LOCUS       NZ_CP100493             1506 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Escherichia coli strain LH85-a plasmid pLH85-a-G, complete sequence.
ACCESSION   NZ_CP100493
VERSION     NZ_CP100493.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499321
            Assembly: GCF_024224055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:47:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,168
            CDSs (total)                      :: 5,047
            Genes (coding)                    :: 4,895
            CDSs (with protein)               :: 4,895
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 45 of 152
            Pseudo Genes (incomplete)         :: 114 of 152
            Pseudo Genes (internal stop)      :: 27 of 152
            Pseudo Genes (multiple problems)  :: 30 of 152
            Pseudo Genes (short protein)      :: 3 of 152
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100493.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LH85-a"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pLH85-a-G"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            1..441
                     /locus_tag="NLZ16_RS25770"
                     /old_locus_tag="NLZ16_25765"
     CDS             1..441
                     /locus_tag="NLZ16_RS25770"
                     /old_locus_tag="NLZ16_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          complement(727..983)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="98.0"
                     /evalue="3.06e-107"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagttggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccggattta aagagccagc ggcaggcgga caatcagggc tacgtgttaa
      481 cgttttgacc atgattgtct atcctgcatt gctcttttgc cgcatgtcag ttcgatgcga
      541 tgcaggatag ggcaaacgcc gcaaaatgac gtcattgacg tcattcgcaa cctttgcccg
      601 ataatctcca aaacgtctga gctgccacgg ttccggcagc gccttgagcg ctgccggaaa
      661 ccgtctgggg agaaccccag acccccatcg ctgtaacatc gacggtctac gaccaccaac
      721 cagctgaata gctgccagtg ccgtgcaaat gggctttgca gggggctgac gcggttcctg
      781 gcgacctgaa agcccctggt taggggtatc ggcgcgcctg acctcctgaa accctctgta
      841 agcgtttttc gcgccttcca tgccctgacg ggcatttaaa acccgtttcg tgcgcctgag
      901 agcttctgag agcgtttgaa gagctatctg gcgactcatc cccgaaagaa caacagcgga
      961 atcaccgata acatgcgaga ggtggcaccg ctcgccgcag ccccagcgac cgaacgttag
     1021 tgagcgagtg ggcgaggaag cggaagagaa ccggaaacca cattgagcac gtacgcactg
     1081 atgcggggtg tcggggtgaa gccctgacca gatggcaaac ggaatatcgt cgcgggtgac
     1141 ggtattacat ttgcacatcc tgtcccgatt tctgcggagt ttaacggtga acggacggca
     1201 aaaaacgtgc aggagctgtt gaacgtgaaa acctcgaaat ctggcaaaaa tcacggctga
     1261 cgaggcttgc agaggattaa gccagtatac actccgctat cgctacgtga ctgggtcagg
     1321 gcttcgcccc gacaccccaa agagtagcta ggataaatat ctaatggaat caatgcgtta
     1381 gacgcctaat ataaagatac agaaaggaga ataaaaacat taacaaaatc taagggttac
     1441 agttgacata gttatcaata atgataacac tgttatcatt attgataact caaatagggt
     1501 tcaaag
//
