LOCUS       NZ_CP100534             9400 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Escherichia coli strain LH10-b-1 plasmid pLH10-b-1-D, complete
            sequence.
ACCESSION   NZ_CP100534
VERSION     NZ_CP100534.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499312
            Assembly: GCF_024225755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:38:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,291
            CDSs (total)                      :: 5,175
            Genes (coding)                    :: 4,870
            CDSs (with protein)               :: 4,870
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 99 of 305
            Pseudo Genes (incomplete)         :: 210 of 305
            Pseudo Genes (internal stop)      :: 61 of 305
            Pseudo Genes (multiple problems)  :: 59 of 305
            Pseudo Genes (short protein)      :: 4 of 305
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100534.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LH10-b-1"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pLH10-b-1-D"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            complement(join(9299..9400,1..318))
                     /locus_tag="NLZ08_RS26135"
                     /old_locus_tag="NLZ08_26140"
     CDS             complement(join(9299..9400,1..318))
                     /locus_tag="NLZ08_RS26135"
                     /old_locus_tag="NLZ08_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_136710330.1"
                     /translation="MAWHTLQDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRREFETSE
                     LIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQAQDTERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="entry exclusion protein 1"
     gene            562..2451
                     /gene="cra"
                     /locus_tag="NLZ08_RS26140"
                     /old_locus_tag="NLZ08_26145"
     CDS             562..2451
                     /gene="cra"
                     /locus_tag="NLZ08_RS26140"
                     /old_locus_tag="NLZ08_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin R"
                     /protein_id="WP_254547659.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGVTSTQARQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLTPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKEMGRVTERIVDAINLALILNQTE
                     SRLSESQKNVDVANQIISDTVKAINDVNKKIAEKRNQQVSLTDLMNKKQKEVEDLKKIF
                     KNHSYHRIRDAQREYDDARNKYALLASDINALQAQVSGLTARKQQAEQNKAAAEKAKAD
                     AAAKAAAEKAAAEAKAKAEAEKARKEAEEKANDEKAVLTKASEIIISVGDKAGEYLGDK
                     YKVLSREIADNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDAIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYMMKANNVREKSIEGYQTGNWGPLMLEIESWVLSGIASAVAL
                     SFFSVAVGSLFITAGVSVTAVGLMGIIFAGFIGALIDDYFVDKLNNEIIRPAY"
                     /name="cra"
     gene            complement(2479..3003)
                     /gene="cri"
                     /locus_tag="NLZ08_RS26145"
                     /old_locus_tag="NLZ08_26150"
     CDS             complement(2479..3003)
                     /gene="cri"
                     /locus_tag="NLZ08_RS26145"
                     /old_locus_tag="NLZ08_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089619593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R immunity protein"
                     /protein_id="WP_089619593.1"
                     /translation="MQKEYTYNKDLNRMLFLITFAGIIPLIIIFITYTSNPNFYLIRII
                     FDNTQNIPSVISSYNPVMTKVMDIYGKSAPLLAFIAFIMLFKHTRTTKITDRKKIITAC
                     ILSPFLCIFYAYFFLWHNFELTTSGRPVRLMSENDLTLLIFYICLYYCSFFITYALCLQ
                     PVIAYKLWKER"
                     /name="cri"
     gene            3123..3263
                     /gene="crl"
                     /locus_tag="NLZ08_RS26150"
                     /old_locus_tag="NLZ08_26155"
     CDS             3123..3263
                     /gene="crl"
                     /locus_tag="NLZ08_RS26150"
                     /old_locus_tag="NLZ08_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_072020761.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSELTGIAV
                     Q"
                     /name="crl"
     gene            complement(3481..3594)
                     /locus_tag="NLZ08_RS26155"
                     /old_locus_tag="NLZ08_26160"
     CDS             complement(3481..3594)
                     /locus_tag="NLZ08_RS26155"
                     /old_locus_tag="NLZ08_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071699851.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(4264..4455)
                     /locus_tag="NLZ08_RS26160"
                     /old_locus_tag="NLZ08_26165"
     CDS             complement(4264..4455)
                     /locus_tag="NLZ08_RS26160"
                     /old_locus_tag="NLZ08_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4520..4843
                     /locus_tag="NLZ08_RS26165"
                     /old_locus_tag="NLZ08_26170"
     CDS             4520..4843
                     /locus_tag="NLZ08_RS26165"
                     /old_locus_tag="NLZ08_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_052928905.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGEELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5052..>5483
                     /locus_tag="NLZ08_RS26170"
                     /old_locus_tag="NLZ08_26175"
                     /pseudo=""
     CDS             5052..>5483
                     /locus_tag="NLZ08_RS26170"
                     /old_locus_tag="NLZ08_26175"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5521..6051
                     /locus_tag="NLZ08_RS26175"
                     /old_locus_tag="NLZ08_26180"
     CDS             5521..6051
                     /locus_tag="NLZ08_RS26175"
                     /old_locus_tag="NLZ08_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_077817679.1"
                     /translation="MSNLLQTGAEFEKKLKERAASTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINAHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETHWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            6058..6291
                     /locus_tag="NLZ08_RS26180"
                     /old_locus_tag="NLZ08_26185"
     CDS             6058..6291
                     /locus_tag="NLZ08_RS26180"
                     /old_locus_tag="NLZ08_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_071699844.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            6322..6618
                     /locus_tag="NLZ08_RS26185"
                     /old_locus_tag="NLZ08_26190"
     CDS             6322..6618
                     /locus_tag="NLZ08_RS26185"
                     /old_locus_tag="NLZ08_26190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136710329.1"
                     /translation="MSYPWSVSAIWKYRNGHWMARRWDGNQRQAKMLSARFRCIFLDNG
                     WFGAPGTSFSQALLKTQERVKTHTDAFLAVKNHGVSVGSVYSNRSLERVPLLL"
                     /name="hypothetical protein"
     gene            complement(8796..9215)
                     /locus_tag="NLZ08_RS26190"
                     /old_locus_tag="NLZ08_26195"
     CDS             complement(8796..9215)
                     /locus_tag="NLZ08_RS26190"
                     /old_locus_tag="NLZ08_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          4092..4178
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.47e-38"
     Typing          complement(3523..4066)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.055"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4834..6395
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.393"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttggcgccgg cgttccgtat cctgtgcctg tttgtcctca agcatcagtg tcaggcattg
       61 tttcagctca ttcagctccc ggagaatgcg ttctgtctgc tgatcatgtg gattttctgc
      121 ctgtcccaca gtgtgccttt cttgtgtctc attttgcttt aattcgccgt atgcccggat
      181 cagttcactg gtttcaaact cccttcgtcc gtcctggctg gtacggtaac tgacaagacc
      241 tgagcgcata tcacggtaaa actgtgaacg cgatcttcca gtcattttca gtgcgtcctg
      301 gagtgtgtgc catgccataa agttacagtg tcccataggt gtctcatctc atggtttcag
      361 taaaacataa tgaggtctga gaacggtaat gtttgcgccg gtttttgtgg tatccggcgg
      421 gaatagcgcg tggtgtgaga gactgttttt ttgatcgttt tcacaaaaat ggaagtccac
      481 agtcttgaca gggaaaatgc agcggcgtag cttttatact gtacataaaa ccagtggtta
      541 tgtgtacagt atttgttttt aatggtttgt tttaaaagtc aaagaggaat gaatatgccg
      601 gggtttaatt acggtgggta tggtgacggt actggctgga gttctgaaag tggtggtcca
      661 gctccaggcg gtggtatgca tggaaatagt ggaggccagc gcggagataa cgcaaacagt
      721 agcaattctg tgagtcagca gatttctgcc attcagaatg atcagaaact taaacagaaa
      781 gtggtgaata tgctgattgc tgcgagaaaa atgaatcctg aagcaaaaat gattctggga
      841 agtattgctc cttctggtgt tatgcaggtc accattgagg gggtaacctc cactcaggcc
      901 aggcagcttg gtttgggagg gctggtcatg ggatataacg cttctggtgt tattggggct
      961 gtgggcgaaa tagacaccgg tcaccgcctt aatgcatcag gagcaagtac tcccggaagc
     1021 gaaacttctg tggagagttt tgtgaacgga cagaaaccag ccggagaatg gcatgctgtg
     1081 gcgaaggaca gctggacagg ggctggtcca gtgaatgtag ggctggttaa taacgccatc
     1141 aaaagtgtcc ggattatcaa aaaaggctat gtgacgggcg ttctcacgcc agaagaggtg
     1201 atgaataaag cagagtacaa agcaatgcgg caggctttcg attcccttcc actggcaaaa
     1261 cagggagagg ctgtcagaca gattgttgct gcttggagtc tggcatatca ggattttccg
     1321 gtaaatctga aaaaagagat ggggcgtgtc actgaacgga ttgttgatgc tattaacctg
     1381 gcgctcattc ttaatcagac agagtcccgt ttgtcggaat cacaaaagaa tgttgatgta
     1441 gcgaaccaga ttatcagtga tacagtaaaa gctattaatg atgtaaataa gaaaatagca
     1501 gaaaaacgaa atcagcaggt atcactgact gatttgatga ataaaaaaca aaaagaggtc
     1561 gaagatctta aaaagatatt caaaaatcac tcttatcacc gtattcgtga tgcccagagg
     1621 gaatatgatg atgcccgaaa taaatatgct ttactggcca gtgatattaa tgcgctgcag
     1681 gcacaggttt ccggactgac ggcaaggaaa cagcaggcag aacagaacaa ggcagcagca
     1741 gaaaaagcaa aggcagatgc agcggcgaaa gctgcagcag aaaaagcggc tgctgaagcg
     1801 aaagcaaagg ctgaagctga aaaagccaga aaggaagcag aggaaaaagc aaatgacgag
     1861 aaggctgttc tgacaaaagc cagtgaaatt atcatcagtg ttggtgataa ggctggggag
     1921 tatctgggtg ataaatataa agttctttct cgtgagatag cagataatat caaaaacttt
     1981 cagggcaaaa cgattcgtag ttatgatgag gcgatggctt ctgtcaataa actgatggct
     2041 aatcctgatc ttaaaattaa tgccgccgac agggatgcaa ttgtgaatgc ttggaaagca
     2101 tttgatgcag aggatatggg gaataagttt gccgcgctgg gtaagacgtt taaagccgca
     2161 gattatatga tgaaggcaaa caacgtccgg gagaagagta tcgaggggta tcagaccgga
     2221 aactgggggc cgctgatgct ggagattgag tcctgggtat tgagtggtat tgcttctgct
     2281 gtggcgctat catttttctc agtcgctgtt ggttcattat ttattacagc gggcgtgtct
     2341 gtaacggctg tgggacttat gggcattatt tttgctggct ttatcggtgc gctgattgat
     2401 gattattttg ttgataagtt aaataacgaa attattcgcc ctgcgtatta attgagagtc
     2461 gaaaggtagt caggaggact acctttcttt ccagagttta tatgctatta ctggttggag
     2521 gcagagggcg taagttatga aaaaagagca ataatacagg cagatataaa agataagcaa
     2581 agtaagatcg ttctcagaca tcaatcgaac aggtcttccc gatgttgtta actcgaagtt
     2641 atgccacaag aaaaaataag cataaaaaat acacaaaaag ggggacagta tacaagctgt
     2701 tatgattttt tttctgtctg ttattttggt ggttcttgtg tgtttgaata gcatgatgaa
     2761 agctataaat gctaataatg gtgcgctctt accgtagata tccatcacct ttgtcatcac
     2821 agggttatat gaagaaatta ctgaaggtat attttgagta ttatcgaaaa tgatgcggat
     2881 taaatagaag ttggggtttg atgtataagt tataaaaata ataattaatg ggattatacc
     2941 cgcgaaagta atcaaaaaaa gcatcctgtt taaatctttg ttgtaagtgt actctttttg
     3001 cattggtata atactcccgg tttttcattg ttattaattt agttgttgat atgtataagc
     3061 atatcatgct gtgcgagtaa tggtcaccct aatattaggg gtatgagtta atgaggggat
     3121 atatgaaaat gatattatca ggagggatgc ttgtcttgat ggctttgcta tcaggctgcc
     3181 aggctaatta tattcgcgat gttcagggag ggaccgtcgc accatcctcc tcttctgaac
     3241 tgacgggaat cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct
     3301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccaacggtgg tttgtttgcc
     3361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttggaggagc acagtcacca
     3421 aaacctgtct tttcagttta gccttaaccg gctcataact tcaagaccaa ctcctctaaa
     3481 tcagttacca gtggctgctg ccagtggcgc tttgtcgtgt cttaccgggt tggactcaag
     3541 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3601 cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag
     3661 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaaacggca gggtcggaac
     3721 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3781 gtttcgccac ctctgacttg agcgtcaatt tttgtgatgc tcgtcagggg ggcggagcct
     3841 atggaaaaac gccagcggcg cggcctttcg ttgccctttg cctttttgct cacatgttct
     3901 ttccggcctt atcccctgat tctgtggata accgtattac tgcctttgag tgagctggca
     3961 ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc
     4021 gtcggaagtt gcattttctc cttacgcatc tgtgcggcat ttcacatcgg ctatggtgca
     4081 cttttcatac aatccgcact gatgccgcat gactaagcca gtatacactc cgctatcgct
     4141 acgtgactgg gtcatggctg cgccccgaca cccgctaaca cccgctgaag cgccctgatg
     4201 ggcttgtctg ctcccggcat ctgctcactg acaaactgtg accgtctccg ggagctgcat
     4261 gtgtcagagg ttttcaccgt catccccgaa acgtgcgagg caactgcggt aaagctcatc
     4321 ggcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gttcgttgag
     4381 cttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcagtttt
     4441 ttcctgttta gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     4501 accgatgaaa cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     4561 actggaacgc tgtgagggta aacaactggc ggtatggatg cggcgggtct gcctgggtga
     4621 gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc
     4681 cgccatcggg aataacctga atcagacagc gaggaaggtg aacagcgggc agtggtcttc
     4741 cggtgaccgg gttcaggtag tggccgcact gatggccatc ggggaggagc tgcgccggct
     4801 gcgtctggcc gtcagggaac agggggcgcg ggatgatagt taagtttcat gccaggggaa
     4861 aaggtggtgg cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg
     4921 caacggtgct tcagggtaat ccggaagaag tccgggaact catcgatgcc acgccatttg
     4981 cgaagaaata cacgtcaggt gttctgtcgt tcgcggagaa ggagctgccg ccgggaggac
     5041 gtgaaaaagt gatggcgagc tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt
     5101 acagcatcct gtgggtggag caccaggaca agggacggct tgagctgaat tttgtcattc
     5161 cgaacatgga gctacagagc ggaaaacgcc tccagccgta ctacgaccgg gcagacaggc
     5221 cgagaattga tgcctggcag acgctggtta atcaccatta cgggctgcat gaccccaacg
     5281 ccccggagaa ccgccggacg ctgactctcc ctgataacct gccggaaacg aaacaggcgc
     5341 ttgctgagag cgtcacacgc gggatagacg ctctttatca tgtcggggag ataaaaggcc
     5401 gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcaga gtgacgcgaa
     5461 gcagcatcag tattgcagac ccgaacggcg ggaagaatat caggctgaaa ggagcattct
     5521 atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgca
     5581 tctacagaga aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa
     5641 ctgacctcaa acgaatcttt aatcaggaac gctatcaacg cccatacagc agccctgaaa
     5701 gaacttctgg agaggtatca gaaaacgacc gtcagcacga tggacacaca ctggcaaacc
     5761 gtgctgaaaa tgagcgtgaa acgctggctg tggctgatta tcgtctcagt gctgatgttt
     5821 gccacgaccg ggagtctgct gtggtaccag ggcatgaaga taaacgcaaa catgaatatt
     5881 ctccgcgagc agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat
     5941 cacgaggaca gcaacggacg ttttctggtg ttgccgaagg ggatgaaagc cgaaacgcac
     6001 tggacggagg acaacggcaa actgaacgca gtgagactgg tacaggagtg acagagcatg
     6061 acggagctgg aaaaacattt gctgaacgcc ttagagcaac tgcaacagga ctatatgcag
     6121 cggctgaacg aatgggagag cgccttcgtg gaattgcaga agatgttttc gcttacgcaa
     6181 cgggacaacg cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg
     6241 agcgagcgga cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac
     6301 ggctgataca ggaacgcgaa catgagttat ccctggagcg tgagcgccat ctggaaatac
     6361 aggaacggac actggatggc ccgtcgctgg gatggtaacc aaagacaggc taaaatgttg
     6421 tcagcgcgtt tccggtgcat ttttttggat aatggttggt tcggggcacc aggaacgagc
     6481 ttcagtcagg ccttgctgaa aacacaggaa cgcgttaaaa cgcatacgga cgcgtttctg
     6541 gcggtcaaaa accacggggt tagtgtgggt agtgtgtata gcaacaggag cctggaacgc
     6601 gttccgcttc ttctgtaata gacgccagaa tgcgtttaat gcttcttctg atggtatcag
     6661 agaggagcag cttgagttat tcctgttggc cacagagcgt aattatctcc ttttcagatc
     6721 cacacgggga tacgtcagag taggaactga gcaaggatta ctgaaagata ctttttgcca
     6781 gaaaaaaatc ctcatacatg atgaggataa agtcttaaag gtccattcta tggagtgtcg
     6841 gattgaaaaa tttctccgga tgatatcctt cacagtttaa atgtgatttc aaccataagt
     6901 tttttatgga ttttgttaaa taaaaaagaa ttgagtcgtg tttctgatat gtcttaatat
     6961 aataagtgaa cagttataaa ataattgtgt tatatttatt gtgagtttga tgttatgttc
     7021 tgcataaata actgtagttt agaatattat tcttttatat ttatagctgt gagatgataa
     7081 attataagtg ttctttgtgc tataaagaac cccccgtata agggggggaa agatgggttt
     7141 acttcttttt agtccatgcc ctggtgagct acatgaagct cgacactaca gtaaaaattt
     7201 ggcagttttt ttgaacttta cgcaagcaat ttatttacat tgtaaagaaa accccctcaa
     7261 ttccggaggg ggagtgtagg gaagtgcctg atttatttta tgtacgcgca aaaatgagat
     7321 agcttttatt agatatcatc agccctgtct gcatcagtca gctccttaca gcgtcaggag
     7381 tactgatgaa ggggggaacg ttcctgcgtt tttgcaaagg caatccggct agaggcttta
     7441 ctacgcagaa acattccgga caacttttta ccccagtgta acctgtagat caaccagatt
     7501 ttgttgcagt aaaaatacag tggtttcgca tttgtaatat ttgtcacaaa aataacatca
     7561 atatgttggt tttctgttga taaatggttt ggtcgctgat tatgatttcc ttgtactcca
     7621 caggagtaca gtcatgtgca tactaaaatc cgtgttttcg ataaaattaa ggccgtgata
     7681 cagaatcacg gcttttttct tgcctgaaag gatcatttac gctagaagag ttatgagcgc
     7741 gtctctggtg tttgttgcag ggataatatc taatatgggg caccggaaac gcgctctccc
     7801 ttcgtataca gaatgcccat taatttcttc ttcctgattt tccagacata tattcttgtg
     7861 taaattaaaa aacagagcgt gtttctggtg tgaatttgtt ggctacaatg tgcattgttg
     7921 ttctgtgttc cagaaacacg cttctttttt gagatgcaca tcagtctgat acaaagaggg
     7981 atggttgtat ctgtgtcaga gttttgttat ttttttgtat gctcagttat tgatggttaa
     8041 taatatcaag aaaatcagca agttccttac gctacccctc ttcagtagag ggtttttttt
     8101 atctggtacg ccgtaaaacc cggtccttca gggcgtagag gatgtcaaaa atttcttcga
     8161 aaggtaatac ccgatatccc gaaggaataa caacgaagag gatacagata aagacataat
     8221 acggataagt catcaggagg aagtatgtct tgggtgaaac tgacggaagc agaacgtaag
     8281 tgtagttgcg atgattatgc tgcacaggtt gcacagttaa agttgtccgg cagagataaa
     8341 ttgccacggg aagttagaga aaagtgcggg aaaatgtctt aagaatgatt ctggaagaac
     8401 gaaaagtaag aagcgcaaag gctatgaggg caaaagagta tcgtgccgca gcgaatactt
     8461 ttacctggca gcctgtacgg cgcaggtgaa tagacattcc cctgactcat cctgctcccc
     8521 ttggcggcga aggggattaa tccctatcct gtaacttcta ttttgtggaa tcgaaaacag
     8581 ccgaaaattg tgaaaaaaaa ccgtttcagt cgtctcagta aggaaattcg cttgtgattc
     8641 acctggatgc ataggtatgc ataaggttaa aatttatcag gcgcgatcgc ggtagttttt
     8701 cgggggggtt gttgccattt ttacctgtct gctgccgtga ttgcgctgaa tgcatttcag
     8761 cggtgcgtat aattaaggga ttatggtaaa tccacttact gtcttccctc gtagccatcg
     8821 agataaaccg cagaaatcgt ctcagccagc agcctggatt ttttcggggt aaggtctttg
     8881 ctgcggtaat cagtacccgc aaattgagtt gtgccccgga gtgagtttaa aaattcctgg
     8941 ctgtgaaact gctgaatgcg ttgctgcaca tcactccgtg attttcctgt cgctttatcc
     9001 tttgctccca tatcccagaa ctgccggaaa aaaggcacca tcattcgtgt tgtgtcggcc
     9061 ttctgggtgg aaaagttagg atcaaaacca tcgtctgatg cataaacgta atgccgggta
     9121 tgttgttcca cactgccctt tggcgtgcag gctgtcagga ccgttgccgc tgtcagcagg
     9181 gcgataagat acagtgtgcc gccggaacgt ctcatgagtg tctccttacc tttaaatctt
     9241 tgtactcagt gacttatatt taaccgctga ctatcttatc atcacttgca gccggtgctc
     9301 accggctgaa cagtctggcc cagaatcccc gttttttttc ctgctccagt gcctgcctga
     9361 gctgctctat ctctttatgt agctgttccc gttcggcttc
//
