LOCUS       NZ_CP100118             5596 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain HSKP1 plasmid pHSKP1-4, complete
            sequence.
ACCESSION   NZ_CP100118
VERSION     NZ_CP100118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388305
            Assembly: GCF_025884255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:04:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,412
            Genes (coding)                    :: 5,235
            CDSs (with protein)               :: 5,235
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 71 of 177
            Pseudo Genes (incomplete)         :: 115 of 177
            Pseudo Genes (internal stop)      :: 25 of 177
            Pseudo Genes (multiple problems)  :: 31 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HSKP1"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pHSKP1-4"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Huashan Hospital"
     gene            complement(287..415)
                     /locus_tag="NIL09_RS27570"
     CDS             complement(287..415)
                     /locus_tag="NIL09_RS27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            578..802
                     /locus_tag="NIL09_RS27575"
                     /old_locus_tag="NIL09_27560"
     CDS             578..802
                     /locus_tag="NIL09_RS27575"
                     /old_locus_tag="NIL09_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            786..1052
                     /locus_tag="NIL09_RS27580"
                     /old_locus_tag="NIL09_27565"
     CDS             786..1052
                     /locus_tag="NIL09_RS27580"
                     /old_locus_tag="NIL09_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1122..1472)
                     /locus_tag="NIL09_RS27585"
                     /old_locus_tag="NIL09_27570"
     CDS             complement(1122..1472)
                     /locus_tag="NIL09_RS27585"
                     /old_locus_tag="NIL09_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1538..2083)
                     /locus_tag="NIL09_RS27590"
                     /old_locus_tag="NIL09_27575"
     CDS             complement(1538..2083)
                     /locus_tag="NIL09_RS27590"
                     /old_locus_tag="NIL09_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2109..2435)
                     /locus_tag="NIL09_RS27595"
                     /old_locus_tag="NIL09_27580"
     CDS             complement(2109..2435)
                     /locus_tag="NIL09_RS27595"
                     /old_locus_tag="NIL09_27580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2793..2951)
                     /locus_tag="NIL09_RS27705"
     CDS             complement(2793..2951)
                     /locus_tag="NIL09_RS27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3591..3767
                     /locus_tag="NIL09_RS27600"
                     /old_locus_tag="NIL09_27585"
     CDS             3591..3767
                     /locus_tag="NIL09_RS27600"
                     /old_locus_tag="NIL09_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3757..4101
                     /locus_tag="NIL09_RS27605"
                     /old_locus_tag="NIL09_27590"
     CDS             3757..4101
                     /locus_tag="NIL09_RS27605"
                     /old_locus_tag="NIL09_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4310..>4744
                     /locus_tag="NIL09_RS27610"
                     /old_locus_tag="NIL09_27595"
                     /pseudo=""
     CDS             4310..>4744
                     /locus_tag="NIL09_RS27610"
                     /old_locus_tag="NIL09_27595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4779..5288
                     /locus_tag="NIL09_RS27615"
                     /old_locus_tag="NIL09_27600"
     CDS             4779..5288
                     /locus_tag="NIL09_RS27615"
                     /old_locus_tag="NIL09_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5295..5507
                     /locus_tag="NIL09_RS27620"
                     /old_locus_tag="NIL09_27605"
     CDS             5295..5507
                     /locus_tag="NIL09_RS27620"
                     /old_locus_tag="NIL09_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3426..3505
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2843..3240)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4092..5596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.801"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cgaagctatg acggtccgtc gctgggctga aatgcaggaa aacagggttc tcatcgggca
       61 tttacgctga aaccggatgt cattcacctg gctgcatggc tatgcagcca ggtaaaattt
      121 atctgtcgcg aacggggcgg tttatcgggt ggtttgttgc cggttttacc tgactgccac
      181 cccgttcgcg gcgaacccgt ccggggcggt gcgggcaacg ggtgttatgt ttaatcagcg
      241 tgaacggcag atacaaaaaa gccccggagg gcttttaact actgacttat ttttttgaag
      301 atttgctggg tttcaatttg tcagtatttc ccggcgtttt tttattgtca cgctggcgtt
      361 tatcaggttc ccctgttgaa tcggcaattc gtttcggacc cggcttaaga cccataatcg
      421 ttatcctttc aatagttaag aggaaagccc tcaagctaga tatattgacc gcgttacgga
      481 ttatcaagag gggctaacaa gattattttt tgttctttta ggttgattgt ggtactgttg
      541 atatacatgt atagcaaatg gtgttcggga ggtttatatg cttgctatcc ggttatctga
      601 tgagattgag tcccgtctgg actcgctggc gaagcaaacc ggcagaacaa agacgtttta
      661 tgcgcgggaa gcaatactgg cgcatctgga agacctggag gattattatc tttcagcaga
      721 aactgctgca cgcgttcgcc gtggtgatga agcagtgcat tcgtctgaag acgtgaggaa
      781 gtcacttggt ctggacgatt aactattccg atcgggcgct caaatcgtta cgcaagatgg
      841 acaaacagaa cgcacgacgg attgtggatt ttatgagttt acgcattgca gttgctgccg
      901 atcctcgcca gtcagggaag ccgctcaaag gtgagctggg cgagttctgg cgctatcggg
      961 tgggagatta tcgcgttctg tgtgagatcc gagatgacga gcttgttatc cttgccgcca
     1021 cgattggaca tcgccgcgaa gtttacgact gaagcccgct atgcgggctt ttttgatgcc
     1081 gacaacaggt attatggtaa atactcgact aactacgtgt tctactgttt gtcggtttta
     1141 gttaaccagc aggttgtctt aacgataatg tagccgttat ttttaactaa taaggtcata
     1201 ttgttaacgt caggcataat aaaagacatt atggcctcag agttatcatc agaagcagct
     1261 ctctgcatta ctacttcgat tcccttttct ttgaatctat aagtatccag ttgcctatta
     1321 aacttagaaa gacctacaac catacttagt ttgttatctt taacagtaag agatgcttcc
     1381 tgagtaccat tacatcggta gtaagcggtg taaccgtcag caaaagatcg accggggaat
     1441 ataaatgcta ggaacgcaat taaccaatac atagttgaga atcttggtat tacacatttc
     1501 actatcctct ctttcatttt tcgttcacca tactttgtta tctgattttt aatttttcta
     1561 ttttataaac caagtctcta ccaaaaaggc gaaaattatc ctcaaataat aataattgtt
     1621 taagaacatc catgaaagca gctgcttcac cctcagttat gggagctact ctatataaca
     1681 tataacctga gttaagggtt tcttttatac tttcttcaat gctcattata aatattagtg
     1741 gattatcatt tttcttcccc catgggtttc taattaatcc ttcgatgtac tcaggtcctt
     1801 tgcgtcctat tgcctgcgca atatttttta tttcatctgc gtgagggctt tgttgtacag
     1861 ctttgtttat gcctgcttga gtgattttca gagactctac accgtgtatg ttgtatgaga
     1921 taaaatgtaa taaattaaga tgcaatgcaa ttaatccatt tttgtttaca tctgagtatt
     1981 tggataaaaa acgtaatgaa gtgttaactc tcccttgaag cctaacaatt gggagtgctg
     2041 ccttgtatat ttccttctcc gttaaatacc gtttaaaaaa cataattctc cgcctccgta
     2101 tagcgtgcct attttatttc gtcgtaaaac ttagctctga aatattcctt tagaaccatc
     2161 atgtcattaa taattgaatc tatctcatcc attttttctt ggtattcttt taatggtgaa
     2221 gggagcggta actcaatatt tgaaaagtga ttggcgagat agggatcaag gtttctcatc
     2281 cattcaggct tttcgccaat gcgttcttca actgaaagtg aacctgataa tctctggtta
     2341 ataagatcac ttagactcat tttggtttta ttcgaaagcc gttgtaagga ttgctttaat
     2401 cctatatcta gcatgatatt tgttctttct ctcattaccc ccccttgcta ttgcgttttt
     2461 tgttgcgcac aaagctacca ttgcgcatta agaaacgcaa taaaaaagga gggagggagt
     2521 ggtttgtaca tgatgtgcag tgcgctgagg gcatcagtgg cgggtgtttt tccgtgggat
     2581 ggatggagct ccggggataa cagaactgac gatcatttaa aggatcttct tgagatcctt
     2641 tttttctgcg cgtaatctct tgcactgtaa acgaaaaaac cgcctgggga ggcggtttga
     2701 tcgaaggtta agtcagttgg ggaactgctt aaccgggtaa ctgggtatac gagaccgcag
     2761 ataccaaaac tgttctttca gtgtagccgc agttaggcac ccacttcaag acctctcaat
     2821 atctctctcg tatatatcca gttaccaatg gctgctgcca gtggcgtttt tgcgtgtccg
     2881 tccgggttgg actcaagatg atagttaccg gaaagggcgc agcagtcggg ctgaacgggg
     2941 ggttcgtgca tacagcccag cttggagcga acgacctaaa ccgagccgag ataccagcag
     3001 cgtgagctat gagaaagcgc cacgcttccc gaaagggaaa aaggcggaac aggtatccgg
     3061 taaacggcag ggtcggaaca ggagagcgca cgagggagcc gccggagaga aatcaccggc
     3121 atctttaagt cctgtcgggt ttcgtcctcc tctgacttga gcgtccattt ctgtgatgct
     3181 cgtcagggga ggcggagcct atggaaaaac ggcaacgccg catccttttc tccggccctt
     3241 tctgatttga gcgcctttgc tttatcccgt tatccgtgag tcggtcacgc ccgccgcagc
     3301 cgaacggcag gagcgcagct accgagtgag cgaggaggcg tcatttcatc tgtccgtgca
     3361 tggtattgcg gacgctcctg agccacatat cacacctgcc acatgaggca ctttcatgaa
     3421 tcgtgctctc atgccaacat agtaagccag tatacacagc gtaaacgctg tgactggttc
     3481 agggctgcgc cccgaaaccc gctaacacca ctgacgcgct ggcgcttgtc caacgccgga
     3541 gcgacccgga aaacttttcc gtgccgtccg gcgtttgcga aggagggata atgcagaagc
     3601 cgaagaagaa gccgctcgtc gtaaaaaaaa gatcctggca tccggcagtg atggcaaatc
     3661 tgatgcgtca ggcacaggag gaagattacc gccgggagca gatggcagag cttgagcgtt
     3721 cagcccggtc acattttgcg gatatcggag gagaggatgg cggttaagcg tacccgtttt
     3781 ttaggtatcc gggtgaccga tggggagtac cagcagctgc tggagcgctg caatggaagg
     3841 caactggcgg tgtggatgcg ggaaacctgt ctcgatacgc gcccggcgcg gtcgttgcgg
     3901 ctgccgtcca tcgatccggt tttgctgcgc cagcttgccg ggatggggaa caacctcaac
     3961 cagatcgccc ggaaaattaa cggcggccag tggtcaggcg ctgaccgggt tcaggtggtg
     4021 gccgcgctga tggccattga tgccgaactc gagcggctgc ggcatgccgt gctggaaaag
     4081 ggggcagacg atgatcgtta agtttcatcc gcgagggcga ggcggcggtg ccgggccggt
     4141 ggattatctg ctggggaaag accgccagcg cgacggggcc atcgtcctgc agggaaagcc
     4201 ggaggaggtc cgggagctta tcgacgcctc gccctacgcc aaaaagtaca cctccggcgt
     4261 cctgtctttt gctgaacagg atttaccgcc cggccagcgt gaaaagctga tggcgagctt
     4321 cgagcgggtt ctgatgcccg gactcgataa agaccagtac agcgtgctgt gggttgaaca
     4381 ccgggacaag gggcggctgg aactgaattt tctggtaccg aacacggaac tgctgaccgg
     4441 caggcgtctc cagccgtact acgaccgggc agaccgtccg cgcatcgatg cgtggcagac
     4501 cgtggtgaac gggcgactgg ggctgcacga cccgaacgca ccggagaacc ggcgcgcgct
     4561 ggtgacgcca tcttcgttac ccaaaacgaa gcaggaagcc gcagaggcta ttacgcgggg
     4621 cttactggcc ctcgcctcgt caggggagct taaaacgcgt caggacgtca ctgaggcgct
     4681 ggaaagcgca ggttttgagg tcgtgcgcac cacaaagagc agcatcagca ttgccgaccc
     4741 ggacggaggg cgaaacatcc gacttaaggg agccatctat gaacagtctt ttaacgctgg
     4801 cgaaggactt agagcagaaa tcgaaagcgc agcagcagag taccggcgaa atgctgaaag
     4861 ccgcattcag cgagcacgag aagtctgtca gagcggaact gagcgaaagc gggaagagaa
     4921 tcagcgccgc catccgcgcc cacgagcagg gtatgagcgc ggccatgcag tcgaaccgcc
     4981 tgagcgtgat gcgtatggtc gggcggacat ggctgaccat cacgatggtg tcaggactgc
     5041 tgttcgccag cctgagcggg gtgttgtggt atcaggggag cttgatagcg tcaaatctgg
     5101 ccgaaatcga ccggcagaac gcagcgctgt cgaagctgaa cgcgaagacc tggggcgtga
     5161 cgtacctgga agacagcaac ggacgttttc tggtgctgcc gaaggggacg gccgtagaca
     5221 ggacgcagag ctggacggtc gggaacgggc ggtcgaagca gaacgcgctg agactggtga
     5281 aggagtaagt gccgatgacc gagctggaaa aacagttgct gagcgcatta gagcagctac
     5341 agcaggacta ctcgaaaagg ctggacgagt gggagagcgc cttcggggaa tggcggaaaa
     5401 tgtcagggct tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgacg cgcttgagtc
     5461 agcaggtgga acgcttgagc gggcaactgc agcgtttgag ccggtagtgc agcgtcatga
     5521 gatggccgtg gccgcagaac gggcgcacga gcaacggcag cacgaaaagg aactgacgga
     5581 agcgcgttcc cggcag
//
