LOCUS       NZ_CP100536             8038 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Escherichia coli strain LH10-b-1 plasmid pLH10-b-1-F, complete
            sequence.
ACCESSION   NZ_CP100536
VERSION     NZ_CP100536.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499312
            Assembly: GCF_024225755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,291
            CDSs (total)                      :: 5,175
            Genes (coding)                    :: 4,870
            CDSs (with protein)               :: 4,870
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 99 of 305
            Pseudo Genes (incomplete)         :: 210 of 305
            Pseudo Genes (internal stop)      :: 61 of 305
            Pseudo Genes (multiple problems)  :: 59 of 305
            Pseudo Genes (short protein)      :: 4 of 305
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100536.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LH10-b-1"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pLH10-b-1-F"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            1..915
                     /locus_tag="NLZ08_RS26250"
                     /old_locus_tag="NLZ08_26255"
     CDS             1..915
                     /locus_tag="NLZ08_RS26250"
                     /old_locus_tag="NLZ08_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_208477576.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDIDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1141..1407)
                     /locus_tag="NLZ08_RS26255"
                     /old_locus_tag="NLZ08_26260"
     CDS             complement(1141..1407)
                     /locus_tag="NLZ08_RS26255"
                     /old_locus_tag="NLZ08_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091750.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
                     /name="hypothetical protein"
     gene            1586..3805
                     /gene="mobQ"
                     /locus_tag="NLZ08_RS26260"
                     /old_locus_tag="NLZ08_26265"
     CDS             1586..3805
                     /gene="mobQ"
                     /locus_tag="NLZ08_RS26260"
                     /old_locus_tag="NLZ08_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_208477577.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCEGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKREEQRRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4037..5785
                     /gene="col"
                     /locus_tag="NLZ08_RS26265"
                     /old_locus_tag="NLZ08_26270"
     CDS             4037..5785
                     /gene="col"
                     /locus_tag="NLZ08_RS26265"
                     /old_locus_tag="NLZ08_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E9"
                     /protein_id="WP_097745325.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVAR
                     NQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQ
                     RAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGK
                     ATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKAVWEEVSKDPELSK
                     NLNPSNKSSVSKGYSPFTPKNQQVGGRKVYELHHDKPISQGGEVYDMDNIRVTTPKRHI
                     DIHRGK"
                     /name="col"
     gene            5787..6047
                     /locus_tag="NLZ08_RS26270"
                     /old_locus_tag="NLZ08_26275"
     CDS             5787..6047
                     /locus_tag="NLZ08_RS26270"
                     /old_locus_tag="NLZ08_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_012644887.1"
                     /translation="MELKHSISDYTEAEFLQLVTTICNADTSSEEELVKLVTHFEEMTE
                     HPSGSDLIYYPKEGDDDSPSGIVNTVKQWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6254..6520
                     /locus_tag="NLZ08_RS26275"
                     /old_locus_tag="NLZ08_26280"
     CDS             6254..6520
                     /locus_tag="NLZ08_RS26275"
                     /old_locus_tag="NLZ08_26280"
                     /inference="COORDINATES: protein motif:HMM:NF023528.2"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5-related ribonuclease"
                     /protein_id="WP_097054248.1"
                     /translation="MTKSDAEIVVSVTVTQSVVYVTTHAVTGRGKIQVSGLEPRNDPAT
                     VYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
                     /name="colicin E5-related ribonuclease"
     gene            6510..6839
                     /locus_tag="NLZ08_RS26280"
                     /old_locus_tag="NLZ08_26285"
     CDS             6510..6839
                     /locus_tag="NLZ08_RS26280"
                     /old_locus_tag="NLZ08_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="WP_000182536.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKKG
                     LWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTAFI
                     ITLKM"
                     /name="colicin transporter"
     gene            6937..7080
                     /locus_tag="NLZ08_RS26285"
                     /old_locus_tag="NLZ08_26290"
     CDS             6937..7080
                     /locus_tag="NLZ08_RS26285"
                     /old_locus_tag="NLZ08_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001426351.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            7432..7707
                     /locus_tag="NLZ08_RS26290"
                     /old_locus_tag="NLZ08_26295"
     CDS             7432..7707
                     /locus_tag="NLZ08_RS26290"
                     /old_locus_tag="NLZ08_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052977602.1"
                     /translation="MAYDEHSIRVMSADEIEQRFDWLRLENLAKEHRLPVDWVRRGFEA
                     CWRLGIEPDYFIDRYIFKRDVPLVPEFEVVFREIVNENRYRDRMRF"
                     /name="hypothetical protein"
     BGC             1..8038
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1409..1585)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="5.69e-78"
     Typing          complement(1586..1944)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..915
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.487"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1587..3802
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.963"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacctc atcgtcccta tcacacggat
       61 gatctcgctt tcggccttcg catctccggc aaagggcgcg ctcttcttgc gcgctacatc
      121 cagcagaacc agccacatgc gcagttctgg ctggtttttg acattgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgtaaa aaatcctgtg
      241 aacggtcatg ctcacctgct ctacgcgctc aatatcgccg tgagaaccgc gccggacagc
      301 tcggttaagg cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga gaaactttgt
      361 gctgatgtga actacagcgg actgatttgc aaaaacccgt tccacctgga atggcaggtg
      421 atggagtggc gagaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagt
      481 gcctcagcgc gccgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc attctcacag
      601 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tctccggctg aatgtcgggc tattggcaag agtattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctaca
      781 cgcggtcgca agggcggttc taagtctaag cgcggcacag tagctacatc agcacgcacg
      841 ctgaaacctt gggagaaatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtctcgtag agtagaccaa ataagcctat atcagataac agcgcctttt tggcgtcttt
      961 ttaagcagct tggtttgttg ctatttccct cgttgaatcc cgcaatggcg cggctttccg
     1021 tatgattgag gtggtagcgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc
     1081 gaggaagcgc aacggtgtcc ggtggtgcat gtggcactta cgcgccgggg tttctccgtt
     1141 ctacggtttc gcaggtggtt tgggtagctt ctccagctcg ttaatcagcg gttgtatccg
     1201 gttcacatcg gcctgtcttg tgacttcctt tcgcaggaac tggagcagga acgcacgcag
     1261 ttgcgcctct tccggcctcc gtaccctcgc cagcatggca gcccccacaa tgactttttg
     1321 cgccgtgtcc aggctccggc tcttcgcctt caggcgctgt aatctggcct cagcttcggc
     1381 aatcttctgt tcgagcgttc tgctcatgat gtaactccgt acgcgatgaa aaatcgcatt
     1441 ttatcgcgtc actggtagtt taaaaactga gctggcataa tgcacggcac atcacgaagt
     1501 gcgcacttat acaatcttca cttcgtttcg attgtgtgcg gtctgcgacg ctaaaagaaa
     1561 acggcaaaaa ggcattacgg cagaaatggc gatttatcat ctcagcatga aaatcatttc
     1621 gcgaaaaaac ggctacagtg ccgttgcttc tgctgcctac cgttccggct ctgtcatacc
     1681 cgatgaccgt accggattaa tccacgatta cacccgtaaa cgcggcgttg atgatgcggt
     1741 cattctcacc cctgcgaatg caccgtcctg gtgtggtgac cgttccgttc tctggaatgc
     1801 ggtcgagaaa gccgaacagc gccggaactc ccagctggca agggagattg aactcgccat
     1861 tccccgtgag atttcccgcg aggccgcacg ggaggccgtt ctcgcttttg tccgggaaaa
     1921 ctttgtcagt cggggcatga ttgccgatgt ggcgttccat catatggacc ggaccaaccc
     1981 ccatgcacac atcatgctga ccacgagagc tgtcggggaa acgggattcg caggaaaggt
     2041 cagggactgg aacgaccggg cactcgccga gacgtggcgc gcatcatggg ctgaccatgc
     2101 gaacagagcg cttgcgaacg ccggctacca ggaagagata gaccatcgtt catacgagcg
     2161 tcagggactg gagaaagcgc cgggccttca cctcggaaag gctgcctgtg cgatggaaaa
     2221 acggggcatg gaaacagaac gcggtgagca gaaccgcctg attaacagcc ttaacctgga
     2281 aatacaggtt tcccgcacaa ggctggcagt catccaggta caggagaacg tgcggaaacg
     2341 ggaactcagc gatgccgcac gtcgtgcagc ggaagccctt aatctgacca ttcccgccgc
     2401 gaatgcctca gcggataccc tgagggaatt cattgccaca ctgccgcagg aatgcgggaa
     2461 cgcatgggag atgacaccgg agttcgtggc gatgaacggg aaggcaaacg acatcaaacg
     2521 tgaggggaat gcgctggtga aagagcggga tattctcgaa aaagagatga ccggactcag
     2581 aaaagcccgt ccggtagcgt ccatcctgtc agagcttccc ctgatgacat gggctgaacc
     2641 ggaataccgc aaaagacaac tccgcttctg gaaactcgag aaacagattg aatctcttct
     2701 ccgcacctac agggccgtga aagaacggga cattcccgcc cgtcgtcagg cctttgaaac
     2761 gcagtggaat acgtggattt cgccgggaat ggcagagctg aaagaaaaac tgtcagcacg
     2821 ggaagcggag cggcgcaggg aggaagccga agcggaagcg cgccggaagg aacaggaata
     2881 tgacgcacgg ctgaaacacc atgataacca tcgcctgagc cgtgaaacgg cattagccgg
     2941 ggttattacg gagctggggc gtgccagaga gccgggaacg ggcaggataa cccgctacat
     3001 gatgctgagt aacagagccg gagaattcac ggtatggggt gatgagctgg cgcattaccc
     3061 ccagagtgtt catgacccgg tgaatgttta cctgtcgcca ggcggggctg tgatggtctc
     3121 ggatatacgt gagggaatgc cagaatctca tgagacgatg gcgcggcctg agcgtgtgag
     3181 aatgtattcc ggtgcgacgg tccggcatgt actggaacag atgcgccagg ggtggccctc
     3241 ttacggtttt ccggcgctgc cgcatcactg gccggataat ttttatttca gcgacgaccg
     3301 caggcccgca gcctctccgc tgccgtcagc gcaccgggtg gacgtcaccg cttatgcggc
     3361 accggagcaa ctcatgcccg ttgtcttttc gacagagcga aacagcagga cgctgaatct
     3421 gctgttgtgc gaagggccgg aggaaatgct tgtcggtttt gtgcgcctgg aggacgagct
     3481 gcgtcccgtt cttgcgcttc cgtcgccgga ttacagtcat ctgatggtaa gcatcatcac
     3541 agagaacggg atacacctgg caggttacgg tgaggccata aaccgtgatg ctgatactcc
     3601 gtacccaccg gaaccgaagc tgatgcagtt caggcttaag ggctgtcagg acacgctttt
     3661 tgctgccatc aacaaaccgg aagagatgcc ggattatctc ttccgtcaac tcggatttaa
     3721 tcagacctgg catgagtgga agcgggagga acagcgcagg caacaacaac gccgtcccgg
     3781 gcacttccgg ggcatgagta tgtgatgtcc ggggctgcac tccggacccc gccaacacat
     3841 cacgggccac aaaatttttt gtggcccgct ctgcgttttc taagtgttat ccctcctgac
     3901 ttctaaaaaa ttttccacct gaacttgaca gaaaaaacga tgacgggtac tttttgatct
     3961 gtatataaaa ccagtggttt tatgtacagt attaatcatg taattaattg ttttaacgct
     4021 taaaagaggg acttttatga gcggtggaga tggacgcggc cataacacgg gcgcgcatag
     4081 cacaagtggt aacattaatg gtggcccgac cgggattggt gtaagtggtg gtgcttctga
     4141 tggttcagga tggagttcgg aaaataaccc gtggggtggt ggttccggta gcggcattca
     4201 ctggggaggt ggctccggtc gtggtaatgg cgggggtaat ggcaattccg gtggtggctc
     4261 gggaacaggc ggtaatttgt cagcagtagc tgcgccagtg gcatttggtt ttccggctct
     4321 ttccactcca ggagctggcg gtctggctgt cagtatttct gcaagcgaat tatcggcagc
     4381 tattgctggt attattgcta aattaaaaaa agtaaatctt aaattcactc cttttggggt
     4441 tgtcttatct tcattaattc cgtcggaaat agcgaaagat gaccccaata tgatgtcaaa
     4501 gattgtgacg tcattacccg cagatgatat tactgaatca cctgtcagtt cattacctct
     4561 cgataaggca acagtaaacg taaatgttcg tgttgttgat gatgtaaaag acgaacgaca
     4621 gaatatttcg gttgtttcag gtgttccgat gagtgttccg gtggttgatg caaaacctac
     4681 cgaacgtcca ggtgttttta cggcatcaat tccaggtgca cctgttctga atatttcagt
     4741 taataacagt acgccagcag tacagacatt aagcccaggt gttacaaata atactgataa
     4801 ggatgttcgc ccggcaggat ttactcaggg tggtaatacc agggatgcag ttattcgatt
     4861 cccgaaggac agcggtcata atgccgtata tgtttcagtg agtgatgttc ttagtcctga
     4921 ccaggtaaaa caacgtcagg atgaagaaaa tcgccgtcag caggaatggg atgctacgca
     4981 tccggttgaa gcggctgagc gaaattatga acgcgcgcgt gcagagctga atcaggcaaa
     5041 tgaagatgtt gccagaaatc aggagcgaca ggctaaagct gttcaggttt ataattcgcg
     5101 taaaagcgaa cttgatgcag cgaataagac tcttgctgat gcaatagctg aaataaaaca
     5161 atttaatcga tttgcccatg acccaatggc tggcggtcac agaatgtggc aaatggccgg
     5221 gcttaaagct cagcgggcgc agacggatgt aaataataag caggctgcat ttgatgctgc
     5281 tgcaaaagag aagtcagatg ctgatgctgc attaagtgcc gcgcaggagc gccgcaaaca
     5341 gaaggaaaat aaagagaagg acgctaagga taaattagat aaggagagta aacggaataa
     5401 gccagggaag gcgacaggta aaggtaaacc agttggtgat aaatggctgg atgatgcagg
     5461 taaagattca ggagcgccaa ttccagatcg cattgctgat aagttgcgtg ataaagaatt
     5521 taaaagcttc gacgattttc ggaaggctgt atgggaagag gtgtcgaaag atcctgagct
     5581 tagtaaaaat ttaaacccaa gcaataagtc tagtgtttca aaaggttatt ctccgtttac
     5641 tccaaagaat caacaggtcg gagggagaaa agtctatgaa cttcatcatg acaagccaat
     5701 tagtcaaggt ggtgaggttt atgacatgga taatatccga gtgactacac ctaagcgaca
     5761 tatcgatatt caccgaggta agtaaaatgg aactgaagca tagcattagt gattatacag
     5821 aagctgaatt tttacagctt gtaacaacaa tttgtaatgc ggacacttcc agtgaagaag
     5881 aactggttaa attggttaca cactttgagg aaatgactga gcaccctagt ggtagtgatt
     5941 taatatatta cccaaaagaa ggtgatgatg actcaccttc aggtattgta aacacagtaa
     6001 aacaatggcg agccgctaac ggtaagtcag gatttaaaca gggctaaaat atgagtgccg
     6061 gttgtttaag gatgaatggc tggcattctt tcacaacaag gagtcgttat gaaaaaaata
     6121 acagggatta ttttattgct tcttgcagtc attattctgt ctgcatgggg ttctaagccg
     6181 aaaacctaga aaattccgta accaaagcca gtaattgaca gatttgcatg acgttgaata
     6241 ggtgacgggt tatgtgacga aatctgatgc agaaatcgtt gtttcagtga cagtcactca
     6301 atcggtcgtt tatgtgacaa cccacgccgt tactggtcgc ggaaaaatcc aggtttccgg
     6361 cctggaaccc cgaaatgatc cagcaacagt gtatggttct cctggtaaat atgttgttgt
     6421 caatgatcgt actggtgagg ttactcagat tagtgataag acagatccgg gttgggtgga
     6481 cgattcgaga attcaatggg gaaataaaaa tgaccaataa attatttgaa catacggtgt
     6541 tatatgatag tggtgatgcc ttttttgaat taaaaggaaa tgcttctatg aagttatcac
     6601 caaaagctgc aatagaagtt tgtaatgaag cagcgaaaaa aggcttatgg attttgggca
     6661 ttgatggtgg gcattggctg aatcctggat tcaggataga tagttcagca tcatggacat
     6721 atgatatgcc ggaggaatac aaatcaaaaa tccctgaaaa taatagattg gctattgaaa
     6781 atattaaaga tgatattgag aatggataca ctgctttcat tatcacgtta aagatgtaaa
     6841 tagtgttata gaattttatg tttcatggat gatttcaacc tttggatttc aggtttttat
     6901 ggatgaatgc ctgagtccat atatacaagg aacagtatga aaaaaataac agggattatt
     6961 ttattgcttc ttgcagccat tattcttgct gcatgtcagg caaactatat ccgcgatgtt
     7021 cagggcggga ctgtatcccc gtcatcctca gctgaactga ccggattagc aacgcagtaa
     7081 cccgaaatcc tctttgacaa aaacaaagcg tgtcaggctg attctgatgc gctttttttt
     7141 tgaaatgtca caaaaattcc atgtgggaga tgggatctaa aatccccgtg cagaactttc
     7201 catcctgggg gagaaaactt gtcgttttga gccgttcggt gttcagaacg cacgaaaccg
     7261 atcgcgcatc gctttcgtgc atagttatgc aacgcgctaa aaacgattct gacgcatttt
     7321 ttatgtttac cctggtgttt ccctgtcttt ttgcgttttt tgcgtcagaa cgcgtctgag
     7381 agcgttttaa ggggtgcgta caacaggagt tatggtaaat cggaggtgtt tatggcttac
     7441 gacgaacatt cgattcgcgt gatgtctgct gacgagatcg agcaacgttt tgactggttg
     7501 cgcctggaga acctggcgaa ggagcatcgc ctacccgttg actgggtccg acggggattt
     7561 gaagcctgct ggcgactggg tatcgagccg gattatttca tcgacaggta tatcttcaaa
     7621 cgggatgtgc cactggtgcc ggagtttgag gtcgtgttca gggagatcgt gaacgaaaac
     7681 cgatacaggg atcggatgcg tttctgatat gcctctaaaa acgcctgtaa gcgattctga
     7741 cgcgtttttc tgatttgggt gaggtttttt acgtccatat ccactttttt gcgtcacaga
     7801 gcgattgaga gcgttttaga gggtacgtac aacgggagtt atggtaaatg gatcgggttt
     7861 tggggaataa tcgacagggt ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc
     7921 cccgtaaatc gtgctctcac cgccaagatt gatcacgaaa ttacagggcg ccgggttccg
     7981 cgtctcccga tgggaaagcg cggttagtta aactgtgtac cgagaggaat cgtaacac
//
