LOCUS       NZ_CP100538             4073 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Escherichia coli strain LH10-b-1 plasmid pLH10-b-1-H, complete
            sequence.
ACCESSION   NZ_CP100538
VERSION     NZ_CP100538.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499312
            Assembly: GCF_024225755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,291
            CDSs (total)                      :: 5,175
            Genes (coding)                    :: 4,870
            CDSs (with protein)               :: 4,870
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 99 of 305
            Pseudo Genes (incomplete)         :: 210 of 305
            Pseudo Genes (internal stop)      :: 61 of 305
            Pseudo Genes (multiple problems)  :: 59 of 305
            Pseudo Genes (short protein)      :: 4 of 305
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100538.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LH10-b-1"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pLH10-b-1-H"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            join(4042..4073,1..223)
                     /gene="mobC"
                     /locus_tag="NLZ08_RS26310"
                     /old_locus_tag="NLZ08_26315"
     CDS             join(4042..4073,1..223)
                     /gene="mobC"
                     /locus_tag="NLZ08_RS26310"
                     /old_locus_tag="NLZ08_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            757..1458
                     /locus_tag="NLZ08_RS26315"
                     /old_locus_tag="NLZ08_26320"
     CDS             757..1458
                     /locus_tag="NLZ08_RS26315"
                     /old_locus_tag="NLZ08_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1716..3863)
                     /gene="mobQ"
                     /locus_tag="NLZ08_RS26320"
                     /old_locus_tag="NLZ08_26325"
     CDS             complement(1716..3863)
                     /gene="mobQ"
                     /locus_tag="NLZ08_RS26320"
                     /old_locus_tag="NLZ08_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_112021495.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRGWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(3506..3864)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3865..4041
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          758..1458
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1720..3950)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.856"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgctgaggcg agattacagc gcctgaaggc gaagagccgg agcctggaca cagcgcagaa
       61 ggttgttgtg ggtgctgcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg
      121 tgcctggtta ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat
      181 actgccgctg attaacgagc tggaggcgct gccagaacag tgattgtatc gtccgctcgc
      241 cgcagcccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatcagt
      301 cgccaggttg agcacatacg cacggttttt cggcaggtga tatgcgctga acccgcatga
      361 atactgggtt tccacagggt tggagggggt gtattcgaaa cttgcaattc tgctttattg
      421 caacgcattg tttttattgg ttttttgttt tcagcacccc aagttatcca caggctcgcc
      481 gttaagtttt taagatctta tttttagatc ttttttttta aaaccttttt aggtcttgta
      541 acaccatgat tttaaagatg ttctggcaag ataagtggag cttttggggg tgaaaggtgg
      601 agcttttggg ggtgaaaggt ggagcttttg ggggtgaaag gtggagcttt tgggggtgta
      661 atccacagaa aaagggggtt gcaaaaaaaa aacttccacc catatgataa gtggcgtaca
      721 gtaaaaaaac gccaccaata gcaacgagca agcgaaatgt cagatctgat agcttacaaa
      781 tcaaacgccc ttatcgaggc aagctacaag ctcactttgc aggaacagcg cttcctgctg
      841 gtctgtatca gccgcctgaa atccggagaa gattcccctt cgccggatga acaaaaaacc
      901 atgaccatca cggctgcaga gtttcatgat gcctttcctg acatgggacg gcagcacgct
      961 gaagctaggc tcaaggaagc gatagatcgc ctctgggaca ggtcagtaat catcaagaac
     1021 gaggaaaaac gagaagagtt ccgctgggtg cagtaccggg cgcagtattt caaaggtgag
     1081 gcgaaagtag atatcacctt ctccgatgcc atcatgccgt atctgacaca gctcaagggg
     1141 cagtttacgc gggtagtcgt gaagaacgtc tccagccttt ccagcaccta cagcatccgc
     1201 atttacgagc tgctccagca gttccgcagc accggtgaca gaacgatagc tatcgatgat
     1261 ttccgaacca tgcttgccgt tgaagataaa tatcaacaat tcaaagactt gaataagatg
     1321 ataatcaagc cagccatcgc cgaactgaac gaaaaaagcg atctggttgt aactgttgag
     1381 acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc gcttcaagga agacaaacag
     1441 atcaagatga cgatctaaaa tcgattttaa ggcacctaaa acgctccctg ctgcgttttt
     1501 tttgtgttag tagtgcgaag gtactattga agtgaaaaat tgcgtcaaaa acgctctgat
     1561 gcagcgaaaa aagacccccc aatttttggg ggcggaaaaa caaaactatg cttgcgttat
     1621 gtgagtgtgc ttcttgcatt catccctgtc gatgaacatc gaagaacatc gacgaacata
     1681 acacgatgtt aaacaaaaca aaatattgtt cacattcact gtgaccaacc gcgatcatgt
     1741 ctcctctcgg tggtgtgtgt tcgtgcatct tcctcgcgct gccattcctc ccaggtacga
     1801 ttaaaaccca gcgagcggaa cagatgatcc ggctgttttt ctggtgtgtt gacggcagca
     1861 aaaagcggtg cctcacgggt tttcagatga aactgcatca gttctggtgc aggtgatatg
     1921 tggctgtctg catcgtgatt tacggcaaca ccataccctg cataccgtaa ccccttatcg
     1981 gtcagcgcac tgatgaccag atgcctgtta tcggctgagg gaacggcaaa cgcgggatac
     2041 agcgtaccat taagccggat aacaccgccc agagagtcat ccacgcccat gaccagcaaa
     2101 agctgaagct ggcgggtttc ccggtcgaca gaactcatga ccggcatcag cgtcggtggc
     2161 agacgtccct gcataaaatc caccttcctt gccgctggtg atggcatggc tggctggcgg
     2221 tcttctccaa gacagaaaga ctccggccat gtcttcggct ggcggagatt gcgggtatgt
     2281 tgcggccatg tctggcaaac ctgcgtcagc agttgctcaa aggttgcaga ggaataaaca
     2341 cgttgcagtg gcggacgttg gccgatttcg cttcggtcac agatataacc actcctgtca
     2401 cgcattaaat caacactctc aaagattctg gtgcggtatt tctccagctc gttcccccag
     2461 agcgtatatt ctccgttccg gttgtgaagc agaaggtaac aggtcatttc cccgttacca
     2521 ggcatgggag cctcgccata ccccgtaacg actgctctca gcacccctga atccataacg
     2581 gcattgtcat ttttcctgag ctgctccgcg cgactacgtt cagtatcttc ctgttccttc
     2641 cggcgcaatt cctcttcacg tttaagcaac tgggctctga gagctgcaag gccggattcc
     2701 ccccactgct gccactgttt ttcaaatgcc atctgactcg cgggaatgtc ctgtttctcc
     2761 accttccggt aatgctgtcg taatttttcc atttgctggc tcagagactg gatttttccc
     2821 tgctcactac ggtattccgg tgccgcccat tgcatcaggg gaatacggga aaaaccggac
     2881 gaaaacggcg atgatttttt caggctggca gcatggctca tcgcttcctt tcgttcggtg
     2941 ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga catggcatta
     3001 ctggtgagtt tccatgccga gttactgtcc ttcggcaacg cttcgataat gtcacgtagt
     3061 gtgtacgaag ttgcgttgtc aggaagcccg atattcagaa ggtcagccac acggcgggca
     3121 ggctctgtca gccctccgtt cctgagttcg gtgcgggaaa tctgtatttc caggttaagc
     3181 ctgttaatca gacgattctg ttcgccgcgt tctgtttcga taccgcgtgt ttccatcgca
     3241 caggcagact ttccgaggtg aataccaggc gttttctcaa gcccctgtcg ctcgtatgaa
     3301 cgatggtcta tctcttcctg ataaccggcg ttcgccagtg ccctgtttgc atggtcagcc
     3361 catgatgccc gccacgtctc ggcatgtgtc cggtcgttcc agcccctgac ctttccgctg
     3421 aacccagcag gtccgatagc cctcgtggtc agcatgatgt gcgcatgggg atttgtttta
     3481 tccatgtgat ggaacgccac atcagcaatc atgccctgac tgacaaagtt ttctctgaca
     3541 aaggcgagaa cggtttcccg tgcggcatcc tgcggcagtt cgtgggggat ggcgagttca
     3601 atttcccgcg caagttgtga gttcttccgg cgttcggcct tctcaacggc attccagagt
     3661 tcggcacggt tcgtacacca ggcaggggcg gtggcggggg tcagaatcac agcctcagcc
     3721 acaccgcttt tgcgggtgta gtcgtgagtc agtccggtac gctcatcaag catcagtgaa
     3781 ccggaacgat aggcagcgga agctacggcg ctgtagccgc tgcttctgga gataattttc
     3841 atgctgagat gataaatcgc catttctgcc gtaatgcctt tttgtagttt tctttcagcc
     3901 ccgcagggcg cacacaatcg aaacggagtg gagattgtat aagtgcgcac ttcgtgattt
     3961 gccgtgcatt atgccagctc agtttttaaa ctaccagtga cgcgctaaaa tgcgattttt
     4021 catcgcgcac ggagttacat catgagcaga acgctcgaac agaagattgc tga
//
