LOCUS       NZ_CP100535             8910 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Escherichia coli strain LH10-b-1 plasmid pLH10-b-1-E, complete
            sequence.
ACCESSION   NZ_CP100535
VERSION     NZ_CP100535.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499312
            Assembly: GCF_024225755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,291
            CDSs (total)                      :: 5,175
            Genes (coding)                    :: 4,870
            CDSs (with protein)               :: 4,870
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 99 of 305
            Pseudo Genes (incomplete)         :: 210 of 305
            Pseudo Genes (internal stop)      :: 61 of 305
            Pseudo Genes (multiple problems)  :: 59 of 305
            Pseudo Genes (short protein)      :: 4 of 305
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100535.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LH10-b-1"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pLH10-b-1-E"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            complement(join(8809..8910,1..285))
                     /locus_tag="NLZ08_RS26195"
                     /old_locus_tag="NLZ08_26200"
     CDS             complement(join(8809..8910,1..285))
                     /locus_tag="NLZ08_RS26195"
                     /old_locus_tag="NLZ08_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_085448100.1"
                     /translation="MTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSELIRAYGELKQD
                     ETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERRRQEAEREQLHK
                     EIEQLRQALEQEKKRGFWARLFSR"
                     /name="entry exclusion protein 1"
     gene            596..2161
                     /locus_tag="NLZ08_RS26200"
                     /old_locus_tag="NLZ08_26205"
     CDS             596..2161
                     /locus_tag="NLZ08_RS26200"
                     /old_locus_tag="NLZ08_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_209200378.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETKRQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(2158..2499)
                     /locus_tag="NLZ08_RS26205"
                     /old_locus_tag="NLZ08_26210"
     CDS             complement(2158..2499)
                     /locus_tag="NLZ08_RS26205"
                     /old_locus_tag="NLZ08_26210"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058758.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            2547..2684
                     /locus_tag="NLZ08_RS26210"
                     /old_locus_tag="NLZ08_26215"
     CDS             2547..2684
                     /locus_tag="NLZ08_RS26210"
                     /old_locus_tag="NLZ08_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001519492.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3709..3900)
                     /locus_tag="NLZ08_RS26215"
                     /old_locus_tag="NLZ08_26220"
     CDS             complement(3709..3900)
                     /locus_tag="NLZ08_RS26215"
                     /old_locus_tag="NLZ08_26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3965..4288
                     /locus_tag="NLZ08_RS26220"
                     /old_locus_tag="NLZ08_26225"
     CDS             3965..4288
                     /locus_tag="NLZ08_RS26220"
                     /old_locus_tag="NLZ08_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4497..>4928
                     /locus_tag="NLZ08_RS26225"
                     /old_locus_tag="NLZ08_26230"
                     /pseudo=""
     CDS             4497..>4928
                     /locus_tag="NLZ08_RS26225"
                     /old_locus_tag="NLZ08_26230"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4966..5484
                     /locus_tag="NLZ08_RS26230"
                     /old_locus_tag="NLZ08_26235"
     CDS             4966..5484
                     /locus_tag="NLZ08_RS26230"
                     /old_locus_tag="NLZ08_26235"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            5491..5724
                     /locus_tag="NLZ08_RS26235"
                     /old_locus_tag="NLZ08_26240"
     CDS             5491..5724
                     /locus_tag="NLZ08_RS26235"
                     /old_locus_tag="NLZ08_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(<7604..7780)
                     /locus_tag="NLZ08_RS26240"
                     /old_locus_tag="NLZ08_26245"
                     /pseudo=""
     CDS             complement(<7604..7780)
                     /locus_tag="NLZ08_RS26240"
                     /old_locus_tag="NLZ08_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(8306..8725)
                     /locus_tag="NLZ08_RS26245"
                     /old_locus_tag="NLZ08_26250"
     CDS             complement(8306..8725)
                     /locus_tag="NLZ08_RS26245"
                     /old_locus_tag="NLZ08_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          3537..3624
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(2968..3514)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4279..5828
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctggcgccgg cgttccgtag cctgtgcctg tttgtcctca agcatcagtg tcaggcattg
       61 tctcagctca ttcagctccc ggagaatgcg ttctgtctgc tgatcatgtg gattttctgc
      121 ctgtcccgca ctgtgccttt ctggtgtctc atcctgcttt aattcgccgt atgcccggat
      181 cagttcactg gtttcaaact cccgccgtcc gtcctggcct gtacgataac tgacaaggcc
      241 tgcgcgcata tcacggtaaa actgtgaacg cgatctgcct gtcattttca gtgcgttctg
      301 gagtgtgtgc catgccatga agtgacagtg tcccattaat gtctcatatc atggtttcag
      361 taaaacatac tggggccgga gaacggtaat gtttgtgctg gtttttgtgg catcgggtgg
      421 gaatagcacg tggtgtgggt ggctgttttt ttgatcgttt tcacaaaaat gaaaatccac
      481 agggttgaca gggaaaatgc agcggcgtaa ttttaagctg tatataaaac cagtggttat
      541 acatacagta tattgctttt aactatttgt ttttaatgtc aaagaggatt ttataatgga
      601 aacagctgta gcgtactata aagatggtgt tccttatgat gataaggggc aggtaatcat
      661 tactcttttg aatggtaatc cagacgggag tggctctggc ggcggtggtg gaactggagg
      721 tagcaaaagt gaaagttctg cagccattca tgccacagct aaatggtcta ctgctcaatt
      781 gaagaaaacg caggcagaac aggctgcccg ggcaaaagct gccgcagaag cacaggctaa
      841 agcaaaagca aaccgggatg cgctgactca acatctgaag gatattgtga atgaggcgct
      901 tcgtcataat tccactcatc cggaggttat tgacctggct catgccaata atgcagcgat
      961 gcaggcagaa gcagagcggt tgcgccttgc aaaagcagaa gaaaaagccc gtaaagaagc
     1021 ggaagctgcg gaaaaggctt ttcaggaggc agaacaacga cgtaaagaga tagagaagga
     1081 gcaggctgaa acaaaacgcc agttgaaact ggctgaagct gaagagaaac ggctggcagc
     1141 attgaatgaa gaggcccggg cagtggaggt ggcacaaaaa aatcttgctg ctgcacaatc
     1201 tgagctggcg aaagtggatg aagagattaa gactctcaat acccgtttaa gctccagtat
     1261 tcatgcccgt gatgcagaaa tgaatacgct gtccggaaaa cgaaatgagc tggctcaggc
     1321 atctgctaaa tataaggaac ttgatgaact ggtcaaaaaa ttgtcaccaa gagctaatga
     1381 tccgcttcag agtcgtcctt ttttctatgc caccagtcga cggacgggag ccggtaagat
     1441 tatggaggaa aaacaaaaac aggtaacagc atcagaaacg cgtattaacc aacttaatgc
     1501 tgagataaat ggaattcagg gggctatgtc tcaggctaat aataatcgta atacagctgt
     1561 tttacgtgtt catgaatctg aagaaaattt gaaaacagcg cagactaatc tcctgaactc
     1621 gcagattaag gatgctgtgg atgcaacagt tagcttttat caaacgctat ctgaaaaata
     1681 tggtgaaaaa tattcaaaaa tggcacagga acttgctgat aagtctaaag gtaagaaaat
     1741 cagcaatgtg aatgaagctc tagctgcttt tgaaaaatac aaggatgttt taaataagaa
     1801 attcagcaaa gcagaccgtg atgcgatttt caatgcactg gaatcggtta agtatgaaga
     1861 ctgggctaag catttagatc agtttgctaa gtacttgaag attacgggac atgtttcttt
     1921 tggatatgat gtggtatctg atatcctaaa aattaaggat acaggtgact ggaagccact
     1981 atttcttaca ttagagaaga aaactgtaga tgctggagtt agttatattg ttgttttact
     2041 ttttagtgtg cttgctggaa ctacattagg tatctggggg attgctattg ttacaggcat
     2101 tctatgtgcc tttattgaca agaataaact taatactata aatgatgtgt tgggtattta
     2161 attattcttt attgatataa acagtcctag caatccaaat gggattgcta ggaccaaaca
     2221 aagtagattg tatagcataa ataggtttaa ttttgccacg ggggcattat ttagattttt
     2281 tctttttctg aaaaaatctt tctttatgaa cttaaaaaat atgtgttcaa tagcatattt
     2341 tgaatatggg catagaatag tgcttatcac gattgcgtat agcatcttat ctgacgctag
     2401 gaaataatac ccttcgttgt tttttgttat aaggtatata tatataagtg tgcaatatag
     2461 gccaaacaaa atgtttttta tgtagtatct taaactcata aattaaacct cgttatatat
     2521 tcttttcatt ttataaggat cgagttatga ggaaaagatt ttttgtggga atattagcta
     2581 taaacctcct tgttggatgt caggctaact atatacgtga tgttcaggga gggactgtgg
     2641 ctccatcatc ttcttctaaa ctgacgggga tctctgttca gtagaaaaga tcaaaggatc
     2701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcctg taaacgaaaa aaccgcctgg
     2761 ggaggcggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgag gtaactggct
     2821 tcagcagagc gcagatacca aatactgtcc ttccagtgta gccgtagtcg ggccactact
     2881 tcaagaactc tgttgcacca tttgcgccat catcgctctg ctaaccaagt taccaacggc
     2941 tgctgccagt ggcgctttgt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3061 gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga
     3121 agggagaaag gcggagaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3181 ggagcttccg gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga
     3241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaagcgcctc
     3301 cggcgcggcc ttttctgggg tcttcgtttt ttgctcacat gttctttccg gcgttatccc
     3361 ctgattgtgt ggataacagt atcaccgctt ttgagtgagc agacaccgct cgccacagcc
     3421 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcatcgg catctgcatg
     3481 ttctccttac gcatctgtgc ggcatttcac accgtacatg gtgcactttt catacaatcc
     3541 gcaccgatgc cgcatgacta agccagtata cactccgcta tcgctacgtg actgggtcag
     3601 ggctgcgccc cgacacccgc taaaacctgc tgacgcgccc tgacgggctt gtcggctccc
     3661 ggcatccgct cacagacaag ctgtgagcgt ctccgggagc tgcatgtgtc agaggttttc
     3721 accgtcatcc ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag
     3781 cgattcacag atatctgcct gttcatccgc gtccagttcg ttgagtttct ccagcagcgt
     3841 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttagtcac
     3901 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgcga
     3961 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     4021 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     4081 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     4141 cctgaatcag accgcgagga aggtgaacag cgggcagtgg tcttccggtg atcgggttca
     4201 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggccgtcag
     4261 ggaacagggg gcgcgggatg atagttaaat ttcatgccag gggaaaaggt ggtggcagtg
     4321 gtccggttga ttacctgctg gggagggagc gtaaccgcga aggcgcaacg gtgcttcagg
     4381 gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     4441 caggcgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa aaagtgatgg
     4501 cgagctttga gcgcgtactg atgccaggtc tcgaaaagaa tcagtacagc atcctgtggg
     4561 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     4621 agaccggaaa acgtctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     4681 ggcagacgct ggtgaatcac cattacgggc tgcatgaccc caacgccccg gagaaccgcc
     4741 ggatactgac tctctctgat aacctgccgg aaacgaaaca ggcgcttgct gagagcgtca
     4801 cacgcgggat agacgctctt tatcatgtag gggagataaa aggccgtcag gatgtgattc
     4861 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagc atcagcattg
     4921 cagacccgaa cggcgggaag aatatcaggc tgaaaggagc gttttatgag caatctttta
     4981 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaagtg
     5041 ctgaacaacg agttcaggag gctcggcgaa tctgtaagcg aggctgtgac atcaaacgag
     5101 acgaaaatca gagacgctat agccctgttc acagcctcga cagaggaatc gctggagaaa
     5161 caccgggaag gggtgaaaga ggcgatgagg cagcacagga aggacgtgtt aaagctggca
     5221 gggaatacgg gcatgatgtt actggggata gtctttctcc tgtttaccgc gagtggcggg
     5281 acgctctggt atcttggcgg gaggatacag gcgaacctgg aagatatcag gaagcaggaa
     5341 gagacattgc agaaactgaa cgcgaagaca tggggcgtgg agttcgtgca ggacgggaac
     5401 aggaaatttc tggtccttcc gtacgggaaa tcagcagagg tgattccctt tcaggggaaa
     5461 gagtgggtac atctgaagga gtgacacaga gtgacagagc tggaaacaca tttgctgaac
     5521 gccttagagc agctgcaaca ggactatatg cagcggctga acgaatggga gagcgccttc
     5581 gtggaattgc agaagatgtt ttcgcttacg caacgggaca acgcgatgct gaacgagcgg
     5641 gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc agacagaaca cttgagccag
     5701 ttatacagcg agagcaggag ataagggacg aactactgat acaggaacgt gaacatgagc
     5761 tattcctaga gcgtgagcgc catctggaaa tacaggaacg gacactggat ggcccgtcgc
     5821 tgggatggta accaaagaca ggctaaagtg ttgtcagcgc gtttccggtg catttttttg
     5881 gataatggtt ggttcagggc actaggaact agcttcagtc aggccttgct gaaaacacag
     5941 gaacgcgtta aaacgcatac ggacgcgttt ctggcgctca aaaaccatgg ggttagtgtg
     6001 ggtagtgtgt atagcaacag gagcctggaa cgcgttccgc ttcttctgta atcgacgcca
     6061 gaatgcgttt aatgcttctt ctgatggtat cagagaggag ctgcttgagt tattcctgtt
     6121 ggccacagag cgtaattatc tccttttcag atccacacgg ggatacgtca gaatcggaac
     6181 tgagcaagga ttactgaaag atactgtttg ccagaaaaaa aatcctcata catgatgagg
     6241 ataaagtctt aaaggtccat tctatggagt gtcggattga aaaatttctc cggatgatat
     6301 ccttcacagt ttaaatgtga tttcaaccat aagtttttta tggattttgt taaataaaaa
     6361 agaattgagt cgtgtttctg atatgtctga atataataag tgaacagtta taaaataatt
     6421 gttttatatt tattgtgagt ttgatgttat gttctgcata aataactgta gtttagaata
     6481 ttattctttt atatttatag ctgtgagatg ataaattata agtgttcttt gtgctataaa
     6541 gaaccccccg tataaggggg ggtaaaagat gggcttactt ctttttagtc catgccctgg
     6601 tgagctacat gaagctcgac actacagtaa aaatttgcag tttttttgaa ctttacgcaa
     6661 gcaatttatt tacattgtaa agaaaacccc ctcaattccg gagggggagt gtagggaagt
     6721 gcctgattta ttttatgtac gcgcaaaaat gagatagctt ttattagata tcatcagccc
     6781 tgtctgcatc agtcagctcc ttacagcgtc aggagtactg atgaaggggg gaacgttcct
     6841 gcgtttttgc aaaggcaatc cggctagagg ctttactacg cagaaacatt ccggacaact
     6901 ttttacccca gtgtaacttg tagatcaacc agattttgtt gcagtaaaaa tacagtggtt
     6961 tcgaatttgt aacatttgtc acaaaaataa catcaatatg ttggttttct gttgataaat
     7021 ggtttggtcg ctgattatga tttccttgta ctccacagga gtacagtcat gtgcatacta
     7081 aaatccgtgt tttcgataaa attaaggccg tgatacagaa tcacggcttt tttcttgcct
     7141 gaaaggatca tttacgctag aagaattatg agcgcgtctc tggtgtttgt tgcagggata
     7201 atatttaata tggggcaccg gaaacgcgct ctcccttcgt atacagaatg cccattaatt
     7261 tcttcttcct gattttccag acatatattc ttgtgtaaat taaaaaacag agcgtgtttc
     7321 tggtgtgaat ttgttggcta caatgtgcat tgttgttctg tgttccagaa acacgcttct
     7381 tttttgagat gcacatcggt cttatacaaa gagggatggt tgtatctgtg tcagagtttt
     7441 gttatttttt gtatgctcag ttattgatgg ttaataatat caagaaaatc agcaagttcc
     7501 ttacactacc cctcttcagt agagggtttt ttttatctgg tacgccgtaa aacccggtcc
     7561 ttcagggcgt agaggatgtc aaaaatttct tcgaaaggta atacccggta tcccgctcat
     7621 gctggcgaac ccagacacgc agggtctctg gcgtacagcc aatctttgga gcaatggaac
     7681 aaattgccgc ccattgtgag tcatattcgc cctggctttc cagaaccata cgaactgccc
     7741 gttgacggac ttcgggggaa aaacgagtat ttttagtcat cctgtttaca tctttctcag
     7801 gaagtttagt ctccaggatt tccggggcgg ttcagtccgg cagagataaa ttgccacggg
     7861 aagttagaga aaagtgcggg aaaatgtctt aagaatgatt ctggaagaac gaaaagtaag
     7921 aagcgcaaag gctatgaggg caaaagagta tcgtgccgca gcgaatactt ttacctggca
     7981 gcctgtacag cgcaggtgaa tagacattcc cctgactcat cctgctcccc ttggcggcga
     8041 aggggattaa tccctatcct gtaacttcta ttttgtggaa tcgaaaacag ctgaaaattg
     8101 tgaaaaaaaa ccgtttcagt cgtctcagta aggaaattcg cttgtgattc acctggatgc
     8161 ataggtatgc ataaggttaa aatttatcag gcgcgatcgc ggtagttttt cgggggggtt
     8221 gttgcgattt ttacctgtct gctgccgtga ttgcgctgaa tgcatttcag cggtgcgtac
     8281 aattaaggga ttatggtaaa tccacttact gtcttccctc gtagccatcg agataaaccg
     8341 cagaaatcgt ctcagccagc agcctggatt ttttcggggt aaggtctttg ctgcggtaat
     8401 cagtacccgc aaattgagtt gtgccccgga gtgagtttaa aaattcctgg ctgtgaaact
     8461 gctgaatgcg ttgctgcaca tcactccgtg attttcctgt cgctttatcc tttgctccca
     8521 tatcccagaa ctgccggaaa aaaggcacca tcattcgtgt tgtgtcggcc ttctgggtgg
     8581 aaaagttagg atcaaaacca tcgtctgatg cataaacgta atgccgggta tgttgttcca
     8641 cactgccctt tggcgtgcag gctgtcagga ccgttgccgc tgtcagcagg gcgataagat
     8701 acagtgtgcc gccggaacgt ctcatgagtg tctccttacc tttaaatctt tgtactcagt
     8761 gacttatatt taaccgctga ctatcttatc atcacttgca gtcggtgctc accggctgaa
     8821 cagtctggcc cagaatcccc gttttttttc ctgctccagt gcctgcctga gctgctctat
     8881 ctctttatgt agctgttccc gttcggcttc
//
