LOCUS       NZ_CP100117            10061 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain HSKP1 plasmid pHSKP1-3, complete
            sequence.
ACCESSION   NZ_CP100117
VERSION     NZ_CP100117.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388305
            Assembly: GCF_025884255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:04:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,412
            Genes (coding)                    :: 5,235
            CDSs (with protein)               :: 5,235
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 71 of 177
            Pseudo Genes (incomplete)         :: 115 of 177
            Pseudo Genes (internal stop)      :: 25 of 177
            Pseudo Genes (multiple problems)  :: 31 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100117.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HSKP1"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pHSKP1-3"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Huashan Hospital"
     gene            complement(join(9265..10061,1..1168))
                     /locus_tag="NIL09_RS27505"
                     /old_locus_tag="NIL09_27495"
     CDS             complement(join(9265..10061,1..1168))
                     /locus_tag="NIL09_RS27505"
                     /old_locus_tag="NIL09_27495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2653..2802)
                     /locus_tag="NIL09_RS27510"
                     /old_locus_tag="NIL09_27500"
     CDS             complement(2653..2802)
                     /locus_tag="NIL09_RS27510"
                     /old_locus_tag="NIL09_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2887..3144)
                     /locus_tag="NIL09_RS27515"
                     /old_locus_tag="NIL09_27505"
     CDS             complement(2887..3144)
                     /locus_tag="NIL09_RS27515"
                     /old_locus_tag="NIL09_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3154..4839)
                     /locus_tag="NIL09_RS27520"
                     /old_locus_tag="NIL09_27510"
     CDS             complement(3154..4839)
                     /locus_tag="NIL09_RS27520"
                     /old_locus_tag="NIL09_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5166..5411
                     /locus_tag="NIL09_RS27525"
                     /old_locus_tag="NIL09_27515"
     CDS             5166..5411
                     /locus_tag="NIL09_RS27525"
                     /old_locus_tag="NIL09_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5685..6056
                     /gene="tolA"
                     /locus_tag="NIL09_RS27530"
                     /old_locus_tag="NIL09_27520"
     CDS             5685..6056
                     /gene="tolA"
                     /locus_tag="NIL09_RS27530"
                     /old_locus_tag="NIL09_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6053..6418
                     /locus_tag="NIL09_RS27535"
                     /old_locus_tag="NIL09_27525"
     CDS             6053..6418
                     /locus_tag="NIL09_RS27535"
                     /old_locus_tag="NIL09_27525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            6593..6871
                     /locus_tag="NIL09_RS27540"
                     /old_locus_tag="NIL09_27530"
     CDS             6593..6871
                     /locus_tag="NIL09_RS27540"
                     /old_locus_tag="NIL09_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6859..7173
                     /locus_tag="NIL09_RS27545"
                     /old_locus_tag="NIL09_27535"
     CDS             6859..7173
                     /locus_tag="NIL09_RS27545"
                     /old_locus_tag="NIL09_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7337..7765
                     /locus_tag="NIL09_RS27550"
                     /old_locus_tag="NIL09_27540"
     CDS             7337..7765
                     /locus_tag="NIL09_RS27550"
                     /old_locus_tag="NIL09_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7791..7970
                     /locus_tag="NIL09_RS27555"
                     /old_locus_tag="NIL09_27545"
     CDS             7791..7970
                     /locus_tag="NIL09_RS27555"
                     /old_locus_tag="NIL09_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7997..8527)
                     /locus_tag="NIL09_RS27560"
                     /old_locus_tag="NIL09_27550"
     CDS             complement(7997..8527)
                     /locus_tag="NIL09_RS27560"
                     /old_locus_tag="NIL09_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8534..9265)
                     /gene="mobC"
                     /locus_tag="NIL09_RS27565"
                     /old_locus_tag="NIL09_27555"
     CDS             complement(8534..9265)
                     /gene="mobC"
                     /locus_tag="NIL09_RS27565"
                     /old_locus_tag="NIL09_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9839
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1971..2550
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.603"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8538..9265)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gggcctccgg caacgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggga
       61 actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt
      121 cgccggtctc actcaggtta aacccggcaa ccagcagatt cttcagggcg tactggtcga
      181 gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt
      241 ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca
      301 ccacagcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct
      361 tgccgctccc gggcaggccg aggatctgaa tattggtctt gcgccaggta tcacgaggga
      421 ccagcaccgg ctggccctgc tcatccttac ccagaaacac cgcctgctgt ttccgggcct
      481 gctccgtgga aaaatcggcc agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc
      541 ggacgtctgt catttctccg gtccgctggc tgctgcgccg gctctcctga ttcagtttcg
      601 cgttcaccgc ctcgtcagcg gccgcaaaat gaccatcaag cagcaggaac gccagcagcc
      661 gcgccagaag gacgccggcg acaagccctc cggcacacca ggctatcccg aacaggttag
      721 ccggctgcag caggtaataa tctgcgttcg catagaggtc cgggcgcagg acggtttctc
      781 cccagctctc gataaacacg gcgacaacca gcccccccag aagggccaga ccggcagcgc
      841 cggtcagcca gtacgcgtag ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg
      901 aatccagcga ggccagcacc gaaaatggca cagtccggtt attatcgcgc ttatagcgat
      961 cggcatactc ccgccgcgcc ttatggctca gccccacaaa acgcattcgg ccggcccaga
     1021 gttcagccag accgaaaaac aggccgataa ccagaaaaaa accggccaga ctgtaatact
     1081 cccggtacag ccagctgacc cacgggtaaa tctgccgggc cagctccggg atatgccact
     1141 gccaggcaag cgaatccgta ttaaacatgc aatctctctc cttcagacga cgggtttacc
     1201 ggcgtgatgc gcagcccccg ctgcacaagc cgcggacatg ccccaaggca tggcggcggc
     1261 ggtcacgtcc gtggtgattg cgttcagctt tcaccgcgga ggccagcctc tttccgcccc
     1321 atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa
     1381 ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc cgtcagagtt cgccatagcg
     1441 agcggctgac cacccgcaaa atcaggagca gctttgctgc ttcgtcaggt gcgtccagca
     1501 ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg ggccgggtga atttctggcg
     1561 tggccggcgt ccagccggac agagccagaa agaggggaaa agaccgtgcg tcggtggcgt
     1621 ccagccagcg taagcaaggt cttttcccct gaagtcagcc acgccccagc ggggatgact
     1681 tgcatttctc cgcaaggaga aaaaagcaac agagataggt taacgtaaaa tgttcagcga
     1741 aaaaccgaca tggttcacct atcctgataa ttgatcgtca gggaatagaa agacttaatc
     1801 aggggacaat atcccacatc agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg
     1861 cgcctcctcg ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
     1921 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
     1981 cggcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
     2041 aaacagacgc tttttcggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
     2101 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggtaggt cgctccctct
     2161 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccgtt ctcccttgcg
     2221 ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc
     2281 gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg
     2341 gtaactttca tcttgagtcc aacccgggaa agacacgcaa aaacgccact ggcagcagcc
     2401 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
     2461 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
     2521 agttacccgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg
     2581 tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc
     2641 ctttgatctt ttctactgaa ccgcgatccc cgtcagttca gaggaggacg atggtgccac
     2701 cgttccgccc tgaacatccc ggatataatt tgcctgacat gcggccagaa gaaagccagc
     2761 aacaatcaat ataaaaagaa aaatagtctt tacctttttc atatactttc cctgtattta
     2821 aagactccgg cattcatccc tgaacaccgg aagtcagtat ttcaaatcat ccttgataat
     2881 tttttgttac cagttaccgt cccgataatc aaaagctata taacaattat ccacatcagg
     2941 ttttatttga tttttaaaaa atggctgtag taaaggaacc cattctttct tcaaatcaaa
     3001 ctcaccatta ttaatattat cctttaaagg cattcccaag ctttcaatga cagaaccatc
     3061 gtcaccaaag tctttagaat actcttcccc tttaaactct tcagttttct tatcaaacca
     3121 tgtcaaacgt aatttaagcc ccatatttac ctctcaaaga tattttttaa tgttccgttt
     3181 tggatctggc cctttaacct gcttacccgt tttgggatca aatgcgccta ggtgttcgcc
     3241 atcactggca cgataccctt caagctcacc gtgctgggaa tcccattcat aaattttacg
     3301 ttttttatcg ccataccatc gggcccgctt accaccacca ccttgtttag gtgttttagg
     3361 atcacctcgc tttaactctc caagtccctt tatgtcttct gttttaggta ctggatgata
     3421 atcatggccg tagtctttag tccccttgcg aggcttgttc ttttcctcat ttaacttatt
     3481 ttctgcatcc ttcgcttttt tctccttttg tttccggctc tccatcgccc cggtaagtgc
     3541 agccttatct ctggataagg attcattcat tgcgtttaaa gatgcctgag ccgcttttaa
     3601 tttctcattc tgattctgaa tatccttatc aagatagcga acctgatcct gccacccgga
     3661 gccatattca tgaggcgggt tataagtcac aaaatcatct ttatttgcct ctgcctcttt
     3721 taccttttta tcggcttcct gaacagcagc ccttgcaccg gggagcgcct tctctgtcga
     3781 tgcaatgcgg ctgtttaagg tcgcaatatt tttatcttcg gcatccagct cagctttcgc
     3841 tttatcatag tctcttttca gcccctcttc cggatgagcg gcgtcccatg cctgctggcg
     3901 acgcttttct tcctcctgac gctgttttac ctgtgccggg gtaagaacat ctgtcaccga
     3961 cacataaacc ggcttctgtc cggtctcttt cgggaaacga ataacggcct cacgggagtt
     4021 accaccggct gtaaaaccag ccggacgtga atcacctttt tcagcaacaa tgccttttgg
     4081 cggggctttc gctgtcggaa cacctttcgg tacgctcacc tgcagagacg ggagacccgg
     4141 aatcgacaca ctgaataccc ccggacgttt tgtcggtttc gcatccacca cagggacact
     4201 cattggccga cctgcgacaa ccgcaatatg ctgccgctca tccttcacca catccacaac
     4261 ccgttgtctg acacgaaccg tcgcctggtc cagtggtaaa gtacttgccg gagtctccgt
     4321 caccgcatcg gccggtaatg acgtcataat ttttgacatc attttcggat catcttttgc
     4381 aatctcagaa ggcagcacac cgtagatcgc aatcccccac agaccaaact taaacggccc
     4441 cttcagggca gccagcacat cagcaacggc ggccgacaac gcatcgccgg aaactgataa
     4501 agccagtcct tcagcccccg gagtagccag ggcaggcaga ccgaaggcca tcgcagtggc
     4561 cgaagactgt ccgccaccag agctaccgct atgattgccg ctgttaccac cattaccacc
     4621 gggtttactc ccgccgttac caaactcccc cggattatag ttatgaatat ctccattcgg
     4681 cgtatgcgtc gtaccccagc ccggcccgct gtttggatcc gtaccaccac ctgatgaagg
     4741 gccaccttta ccagacgtac cgttcacatt cccactggcc tgaccaccat tatgtcccag
     4801 acctgaatta cccggacctc gaccgtctcc accactcata ccgttttcct ctttgacatt
     4861 taaaacaata aattaaagcc aacatactgt acatatacac agtataatta ttaccttact
     4921 cctcccgttt tcaggtgtca atatatgaga ttagttctca tcttcaggca caaaaaagcg
     4981 cccggagcca cctgtaagca gatcgcgtct tttcccgaaa atttctcatg gcaacaaatt
     5041 ttattattta agacattcat cacacttaca tttcagatgg tatagacaca tctgaaagct
     5101 aactggcatg aggagaggac ctgcggttaa actgttcggg aaacctgaga cgcccggagg
     5161 ccactatgac gcacctgaaa ctgactcccg ctgaagaaaa gcgcctggcg gacgcctttg
     5221 tcgctcaggc actggccatg ggcctgaaag ggaacaaaac gctgccaaag gcggtaaaac
     5281 aacaggtgcg ggaaagggtg cttcgtgcca ttcaggacga acgtgaagcc aggcgggcgc
     5341 aggaaatgcg tcgggagaaa tacgaggcta cggaaaccac cttcacctgg cagccacagc
     5401 gccgccggta aggggtattt accataacgt cgcattgccc gcaccgctca gacggtctga
     5461 gggcgtttcc ggcggctacg ggataaaaca ggcaacaaac cacccggaaa accgccggaa
     5521 acgcgcccgg taaattgtaa cccgctgcat gcctatgcac gcaactgcat ggcgggccgt
     5581 ttggtgtaaa aacgatacga taagccgtgt ttatgtaaat gaacgtggcc gtaaagagtg
     5641 acaaatatac atgtaacatt ttttactgac aggtcatcaa cccgatgcgt ttaataccct
     5701 ttatcctgct ggcattgtgc agccccttac tcctgacggc ctgtaagcat tccaccaccc
     5761 agccgccgaa aatttatact ctggataagt catctaaaat cagtagctac gctgcggcca
     5821 tcaagaccgc tgttgaaatg cagatgtttg aaccagaggc ctatgcggga aaaatgtgca
     5881 ccgtgcggat atccatacaa cgtgatggca ctctgaacag cgccattgct gaaggaggag
     5941 atccgaaact ctgtaaagca gcaatatcag ccgttaccca cgccaaaatt ccgccggcac
     6001 cggatgaaaa tacctggcaa atattcaaaa acgcccctct ggatttcaga ccatgaaagc
     6061 ttttactctg acagtgctgg cggccatgtt cctcactccg gtacatgcga taccactaaa
     6121 acccgtaacc acactgcagc caacctaccc ggtacaggcc gcagcaaaac gcatcgaagg
     6181 tcaggtgacc gccacgtttg atattctgga cgatggacga gtggacaacg tgtcgcttac
     6241 cggctgtcac cttttttttc gggaaacgct gacggcaatg aggcactggc tatatcccgc
     6301 agggcacccg gcaatgcgac aggttataaa ttttgaattc agtctgaaaa atgacggctt
     6361 acaggatccg cgaccagtca ccacccaaaa gctcaatgat gatggttgcc gccagtaaca
     6421 ggtcctttaa cccgcaggct gcaatagtcc tggatgatga gtgagcctgt agcagccctg
     6481 attagtggaa gcgaagctga aaaatgggca gcaaaagccg tatttctggc aaatagcgcc
     6541 gccacggatt aaagtagtca tgtgtaatac atatgactac aaaggggctt ttatgtcatc
     6601 agcaacatcc atcaggctcg atgaagaact taaggaccgg ctgaaaacgc tggccgatga
     6661 ccggcaccgc tcagcgcacg cgctaatgct ggaggcaatc accgaataca tcgaccggga
     6721 agagaagcgc agccagtacc tgcgggacgg acaggccgca tggcagcact atcaggaaac
     6781 gggtctgcac cttactgcgg aagaagcgga agcctggata agtacctggg gaactgagaa
     6841 cgagcaggat gctccgccat gccacaggta acgatttccg cgctggcaca gcgcgattta
     6901 caacgccttc aggactttct caaaaccaaa aatcggctgg cagccagaaa aggaggtgag
     6961 gtgatcgtcc gggctatcca gcaactgaaa acattgccag acattggccg cccggtgccg
     7021 tttctgccgc tggaatataa ggaactggtg atcgggtttg gcgacagtgg ctatgtgatg
     7081 ctctaccgcc acgaccggga aatggaccag attgtgatcg tcaccgtcag gcatcagaaa
     7141 gaatccgggt atccgggggc agacagcctc taataaccga tgacgggggc gcaattcatt
     7201 gcgtggccgt cgccggtttg acaagcgcag cgcgtcagaa aaccacatcc tgaacggaaa
     7261 aacgggtaaa attcagcgat acatttaccg tgagaaatta cacacctttc acacccgata
     7321 aaatcagggg tatacagtgg cgaaaacatt gctcaacctt tcacaggcag cacaagccgc
     7381 gggcattacc agaagaacgc tatacaatca tgtaaaacag ggaaaggtta ccgtgtcacg
     7441 tgacgggaaa aacaaccctg tcgtggatgt gtcagaactg atccgtgtat acggaaatgt
     7501 gaatatccct gagaaacaaa ttcccggcat ttcacaccgt gaaaatacac aaaaaaattt
     7561 cccacaggaa cagcttctgg caatgcaaaa ggaactggcc gatctccggc aggctgtcac
     7621 cctgatgctg gaagataaaa catcccggga agaagaacgc cgccagcacg atgatgagcg
     7681 ccggaaactc caggccgaag tggacagatt gacaacagaa ctgacacaga agaaaaaacg
     7741 cttctggtcc ggctggttta gctgagaact taccgaaggg aatggagaac atgacgaagc
     7801 aggaaaaaac tgccctcaat atggctaaat tcattaagga tcagtcgctg ctgttgctgg
     7861 aaaagctgaa cgagctggac atggataccc cggccgacat gtgcgaacgg cttcacgagg
     7921 atgcggaaac actctggcag gtgatgcggg ataagctcgg ggaagaataa cagcaccggc
     7981 catgccggtg ctggcgttat tttttgccga acggccacca ccggcgggcg ggtttgtcct
     8041 ccggcagttc aggcaccggc acgttaaaat agcggctgtt atattcccgc atcagctccg
     8101 ctttcagctt ctcgttcacc gcgctttccc cgtgccgctc gctgaccacc tcaatcatcg
     8161 cgttaatctg ctccggtgag cacttcccgg catcaaccac ctgcacaccg ttctcgatgg
     8221 cgaaccgctc cacctttcga tagaggtaat cacacagctt cattcctgtg ggtttgtccc
     8281 gttcccattg cggattttca ccggtgaaaa agtacgtggc tttttcgtcg ctgttgagcc
     8341 gctcgtcata catcccgatg gatttcatca tcgccagatg ctgggcccgg tcgacaaaac
     8401 agaaaacata cgccccggtc tgccaggcga gcaccaccat gtcttccaga tgctcgctgg
     8461 ttggcataat gcagcgctcg ccgtctttgt cttccacgtc ctcaataaat accatgtaca
     8521 gcgccataat ccctcactcc gttgtcataa atttaaagca cgcgaacagc ctctcccggt
     8581 ccagctccac cgttttgcgg gcctcgccgg tctgtggatt cacgatttca tccaccgcca
     8641 gaatcgcccg ctgtacccgg acaagcgtcg gccggtcagg gcagatgtac cagatttcat
     8701 cgatgccgtt cgcccggcgg ttgaacagat acccggacac cacttcctga taccgcctgc
     8761 ggctcttgac ggtgcgctct acctcaaatg ccaccgttct gccgtccagc tccgccagcg
     8821 cgtccggcac tttcatccct ttcagggcca tacggtgcag gtaccgccag ccggtggccc
     8881 cccggccctc catggagagg cgcagggact gcacggccag ccggtgcggc agctgcgccg
     8941 ccgacaccct tgacggctga aacggaataa cgtccgtcag cggctcgtcc tcgtcgaacg
     9001 agaacgccac cccgtgcggc gtcagccccc acacgctgac ctgacggccg tcaaccgggt
     9061 atttcgcccg gaccagcagc cctttcgcca ccagactgtc caggaagcgg taaaccggct
     9121 gtaccgccgc aatccccagc cgtcgcgcaa ccagctccgc cgtggaacac agctcttccc
     9181 gcagccagcg cagggtcagc cgtgttttct ccgcgttccg cgcctgccgc ttcgcgtggc
     9241 tgacgttgta ctgctccagt gtcatcagta aagccccgca aaatcatcat ccgcttttgg
     9301 tccgccgccg tcggccggag gcaggtactc cgccagcggg tccgttttcg ccagcaccgg
     9361 gaaggttttc agcgaaattt cccggcgctc aacccgcagc gggctgctga acgccagccg
     9421 cgccacgccc agcccggtca tcaccgcgaa cccgtcaggc aggtgttgca gggtgttcac
     9481 gtcaaacagc ggccgcgcct ccttctggat gaagctgtcg ccgctgatgt gctccacgtt
     9541 ccccgcctcc cggctggccc ggcggcgctc cacgtccacc cggatctccc cggtctggcc
     9601 cgccgcccac tgcgcggact cctggctggc cgcgcgatag aaccagcgga tcggcgtgtt
     9661 atccagtacc gtggttttca caaaatcagc gggcagatcc tgcccgcact gaccgaagtc
     9721 gcccagcgac tggtgcgcga gcagcaggtt gcagtcccgg ctgcgcagcg tccccagcgc
     9781 gttcagcacg tacttcgaca gcagatattt aatctcatcg agcatgatgc tcgcgtgcgg
     9841 ccacctccgg aactcatccc gcgcaatgat gatttgcgcg cagcgggcaa acagcatctt
     9901 ctgcacccgg atcaccgcct catcgtccat cgagccgatg acgtacaggc agccgccgcc
     9961 gttgattatc cccgccacat cgataccgct gtccgtctgc aggacgctca ggtcggccac
    10021 gttctccagc ttggtgatca gccccttcac gtcctttttc a
//
