LOCUS       NZ_CP100537             5594 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Escherichia coli strain LH10-b-1 plasmid pLH10-b-1-G, complete
            sequence.
ACCESSION   NZ_CP100537
VERSION     NZ_CP100537.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499312
            Assembly: GCF_024225755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,291
            CDSs (total)                      :: 5,175
            Genes (coding)                    :: 4,870
            CDSs (with protein)               :: 4,870
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 99 of 305
            Pseudo Genes (incomplete)         :: 210 of 305
            Pseudo Genes (internal stop)      :: 61 of 305
            Pseudo Genes (multiple problems)  :: 59 of 305
            Pseudo Genes (short protein)      :: 4 of 305
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100537.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LH10-b-1"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pLH10-b-1-G"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            join(5563..5594,1..259)
                     /locus_tag="NLZ08_RS26295"
                     /old_locus_tag="NLZ08_26300"
     CDS             join(5563..5594,1..259)
                     /locus_tag="NLZ08_RS26295"
                     /old_locus_tag="NLZ08_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029490380.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            791..1492
                     /locus_tag="NLZ08_RS26300"
                     /old_locus_tag="NLZ08_26305"
     CDS             791..1492
                     /locus_tag="NLZ08_RS26300"
                     /old_locus_tag="NLZ08_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3225..5384)
                     /gene="mobQ"
                     /locus_tag="NLZ08_RS26305"
                     /old_locus_tag="NLZ08_26310"
     CDS             complement(3225..5384)
                     /gene="mobQ"
                     /locus_tag="NLZ08_RS26305"
                     /old_locus_tag="NLZ08_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_096990866.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELQQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNCGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFETQWQQWGASGLAALREQL
                     RKREEELRRKEQEETERRRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFECVDLMRDRSGYICDRSEIVQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAIPSPAARKVDFMQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNYDADSQISPAPELMKFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRRDEQHRKQERYPGNHRGMSM"
                     /name="mobQ"
     Typing          complement(5027..5385)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
     Typing          5386..5562
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="1.22e-84"
     Typing          792..1492
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.316"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3232..5471)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.315"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgctgaggcg agattacagc gcctgaaggc gaagagccgg agcctggaca cagcgcagaa
       61 ggttgttgta ggtgcagcat tgctggcaaa ggtcagaaag ccggaggaag tgcagttgcg
      121 agcctggtta ctccagttcc tgaaggcaga ggtaacacgg caggctgatg tgagcaggat
      181 acagccgttg attgacgaac tgaacgcgtt gccaaaacca gtccccaaag aagtgtcaga
      241 aaacgaccaa caggcgtgaa tgcactgtcc gctcgccgca gccccagcga ccgagcgtta
      301 gcgagcgagt gggcgaggaa gcggaagtga accgtagcca ggttgagcac atacgcacgg
      361 ttttttgaca ggtgatatgc gcttaacccg catgaatact ggatttccac agggttgagc
      421 gggagtttta ctaaagtccg cgaacctgtt ttttgtaaca tgttgttttt gctttttttt
      481 ccttttacgc cccccaagtt ttccacaggc tcgctgttaa gtttttagat cttgttttta
      541 agatcttgtt tttaatactt gttttagggt cgaattttct tatggaattc aatagtttag
      601 aagactatag gtaatataat ttaaggtaaa ggcaatatga tttaaggtgg ataggcaata
      661 tgatttaagg taaaggcaat atgatttaag gtaatatcca caggaaaagg gatattaaaa
      721 ttaaatcata ttgcctttac ctaaaggaat gtttatattt tactcattgc cttaggttga
      781 gggatttaca atgtcggatc tgatcgccta caaatccaat gcactggttg aggccagcta
      841 taaattgact cttcaggaac agcgattctt gttactttgc attggtcgat tgaagtcggg
      901 ttctgatgca gaatccactg aattgcaaaa gaccatgact gttaccgccg aagagtattt
      961 cgactcattc cctgatatgg ggcggaaaaa tgccgaggtt cagctacagg aagcaattga
     1021 tcgtttgtgg gacaggtcga tcatccttaa ggacgatgag aagcgcgagg agttccgctg
     1081 gatccagtat cgggcacagt acgctaaagg tgagggcaaa gcccaaatca ctttttcaga
     1141 tgcggtcatg ccttatctga cacagttgaa ggggcaattt actagagttg taattaagaa
     1201 tatctccaat ttatcaagaa gttacagcat aagaatatac gaacttctcc agcaattccg
     1261 cagcacaggg gaacgtatta tcgccctcga tgattttcgc tcaatgcttg atatagagca
     1321 taaatatcaa acatataagt ctctgaacca gcaattatta agaccttgcg ttgacgaact
     1381 gaatcaaaaa agcgacttgg ctgtaacgct tgagacgatc aagaaggggc gcacggttgt
     1441 agccctgcat tttcgcttca aggaagacaa acagatcaag atgacgatct aaaatcgatt
     1501 ttaaggcatc taaaacgcca tctggttcgt ttttttggtg tcaggtatgc gatggtacta
     1561 ccaagcaaat aaattgcgtc aaaatcgatt ttagagagcg gtatggcaaa ccatgtcgaa
     1621 cattacttgt tttacttgac gtgagagatg cgttaatgcc gccagcagca gtcccccatc
     1681 gaagtgactc atgcgtgact aaagagcaag cctgagcaac gtaggaatct ccaaccttca
     1741 ggacgggatg gatatcaagc accagggcac acttgaaaac aaaacctcgc ctctggcaaa
     1801 gcgcgtcctt tcacgatgac gtagaggtat ttgaatatct gtaggagttc atcacgctat
     1861 tttaacgctt aaaaagcgcg tttctgattg tgtaactaaa cacaggtagt aattagtagg
     1921 tgtggggggg gcagaaacgc gcaattaata tttatcgtga atttgatttt atgtctagta
     1981 tcgagaaaca gaatataaaa agattatctt tggaaatata gaaaaaaaat aaaataataa
     2041 actaaaaaca atcaaaaaat aataaatatt acactgcaat atggcaacaa tatattcatt
     2101 catatagtga tataagctat ggttttcaaa attttccttg agctgaagaa cggagtttta
     2161 tggcaaacga ggtaatcttg cacaggaaaa ataggttgta tctatgtcat ggttttgtta
     2221 tttttttttt tttttgctca gttattgatg gtcaatatgt aaaataaatc aggtacttcc
     2281 ttacacttac cctcattcat tgagggtttt ttttatccat atctaagttg acatcctcca
     2341 tgccctgaag gacggggttt tacagcacac cggataacta tctcgacatg gatattgata
     2401 cacgttaaaa atacgtgagg cgtttctggc agatatctga ggtctatttc gccgtgttag
     2461 ccacactgtc agaaacgcgt tcaatttctc ccgttgagct gatatgctca tcaccgcaat
     2521 cacggtagta tccgaagacg aaccgttaat gcatcttccc cccgatactg ggctttcggg
     2581 gggaagattt actcggtgcg ccgtaaaacc ccgtccttca gggcatggag gatgtcaaca
     2641 cgaatcccct atcttctttt cagtaagaaa cggtcattga aaacttatat aacactacat
     2701 ttggtgtgta ttttgcgata aaaatatccc ctatcctaat gatagggaat acaaacgatg
     2761 aattaacagt tcttaattct cacgtgaaaa gatagatttt aacgaagaca caatcagcag
     2821 tcttatatcg aatggctatc gatcctgaat cctgatgagt ggggatgttt ctgtactacc
     2881 aatgaaaagc tatccggcta gagactttac gatacagaaa cacccctctt tcttttatct
     2941 catcagatgc catagcgcaa aacaatttcg tttcacagat gtgacttaca atcaaactgt
     3001 gcaacataaa tcacaaaaca agaaggtttt gttattattt tgttgatgag ttgtttttcg
     3061 ttggttgttt ttttgaggcg tacatacgcg cacaaatgta aaactaaatt cgtgttttcg
     3121 ataattttaa ggccacaatc attatggcat tttttatcct gatcgccgtc aaaatctgtc
     3181 cttcatggca gggtttacgg cgatccggat aacaccaaag atacctacat gctcattccc
     3241 ctgtggtttc caggatatct ttcctgtttt ctgtgctgtt catcgcgccg ccattcgtcc
     3301 caggtacgat taaaacccag agagcggaac agatgatccg gctgtttttc tggtgtattg
     3361 acggcagcaa aaagcggtgc ctcacgggtt ttcagatgaa acttcataag ttccggtgca
     3421 ggtgatatct ggctgtctgc atcgtaattg acggcaacac cataccctgc ataccgtaac
     3481 cccttgtcgg tcagcgcact gatgaccaga tgcctgttat cggctgaggg aacggcaaac
     3541 gcgggataca gcgtaccatt aagccggaca acaccgccca gagagtcatc cacgcccatg
     3601 accagcaaaa gctgaagctg gcgggtttcc cggtcgacag aactcatgac cggcatcagc
     3661 gtcggtggca gacgtccctg cataaaatcc accttccttg ccgctggtga tggtatggct
     3721 ggctggcggt cttctccaag acagaaagac tccggccatg tcttcggctg gcggagattg
     3781 cgggtatatt gcggccatgt ctggcaaacc tgcgtcagaa gttgctcaaa ggtcgcagag
     3841 gaataaacac gttgcagtgg tggatgttgg acgatttcgc ttcggtcaca gatataacca
     3901 ctcctgtcac gcattaaatc aacacactca aagattctgg tgcggtattt ctccagctcg
     3961 ttcccccaga gcgtatattc tccgttccgg ttgtgaagca gaaggtaaca ggtcatttcc
     4021 ccgttaccag gcatgggggc ctcgccatac cccgtaacga ctgcgctcag tacccctgaa
     4081 tccataacgg cattgtcatt tttcctgagc tgttccgccc gtcgctccgc gcgacgacgt
     4141 tctgtttcct cctgctcctt ccggcgcaat tcctcttcac gtttacgcaa ctgctctctg
     4201 agagctgcaa ggccggatgc gccccactgc tgccactgtg tttcaaatgc cgtctgactc
     4261 gcgggaatgt cctgtttctc caccttccgg taatgctgtc gcaatttttc catttgctgg
     4321 ctcagagact ggatttttcc ctgctcacta cggtattccg gtgcagccca ttgcatcagg
     4381 ggtatacggg aaaaaccgga cgaaaacggc gatgattttt tcaggctggc agcatggctc
     4441 atcgcttcct ttcgttcggt gttcagctct tcccagcgtc tgcgcgtcgc ctgcatatct
     4501 gccatcatcg acatggcgtt actggtgagt ttccatgccg cgttactgtc cttcggcaac
     4561 gcttcgatga tgtcacgtag tgtgtacgaa gttgcgttgt caggaagcac gatattcaga
     4621 aggtcagcca cacggcgggc aggctctgtc agcccgcaat tcctgagttc agtgcgggaa
     4681 atctgtattt ccaggttaag cctgttaatc agacgattct gttcgccgcg ttctgtttcg
     4741 ataccgcgtg tttccatcgc acaggcagac tttccgaggt gaataccagg cgttttctca
     4801 agcccctgtc gctcgtatga acgatggtct atctcttcct gataaccagc gttcgccagt
     4861 gctctgtttg catggtcagc ccatgatgca cgccacgtct cggcatgtgt ccggtcgttc
     4921 cagtccctga cctttccgcc gaacccagca ggtccgacag ccctcgtggt cagcatgatg
     4981 tgcgcatggg gatttgtttt atccatgtga tggaacgcca catcagcaat catgccctga
     5041 ctgacaaaat tttctctgac aaaggcgaga acggtttccc gtgcggcatc ctgctgcagt
     5101 tcacggggga tagcgagttc aatttcccgc gcaagttgtg agttcttccg gcgttcggcc
     5161 ttctcaacgg cattccagag ttcggcacgg ttcgtacacc aggcaggggc tgtcgcgggg
     5221 gtcagaatca cagcttcagc cacaccgctt ttgcgggtgt agtcgtgagt cagtccggta
     5281 cgctcatcaa gcatcagtga accggaacga taggcagcgg aagctacggc gctgtagccg
     5341 ctgcttctgg agataatttt catgctgaga tgataaatcg ccatttctgc cgtaatgcct
     5401 ttttgtagtt ttctttcagc cccgcagggc gcacacaatc gaaacggagt gaagattgta
     5461 taagtgcgca cttcgtgatg tgccgtgcat tatgccagct cagtttttaa actaccagtg
     5521 acgcgctaaa atgcgatttt tcatcgcgca cggagttaca tcatgagcag aacgctcgaa
     5581 cagaagattg ctga
//
