LOCUS       NZ_CP100713             4657 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Blockley strain R18.0186
            plasmid pR18.0186_4.7k, complete sequence.
ACCESSION   NZ_CP100713
VERSION     NZ_CP100713.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29431224
            Assembly: GCF_024266465.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Blockley
  ORGANISM  Salmonella enterica subsp. enterica serovar Blockley
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Chiou,C.S., Chen,B.H., Wang,Y.W. and Teng,R.H.
  TITLE     28 Salmonella enterica strains that have azithromycin resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Chiou,C.S., Chen,B.H., Wang,Y.W. and Teng,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.9; trycycler v. 0.5.3; medaka v. 1.6.0;
            homopolish v. 0.3.3; polca v. 4.0.5; polypolish v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 331x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/23/2023 02:21:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,826
            Genes (coding)                    :: 4,707
            CDSs (with protein)               :: 4,707
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 53 of 119
            Pseudo Genes (incomplete)         :: 82 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 31 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100713.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Salmonella enterica subsp. enterica serovar
                     Blockley"
                     /mol_type="genomic DNA"
                     /strain="R18.0186"
                     /serovar="Blockley"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:57741"
                     /plasmid="pR18.0186_4.7k"
                     /geo_loc_name="Taiwan: Taichung City"
                     /collection_date="2018"
                     /collected_by="Centers of Disease Control, Taiwan"
                     /subtype="ST52"
     gene            complement(join(4558..4657,1..146))
                     /locus_tag="NL733_RS24650"
                     /old_locus_tag="NL733_24650"
     CDS             complement(join(4558..4657,1..146))
                     /locus_tag="NL733_RS24650"
                     /old_locus_tag="NL733_24650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058818351.1"
                     /translation="MQPQPAVHDGLPGINARTVGAVVIRLETLAGQQLRVRDQEVQLQP
                     PLILVLNPQHAVLVFIESGHQNPFKARHQLFALPGR"
                     /name="hypothetical protein"
     gene            397..882
                     /locus_tag="NL733_RS24655"
                     /old_locus_tag="NL733_24655"
     CDS             397..882
                     /locus_tag="NL733_RS24655"
                     /old_locus_tag="NL733_24655"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140031164.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSAAMSQRTKGMLRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTTIREQKHTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            886..1101
                     /locus_tag="NL733_RS24660"
                     /old_locus_tag="NL733_24660"
     CDS             886..1101
                     /locus_tag="NL733_RS24660"
                     /old_locus_tag="NL733_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1300..1851)
                     /locus_tag="NL733_RS24665"
                     /old_locus_tag="NL733_24665"
     CDS             complement(1300..1851)
                     /locus_tag="NL733_RS24665"
                     /old_locus_tag="NL733_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            2327..2806
                     /locus_tag="NL733_RS24670"
                     /old_locus_tag="NL733_24670"
     CDS             2327..2806
                     /locus_tag="NL733_RS24670"
                     /old_locus_tag="NL733_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001050742.1"
                     /translation="MNLLLPRDIVEAVLNDKKTKNARVAKCDGSEFFLELPSMNADFPA
                     GKIILKLGDSGFYNKRTKSLEGAYGLRHIWDKHRVEIGATSAEDIVIFLESILLAGAEV
                     LIDPKKGQNKAIVVESGTGMMILELKKPNGEDPYYSIITAYDRKSHPGTKLHTLI"
                     /name="hypothetical protein"
     gene            4053..4376
                     /locus_tag="NL733_RS24675"
                     /old_locus_tag="NL733_24675"
     CDS             4053..4376
                     /locus_tag="NL733_RS24675"
                     /old_locus_tag="NL733_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001347450.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3766..3949
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.043"
                     /coverage="86.0"
                     /evalue="2.7300000000000002e-42"
     Typing          complement(3307..3783)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.083"
                     /coverage="92.0"
                     /evalue="3.51e-174"
     Typing          7..1205
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.5"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4054..4373
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.43e-65"
     Typing          4517..4656
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="80.0"
                     /evalue="2.13e-25"
ORIGIN
        1 aggataaggg gcggctggag ctgaacttcc tgatcccgaa cacggagctg ctgaccggca
       61 agcgtctcca gccgtattac gaccgcgccg accgtccgcg cattgatgcc tggcagacca
      121 tcgtgaacgg caggctgggg ctgcatgacc cgaacgcgcc tgaaaaccgg cgggcgctgg
      181 tcacgccgtc cgcgctgccg gaaacgaagc aggaagccgc tcaggcaatt acacggggtt
      241 tactggcctt agcctcgtcc ggggagctga aaacccgcca ggacgtcact gaggcgctgg
      301 aaagcgcagg ttttgaggtg gtacgcacca caaaaagcag catcagcatt gccgacccgg
      361 acggggggcg aaacatccga ctgaagggag ccatctatga acagtctttt aacgctggcg
      421 aaggacttag agcagaaatc gaaagcgcag cagcagagta ccggcgagat gctgaaagcc
      481 gcattcagcg agcacgagaa gtctgtcaga gcggaactga gcgaaagcga gaagagaatc
      541 agcgccgcca tcctcgacca cgacaggaag ctgtccgctg ccatgagcca gcgcacgaaa
      601 ggtatgctgc ggatggtcag ccagacgtgg ctgaccatcg tcttggtgtc tacactgctg
      661 acagcatcgg gcgcaagcat tctgtggtgg caggggcagc agatactcga caattacacg
      721 accatccggg agcagaagca cacgcaggcc atgctgtccg agaggaacag cggtgtgcag
      781 ctctcgacct gcggagagca gagacgccgc tgcgtgaggg tgaacccgga agcgggacgg
      841 ttcggagagg attcgagctg gatgatactg gcggggaaat agcacatgac ggagctggaa
      901 aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag gctggacgag
      961 tgggagaacg ccttcgcgga atggcggacg atgtctggtc ttatgcaacg ggagaacgcg
     1021 gcgctgagcg agcgcgtcac gggcttgagc aggcaggtgc agagtttgag cgagcagctg
     1081 cgccggttgt cacaaggctg aacgtgatag aggcacatcg tgagcaggaa cgggcggcgc
     1141 agcatcagaa agcgctggag ctggaacgat ctcagcgaca gcgaacgtac aacggtccgt
     1201 cactttgaat ctgtgcgccg gaaaagcatg ggcttgctgt ttctggcgta caggttcagt
     1261 tctagaacat gaggctgcta tcttgttgtt cacgcttcat cagatcttga tgcttattgt
     1321 tatggctgag ctatcaatcc ctgttgcgtt gctaatatcc tttttggctc tttcaattgc
     1381 ctcgttaagg gagagttttt ctgttttctc ttcagccaga ccaggaatca ctgtattagg
     1441 aaccgatggg gatgaaggat ggaatttctt ccattccagt tgttccggtg ttaccccaat
     1501 acgtaaaaaa gcttcctgtg cgctcaggta agcaaccgga tagcgctgac catcggttag
     1561 cattttccat gcttttggcg tgtgaatttc tgcgtattca tcaaaggtta tgacgttcct
     1621 gtcgtcgtaa tcagggcttt taaaacaccc ggtaattttg gcgatcagga atgctgtgcc
     1681 gtgttccagg tcttttactg cccacgtttc ccttctgttt cttactgcta tgaggtaatc
     1741 ggcatcgttg agcctgtctg ttctggcatg ccagtagcct gatcccccct cagttctcat
     1801 tatgttcaaa tccttcccgg taaaaaccac aacagctttc atatctttca tatagaaacc
     1861 cttaataggt gagtgaattt cattttttat ggaatctata cgataaaaat cgtgttgtaa
     1921 atgttttttt atgcgataaa atacagtcct cctacctctt ttattgttag tggtaaggct
     1981 ttctgtttgg ttttttgata aaccagttaa aacaggggct tggacgagga tatacattga
     2041 aagcatgagc aattcacgtg gctgcatggc tatgcagcca cgtgaaattt atcaggcgca
     2101 gaatgcgctg tttagcgggg agtttactgc cggttttacc gggctaacgc cgctttcacg
     2161 ctgaacccgt ccggagcggt gcgggcaagg aatgttatgg taaatacaac tggttttttc
     2221 tatcaagatt tactggaaat tgctcattac gtcagtgtta gaatcagccc ttcaaaaagg
     2281 gatttaacgc ctaaacataa tagtgaaggc tatagaggtg tagtttgtga acttgttact
     2341 tccacgcgat atagttgaag ctgttcttaa tgataagaaa acaaaaaatg cacgagtggc
     2401 taagtgtgat ggaagtgaat tttttctaga actgccaagc atgaatgcag actttcccgc
     2461 agggaaaatc atcctcaagc taggagattc cggtttttac aacaaaagga caaaaagtct
     2521 cgagggagcc tatggcctta ggcatatatg ggacaagcat cgtgtcgaga taggagctac
     2581 ttccgctgag gatattgtca tctttttgga gagcatatta ttagctgggg cagaagtgct
     2641 catagatcct aaaaaaggcc aaaacaaagc gattgttgta gagtcaggaa cggggatgat
     2701 gatactagaa ttgaaaaaac caaacggcga agacccgtac tacagcatca ttactgctta
     2761 tgataggaag agccatccag gtacaaaact tcatacccta atatgaaaaa agccggctaa
     2821 atagccgact tcttcgtaat agtgttgttc gtggcattcc cttagcattt cggttcggga
     2881 caggtctttg accttaacaa cgcattgtcg aaatctggtt tccgacaagt agcctacaaa
     2941 tatgtaacca atccagagtt caggttacat accaagccct tctacacata cagaatacaa
     3001 ctctatcact gctcatcttt ttgcaaaaag tgctattttg gtgttcagtg gaaaccaaaa
     3061 cgaagccaaa tcactggtaa acggtcagat ctccttgtga tcgttttact ctcgtcgtaa
     3121 tctctctccc tgtaaacgaa aaaaccaccc ggcaaggtgg tttttcgaag gttcagtaaa
     3181 ccggcaagtt tctgaaccgt ggtaacagtc ttgtgcgaga catgtcacca aatactgtcc
     3241 ttccagtgta gcctcactaa ggcctccact tcaagaactc tcgatacatc tctcgcacat
     3301 cctgcttacc agtggccgct gccagtggcg ttaagtcgtg tcttttcggg ttggactcaa
     3361 gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
     3421 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc
     3481 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     3541 gagccaccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     3601 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcaac
     3661 gccggatcct tttccccttc cttcttgatt tgagcgctat tcatctcgtc attcttgagc
     3721 aggtaacgcc cgccgcagtc tcacggcagg agcgtagcga ccgagtgagc gaggaggcgt
     3781 catttcatcg tctctagcat ttcgcactta cgccccacag ctgaattttt tacctgccac
     3841 atgaagcact tccctgaatt tttacttcga tttaccatac taagccagta tacactccgc
     3901 tagcgctacg tgactggttc agggctgcgc cccgaaaccc gctaaaacca ctgacgcgcc
     3961 tgcggcttgt ccaacaccgc gccgaccggg aaaagatttc ccgcccgtcc cggcgttatc
     4021 aggaggccgg attggcagac aaacgcagca aaatgctcac gatgtgggtg accgaagatg
     4081 agcaccgccg tctgctggag cgctgcgacg gtaaacagct tgcagcctgg atgcgccaga
     4141 cgtgcctgga cgagaaacct gcccgcgcgg gcaagcttcc ctcgctctcg ccggcgctgc
     4201 ttcgtcagct tgccggcatg gggaacaacc tcaaccagat tgcccggcag gttaacgccg
     4261 gtggtggcag cgggcacgac cgcgtgcagg tcgtcgccgc gctgatggcc atagatgccg
     4321 gactcgagcg gctgcggcat gccgtgctgg aaaagggtgc agacgatgat cgttaagttt
     4381 cacccgcgag ggcgcggcgg tggcgccggt ccggtggatt atctgctggg gaaagaccga
     4441 cagcgcgacg gcgccaccgt tctgcagggg aagccggaag aagtcaggga gcttatcgac
     4501 gcctcgccct acgcaaaaaa gtacacatcc ggcgtcctgt cctttgccga aaaggattta
     4561 ccgcccgggc agcgcgaaaa gctgatggcg agctttgaac gggttctgat gcccggactc
     4621 gataaagacc agtacagcgt gctgtgggtt gagcacc
//
