521 ttctgcgaag gcgctctccc actcgttcag ccgccgcata tagtcctgtt gcaaatgctc
     2581 taaggcgttc agcaaatgcc cttccagttc cgtcatactc gtttactccc tgaccagcct
     2641 cacggcgttc ttgtcgccca ccgtccaggt gttttcccct ttcaccccgt ccggcagcac
     2701 cagaaatttc ccgttctcgc cattgacgaa cgtcaccccc caggtttttg cctcgagttt
     2761 cgccagcgtg ttttcctgct ctctgatggt caagatattt ttcgcaatcc tgctttcctg
     2821 ccactgaatc agcccccagc accccaggaa aatgccgccc gtgacgctga gcgcggtcag
     2881 cgccggaaac agtacccgcc ctttgatcac ctgccagagc aggtttttct gctgccggga
     2941 cagttcggcc tctgtggtgc tgagctgcgt gctcacggtg gtgctcagcg tatccagttg
     3001 ctgtttcacg gatgttgtat gttcgctgat agcgtctctg attttctgcc tgtttaagct
     3061 cagtgccttg tctacagact cttctagctt cctgaactcg ctgttcagca tgttctcggt
     3121 agaagcggct cgctctttca gtttcttctc gaagtccgtt cccatttgta aaagattgct
     3181 catacagcgc cccctttagc ctgagattcc gccccccctc cgggtcggca atactgatac
     3241 tgcttctggt tgtcctcacc acctcaaaac ctgccgctgt gagcgcctca gtgacatcct
     3301 gacgcgtttt tattgctccg gcacggtaaa gcgcctcaag accccgtgta atcgcttctg
     3361 cggcctcctg tttcgctttt ggcagattat tcggtgtgac aagcgtccgt ctgttctccg
     3421 gtgcgttcgg gtcacgcagc ccgtaatgat gattaaccag cgtctgccag gcatttatcc
     3481 tcggcctgtc cgcacggtcg tagtacggct ggaggcgctt accgctctgt agttccatgt
     3541 tcggaatgac aaaattcagt tcgagccgcc ccttatcctg gtgttccacc cagagaatgc
     3601 tgtactggtc tttttcgaga ccgggcataa gaacacgctc aaagctcgtc atcactcttt
     3661 cgcgttcacc cggcggtaac tccttctccg cgaacgacag aacgcctgac gtgtatttct
     3721 tcgaaaatgg cgtggcatca atgagttccc ggatttcttc cgggttaccc cggagcaccg
     3781 ttgcgccttc gcggttacgc tcccgcccca gcaggtaatc gaccgggcca cttccaccgc
     3841 ctttccccct ggcatgaaat ttaactatca tcccgtcccc cctgttccct gacagcctgc
     3901 cgcaactggc gcaattcacc ctcgatggcc atcagagcgg ccaccacatg aacccggtca
     3961 atcgaagacc agtggccgct gttcaccttc cgggctgtct ggttcagatt gttcccgatg
     4021 gcagccagat gccgcagcaa tggcggcgat agtgtcggca gttttcctgt gcgcgcaacc
     4081 ggctccccca gacagacccg ccgcatccat tctgccagtc gtttaccctc gcagcgttcc
     4141 agtaaccggg cgtgctcctc atcgctaacc cgtatcgtga gcatcttctc gcgtttcatc
     4201 ggtatcattc cccctaaaaa atcccctatt aaggagcctt aagaccatga ccaaacagga
     4261 aaaaacagcc cttaacatgg cccgctttat cagaagccag acgctgaccc tgctggaaaa
     4321 actcaacgag ctggacgcgg acgaacaggc agacatctgc gaatcacttc acgaccacgc
     4381 cgacgaactc taccgtagtt gcctcgcacg cttcggcgat gacggtgaaa gcaactgaaa
     4441 catgcagctc ccggagacgt tcacagcttg cctgtgagcg gatgccggga gcagacaagc
     4501 ccgtcagggc gcgtcagcgg gttttagcgg gtgtcggggc gcagccctga cccagtcacg
     4561 tagcgatagc ggagtgtata ctggcttaac tat
//
