LOCUS       NZ_CP100514             6785 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Klebsiella pneumoniae strain LH50-a plasmid pLH50-a-C, complete
            sequence.
ACCESSION   NZ_CP100514
VERSION     NZ_CP100514.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499316
            Assembly: GCF_024224875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:32:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,372
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 51 of 105
            Pseudo Genes (incomplete)         :: 48 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 12 of 105
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100514.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="LH50-a"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:573"
                     /plasmid="pLH50-a-C"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            220..411
                     /locus_tag="NLZ12_RS26800"
                     /old_locus_tag="NLZ12_26800"
     CDS             220..411
                     /locus_tag="NLZ12_RS26800"
                     /old_locus_tag="NLZ12_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_254541419.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVESDLCEKLHDDA
                     EHLFRTLFSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            411..833
                     /locus_tag="NLZ12_RS26805"
                     /old_locus_tag="NLZ12_26805"
     CDS             411..833
                     /locus_tag="NLZ12_RS26805"
                     /old_locus_tag="NLZ12_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060627039.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2277..2579)
                     /locus_tag="NLZ12_RS26810"
                     /old_locus_tag="NLZ12_26810"
     CDS             complement(2277..2579)
                     /locus_tag="NLZ12_RS26810"
                     /old_locus_tag="NLZ12_26810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128316336.1"
                     /translation="MKIGSTREYGATKSHLLLMITTLPAGVGVFIYPIAEAVWGELPLL
                     KVVGYTALSLMIGGVVGNIVVRGLFSTSLGILFINPLAMISSLTGIAFLIVQYLP"
                     /name="hypothetical protein"
     gene            complement(2649..3089)
                     /locus_tag="NLZ12_RS26815"
                     /old_locus_tag="NLZ12_26815"
     CDS             complement(2649..3089)
                     /locus_tag="NLZ12_RS26815"
                     /old_locus_tag="NLZ12_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019704629.1"
                     /translation="MSKYLITFDLDTTKLKTLYHGKNYNNAYYDIRSTLSRRGFENIQG
                     SVYLGQEGISEAHGTIAIQELTAKYSWFYECVSNIKFYRIESDLNAQFIADGVNEARKR
                     FYTQLEDMRKSLLSVGLTVEQVNEIIGQQQFKLESTISPDKM"
                     /name="hypothetical protein"
     gene            3851..4042
                     /locus_tag="NLZ12_RS26820"
                     /old_locus_tag="NLZ12_26820"
     CDS             3851..4042
                     /locus_tag="NLZ12_RS26820"
                     /old_locus_tag="NLZ12_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_254541418.1"
                     /translation="MTRQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4042..4443
                     /locus_tag="NLZ12_RS26825"
                     /old_locus_tag="NLZ12_26825"
     CDS             4042..4443
                     /locus_tag="NLZ12_RS26825"
                     /old_locus_tag="NLZ12_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5174..5584)
                     /locus_tag="NLZ12_RS26830"
                     /old_locus_tag="NLZ12_26830"
     CDS             complement(5174..5584)
                     /locus_tag="NLZ12_RS26830"
                     /old_locus_tag="NLZ12_26830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121560301.1"
                     /translation="MGWFGGKKDDSPSVSVRVYTTSTNTNTDTSSSSSSTNIAGDAFWE
                     RIDDLIEQKVNNNYKPKTKKEMDEESDRNIKKEAISIIKWTLIITLSGVVITFMYCTYQ
                     GKDIPEFAKTIFTVASTLAAGAGAFVFGNKSK"
                     /name="hypothetical protein"
     gene            complement(5584..6255)
                     /locus_tag="NLZ12_RS26835"
                     /old_locus_tag="NLZ12_26835"
     CDS             complement(5584..6255)
                     /locus_tag="NLZ12_RS26835"
                     /old_locus_tag="NLZ12_26835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121560299.1"
                     /translation="MKSPHLISTQAAFFFEDTINRPDKLFNKINSDIGELIDSMPETIN
                     LPPIAPADIPRVVGHSSNGEYELSVSLNRVDIKKQYADPLLDQGSIEKFRAICKLIILS
                     VSSDMGISRIGLVSNLYIESSNPSLEISRKYLKKEDPNLLEINLRTNKESKEFGLSFNN
                     IILIQMGNVQSPSYNGKAIIVQVDTNTIETGGCIQKDIITDIFIKKSSIYNPATILERI
                     Q"
                     /name="hypothetical protein"
     Typing          complement(3334..3803)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.212"
                     /coverage="92.0"
                     /evalue="2.75e-165"
     Typing          complement(6477..6785)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.097"
                     /coverage="99.0"
                     /evalue="6.14e-146"
ORIGIN
        1 gtttctgtga tgcttgtcag gggggcggag cctatggaaa aacggccgca cagcggcctt
       61 tccttttccc cgtcacgcga tcgctcggtt ttctcgagtt tttacacgca tgctcctgcc
      121 gctcgccgca gccgaacggc aggagcatag cgactgagtg agcgaggaag cggaaaagag
      181 aacatcccta cactgagcac tgacgctctg aggcctctta tgacgaaaca acagaaaaca
      241 gcgctgaaca tggctaaatt catccagaat cagtctctgc tcctgctgga gaagcttaac
      301 gagcttgatc tcgatgtcga gtccgacctt tgtgagaaac tccacgatga tgcggagcat
      361 cttttccgta cactgttctc gagactggat gaattacagg atggtaactg atggccagag
      421 tgtctattag tgaagcggca cggcttgtta aggttagtcg tcctacaatt tacaagatga
      481 ttaacagtgg gaagttaagt tacacctctg ttgtaaagca cgggaaggct attaaagtta
      541 ttgatacttc tgagcttatt agagtgtttg gttctcttga tggtgtaatc gatactgtaa
      601 aatatgatgt aaaaagtgac gcagaatcta caggtattaa cagcgttggt ttacatgatt
      661 tacaacatag aatcgcttta cttgaagctg aaaacgatgg tcttaaaggg gctgttaagg
      721 ctcgagatga gcatattgat tcccttcgtc aggctatgca gcttctggag cacaagcacg
      781 aaccttcttc accatcccat tccccgtggt ggaaattctg gaaaaaatcc tgaccccatc
      841 atgcttcagt tttcctctcc ggccattccg gggagttcct gaaccttcca aaaaatcgcc
      901 agatctctga acgctcctca ccactaaaaa tacccacaca ctacgcaaaa ttaaaatttt
      961 gcagattcat tatttaaaac aattggttaa tgaaaaatta atgtgcagtg atgtgttaca
     1021 aagtgcagat ccaagtcgtg gacgactcta tggcgaatcg tttacgaatt gcccacgatt
     1081 catatgtgaa tcacgcttta ttatcatgtt gttatgatga atcgactctt gattcaattc
     1141 atcaatttgg gcgttttttg ttcatggtga ctcttgattc aattcacgat tcgaatcgcc
     1201 ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac gtgattgccg gccgtcgacg
     1261 aacccgattc ctgagcgtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg
     1321 ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg gaaagaacgc cggaaacagg
     1381 acttaccctg gctgaatagt gtcaatttcc gcgctatgca cttgcgcgca tactcatgca
     1441 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     1501 taccggtttc ccgtcagaaa cgccctgagg ccgttttagc ggtgcgtaca atcagggcct
     1561 tatgttaaat gacatcgaat ggttggggtt actggtccct acagcggagt gagatgagtg
     1621 tttgttagta ctcaaaagtg ccttccaact tgctctgatg ctaattttta tataaccgtt
     1681 tgccgcgaaa gaacattcag tacttatttt ttaatttttt attgttttta tcttgatgta
     1741 atatttaatt gattttattc cgacgttcgc cgacgttcgc cgacgttcgc cgacgttcgc
     1801 cgatgaatct atattatcca tattaccgcg aaatatttac cctcaaacaa aaccttatac
     1861 tctgagtgat ttttggccat caatgcccct tttaactcaa ttgtaattat cattttaaag
     1921 tctcctatca agtactcaaa agtgccttcc aacttgctct gatgctcatt tttattgaac
     1981 cgtttgccgc gaaagaacat tcagtacttt tctaaaaata taaaattatt ttttatcaat
     2041 agattatgtt ttactcttcc ttgggtgagt ttagtgggtt gctgaaattt tatgcccgat
     2101 cagattgctc gtgcattttt gatcgcgatg cgtaggaaac cgctcggtgg ctagtattaa
     2161 aatacggata ttttgtatgt tgccggtgcc tctattgaag ctaagttgaa acgaaggcac
     2221 atctcttcta aaaaaaagcc cgcagtgcgg gcctttcatg cgcgttgggg ttaatgttat
     2281 ggtaagtatt gcacgattaa gaatgctata cctgtaagac tggagatcat tgctagggga
     2341 tttataaaca aaatacccag cgaagttgaa aataatccac gaacaactat gttgccaaca
     2401 acccctccta tcatgaggga taaggcggta tagcctacaa ctttcagtaa cggtagctca
     2461 ccccatacag cttctgctat tgggtagata aagacgccaa ctccagcggg aagtgtggtg
     2521 atcatcaaaa gtaaatgaga tttagttgca ccgtactctc tggtgctacc aatcttcatt
     2581 agatgagtga tgaatgagga tgcccaccat agcgtcatta taagaagaaa aaccattttt
     2641 acttatgctc acattttatc agggcttatc gtgctttcta atttgaattg ttgctgacct
     2701 attatttcgt ttacttgttc tactgttaaa ccaacggata gtaaactttt gcgcatatct
     2761 tcaagttggg tatagaatct ttttctcgct tcgttaacgc catcagcgat aaactgtgcg
     2821 tttaaatcag attctatacg atagaatttt atgttagata cgcattcata gaaccagctg
     2881 tatttagctg ttagctcttg aattgcaatg gttccgtgag cctcacttat accttcctga
     2941 cctaaataaa cgctaccttg tatgttttca aagccgcgtc ggctcaacgt gcttcgaata
     3001 tcgtaataag cattattgta gttcttgccg tgatacaagg tttttaactt tgttgtatca
     3061 aggtcaaatg taatcaggta tttgctcata tttttcctgc gcttattaac ttttagtagc
     3121 cagcttgact agctggaacc ctaaagcgta tcttaccgac gttaaacgaa aaaaaccgcc
     3181 tggggaggcg gttttttcaa aggttaagtc agttggggaa ctgcttaacc gggtaactgg
     3241 cttggtggag cacagatacc aaatactgtc cttccagtgt agccgcagta gggccatcac
     3301 ttcaagactc ttaatatcta aatccacctg gttctgttac caatggctgc tgccagtggc
     3361 gataagtcgc gtctttccgg attggactca agacgatagt taccggataa ggcgcagcag
     3421 tcggactgaa cgggggggtc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     3481 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
     3541 ccgaatggca ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct
     3601 ttatagtcct gtcgggtttc gccaccactg atttgagcgt caaattctgt gatgcttgtc
     3661 aggggggcgg agcctatgga aaaacggccg ctgtgctgcc ttctttctct ccgtgctgtc
     3721 tgtcctatgc cgttatgtgc gcgttgcgac cgctcgccgc agccgaacgg caggagcgta
     3781 gcgactgagt gagcgaggaa gcggaaaaga gactggtttt acactgtgca ctgacgctct
     3841 gaggctcctt atgacgagac aacagaaaac agcgctgaac atggctaaat tcatccagaa
     3901 tcagtctctg ctcctgctgg agaagcttaa cgagcttgat ctcgatgtcg aggccgacct
     3961 ttgtgagaaa ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga
     4021 tgcattacag gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg
     4081 ggtaagagta gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc
     4141 ggtgagcgca atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggccccttt
     4201 gagcaaccga agggtgaaca ccattctgga caggtctctg gacagcgtga tacagcctct
     4261 gctgaccaga gtgaacaggt gatccgtcat ctgaaacagg agattgaaca cctgaacacc
     4321 ttacttttgt ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa
     4381 aatcaccagg caaaattaac tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc
     4441 tgaccccgtc atgcttcagt tttcctctcc ggccatgccg gggagttcct gaaccttccc
     4501 aaaaatcgtc agatctctga acgctcctca ccactaaaaa tacccacacc ccacgcaaaa
     4561 ttaaaatttt gaagataact cattaatatc agttagttgt gggttattaa attacgcagt
     4621 attactttct gtaagatgaa tccaagtcgt cggcgattcg tgtacgactt gccgacgact
     4681 cacttaagac tatcagtaac ttatgacgac ttgactctag agtcgaatcg tcattttggt
     4741 gtttttttga ccttgacgac tctagagtca agtcacgagt cgaatcgccg gtgaatcgtg
     4801 tggtgagtcg tccggcgact tgccataacg tgattgccgg ccgtcgacga acccgattcc
     4861 tgatcgtgct gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa
     4921 attgcgtccc cgttcaccgt tttcggatgg tatgaacgcc ggaaacagga cttaccctgg
     4981 ctgaatggcg tgagttttcg cgctatgcac ttgcgtgcat actcatgcat gccgtaaaaa
     5041 catagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc
     5101 cgtcagaaac gccctgaggc cgtgttagcg gtgcgtacaa ttaagtagtt atgttaaatc
     5161 aacctctcgt tttctacttt gatttattac caaagacaaa cgcaccagca cctgcagcaa
     5221 gtgtgctggc aacagtaaat attgtttttg caaactcagg gatatctttc ccttgatatg
     5281 tgcagtacat gaatgtaata acaaccccac ttagagttat tattaatgtc cattttatta
     5341 tgctgatagc ctcttttttt atgtttctgt cactttcctc atccatttcc ttttttgtct
     5401 ttggtttata gttgttgttt actttttgtt caatgaggtc gtcaatcctc tcccagaacg
     5461 catcacctgc gatatttgtt gatgatgaag aagaagaggt atctgtgttg gtgtttgttg
     5521 atgtggtata tactcttaca gacacagatg gtgagtcatc tttttttcca ccaaaccatc
     5581 ccatcactgt atcctttcta aaattgtagc agggttatat atggatgatt ttttaataaa
     5641 tatgtcagta attatatctt tttgtatgca gccacctgtt tcaatcgtat tggtgtcaac
     5701 ttgcacaatg attgctttgc cgttatagct tggggactgt acatttccca tctgaattaa
     5761 aattatatta ttaaagctca ggccaaattc tttactctct ttattcgtcc ttaggtttat
     5821 ttcaaggagg tttggatctt ctttctttaa atatttcctc gatatttcta aagagggatt
     5881 gctgctttca atgtaaaggt tgctaactaa cccaattcga gatataccca tatctgaaga
     5941 taccgatagt attataagtt tgcatatcgc tctgaatttt tcaattgaac cttggtcgag
     6001 caatgggtcg gcatattgtt tttttatatc cactcgattg agtgatacgc ttaattcata
     6061 ttcaccattt gatgagtgac ccactactct aggaatgtcc gcaggggcta taggaggaag
     6121 gtttattgtc tcaggcattg aatctattaa ttcaccaata tccgagttga ttttattgaa
     6181 taacttatca ggtctgttta ttgtatcttc aaagaaaaaa gctgcttgag tagatattag
     6241 gtgaggggat ttcatttttt acctctattg ttatttgtta atacccttga cgatagcata
     6301 tctgagaggg tagtctccaa tacgaaaaaa ccgccttggc gggcggtttt ttcgaaggtt
     6361 cgaggagttg gcgctctttg aaccgaggta actggcttgg aggagcgcag taaccaaatt
     6421 cgttctttca gtttagcctt aactggcaca taacttcaag actaactcct ctaaatcagt
     6481 taccagtggc tgctgccagt ggcgcttttg catgcctttc cgggttggac tcaagatgac
     6541 agttaccgga taaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct
     6601 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     6661 aacagcggaa tgacaccggt aaaccgaatg gcaggaacag gagagcgcac gagggagcca
     6721 tcagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     6781 cgtca
//
