LOCUS       NZ_CP100541             2096 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Escherichia coli strain LH10-b-1 plasmid pLH10-b-1-k, complete
            sequence.
ACCESSION   NZ_CP100541
VERSION     NZ_CP100541.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499312
            Assembly: GCF_024225755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,291
            CDSs (total)                      :: 5,175
            Genes (coding)                    :: 4,870
            CDSs (with protein)               :: 4,870
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 99 of 305
            Pseudo Genes (incomplete)         :: 210 of 305
            Pseudo Genes (internal stop)      :: 61 of 305
            Pseudo Genes (multiple problems)  :: 59 of 305
            Pseudo Genes (short protein)      :: 4 of 305
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LH10-b-1"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pLH10-b-1-k"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            complement(478..1467)
                     /locus_tag="NLZ08_RS26365"
                     /old_locus_tag="NLZ08_26370"
     CDS             complement(478..1467)
                     /locus_tag="NLZ08_RS26365"
                     /old_locus_tag="NLZ08_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_024136759.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKGG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          complement(395..476)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="90.0"
                     /evalue="3.4000000000000004e-27"
     Typing          561..752
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 aaaggggtgt cccctttggc acaagcgacg gagcgaaacg ccccgttttg tgtacgccgc
       61 ttatcgtgcc tgtgttttag gagaacaaaa aaatgccgtg catacagccc acacaatgac
      121 ccacataatc acccacaact caaaacacac agcccacaca atcacccaca ctttaaccca
      181 caaaaaagac cacaacccca ttgacacccc acaaaacaac ccacacaaac ggcgagtaaa
      241 tcattttgcc gttttcgtgc cgcagccgtt ccgcgcaatc gacaaactgg cagtcaggga
      301 atctcacgaa gtgcgcactt atacaaactc accttaggtt cgtttgtcgt gcggtctgcg
      361 acgctcaaaa aaccgagaaa cggcgggaat tttcgggaca ggatgtgtaa ttgtaatacc
      421 gtcacacgcg acgctatcac aattgccatc tggtcagggg tgcaccccga caccccgcta
      481 gcctgattca gttttatctt tcgtcggtga gcgacggtac tttctgacct cggttttcca
      541 ctcgaacgcg attcgtgaac cgtcatcgtc gccctcggaa atatcatcac cgatcaccat
      601 gtcggcgtca gtttcctgat cgagtttcag cacgtctttg agtgcgccgc cggtcgccac
      661 aaagcggcgc ttgtgcgtct ggcgggtcag ttcgaggaac cattcaggat cggccaccat
      721 gtcagccggc ttcgtggaat atttcagggt ttcggccacc gcaccgcgca ccagttcggc
      781 ggtcgcgcag gccagcgatt cgccgccctt cggcttacga ggtttcactg cccggacatc
      841 aacattcggg tcataatcga cccgcagaca ctcccgccag agttcaaccc agcgttcatg
      901 cctgacgtaa cttttcccat ttagcatgcc aggcggcacc atcatcaggg tgtggaagtg
      961 cgggtgtgcg ctgccgtcgc tcccgcgggt gacttccgtg gtacgtatcc agccctgcac
     1021 aggccggaac tccttgcgaa ccttcatgcg ctgaaaagct gcattcatcc ggttcagcat
     1081 ctcgcccagc tccccgatcg cacagttgcg cacggtcaga gtcaggaaca tccagcgggc
     1141 atcgggataa tccgccacga tacgcggcag ggactggtag aaacgggctt gccacatcag
     1201 ggagcgcctc cactggcaga caggacaatg ccggacacgg cagaaatgcg cctcacgcag
     1261 cctcagacgc gtttctccgg tttccttgag ggtagaccac ccgaaacgca gtaaaccacc
     1321 acagagagcc atacgcgccc cgtagcgctc atattcggct gcaagcaggt aaatccctcc
     1381 cacgtcatca gaaacgcttc tgtgagcgtc ccagggcgca tctgaggggg aatagtccgt
     1441 gaggttctgg agatcagggt ttttcatgcg caccacccga tagtgggcga tggctcatct
     1501 gacgtgttat cattgccata tcacctgttg tgttagtcgt atcaccaatt cgaattccta
     1561 caggtggtca cacccccgaa cgattgccct cgttgcgggg gtcgctttta atgcgaccac
     1621 tcccgacaga ataactccaa aaccattttc cgacaaatcc cccttcaggc cttgcggcac
     1681 aagggctgga ggcgaatttg aatcctagag ttatccctta agtataaata caaggatcgc
     1741 gcgcgagatt cgcttgaatt ttgagcggga ttttcagtca cccaaaacac ccaaccccac
     1801 ccacttacac ccatctcatc cacagacttt caccccgatt ttgtgaacaa caaaaaaaac
     1861 cgtcttgcag gtggttggcc tcactgcgtg acaccaacca ccatccccag tgtttttccc
     1921 gtgccgtcgt caatcacaaa atcggaaccc acgccggagg cgcgggcccc cgcgattttc
     1981 tgtttgacga atcagggaac ggtgaacggt acgcggaatt ttttcgcgta ccgtgaggcg
     2041 ttggcggggt gtcggggcgc agtcctgacc aggttcgtag aaccgcacag ggttgc
//
