LOCUS       NZ_CP100091            10060 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain HSKP8 plasmid pHSKP8-4, complete
            sequence.
ACCESSION   NZ_CP100091
VERSION     NZ_CP100091.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388311
            Assembly: GCF_025884135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:03:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,694
            CDSs (total)                      :: 5,570
            Genes (coding)                    :: 5,292
            CDSs (with protein)               :: 5,292
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 184 of 278
            Pseudo Genes (incomplete)         :: 117 of 278
            Pseudo Genes (internal stop)      :: 25 of 278
            Pseudo Genes (multiple problems)  :: 43 of 278
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100091.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HSKP8"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pHSKP8-4"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Huashan Hospital"
     gene            join(8675..10060,1..579)
                     /locus_tag="NIL17_RS28305"
                     /old_locus_tag="NIL17_28285"
     CDS             join(8675..10060,1..579)
                     /locus_tag="NIL17_RS28305"
                     /old_locus_tag="NIL17_28285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            579..1310
                     /gene="mobC"
                     /locus_tag="NIL17_RS28310"
                     /old_locus_tag="NIL17_28290"
     CDS             579..1310
                     /gene="mobC"
                     /locus_tag="NIL17_RS28310"
                     /old_locus_tag="NIL17_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1317..1847
                     /locus_tag="NIL17_RS28315"
                     /old_locus_tag="NIL17_28295"
     CDS             1317..1847
                     /locus_tag="NIL17_RS28315"
                     /old_locus_tag="NIL17_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1874..2053)
                     /locus_tag="NIL17_RS28320"
                     /old_locus_tag="NIL17_28300"
     CDS             complement(1874..2053)
                     /locus_tag="NIL17_RS28320"
                     /old_locus_tag="NIL17_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2079..2507)
                     /locus_tag="NIL17_RS28325"
                     /old_locus_tag="NIL17_28305"
     CDS             complement(2079..2507)
                     /locus_tag="NIL17_RS28325"
                     /old_locus_tag="NIL17_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2671..2985)
                     /locus_tag="NIL17_RS28330"
                     /old_locus_tag="NIL17_28310"
     CDS             complement(2671..2985)
                     /locus_tag="NIL17_RS28330"
                     /old_locus_tag="NIL17_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2973..3251)
                     /locus_tag="NIL17_RS28335"
                     /old_locus_tag="NIL17_28315"
     CDS             complement(2973..3251)
                     /locus_tag="NIL17_RS28335"
                     /old_locus_tag="NIL17_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3426..3791)
                     /locus_tag="NIL17_RS28340"
                     /old_locus_tag="NIL17_28320"
     CDS             complement(3426..3791)
                     /locus_tag="NIL17_RS28340"
                     /old_locus_tag="NIL17_28320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(3788..4159)
                     /gene="tolA"
                     /locus_tag="NIL17_RS28345"
                     /old_locus_tag="NIL17_28325"
     CDS             complement(3788..4159)
                     /gene="tolA"
                     /locus_tag="NIL17_RS28345"
                     /old_locus_tag="NIL17_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(4433..4678)
                     /locus_tag="NIL17_RS28350"
                     /old_locus_tag="NIL17_28330"
     CDS             complement(4433..4678)
                     /locus_tag="NIL17_RS28350"
                     /old_locus_tag="NIL17_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5005..6690
                     /locus_tag="NIL17_RS28355"
                     /old_locus_tag="NIL17_28335"
     CDS             5005..6690
                     /locus_tag="NIL17_RS28355"
                     /old_locus_tag="NIL17_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6700..6957
                     /locus_tag="NIL17_RS28360"
                     /old_locus_tag="NIL17_28340"
     CDS             6700..6957
                     /locus_tag="NIL17_RS28360"
                     /old_locus_tag="NIL17_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7042..7191
                     /locus_tag="NIL17_RS28365"
                     /old_locus_tag="NIL17_28345"
     CDS             7042..7191
                     /locus_tag="NIL17_RS28365"
                     /old_locus_tag="NIL17_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             5..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7295..7873)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          580..1307
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tggccgcacg cgagcatcat gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg
       61 aacgcgctgg ggacgctgcg cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg
      121 ggcgacttcg gtcagtgcgg gcaggatctg cccgctgatt ttgtgaaaac cacggtactg
      181 gataacacgc cgatccgctg gttctatcgc gcggccagcc aggagtccgc gcagtgggcg
      241 gcgggccaga ccggggagat ccgggtggac gtggagcgcc gccgggccag ccgggaggcg
      301 gggaacgtgg agcacatcag cggcgacagc ttcatccaga aggaggcgcg gccgctgttt
      361 gacgtgaaca ccctgcaaca cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg
      421 gcgcggctgg cgttcagcag cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc
      481 ttcccggtgc tggcgaaaac ggacccgctg gcggagtacc tgcctccggc cgacggcggc
      541 ggaccaaaag cggatgatga ttttgcgggg ctttactgat gacactggag cagtacaacg
      601 tcagccacgc gaagcggcag gcgcggaacg cggagaaaac acggctgacc ctgcgctggc
      661 tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg acggctgggg attgcggcgg
      721 tacagccggt ttaccgcttc ctggacagtc tggtggcgaa agggctgctg gtccgggcga
      781 aatacccggt tgacggccgt caggtcagcg tgtgggggct gacgccgcac ggggtggcgt
      841 tctcgttcga cgaggacgag ccgctgacgg acgttattcc gtttcagccg tcaagggtgt
      901 cggcggcgca gctgccgcac cggctggccg tgcagtccct gcgcctctcc atggagggcc
      961 ggggggccac cggctggcgg tacctgcacc gtatggccct gaaagggatg aaagtgccgg
     1021 acgcgctggc ggagctggac ggcagaacgg tggcatttga ggtagagcgc accgtcaaga
     1081 gccgcaggcg gtatcaggaa gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca
     1141 tcgatgaaat ctggtacatc tgccctgacc ggccgacgct tgtccgggta cagcgggcga
     1201 ttctggcggt ggatgaaatc gtgaatccac agaccggcga ggcccgcaaa acggtggagc
     1261 tggaccggga gaggctgttc gcgtgcttta aatttatgac aacggagtga gggattatgg
     1321 cgctgtacat ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc
     1381 caaccagcga gcatctggaa gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt
     1441 tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg atgtatgacg
     1501 agcggctcaa cagcgacgaa aaagccacgt actttttcac cggtgaaaat ccgcaatggg
     1561 aacgggacaa acccacagga atgaagctgt gtgattacct ctatcgaaag gtggagcggt
     1621 tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg gagcagatta
     1681 acgcgatgat tgaggtggtc agcgagcggc acggggaaag cgcggtgaac gagaagctga
     1741 aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggtg cctgaactgc
     1801 cggaggacaa acccgcccgc cggtggtggc cgttcggcaa aaaataacgc cagcaccggc
     1861 atggccggtg ctgttattct tccccgagct tatcccgcat cacctgccag agtgtttccg
     1921 catcctcgtg aagccgttcg cacatgtcgg ccggggtatc catgtccagc tcgttcagct
     1981 tttccagcaa cagcagcgac tgatccttaa tgaatttagc catattgagg gcagtttttt
     2041 cctgcttcgt catgttctcc attcccttcg gtaagttctc agctaaacca gccggaccag
     2101 aagcgttttt tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc ctggagtttc
     2161 cggcgctcat catcgtgctg gcggcgttct tcttcccggg atgttttatc ttccagcatc
     2221 agggtgacag cctgccggag atcggccagt tccttttgca ttgccagaag ctgttcctgt
     2281 gggaaatttt tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt ctcagggata
     2341 ttcacatttc cgtatacacg gatcagttct gacacatcca cgacagggtt gtttttcccg
     2401 tcacgtgaca cggtaacctt tccctgtttt acatgattgt atagcgttct tctggtaatg
     2461 cccgcggctt gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt atacccctga
     2521 ttttatcggg tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc
     2581 gtttttccgt tcaggatgtg gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca
     2641 cgcaatgaat tgcgcccccg tcatcggtta ttagaggctg tctgcccccg gatacccgga
     2701 ttctttctga tgcctgacgg tgacgatcac aatctggtcc atttcccggt cgtggcggta
     2761 gagcatcaca tagccactgt cgccaaaccc gatcaccagt tccttatatt ccagcggcag
     2821 aaacggcacc gggcggccaa tgtctggcaa tgttttcagt tgctggatag cccggacgat
     2881 cacctcacct ccttttctgg ctgccagccg atttttggtt ttgagaaagt cctgaaggcg
     2941 ttgtaaatcg cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc
     3001 tcgttctcag ttccccaggt acttatccag gcttccgctt cttccgcagt aaggtgcaga
     3061 cccgtttcct gatagtgctg ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc
     3121 tcttcccggt cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc
     3181 cggtcatcgg ccagcgtttt cagccggtcc ttaagttctt catcgagcct gatggatgtt
     3241 gctgatgaca taaaagcccc tttgtagtca tatgtattac acatgactac tttaatccgt
     3301 ggcggcgcta tttgccagaa atacggcttt tgctgcccat ttttcagctt cgcttccact
     3361 aatcagggct gctacaggct cactcatcat ccaggactat tgcagcctgc gggttaaagg
     3421 acctgttact ggcggcaacc atcatcattg agcttttggg tggtgactgg tcgcggatcc
     3481 tgtaagccgt catttttcag actgaattca aaatttataa cctgtcgcat tgccgggtgc
     3541 cctgcgggat atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag
     3601 ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc
     3661 tgaccttcga tgcgttttgc tgcggcctgt accgggtagg ttggctgcag tgtggttacg
     3721 ggttttagtg gtatcgcatg taccggagtg aggaacatgg ccgccagcac tgtcagagta
     3781 aaagctttca tggtctgaaa tccagagggg cgtttttgaa tatttgccag gtattttcat
     3841 ccggtgccgg cggaattttg gcgtgggtaa cggctgatat tgctgcttta cagagtttcg
     3901 gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg gatatccgca
     3961 cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca acagcggtct
     4021 tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa attttcggcg
     4081 gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat gccagcagga
     4141 taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatgtta catgtatatt
     4201 tgtcactctt tacggccacg ttcatttaca taaacacggc ttatcgtatc gtttttacac
     4261 caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg
     4321 cgtttccggc ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg
     4381 ccctcagacc gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc
     4441 ggcgctgtgg ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc cgacgcattt
     4501 cctgcgcccg cctggcttca cgttcgtcct gaatggcacg aagcaccctt tcccgcacct
     4561 gttgttttac cgcctttggc agcgttttgt tccctttcag gcccatggcc agtgcctgag
     4621 cgacaaaggc gtccgccagg cgcttttctt cagcgggagt cagtttcagg tgcgtcatag
     4681 tggcctccgg gcgtctcagg tttcccgaac agtttaaccg caggtcctct cctcatgcca
     4741 gttagctttc agatgtgtct ataccatctg aaatgtaagt gtgatgaatg tcttaaataa
     4801 taaaatttgt tgccatgaga aattttcggg aaaagacgcg atctgcttac aggtggctcc
     4861 gggcgctttt ttgtgcctga agatgagaac taatctcata tattgacacc tgaaaacggg
     4921 aggagtaagg taataattat actgtgtata tgtacagtat gttggcttta atttattgtt
     4981 ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg gtcgaggtcc gggtaattca
     5041 ggtctgggac ataatggtgg tcaggccagt gggaatgtga acggtacgtc tggtaaaggt
     5101 ggcccttcat caggtggtgg tacggatcca aacagcgggc cgggctgggg tacgacgcat
     5161 acgccgaatg gagatattca taactataat ccgggggagt ttggtaacgg cgggagtaaa
     5221 cccggtggta atggtggtaa cagcggcaat catagcggta gctctggtgg cggacagtct
     5281 tcggccactg cgatggcctt cggtctgcct gccctggcta ctccgggggc tgaaggactg
     5341 gctttatcag tttccggcga tgcgttgtcg gccgccgttg ctgatgtgct ggctgccctg
     5401 aaggggccgt ttaagtttgg tctgtggggg attgcgatct acggtgtgct gccttctgag
     5461 attgcaaaag atgatccgaa aatgatgtca aaaattatga cgtcattacc ggccgatgcg
     5521 gtgacggaga ctccggcaag tactttacca ctggaccagg cgacggttcg tgtcagacaa
     5581 cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg cggttgtcgc aggtcggcca
     5641 atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg
     5701 attccgggtc tcccgtctct gcaggtgagc gtaccgaaag gtgttccgac agcgaaagcc
     5761 ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc cggctggttt tacagccggt
     5821 ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga ccggacagaa gccggtttat
     5881 gtgtcggtga cagatgttct taccccggca caggtaaaac agcgtcagga ggaagaaaag
     5941 cgtcgccagc aggcatggga cgccgctcat ccggaagagg ggctgaaaag agactatgat
     6001 aaagcgaaag ctgagctgga tgccgaagat aaaaatattg cgaccttaaa cagccgcatt
     6061 gcatcgacag agaaggcgct ccccggtgca agggctgctg ttcaggaagc cgataaaaag
     6121 gtaaaagagg cagaggcaaa taaagatgat tttgtgactt ataacccgcc tcatgaatat
     6181 ggctccgggt ggcaggatca ggttcgctat cttgataagg atattcagaa tcagaatgag
     6241 aaattaaaag cggctcaggc atctttaaac gcaatgaatg aatccttatc cagagataag
     6301 gctgcactta ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca
     6361 gaaaataagt taaatgagga aaagaacaag cctcgcaagg ggactaaaga ctacggccat
     6421 gattatcatc cagtacctaa aacagaagac ataaagggac ttggagagtt aaagcgaggt
     6481 gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg cccgatggta tggcgataaa
     6541 aaacgtaaaa tttatgaatg ggattcccag cacggtgagc ttgaagggta tcgtgccagt
     6601 gatggcgaac acctaggcgc atttgatccc aaaacgggta agcaggttaa agggccagat
     6661 ccaaaacgga acattaaaaa atatctttga gaggtaaata tggggcttaa attacgtttg
     6721 acatggtttg ataagaaaac tgaagagttt aaaggggaag agtattctaa agactttggt
     6781 gacgatggtt ctgtcattga aagcttggga atgcctttaa aggataatat taataatggt
     6841 gagtttgatt tgaagaaaga atgggttcct ttactacagc cattttttaa aaatcaaata
     6901 aaacctgatg tggataattg ttatatagct tttgattatc gggacggtaa ctggtaacaa
     6961 aaaattatca aggatgattt gaaatactga cttccggtgt tcagggatga atgccggagt
     7021 ctttaaatac agggaaagta tatgaaaaag gtaaagacta tttttctttt tatattgatt
     7081 gttgctggct ttcttctggc cgcatgtcag gcaaattata tccgggatgt tcagggcgga
     7141 acggtggcac catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca
     7201 aaggatcttc ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa
     7261 ccacctgggg aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt
     7321 aactggcttt cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc
     7381 gcacacttca agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac
     7441 caatggctgc tgccagtggc gttttgcgtg ctttcccggg ttggactcaa gtgaacagtt
     7501 accggataag gcgcagcagt cgggctgaac ggggagttct tgcttacagc ccagcttgga
     7561 gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc tatgagaaag cgccacactt
     7621 cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg
     7681 caagagggag cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg
     7741 tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt
     7801 ttatgtgaac tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc
     7861 cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg
     7921 tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg
     7981 cgcaatttcc ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc
     8041 ctgattaagt ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt
     8101 tttcgctgaa cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg
     8161 caagtcatcc ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct
     8221 ggacgccacc gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc
     8281 cacgccagaa attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac
     8341 ggtgctggac gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg
     8401 ctcgctatgg cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg
     8461 ccttttcctc tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc
     8521 atggggcgga aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga
     8581 ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg
     8641 ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg
     8701 gcagtggcat atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg
     8761 ggagtattac agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga
     8821 actctgggcc ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc
     8881 cgatcgctat aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga
     8941 ttcacccggg cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac
     9001 cggcgctgcc ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg
     9061 gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc
     9121 ggctaacctg ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc
     9181 gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa
     9241 cgcgaaactg aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt
     9301 ccgccacctg catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga
     9361 gcaggcccgg aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct
     9421 ggtccctcgt gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg
     9481 caagagcgcg atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt
     9541 ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc
     9601 ggacttcacc ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga
     9661 tctcgaccag tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg
     9721 cgacgtggcg gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca
     9781 gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc
     9841 cctgaaaaag gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt
     9901 cctgcagacg gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta
     9961 cgtcatcggc tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg
    10021 ctgcgcgcaa atcatcattg cgcgggatga gttccggagg
//
