LOCUS       NZ_CP100093             2953 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain HSKP8 plasmid pHSKP8-6, complete
            sequence.
ACCESSION   NZ_CP100093
VERSION     NZ_CP100093.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388311
            Assembly: GCF_025884135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:03:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,694
            CDSs (total)                      :: 5,570
            Genes (coding)                    :: 5,292
            CDSs (with protein)               :: 5,292
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 184 of 278
            Pseudo Genes (incomplete)         :: 117 of 278
            Pseudo Genes (internal stop)      :: 25 of 278
            Pseudo Genes (multiple problems)  :: 43 of 278
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100093.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HSKP8"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pHSKP8-6"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Huashan Hospital"
     gene            683..1657
                     /gene="mobV"
                     /locus_tag="NIL17_RS28425"
                     /old_locus_tag="NIL17_28400"
     CDS             683..1657
                     /gene="mobV"
                     /locus_tag="NIL17_RS28425"
                     /old_locus_tag="NIL17_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            complement(1684..2034)
                     /locus_tag="NIL17_RS28430"
                     /old_locus_tag="NIL17_28405"
     CDS             complement(1684..2034)
                     /locus_tag="NIL17_RS28430"
                     /old_locus_tag="NIL17_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(2031..2582)
                     /locus_tag="NIL17_RS28435"
                     /old_locus_tag="NIL17_28410"
     CDS             complement(2031..2582)
                     /locus_tag="NIL17_RS28435"
                     /old_locus_tag="NIL17_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
ORIGIN
        1 cgctgccccc cgccaccccc cgcgttcgcc acgtggcagg ggtgcagggg ctacgggcct
       61 gcggcccttc actcctgcac ccctaccggg gagcgtgggg tcgttccagg cgcgtcaagg
      121 gccgctgcgc tctccggggg tggcccgtgt cctcgccctg cgggctgcgg gccgcgccag
      181 ccaccccctg cgcccttgac ccgccttcca ctccggcgct gtcggaaatt tcctacccgg
      241 ccatgaggcg atttccgaca cgccagcggg ccactggctg acgccttcag cggagggcag
      301 ggtcaagggc gcgcccgcag gccgcagccc ggagcgaagc gcagggaccg ggcgaggacg
      361 cgggcgaagc cgaacccttg acgctgaccg cagcgcccca gcctgtgcga gtgggggcag
      421 ggacgaaggg cgaagcccgc cacccctgcc ccccgagcct cccggcggcg agggtgggga
      481 gtccagaggg gcaaagcccc tttgggcaag gccgaaggcc gccgcaggag caagcccgaa
      541 gggcgctgca accgccgcag gcgggcacga ctttgcttcg caaagtctag tgagtacact
      601 atcatctgat agtggcccgc cggaggcacc gccttgcgct gccccgactg gccggattcg
      661 gacaccaagg ggaggggcag gcatggcggc ctacgcgatc atgcgatgca agaagctggc
      721 gacgatgggc agcgtggcgg cggcgttgca gcactgctac cgcgagcgcg aaacgcccaa
      781 cgctgacgcc gaacgcacgc cggacaacga gcaccgggcg gccacctcaa ccgatgcggc
      841 gatggggcgg ctgcgcgagt tgctgccgga gaagcggcgc aaggacgcgg tgctggcggt
      901 cgaatacgtg atgacggcca gcccggaatg gtgggccaag gccacgcccc agcagcaggc
      961 cgcgttcttc gaccaggcgc agggctggct ggcggccaaa tacggcgcgg atcgcatcgt
     1021 gacggccagc atccaccgcg acgaggccac cccgcacctg tcggccttcg tcgtgccgct
     1081 gacgcaggac gggcggctgt cggccaagga gttcatcggt ggccgcgaca agatgcgggc
     1141 cgaccagacc agctttgccg aggcggtgag ggatctcggg ctggaacgcg gcatcgaggg
     1201 cagccgggcc acccatcagc gcgtccagag ctactacggc gccatcgagc gccagcccgg
     1261 gcacgccacc atcacgccgc aggccatcga gccgcgggtg ctacgcaagg ggattttctc
     1321 gaaggacgtg gagacgccgg aggccgtggc cgcccgcgtg accgctgccg tcagggaagg
     1381 gtatggcccg accgtcgcag cggccgcagg agcgcgtcag gagcgcgaga aggcccggca
     1441 ggcacaggag acagcccgga gcctgcgtga ccgcctgaag cccgttctgg acgctctggg
     1501 gccgcttaat cgcgatatgc aggccaaggc ggcccagatc atcaaggccg tgggcgagaa
     1561 gctgctcgcc gaacagcgcg aggcgacacg ccagcggcag gcccaacggc ttcgcagtcg
     1621 cggccgggat cgcgacgacg gcgggctgtc tcgttgaccc tacaggtggc cgacggaagg
     1681 ggtttatggc ttgcagctac tgaggacggt ttgctccttc ccgccatcgt gggtcgaaag
     1741 gttcatgtcg ttgcccttac cccacagttc gtaggtgtag tttttgctag cggagatgta
     1801 gcgcactcct gatgcgctct tggcgcgttt cagtgggaca agctcatcca tttgcagcat
     1861 cacggcatag tctccgacgt acacgacatc caacgtcttt ccgccttcgc aagcgtactt
     1921 ggtctggatc atctcatccg ctgctgaagc aagattcgga gcgattgccg ccatcatggc
     1981 agcaagagcg aacgtttttt tcataaagat tttctccttc gtttcgttgc tcattgggga
     2041 gtgctttcaa acatgtcaat ggtgttcggg tcgcggtcgt cgttctgctt ctcgccgctg
     2101 aacatcgaca tgatccggcg gcggatatcg ccatccttgt tgaccaaggt tttggtcacg
     2161 aaagctgcgt aatccttggc gttgtcgtag cccttccaga cctcgtgagg atccagcgca
     2221 taggcgcaaa ccttgccagc aacagggatg cggacaagcg cgcgattttc ttccaggtaa
     2281 gacaaccaac ggctgattgt ccgcctgttg acgttcatct tgtctgccaa aaactgctga
     2341 tcgcagacca ccgcgccgca ggttgggtcg atattttcag caaagaatga atacaggccg
     2401 aaggccccag cattgccttt agcaagctca atgatgcgct tccaacccaa gggatacacc
     2461 tgcgtgaacc ccttgttctt ccgcgcctct tcgcgctcct gagcctcttt ctcgtccagc
     2521 atggccagct ctcgctggcg gatacgacgc tgggcgtcgg tcgccaccgt gatctgctgc
     2581 atggggttcc tccaaagtcg ggaaatttgt ccgctcattg cgccatttcg agtcgcaatg
     2641 atacgacaaa ttatgtctca cgtcgagaca gtttgcgtct caaccttgct acctgttgag
     2701 acatccatgc atgtctcaac gttaggacac cctatgtgtc tcaatgcatt tctacaaatc
     2761 cttataaatc aataacttag catttccccc tttctcctct aaggggctgc cgccccgccg
     2821 gcggccgcta cgcggccttg gcaccccgct gacggcccct gcggggcctc tggaaaaggc
     2881 ccacccggcc accggccggg tgacgttgtt tggggggcct ccggcggccg ctgcgcagcc
     2941 ttggggcggt ggg
//
