LOCUS       NZ_CP100485             5962 bp    DNA     circular CON 22-JUL-2023
DEFINITION  Klebsiella grimontii strain LH87-a plasmid pLH87-a-C, complete
            sequence.
ACCESSION   NZ_CP100485
VERSION     NZ_CP100485.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29499322
            Assembly: GCF_024224355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Enterobacteriaceae from poultry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x, 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/22/2023 02:45:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,823
            CDSs (total)                      :: 5,706
            Genes (coding)                    :: 5,631
            CDSs (with protein)               :: 5,631
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 31 of 75
            Pseudo Genes (incomplete)         :: 46 of 75
            Pseudo Genes (internal stop)      :: 13 of 75
            Pseudo Genes (multiple problems)  :: 14 of 75
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100485.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="LH87-a"
                     /isolation_source="pooled poultry feces"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:2058152"
                     /plasmid="pLH87-a-C"
                     /geo_loc_name="Switzerland"
                     /collection_date="2022"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            join(5525..5962,1..66)
                     /locus_tag="NLZ17_RS29050"
                     /old_locus_tag="NLZ17_29050"
     CDS             join(5525..5962,1..66)
                     /locus_tag="NLZ17_RS29050"
                     /old_locus_tag="NLZ17_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254547973.1"
                     /translation="MTKKTAQSSAESVLQAFLSEAAERFKPDATVLATRIETAVQRYTA
                     SSTTQRFHAAAPLAIQQLQERILEGWRYDIGIPQSVYVAGTGNMSVTLRKPAVLMEQEI
                     ADLKRQVEEGYHNELAAAMEREVDKLVQDAANEAQRRAEEAAAAERDAMRQRLRDMLLT
                     GATV"
                     /name="hypothetical protein"
     gene            63..206
                     /locus_tag="NLZ17_RS29055"
                     /old_locus_tag="NLZ17_29055"
     CDS             63..206
                     /locus_tag="NLZ17_RS29055"
                     /old_locus_tag="NLZ17_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180264768.1"
                     /translation="MTVTKKGPPCPAYIDPEALEKAIAAARKIREANPERYKSIPVQPK
                     LF"
                     /name="hypothetical protein"
     gene            335..997
                     /locus_tag="NLZ17_RS29060"
                     /old_locus_tag="NLZ17_29060"
     CDS             335..997
                     /locus_tag="NLZ17_RS29060"
                     /old_locus_tag="NLZ17_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004864809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="WP_135717727.1"
                     /translation="MTHAVTSHYGNGRYHLIHSANYTFNDKEYNLNFKPDSKRYSPIRQ
                     EIMDSFITQLCAMQSHYSKVFAFRFDLSVPAGISVSESNKLISDLFTRLRGKFTAKRWN
                     GQPIKKFAYGWVREKEKAKQVHYHCWIALPHFQVRSTGFGNEGTFGLINTLWGELTVQA
                     GRAHLCDDTYIINRDEHSSLVELVERVSYLAKARGKYSTGGGQRMFSASKLKILTAA"
                     /name="inovirus-type Gp2 protein"
     gene            complement(1221..1952)
                     /locus_tag="NLZ17_RS29065"
                     /old_locus_tag="NLZ17_29065"
     CDS             complement(1221..1952)
                     /locus_tag="NLZ17_RS29065"
                     /old_locus_tag="NLZ17_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516290.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_254547977.1"
                     /translation="MQVVTYRRVSTERQGRSGLGLEAQADYIAHAVRQHGWEIIGEFTD
                     EGMSGSLAPMQRDGLRQAIELCRLTGACLLVAKLDRLSRSVLDIATLVEELPCIKVATM
                     PMADKFQLHLYAALAEQERDFIKQRTSDALASLKARAAAGDELSQQKVQTWSDNIAAAH
                     ASGINRQRSIKVRAARADAFAATVEDNILAARTRGHTTLQAIANYLNERGITTRRGKRW
                     TPAAVSRVESRFPTPPDRSSM"
                     /name="recombinase family protein"
     gene            complement(2583..3050)
                     /locus_tag="NLZ17_RS29070"
                     /old_locus_tag="NLZ17_29070"
     CDS             complement(2583..3050)
                     /locus_tag="NLZ17_RS29070"
                     /old_locus_tag="NLZ17_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011899450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254547974.1"
                     /translation="MPAPNNYGVAGSPNSAIAHPDGLDCYGRVPEINSKGDVIPAGDIF
                     LRVGKHTGPNRGFGVRHIWAEHEQELVRLGYATVNDVARFVRDIIRRGAPIYCEFNHPG
                     GRHRPTVLKSAVGIVVLEPREAPETDSGWIYVVVTAYTRRTAHGVLVGHVV"
                     /name="hypothetical protein"
     gene            4154..4438
                     /locus_tag="NLZ17_RS29075"
                     /old_locus_tag="NLZ17_29075"
     CDS             4154..4438
                     /locus_tag="NLZ17_RS29075"
                     /old_locus_tag="NLZ17_29075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161658354.1"
                     /translation="MTNEELQQILAANNQMMLNTFNTMLTQQTQQFEQLLGQAKTVFYT
                     QGYNVGSAHGYVTAIDALKPAISDGLRHGSPECGKAMQSFRSLGLEDEE"
                     /name="hypothetical protein"
     gene            complement(4464..4913)
                     /locus_tag="NLZ17_RS29080"
                     /old_locus_tag="NLZ17_29080"
     CDS             complement(4464..4913)
                     /locus_tag="NLZ17_RS29080"
                     /old_locus_tag="NLZ17_29080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254547972.1"
                     /translation="MSKQSQNLLNLTQAADAVGITRKTLYSHIDKGLVSVTRQQGKRYV
                     DVSELIRHYGSVSLPDEKVNTVTRSQTKHDDDAISAMQRELVELRKLIEKQQLLLEDKQ
                     ETDRLREQMTGLQNERDAAAQELEKVKSELEAERKKGFLKRLFLK"
                     /name="hypothetical protein"
ORIGIN
        1 gcagcagagc gtgatgctat gcgccaacgc ctccgcgata tgctcctgac aggggccact
       61 gtatgaccgt caccaaaaag gggccaccat gccccgctta catcgacccg gaggccttgg
      121 aaaaggcgat cgccgccgct cgaaaaatcc gggaagccaa cccggaacgc tacaaatcca
      181 tccccgtcca gccgaaactt ttctgagtaa aaacgctggc aataattgca tctccgcgtc
      241 agcagtggcg cggcataggc agactttgct aaagcagtaa ccgtactggt tcttattaat
      301 acctgtacat acccaaccat caaccggagg aacattgact catgcagtaa ccagccatta
      361 tggcaacggc agatatcatc ttatccactc agccaactac accttcaacg acaaagaata
      421 caacctgaat ttcaaacctg acagcaagcg ctactcgcca atccgtcagg agataatgga
      481 cagcttcatt acccaactct gcgcgatgca gtcccattac agcaaggtct tcgccttccg
      541 tttcgacctg tcagtcccgg caggaatttc cgtatcagaa agcaacaaat taatcagcga
      601 cctgtttacc cggttgcgtg gcaagttcac cgccaagcgc tggaatggac agccgatcaa
      661 gaaatttgca tatggctggg tcagggaaaa agagaaggct aaacaggttc attaccattg
      721 ctggatcgca ttacctcact ttcaagtcag atctaccggt ttcggcaacg agggcacatt
      781 cggactgata aacactcttt ggggtgaact aaccgtacag gctggtcgcg cccacttatg
      841 tgatgataca tacatcataa acagggatga acactcatcg ctggtggaac tggttgagcg
      901 tgtctcctac ctcgctaaag cgcgtgggaa atattctaca gggggcgggc agcgtatgtt
      961 ttctgcatca aagttgaaaa tactgactgc tgcttaaccg ggttcgaccc cacctggtca
     1021 gccttacgca cacgaaaaat ttctacaagg catattccca tgacagattt gtcgccagca
     1081 ggcacaccaa cgcccacagc gatccacgca ccgccggttt tctcacatta aatatactta
     1141 aacttttgtt atttaataaa aaataaagcc acgcacctcg gcgaggctaa tttatacgat
     1201 acagggagta cgtctacagg tcacattgac gatctgtctg gtggagtggg aaaccggctc
     1261 tcgacccggc tcactgccgc aggtgtccaa cgtttaccgc gccgggtagt gatcccgcgc
     1321 tcatttaaat aattagcgat agcctggaga gtggtatggc cgcgtgtccg ggctgcgaga
     1381 atattatcct cgacggtcgc cgcgaaagca tcggcccgag cggctctgac cttgatacta
     1441 cgctgacggt ttatcccact ggcgtgtgca gccgcaatat tgtcactcca cgtttgcacc
     1501 ttttgttgtg acagttcatc acccgccgca gccctcgcct ttaacgacgc cagcgcatca
     1561 ctggtacgct gtttgataaa atcccgctcc tgttcagcta acgccgcata caaatggagc
     1621 tgaaacttat ctgccatagg catagtagca accttgatgc agggcaactc ctccaccagc
     1681 gtggcgatat ccaaaactga ccgactcagg cgatccagtt tagccaccag caaacacgcc
     1741 ccggtgagcc tgcacaattc tattgcctga cgcagcccat cgcgctgcat tggagccaga
     1801 gaaccactca taccctcatc ggtgaactca ccaatgatct cccaaccatg ttggcggaca
     1861 gcgtgagcga tgtagtcggc ctgagcctca agccccaaac cagagcgacc ctgacgctca
     1921 gtagacacgc gacgatacgt aacaacctgc atattcatcc ctcccaaata accgtggaca
     1981 caccttatca taagtttact gaacggaaaa caaatgataa aaccgatcaa caagagagaa
     2041 aaacaacata atccattaat tattaaagga tatttgaaaa tcactatcac tgacttttat
     2101 catccagcga catcggtcag gcagatctcc aagtgcgcca gccgtacaat caccggcaag
     2161 atatttgcag ccagccggtt tacatatggt ggggggtatc agcgatccag ccgcaggggc
     2221 gggggtgtat ctgcccagcg acatgggggt cagaatatgc agttagaata tagggtctct
     2281 ttagtcggtg atttcaagaa acccagacac cgcaagggat tgcagggata tagagaaaag
     2341 ttagtatata ggtaacactc aacctttagt tgaatatctt cggtaatgcg tatagaatta
     2401 ttttactgca agagcattcg cagggggctt caaccagtaa ttggccttct gtgccgggca
     2461 gtcagatgac tggtcatctg ccgggtagtt acgacaagag tcggtttctg aaccctatgt
     2521 gctaaggaaa tgctgcgact accaacaaat ttctaagggg cgcttttgag cgccctattt
     2581 ttttatacaa catgaccaac caagacgccg tgcgccgtcc ttcgtgtgta cgcagtcaca
     2641 acgacataga tccaacctga atcagtttca ggcgcttctc ttggctcaag aaccacaata
     2701 ccaaccgcag actttaacac cgtagggcga tgcctaccgc cgggatgatt aaactcgcaa
     2761 tagataggcg cgccacgacg aataatgtcc cggacaaaac gcgccacatc attaacagtc
     2821 gcatatccca atcgaaccag ttcctgttca tgctcagccc agatatggcg aacaccgaac
     2881 ccacggttag gacctgtatg cttaccaaca cgcaagaaga tatcaccggc aggaatgaca
     2941 tcacctttag aattaatttc agggacacga ccatagcaat caagtccatc cgggtgagct
     3001 atagcgctgt taggtgaacc ggccacaccg taattatttg gagcaggcac aaaacgatcc
     3061 ttatcgagtg gagtccgcac attctaccag agaagaaaca agcggaagga acgccagaaa
     3121 aaagaaaggc aggccttttc caatagctcc gctactcctg acgagcatca caaaaatgac
     3181 gctgcaagcc agaagtagcg aaaccgtgcc agctttgaga gtcggcgcgt ttccttgccg
     3241 tgatcccctg attgcgtcct gacatcccac catgcacttt tactgtcatc aagccgcctt
     3301 tttcccttgc gggacgcgtg gcgttttctc attcctcacg ctatctgatg atcggttttc
     3361 tgtttagatg gtcgctccaa cgctgcgggt attaatcaga ctccccgtct cccgctgctg
     3421 ttaccgcctg actatgacaa actcatgctt ccagttcgtc aggcacggaa aaacgccgct
     3481 aatggcaaca gccagtgcgg tagcgtgtcc tattggggga tttcgtcggt aggtcttgaa
     3541 gtgattcccg agagtgggct acactgagat tactgatttg gtgacgctgt tcccccgaac
     3601 acgttacctg gttaatcagt cctgagaaaa ctgattagcc cgtcaaaacc cacgcctgag
     3661 aacgtgggtt ttttcgtttc tgagcattac cttaccccgt tatagggctg gctggaagat
     3721 cccgccgcca aattttaccg acgagcctta actgccttcg ctttctttgg gcatgttaag
     3781 gcaggggcct ttgctacttc cgaagaaaac accaagctaa cccgatcggc tttgaatcca
     3841 tacgcatcga cattacacgc actgaaccca gccccacgta ctgcggctaa acaaatcgcg
     3901 actgatagca aaagagctat cgtccatttc aatacagttt taatcatatc tcgctccttg
     3961 agatggttgg ttgaactgta tttatttatt cgccaacagc agatcacggt gccaaaaggc
     4021 gtaaaaaatg gctttttcac cctcaacgta aaagaatgct aattaagttg cgtaacgaat
     4081 tttatcaggt aaaacaaaca taacagcggc atggtttctg acgctggcag agcaatccga
     4141 aggggagaaa aagatgacaa acgaagaact ccaacaaatt ctggctgcga ataaccagat
     4201 gatgttgaat actttcaata cgatgctgac gcaacagact cagcagtttg aacaactgct
     4261 gggacaggca aaaaccgttt tttacactca ggggtacaat gttggttcgg ctcatggata
     4321 tgtgactgcc attgatgctt taaaaccagc catttctgac gggttgcgtc acggctcacc
     4381 tgagtgcggg aaagcaatgc agtcttttcg tagcctcggt cttgaagacg aagagtaagt
     4441 gcaatccgcc tgaaaaggcg gatttatttc aaaaacagtc gttttaaaaa acccttttta
     4501 cgctccgctt caagctcaga cttaactttt tccagctcct gagccgctgc atcacgctca
     4561 ttttgcaacc cggtcatctg ttcccgcagc cggtcggtct cctgcttatc ctcaagcaat
     4621 aattgttgct tctcgattag cttcctcagc tctaccagct cccgctgcat agccgatatt
     4681 gcatcgtcat catgtttagt ttgtgaccgt gtaacggtgt ttactttttc atctggcaag
     4741 gatacagatc cgtagtgacg gataagctcc gacacatcta catatcgttt accttgttgc
     4801 cgagtaacac tcaccaaccc cttatcaata tgggaataaa gtgtcttcct cgtgatacca
     4861 actgcatccg cagcctgtgt aaggttgagt agattctggc tctgtttact cactgtatac
     4921 ccgccataaa attgatgtgt ttactctgga tagtatacac aaaaacaaat ccataaaagt
     4981 taatgataat tattctcaaa tgaagaattt catgataaaa tagaatctga ccaataaacc
     5041 acacaagaca ggagaaatat tgaccttaac gcccacgctg ccaacgtttg cagccccgac
     5101 cgatacggaa cgcgccaccc tcgccgcgat cctgaataat actggcctcc gcgccacagc
     5161 cccccggatt aacgtcttac attacctgaa cgcaaccgac actcctgtaa ccgctgagat
     5221 gatcagccgc ttcgttgacc tcccgttatc gactgcgtac agaacgctca cagcactgga
     5281 aataacccac ctcgcagggg ttactttcgg acgcggagac gttactcgct ggtttcgatt
     5341 ctgccctgag acaccgcagc attgccccgc gtgtggacag agttactacg gcgaatattc
     5401 ctgaccacct gatacacaaa cactgacgag atgaccgaac ctaacggtca caccaaatga
     5461 cgcccacaga gagcgccagc agcccgttac ggcctttact caatgattaa agagaattac
     5521 ccccatgaca aagaagacag cacagagctc cgctgagagc gttttacagg cattcttgag
     5581 cgaagccgca gaacggttca aacccgatgc aaccgtatta gccacgcgga tcgaaacagc
     5641 cgtccaacgg tacaccgcca gcagcaccac acaacgtttc cacgccgccg caccgctggc
     5701 aatccagcaa ctacaggaga ggatattgga aggctggaga tacgatatcg gcatcccaca
     5761 gtccgtttac gttgccggta cggggaatat gagtgttacg cttcgcaaac cggcggtgct
     5821 gatggaacag gagatcgccg acctaaaacg acaggtggaa gagggctatc acaacgaact
     5881 cgccgccgcg atggagcgtg aggtcgataa actggttcag gatgccgcta acgaggcaca
     5941 gcgtcgagca gaggaagccg ca
//
