LOCUS       NZ_CP100092             5596 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain HSKP8 plasmid pHSKP8-5, complete
            sequence.
ACCESSION   NZ_CP100092
VERSION     NZ_CP100092.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388311
            Assembly: GCF_025884135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:03:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,694
            CDSs (total)                      :: 5,570
            Genes (coding)                    :: 5,292
            CDSs (with protein)               :: 5,292
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 184 of 278
            Pseudo Genes (incomplete)         :: 117 of 278
            Pseudo Genes (internal stop)      :: 25 of 278
            Pseudo Genes (multiple problems)  :: 43 of 278
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100092.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HSKP8"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pHSKP8-5"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Huashan Hospital"
     gene            complement(join(5592..5596,1..346))
                     /locus_tag="NIL17_RS28370"
                     /old_locus_tag="NIL17_28350"
     CDS             complement(join(5592..5596,1..346))
                     /locus_tag="NIL17_RS28370"
                     /old_locus_tag="NIL17_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(412..957)
                     /locus_tag="NIL17_RS28375"
                     /old_locus_tag="NIL17_28355"
     CDS             complement(412..957)
                     /locus_tag="NIL17_RS28375"
                     /old_locus_tag="NIL17_28355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(983..1309)
                     /locus_tag="NIL17_RS28380"
                     /old_locus_tag="NIL17_28360"
     CDS             complement(983..1309)
                     /locus_tag="NIL17_RS28380"
                     /old_locus_tag="NIL17_28360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1667..1825)
                     /locus_tag="NIL17_RS28495"
     CDS             complement(1667..1825)
                     /locus_tag="NIL17_RS28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2465..2641
                     /locus_tag="NIL17_RS28385"
                     /old_locus_tag="NIL17_28365"
     CDS             2465..2641
                     /locus_tag="NIL17_RS28385"
                     /old_locus_tag="NIL17_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2631..2975
                     /locus_tag="NIL17_RS28390"
                     /old_locus_tag="NIL17_28370"
     CDS             2631..2975
                     /locus_tag="NIL17_RS28390"
                     /old_locus_tag="NIL17_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3184..>3618
                     /locus_tag="NIL17_RS28395"
                     /old_locus_tag="NIL17_28375"
                     /pseudo=""
     CDS             3184..>3618
                     /locus_tag="NIL17_RS28395"
                     /old_locus_tag="NIL17_28375"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3653..4162
                     /locus_tag="NIL17_RS28400"
                     /old_locus_tag="NIL17_28380"
     CDS             3653..4162
                     /locus_tag="NIL17_RS28400"
                     /old_locus_tag="NIL17_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4169..4381
                     /locus_tag="NIL17_RS28405"
                     /old_locus_tag="NIL17_28385"
     CDS             4169..4381
                     /locus_tag="NIL17_RS28405"
                     /old_locus_tag="NIL17_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4757..4885)
                     /locus_tag="NIL17_RS28410"
     CDS             complement(4757..4885)
                     /locus_tag="NIL17_RS28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            5048..5272
                     /locus_tag="NIL17_RS28415"
                     /old_locus_tag="NIL17_28390"
     CDS             5048..5272
                     /locus_tag="NIL17_RS28415"
                     /old_locus_tag="NIL17_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            5256..5522
                     /locus_tag="NIL17_RS28420"
                     /old_locus_tag="NIL17_28395"
     CDS             5256..5522
                     /locus_tag="NIL17_RS28420"
                     /old_locus_tag="NIL17_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          2300..2379
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1717..2114)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2966..4497
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttgtcggt tttagttaac cagcaggttg tcttaacgat aatgtagccg ttatttttaa
       61 ctaataaggt catattgtta acgtcaggca taataaaaga cattatggcc tcagagttat
      121 catcagaagc agctctctgc attactactt cgattccctt ttctttgaat ctataagtat
      181 ccagttgcct attaaactta gaaagaccta caaccatact tagtttgtta tctttaacag
      241 taagagatgc ttcctgagta ccattacatc ggtagtaagc ggtgtaaccg tcagcaaaag
      301 atcgaccggg gaatataaat gctaggaacg caattaacca atacatagtt gagaatcttg
      361 gtattacaca tttcactatc ctctctttca tttttcgttc accatacttt gttatctgat
      421 ttttaatttt tctattttat aaaccaagtc tctaccaaaa aggcgaaaat tatcctcaaa
      481 taataataat tgtttaagaa catccatgaa agcagctgct tcaccctcag ttatgggagc
      541 tactctatat aacatataac ctgagttaag ggtttctttt atactttctt caatgctcat
      601 tataaatatt agtggattat catttttctt cccccatggg tttctaatta atccttcgat
      661 gtactcaggt cctttgcgtc ctattgcctg cgcaatattt tttatttcat ctgcgtgagg
      721 gctttgttgt acagctttgt ttatgcctgc ttgagtgatt ttcagagact ctacaccgtg
      781 tatgttgtat gagataaaat gtaataaatt aagatgcaat gcaattaatc catttttgtt
      841 tacatctgag tatttggata aaaaacgtaa tgaagtgtta actctccctt gaagcctaac
      901 aattgggagt gctgccttgt atatttcctt ctccgttaaa taccgtttaa aaaacataat
      961 tctccgcctc cgtatagcgt gcctatttta tttcgtcgta aaacttagct ctgaaatatt
     1021 cctttagaac catcatgtca ttaataattg aatctatctc atccattttt tcttggtatt
     1081 cttttaatgg tgaagggagc ggtaactcaa tatttgaaaa gtgattggcg agatagggat
     1141 caaggtttct catccattca ggcttttcgc caatgcgttc ttcaactgaa agtgaacctg
     1201 ataatctctg gttaataaga tcacttagac tcattttggt tttattcgaa agccgttgta
     1261 aggattgctt taatcctata tctagcatga tatttgttct ttctctcatt accccccctt
     1321 gctattgcgt tttttgttgc gcacaaagct accattgcgc attaagaaac gcaataaaaa
     1381 aggagggagg gagtggtttg tacatgatgt gcagtgcgct gagggcatca gtggcgggtg
     1441 tttttccgtg ggatggatgg agctccgggg ataacagaac tgacgatcat ttaaaggatc
     1501 ttcttgagat cctttttttc tgcgcgtaat ctcttgcact gtaaacgaaa aaaccgcctg
     1561 gggaggcggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgg gtaactgggt
     1621 atacgagacc gcagatacca aaactgttct ttcagtgtag ccgcagttag gcacccactt
     1681 caagacctct caatatctct ctcgtatata tccagttacc aatggctgct gccagtggcg
     1741 tttttgcgtg tccgtccggg ttggactcaa gatgatagtt accggaaagg gcgcagcagt
     1801 cgggctgaac ggggggttcg tgcatacagc ccagcttgga gcgaacgacc taaaccgagc
     1861 cgagatacca gcagcgtgag ctatgagaaa gcgccacgct tcccgaaagg gaaaaaggcg
     1921 gaacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcacgaggg agccgccgga
     1981 gagaaatcac cggcatcttt aagtcctgtc gggtttcgtc ctcctctgac ttgagcgtcc
     2041 atttctgtga tgctcgtcag gggaggcgga gcctatggaa aaacggcaac gccgcatcct
     2101 tttctccggc cctttctgat ttgagcgcct ttgctttatc ccgttatccg tgagtcggtc
     2161 acgcccgccg cagccgaacg gcaggagcgc agctaccgag tgagcgagga ggcgtcattt
     2221 catctgtccg tgcatggtat tgcggacgct cctgagccac atatcacacc tgccacatga
     2281 ggcactttca tgaatcgtgc tctcatgcca acatagtaag ccagtataca cagcgtaaac
     2341 gctgtgactg gttcagggct gcgccccgaa acccgctaac accactgacg cgctggcgct
     2401 tgtccaacgc cggagcgacc cggaaaactt ttccgtgccg tccggcgttt gcgaaggagg
     2461 gataatgcag aagccgaaga agaagccgct cgtcgtaaaa aaaagatcct ggcatccggc
     2521 agtgatggca aatctgatgc gtcaggcaca ggaggaagat taccgccggg agcagatggc
     2581 agagcttgag cgttcagccc ggtcacattt tgcggatatc ggaggagagg atggcggtta
     2641 agcgtacccg ttttttaggt atccgggtga ccgatgggga gtaccagcag ctgctggagc
     2701 gctgcaatgg aaggcaactg gcggtgtgga tgcgggaaac ctgtctcgat acgcgcccgg
     2761 cgcggtcgtt gcggctgccg tccatcgatc cggttttgct gcgccagctt gccgggatgg
     2821 ggaacaacct caaccagatc gcccggaaaa ttaacggcgg ccagtggtca ggcgctgacc
     2881 gggttcaggt ggtggccgcg ctgatggcca ttgatgccga actcgagcgg ctgcggcatg
     2941 ccgtgctgga aaagggggca gacgatgatc gttaagtttc atccgcgagg gcgaggcggc
     3001 ggtgccgggc cggtggatta tctgctgggg aaagaccgcc agcgcgacgg ggccatcgtc
     3061 ctgcagggaa agccggagga ggtccgggag cttatcgacg cctcgcccta cgccaaaaag
     3121 tacacctccg gcgtcctgtc ttttgctgaa caggatttac cgcccggcca gcgtgaaaag
     3181 ctgatggcga gcttcgagcg ggttctgatg cccggactcg ataaagacca gtacagcgtg
     3241 ctgtgggttg aacaccggga caaggggcgg ctggaactga attttctggt accgaacacg
     3301 gaactgctga ccggcaggcg tctccagccg tactacgacc gggcagaccg tccgcgcatc
     3361 gatgcgtggc agaccgtggt gaacgggcga ctggggctgc acgacccgaa cgcaccggag
     3421 aaccggcgcg cgctggtgac gccatcttcg ttacccaaaa cgaagcagga agccgcagag
     3481 gctattacgc ggggcttact ggccctcgcc tcgtcagggg agcttaaaac gcgtcaggac
     3541 gtcactgagg cgctggaaag cgcaggtttt gaggtcgtgc gcaccacaaa gagcagcatc
     3601 agcattgccg acccggacgg agggcgaaac atccgactta agggagccat ctatgaacag
     3661 tcttttaacg ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc agagtaccgg
     3721 cgaaatgctg aaagccgcat tcagcgagca cgagaagtct gtcagagcgg aactgagcga
     3781 aagcgggaag agaatcagcg ccgccatccg cgcccacgag cagggtatga gcgcggccat
     3841 gcagtcgaac cgcctgagcg tgatgcgtat ggtcgggcgg acatggctga ccatcacgat
     3901 ggtgtcagga ctgctgttcg ccagcctgag cggggtgttg tggtatcagg ggagcttgat
     3961 agcgtcaaat ctggccgaaa tcgaccggca gaacgcagcg ctgtcgaagc tgaacgcgaa
     4021 gacctggggc gtgacgtacc tggaagacag caacggacgt tttctggtgc tgccgaaggg
     4081 gacggccgta gacaggacgc agagctggac ggtcgggaac gggcggtcga agcagaacgc
     4141 gctgagactg gtgaaggagt aagtgccgat gaccgagctg gaaaaacagt tgctgagcgc
     4201 attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga gcgccttcgg
     4261 ggaatggcgg aaaatgtcag ggcttatgca acgggagaac gcggcgctga gcgagcgcgt
     4321 gacgcgcttg agtcagcagg tggaacgctt gagcgggcaa ctgcagcgtt tgagccggta
     4381 gtgcagcgtc atgagatggc cgtggccgca gaacgggcgc acgagcaacg gcagcacgaa
     4441 aaggaactga cggaagcgcg ttcccggcag cgaagctatg acggtccgtc gctgggctga
     4501 aatgcaggaa aacagggttc tcatcgggca tttacgctga aaccggatgt cattcacctg
     4561 gctgcatggc tatgcagcca ggtaaaattt atctgtcgcg aacggggcgg tttatcgggt
     4621 ggtttgttgc cggttttacc tgactgccac cccgttcgcg gcgaacccgt ccggggcggt
     4681 gcgggcaacg ggtgttatgt ttaatcagcg tgaacggcag atacaaaaaa gccccggagg
     4741 gcttttaact actgacttat ttttttgaag atttgctggg tttcaatttg tcagtatttc
     4801 ccggcgtttt tttattgtca cgctggcgtt tatcaggttc ccctgttgaa tcggcaattc
     4861 gtttcggacc cggcttaaga cccataatcg ttatcctttc aatagttaag aggaaagccc
     4921 tcaagctaga tatattgacc gcgttacgga ttatcaagag gggctaacaa gattattttt
     4981 tgttctttta ggttgattgt ggtactgttg atatacatgt atagcaaatg gtgttcggga
     5041 ggtttatatg cttgctatcc ggttatctga tgagattgag tcccgtctgg actcgctggc
     5101 gaagcaaacc ggcagaacaa agacgtttta tgcgcgggaa gcaatactgg cgcatctgga
     5161 agacctggag gattattatc tttcagcaga aactgctgca cgcgttcgcc gtggtgatga
     5221 agcagtgcat tcgtctgaag acgtgaggaa gtcacttggt ctggacgatt aactattccg
     5281 atcgggcgct caaatcgtta cgcaagatgg acaaacagaa cgcacgacgg attgtggatt
     5341 ttatgagttt acgcattgca gttgctgccg atcctcgcca gtcagggaag ccgctcaaag
     5401 gtgagctggg cgagttctgg cgctatcggg tgggagatta tcgcgttctg tgtgagatcc
     5461 gagatgacga gcttgttatc cttgccgcca cgattggaca tcgccgcgaa gtttacgact
     5521 gaagcccgct atgcgggctt ttttgatgcc gacaacaggt attatggtaa atactcgact
     5581 aactacgtgt tctact
//
