LOCUS       NZ_CP100124             2058 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain GZKP13 plasmid pGZKP13-4, complete
            sequence.
ACCESSION   NZ_CP100124
VERSION     NZ_CP100124.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388304
            Assembly: GCF_025884275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:03:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,541
            Genes (coding)                    :: 5,362
            CDSs (with protein)               :: 5,362
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 69 of 179
            Pseudo Genes (incomplete)         :: 109 of 179
            Pseudo Genes (internal stop)      :: 33 of 179
            Pseudo Genes (multiple problems)  :: 28 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100124.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GZKP13"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pGZKP13-4"
                     /geo_loc_name="China"
                     /lat_lon="23.06 N 113.15 E"
                     /collection_date="2018"
                     /collected_by="The First Affiliated Hospital of Guangzhou
                     Medical University"
     gene            492..683
                     /locus_tag="NIK98_RS28290"
                     /old_locus_tag="NIK98_28265"
     CDS             492..683
                     /locus_tag="NIK98_RS28290"
                     /old_locus_tag="NIK98_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020324555.1"
                     /translation="MIVRLDNRLFEILDEMGAHVDREMFMAAYAQRKAEKENDPRHIRQ
                     RKLTRLAHDLMSESDPEL"
                     /name="hypothetical protein"
     gene            1007..1996
                     /locus_tag="NIK98_RS28295"
                     /old_locus_tag="NIK98_28270"
     CDS             1007..1996
                     /locus_tag="NIK98_RS28295"
                     /old_locus_tag="NIK98_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_040207214.1"
                     /translation="MQNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIEELGETLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPKWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVKKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          complement(1723..1914)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 gtaccaaagt cacatcttgt ccgattgatt ttactccgat gcaggatagg gctaacgccg
       61 caaagtgacg tcactcgatt actgagttcg gattccgaac ctgggttttt cttccggtaa
      121 ttttccggaa ggcgcgctta gtagtttctc atctggcgat gagaaacgcg cggtggggca
      181 agcccacgaa aaacaaaaac tgataacgga caagatgtgt ttttggagta ccgccatcac
      241 gatgccggta ccaaaaacga tccgtcaggg ctgcgccccg acaccctgcc aacacctcac
      301 gccgccagat taaaatctgt cagcgttcgc cgttcccgaa atcgtcaaac gctaaatcgc
      361 ggggtcccgc tggcttgcca gtgggttccg atttagtgat tgacgacagc acccgtaaaa
      421 tacttacagg gggtggttgt gatcacgcag tgagcgcaac cacctgataa gcgttttttg
      481 agggggtagg catgatcgtc agactggata accggctgtt tgagattctc gatgagatgg
      541 gtgcgcatgt ggatcgggaa atgttcatgg cggcctatgc gcagcggaag gccgaaaaag
      601 aaaatgatcc ccggcatatc cgtcaacgga aactgaccag gttggctcat gatttgatgt
      661 cagaatcgga ccctgaattg tgagcctcgc gtgcgcgcga gggaaatcgg tcagaaaatg
      721 gccggagtct cgtgcgcgcg atccttgtat ttatacttaa gggataactc tggcgtcaaa
      781 aaacgccgcc agcccttgtg ccgcaagggt ttgagtgtga tttgcctgaa aatggttttg
      841 gaagtattct gtcgggagtg gtcgcagaaa aaagcgaccc ccgcaactgc gctaacagtt
      901 cgggggtgtg accacctgta ggaattcgaa tttggacacg acaacacagg tgatatggca
      961 atgataacac gtcaaatgag ccatcgccca gcatggggcg gtgtgcatgc aaaatcctga
     1021 actccagaac ctaactgact actccccctc agatgcgccg tgggacgctc acagaagcgt
     1081 ttctgacgac gtgggaggga tttacctgct tgcggctgaa tatgagcgct acggggcgcg
     1141 tatggcctcc tgtggcggtt tactgaggtt tgggtggtct accctcaaag aaaccggaga
     1201 aacgcgcctg aggctgcgtg aggcgcattt ctgccgtgtc cggcactgtc ctgtctgcca
     1261 gtggaggcgc tccctgatgt ggcaagcccg tttctaccag tccctgccgc gtatcgtggc
     1321 ggattacccc gattcccgct ggatgtttct gaccctgacc gtgcgcaact gcgcgatcga
     1381 ggagctgggc gagacgctga gccggatgaa cacggctttc cagcgactga aggaccgtaa
     1441 agagttccga ccagttcagg gctggatacg caccacggaa gtcacccgcg gcagtgacgg
     1501 cagcgcacac ccgcacttcc acaccctgat gatggttccg ccgggcatgc ttaacgggaa
     1561 gagttacgtc aggcatgaac gctgggttga gctgtggcgt gagtgcctgc gggtcagtta
     1621 cgatccgaat gtcgatgtcc gggcagtgaa accccgcaag ccgaaggacg gcgaatcgct
     1681 ggcctgcgcg accgccgaac tggtgcgcgg cgcagtggcc gaaaccctga aatattccac
     1741 gaagccggcc gacatggtgg ccgatcctaa atggttcctc gaactgaccc gccagacgca
     1801 caagcgccgc tttgtggcga ccggcggcgc actcaaagac gtgctgaaac tcgatcagga
     1861 aaccgacgcc gacatggtga tcggggatga tatttccgag ggcgatgatg acggttcccg
     1921 catcgcgttc gagtggaaga ccgaggtcaa aaagtaccgg cgttcgccga cgaaagataa
     1981 aaccgaatca gactaacagg gtgtcggggc tgcgccctga ccatgagggc tttgggcatc
     2041 cgtcaccttg gtggcggt
//
