LOCUS       NZ_CP100119             2953 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain HSKP1 plasmid pHSKP1-5, complete
            sequence.
ACCESSION   NZ_CP100119
VERSION     NZ_CP100119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388305
            Assembly: GCF_025884255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:04:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,412
            Genes (coding)                    :: 5,235
            CDSs (with protein)               :: 5,235
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 71 of 177
            Pseudo Genes (incomplete)         :: 115 of 177
            Pseudo Genes (internal stop)      :: 25 of 177
            Pseudo Genes (multiple problems)  :: 31 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100119.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HSKP1"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pHSKP1-5"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Huashan Hospital"
     gene            join(2445..2953,1..466)
                     /gene="mobV"
                     /locus_tag="NIL09_RS27625"
                     /old_locus_tag="NIL09_27610"
     CDS             join(2445..2953,1..466)
                     /gene="mobV"
                     /locus_tag="NIL09_RS27625"
                     /old_locus_tag="NIL09_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            complement(493..843)
                     /locus_tag="NIL09_RS27630"
                     /old_locus_tag="NIL09_27615"
     CDS             complement(493..843)
                     /locus_tag="NIL09_RS27630"
                     /old_locus_tag="NIL09_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(840..1391)
                     /locus_tag="NIL09_RS27635"
                     /old_locus_tag="NIL09_27620"
     CDS             complement(840..1391)
                     /locus_tag="NIL09_RS27635"
                     /old_locus_tag="NIL09_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     Typing          2452..2951
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.24"
                     /coverage="99.0"
                     /evalue="9.51e-86"
ORIGIN
        1 catcgagggc agccgggcca cccatcagcg cgtccagagc tactacggcg ccatcgagcg
       61 ccagcccggg cacgccacca tcacgccgca ggccatcgag ccgcgggtgc tacgcaaggg
      121 gattttctcg aaggacgtgg agacgccgga ggccgtggcc gcccgcgtga ccgctgccgt
      181 cagggaaggg tatggcccga ccgtcgcagc ggccgcagga gcgcgtcagg agcgcgagaa
      241 ggcccggcag gcacaggaga cagcccggag cctgcgtgac cgcctgaagc ccgttctgga
      301 cgctctgggg ccgcttaatc gcgatatgca ggccaaggcg gcccagatca tcaaggccgt
      361 gggcgagaag ctgctcgccg aacagcgcga ggcgacacgc cagcggcagg cccaacggct
      421 tcgcagtcgc ggccgggatc gcgacgacgg cgggctgtct cgttgaccct acaggtggcc
      481 gacggaaggg gtttatggct tgcagctact gaggacggtt tgctccttcc cgccatcgtg
      541 ggtcgaaagg ttcatgtcgt tgcccttacc ccacagttcg taggtgtagt ttttgctagc
      601 ggagatgtag cgcactcctg atgcgctctt ggcgcgtttc agtgggacaa gctcatccat
      661 ttgcagcatc acggcatagt ctccgacgta cacgacatcc aacgtctttc cgccttcgca
      721 agcgtacttg gtctggatca tctcatccgc tgctgaagca agattcggag cgattgccgc
      781 catcatggca gcaagagcga acgttttttt cataaagatt ttctccttcg tttcgttgct
      841 cattggggag tgctttcaaa catgtcaatg gtgttcgggt cgcggtcgtc gttctgcttc
      901 tcgccgctga acatcgacat gatccggcgg cggatatcgc catccttgtt gaccaaggtt
      961 ttggtcacga aagctgcgta atccttggcg ttgtcgtagc ccttccagac ctcgtgagga
     1021 tccagcgcat aggcgcaaac cttgccagca acagggatgc ggacaagcgc gcgattttct
     1081 tccaggtaag acaaccaacg gctgattgtc cgcctgttga cgttcatctt gtctgccaaa
     1141 aactgctgat cgcagaccac cgcgccgcag gttgggtcga tattttcagc aaagaatgaa
     1201 tacaggccga aggccccagc attgccttta gcaagctcaa tgatgcgctt ccaacccaag
     1261 ggatacacct gcgtgaaccc cttgttcttc cgcgcctctt cgcgctcctg agcctctttc
     1321 tcgtccagca tggccagctc tcgctggcgg atacgacgct gggcgtcggt cgccaccgtg
     1381 atctgctgca tggggttcct ccaaagtcgg gaaatttgtc cgctcattgc gccatttcga
     1441 gtcgcaatga tacgacaaat tatgtctcac gtcgagacag tttgcgtctc aaccttgcta
     1501 cctgttgaga catccatgca tgtctcaacg ttaggacacc ctatgtgtct caatgcattt
     1561 ctacaaatcc ttataaatca ataacttagc atttccccct ttctcctcta aggggctgcc
     1621 gccccgccgg cggccgctac gcggccttgg caccccgctg acggcccctg cggggcctct
     1681 ggaaaaggcc cacccggcca ccggccgggt gacgttgttt ggggggcctc cggcggccgc
     1741 tgcgcagcct tggggcggtg ggcgctgccc cccgccaccc cccgcgttcg ccacgtggca
     1801 ggggtgcagg ggctacgggc ctgcggccct tcactcctgc acccctaccg gggagcgtgg
     1861 ggtcgttcca ggcgcgtcaa gggccgctgc gctctccggg ggtggcccgt gtcctcgccc
     1921 tgcgggctgc gggccgcgcc agccaccccc tgcgcccttg acccgccttc cactccggcg
     1981 ctgtcggaaa tttcctaccc ggccatgagg cgatttccga cacgccagcg ggccactggc
     2041 tgacgccttc agcggagggc agggtcaagg gcgcgcccgc aggccgcagc ccggagcgaa
     2101 gcgcagggac cgggcgagga cgcgggcgaa gccgaaccct tgacgctgac cgcagcgccc
     2161 cagcctgtgc gagtgggggc agggacgaag ggcgaagccc gccacccctg ccccccgagc
     2221 ctcccggcgg cgagggtggg gagtccagag gggcaaagcc cctttgggca aggccgaagg
     2281 ccgccgcagg agcaagcccg aagggcgctg caaccgccgc aggcgggcac gactttgctt
     2341 cgcaaagtct agtgagtaca ctatcatctg atagtggccc gccggaggca ccgccttgcg
     2401 ctgccccgac tggccggatt cggacaccaa ggggaggggc aggcatggcg gcctacgcga
     2461 tcatgcgatg caagaagctg gcgacgatgg gcagcgtggc ggcggcgttg cagcactgct
     2521 accgcgagcg cgaaacgccc aacgctgacg ccgaacgcac gccggacaac gagcaccggg
     2581 cggccacctc aaccgatgcg gcgatggggc ggctgcgcga gttgctgccg gagaagcggc
     2641 gcaaggacgc ggtgctggcg gtcgaatacg tgatgacggc cagcccggaa tggtgggcca
     2701 aggccacgcc ccagcagcag gccgcgttct tcgaccaggc gcagggctgg ctggcggcca
     2761 aatacggcgc ggatcgcatc gtgacggcca gcatccaccg cgacgaggcc accccgcacc
     2821 tgtcggcctt cgtcgtgccg ctgacgcagg acgggcggct gtcggccaag gagttcatcg
     2881 gtggccgcga caagatgcgg gccgaccaga ccagctttgc cgaggcggtg agggatctcg
     2941 ggctggaacg cgg
//
