LOCUS       NZ_CP100714             4593 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Blockley strain R18.0186
            plasmid pR18.0186_4.6k, complete sequence.
ACCESSION   NZ_CP100714
VERSION     NZ_CP100714.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29431224
            Assembly: GCF_024266465.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Blockley
  ORGANISM  Salmonella enterica subsp. enterica serovar Blockley
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Chiou,C.S., Chen,B.H., Wang,Y.W. and Teng,R.H.
  TITLE     28 Salmonella enterica strains that have azithromycin resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Chiou,C.S., Chen,B.H., Wang,Y.W. and Teng,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.9; trycycler v. 0.5.3; medaka v. 1.6.0;
            homopolish v. 0.3.3; polca v. 4.0.5; polypolish v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 331x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/23/2023 02:21:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,826
            Genes (coding)                    :: 4,707
            CDSs (with protein)               :: 4,707
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 53 of 119
            Pseudo Genes (incomplete)         :: 82 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 31 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100714.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Salmonella enterica subsp. enterica serovar
                     Blockley"
                     /mol_type="genomic DNA"
                     /strain="R18.0186"
                     /serovar="Blockley"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:57741"
                     /plasmid="pR18.0186_4.6k"
                     /geo_loc_name="Taiwan: Taichung City"
                     /collection_date="2018"
                     /collected_by="Centers of Disease Control, Taiwan"
                     /subtype="ST52"
     gene            join(4541..4593,1..97)
                     /locus_tag="NL733_RS24680"
                     /old_locus_tag="NL733_24680"
     CDS             join(4541..4593,1..97)
                     /locus_tag="NL733_RS24680"
                     /old_locus_tag="NL733_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     gene            289..1128
                     /locus_tag="NL733_RS24685"
                     /old_locus_tag="NL733_24685"
     CDS             289..1128
                     /locus_tag="NL733_RS24685"
                     /old_locus_tag="NL733_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(1816..2049)
                     /locus_tag="NL733_RS24690"
                     /old_locus_tag="NL733_24690"
     CDS             complement(1816..2049)
                     /locus_tag="NL733_RS24690"
                     /old_locus_tag="NL733_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_049264670.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFVTNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2056..2616)
                     /locus_tag="NL733_RS24695"
                     /old_locus_tag="NL733_24695"
     CDS             complement(2056..2616)
                     /locus_tag="NL733_RS24695"
                     /old_locus_tag="NL733_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2651..3085)
                     /locus_tag="NL733_RS24700"
                     /old_locus_tag="NL733_24700"
                     /pseudo=""
     CDS             complement(<2651..3085)
                     /locus_tag="NL733_RS24700"
                     /old_locus_tag="NL733_24700"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3294..3617)
                     /locus_tag="NL733_RS24705"
                     /old_locus_tag="NL733_24705"
     CDS             complement(3294..3617)
                     /locus_tag="NL733_RS24705"
                     /old_locus_tag="NL733_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3680..3871
                     /locus_tag="NL733_RS24710"
                     /old_locus_tag="NL733_24710"
     CDS             3680..3871
                     /locus_tag="NL733_RS24710"
                     /old_locus_tag="NL733_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3960..4044)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          4055..4593
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.715"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(1719..3304)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttgagtcc aacccggaaa gacacgtaaa aacgccactg gcagcagcca ttggtaaccg
       61 tttagaggag ttagtcttga agttatgcgc cggttaaggc taaactgaaa ggacagattt
      121 ggtggctgtg ctcctccaag ccagttacct tggttcaaag agttgctgct ctgagaacct
      181 tcgaaaagcc gccttgctgg gcggtttttt cgtttacgga caagactacc ttcaggatcg
      241 ttagatctca agacaatcat gcatagtgag catacacagg agccgaactt gcttacactg
      301 ttcatctttt ttgttttatt aatagctgca tgtttctttt gttttgctcc accacgaaga
      361 ggatatgaca gaaatgagat cattccatac aaaataaaac tatcaataaa taaatatcgc
      421 ctttacatat actcatcggg aaaggtaaga caataccttt tatttttagt tattttatca
      481 ctgtactact ccatagcgga accatttaag tctgagttaa taaaaaacat atcatattca
      541 ctgatggctg catttatttt tgatacaggg cttaatttta gcaaggagaa tataactaaa
      601 ggagtgattt caacaaggtg gcataatgac ctatattcat catttgagag aatgaaagca
      661 attaataaaa tttactaccc atccaacaaa gagataaaca cagaaggtct atccaaagcc
      721 atcacatcat ctcttttcaa tgatgacgcg aactcgttcg caaagagaga ttttcgcttg
      781 atgtgggact tatcttctga aaaatatctt tcctataaag aaataataat aagaaaaggc
      841 gataagcttg acgctgtatg cttgagattt ataaatgatg actacaagtt tttagttaat
      901 ttcaacagag atgaagaggt tttcaaatac tttccatcta taatgcaacc ttcactcaaa
      961 acatatagag cgttatcaag actagtaaac tcaatcaaag atccttctag atttaagttc
     1021 acgacagagt cattagaaat ggagttacta gaatacttag agttaagaaa tgaattattc
     1081 aatgacatag aagaagtaat gggtagctat gctcagaggg caccatagtt catgatagcc
     1141 ctcgatagta cttacttata tttttagaga ggtttaagtg taagtagagg gagtaacacc
     1201 tggtttttca gcatccactc caatgaatgc tgacaccgat acgcgataat tctctttatc
     1261 gtagcaaaat catcctccag attgcggttg agaataggtt catggtgcgc aatacgattg
     1321 cgcagttttc tcacgggact tgttagcagt aatgcttccc cccggggaca aacgacagag
     1381 atatacaaca aaggcccgca attaaaatac tccgtattta ccataacgtc gcattgcgcg
     1441 taccgcttaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaaca gcaacaaacc
     1501 acccgaaaaa ctgccgcgat cgcgcctggt aaattttaac ctcatgcata gttatgcgcg
     1561 aaactgaatg acgatcagtt tcgcacgttc cagccgtcga acggggcaaa atggggaggt
     1621 ttggatattg ttaatgctaa tgtacgtaca ttaacattgg cgccatccgg gtagggcgtg
     1681 ccacaaactg gctcgatttc agggggtacc ccggtcacat ccccattgac gggccacgcc
     1741 agatccgcgc ttcgggttcc tgctcttcat gccagcgccg ggcaagctcc tgcgcctgac
     1801 gctcctgttc atcccttatc tgttgttcgt gacaaataac cgactcaagc ggtcgacttg
     1861 tccggctaat agctgcacct gctgactcaa gttcacgaca cgctcgttca gtatcgcgtt
     1921 gttctgttgc gtaaccgcgt gcatcttctg caattctgcg aaggcgctct cccactcgtt
     1981 cagccgccgc atatagtcct gttgcaaatg ctctaaggcg ttcagcaaat gcccttccag
     2041 ttccgtcata ctcgtttact ccctgaccag cctcacggcg ttcttgtcgc ccaccgtcca
     2101 ggtgttttcc cctttcaccc cgtccggcag caccagaaat ttcccgttct cgccattgac
     2161 gaacgtcacc ccccaggttt ttgcctcgag tttcgccagc gtgttttcct gctctctgat
     2221 ggtcaagata tttttcgcaa tcctgctttc ctgccactga atcagccccc agcaccccag
     2281 gaaaatgccg cccgtgacgc tgagcgcggt cagcgccgga aacagtaccc gccctttgat
     2341 cacctgccag agcaggtttt tctgctgccg ggacagttcg gcctctgtgg tgctgagctg
     2401 cgtgctcacg gtggtgctca gcgtatccag ttgctgtttc acggatgttg tatgttcgct
     2461 gatagcgtct ctgattttct gcctgtttaa gctcagtgcc ttgtctacag actcttctag
     2521 cttcctgaac tcgctgttca gcatgttctc ggtagaagcg gctcgctctt tcagtttctt
     2581 ctcgaagtcc gttcccattt gtaaaagatt gctcatacag cgcccccttt agcctgagat
     2641 tccgcccccc ctccgggtcg gcaatactga tactgcttct ggttgtcctc accacctcaa
     2701 aacctgccgc tgtgagcgcc tcagtgacat cctgacgcgt ttttattgct ccggcacggt
     2761 aaagcgcctc aagaccccgt gtaatcgctt ctgcggcctc ctgtttcgct tttggcagat
     2821 tattcggtgt gacaagcgtc cgtctgttct ccggtgcgtt cgggtcacgc agcccgtaat
     2881 gatgattaac cagcgtctgc caggcattta tcctcggcct gtccgcacgg tcgtagtacg
     2941 gctggaggcg cttaccgctc tgtagttcca tgttcggaat gacaaaattc agttcgagcc
     3001 gccccttatc ctggtgttcc acccagagaa tgctgtactg gtctttttcg agaccgggca
     3061 taagaacacg ctcaaagctc gtcatcactc tttcgcgttc acccggcggt aactccttct
     3121 ccgcgaacga cagaacgcct gacgtgtatt tcttcgaaaa tggcgtggca tcaatgagtt
     3181 cccggatttc ttccgggtta ccccggagca ccgttgcgcc ttcgcggtta cgctcccgcc
     3241 ccagcaggta atcgaccggg ccacttccac cgcctttccc cctggcatga aatttaacta
     3301 tcatcccgtc ccccctgttc cctgacagcc tgccgcaact ggcgcaattc accctcgatg
     3361 gccatcagag cggccaccac atgaacccgg tcaatcgaag accagtggcc gctgttcacc
     3421 ttccgggctg tctggttcag attgttcccg atggcagcca gatgccgcag caatggcggc
     3481 gatagtgtcg gcagttttcc tgtgcgcgca accggctccc ccagacagac ccgccgcatc
     3541 cattctgcca gtcgtttacc ctcgcagcgt tccagtaacc gggcgtgctc ctcatcgcta
     3601 acccgtatcg tgagcatctt ctcgcgtttc atcggtatca ttccccctaa aaaatcccct
     3661 attaaggagc cttaagacca tgaccaaaca ggaaaaaaca gcccttaaca tggcccgctt
     3721 tatcagaagc cagacgctga ccctgctgga aaaactcaac gagctggacg cggacgaaca
     3781 ggcagacatc tgcgaatcac ttcacgacca cgccgacgaa ctctaccgta gttgcctcgc
     3841 acgcttcggc gatgacggtg aaagcaactg aaacatgcag ctcccggaga cgttcacagc
     3901 ttgcctgtga gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggttttag
     3961 cgggtgtcgg ggcgcagccc tgacccagtc acgtagcgat agcggagtgt atactggctt
     4021 aactatgtgg catcagtgcg gattataaga aaagtgcacc atatgcggtg tgaaataccg
     4081 cacagatgcg taaggagaaa atgccgcatc aggcgctctt ccgcttcctc gctcactgac
     4141 tcgctacgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa gacggtgatg
     4201 cggttatcca cagaatcagg ggataacgcc aaaagaaaca tgtgagcaaa aggccaggaa
     4261 caggaagaag gccgcgccgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4321 caaaaacaga tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     4501 tctcggcccg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4561 gcccgaccgc tgcgccttat ccggtaacta tcg
//
