LOCUS       NZ_CP100083             3770 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain RJKP36 plasmid pRJKP36-7, complete
            sequence.
ACCESSION   NZ_CP100083
VERSION     NZ_CP100083.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388314
            Assembly: GCF_025884095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,640
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 249 of 363
            Pseudo Genes (incomplete)         :: 132 of 363
            Pseudo Genes (internal stop)      :: 42 of 363
            Pseudo Genes (multiple problems)  :: 56 of 363
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100083.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RJKP36"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pRJKP36-7"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Ruijin Hospital"
     gene            complement(join(3677..3770,1..101))
                     /locus_tag="NIL00_RS28775"
                     /old_locus_tag="NIL00_28760"
     CDS             complement(join(3677..3770,1..101))
                     /locus_tag="NIL00_RS28775"
                     /old_locus_tag="NIL00_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            385..708
                     /gene="mobA"
                     /locus_tag="NIL00_RS28780"
                     /old_locus_tag="NIL00_28765"
     CDS             385..708
                     /gene="mobA"
                     /locus_tag="NIL00_RS28780"
                     /old_locus_tag="NIL00_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052522663.1"
                     /translation="MTKRSGSNTRRRAICRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1096..1764
                     /locus_tag="NIL00_RS28785"
                     /old_locus_tag="NIL00_28770"
     CDS             1096..1764
                     /locus_tag="NIL00_RS28785"
                     /old_locus_tag="NIL00_28770"
                     /inference="COORDINATES: protein motif:HMM:NF016205.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059034.1"
                     /translation="MSDSGLMMGVITGDLYRSSTDYERGVSYIGLMEVMLEKIKKNNRL
                     RVSDVDFFRGDSFQITSKDPINLLSIAVYIRAYLRSYSEKNDGKGMYDARLAISIDKVN
                     KYRRDINSIFESAQIKSGKSLDLIENDEMMVFDSSLYDLKEIYSPSIILLDALLSMLSK
                     PQSEILREIVYDRDASISEIAERLGKSKQVVYKTIKRGAVDKIIKFLDAHLSLLGMRFF
                     "
                     /name="hypothetical protein"
     gene            1775..2773
                     /locus_tag="NIL00_RS28790"
                     /old_locus_tag="NIL00_28775"
     CDS             1775..2773
                     /locus_tag="NIL00_RS28790"
                     /old_locus_tag="NIL00_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="WP_001477600.1"
                     /translation="MGHGVTVLFLLLLLVHIIFDFYLQTEEMVREKAFDNGIGRALKAH
                     LFHAVMHGFFLCLSCFAFIYVLLGCTNLLNFMLFVLLFSLFTFFHFAIDVAKSITSLKV
                     KGFYCFAVFLFDQVLHILTIFFVIYYFFCDYGVYIYDFNFIKYVGGVLIFACSIAFLLK
                     PSSIIVYLFFKSSGIGDGSEKVKITKSKIAELLYIVLNKELGDSDVKDCKVAVNKYKKK
                     AEEIVIGISDGQADIDIRTISKVNNAGRFIGYLERLIIFLFLILGSVTAVVAVLAMKTA
                     LRFSDLKDDNDSGKAEYIMIGTFFSLIVTVIISVLARMYLIEIGIKINDFS"
                     /name="DUF3307 domain-containing protein"
     gene            complement(3041..3190)
                     /locus_tag="NIL00_RS28860"
     CDS             complement(3041..3190)
                     /locus_tag="NIL00_RS28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_162847635.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPGRHDKAPLAAAIDKSRCARDFR
                     ECLK"
                     /name="replication initiation protein"
     Typing          137..376
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          complement(3119..3642)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.528"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          386..705
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="1.44e-61"
ORIGIN
        1 tgttcgtcag catcgagtgc gtccagtttt tcgagcagcg tcaggctctg gctttttatg
       61 aatcctgcca tgttgagtac ggcttgctgc tgcttgttca tcttttcgtt ttctccgttc
      121 tgtctgtcat ctgcgttgtg tgattatatc gcgtaccact tttcgactgt tttaccgccg
      181 gtattctgcc gtctgacgct ttgacgggta tttctgcctg acaatactgt cactgccaaa
      241 aaactgccgt gtctttgtcg gtaattcgaa cttgctgaca ggacaggatg tgtaattgtt
      301 ataccgcgca tgcatgcacg ctattacaat taccctggtc agggctttgc cccgacaccc
      361 atgtcagata cggagccatg ttttatgaca aaacgaagtg gaagtaatac gcgcaggcgg
      421 gctatctgtc gccctgttcg tctaacggca gaagaagacc aggaaatcag aaaaagggct
      481 gctgaatgcg gcaagaccgt ttccggtttt ttacgggcgg cagctctcgg taagaaagtt
      541 aactcactga ctgatgatcg ggtactgaaa gaagttatga gactgggggc gttacagaaa
      601 aaactcttta tcgacggcaa gcgtgtcggg gacagggagt atgcggaggt gctgatcgct
      661 attacggagt atcaccgtgc cctgttatcc aggcttatgg cagattagct tcccggagag
      721 aaaactgtcg aaaacagacg gtatgaacac cgtaagctcc caaagtgatc accattcgct
      781 ttcatgcata gctatgcagc gagctgaaaa cgatcctgac gcatccttcc tgttttcccg
      841 gggtaaaaca tctctttttg cggtgtctgc gtcagaatcg cgttcagcgc gtttcagcgg
      901 tgcgtacaat tatggcttta tgttaaatgc agggtgacaa ctgaatttta atattgagcg
      961 ccttagagcc aagaagtatc aagttattgg tgtaaaccaa aatgggttac ttttttgttt
     1021 ttcaaccttt ttggttgact attttccttt tcggttgtgc aatctgtcaa gaattatttt
     1081 agtttggggg atgaaatgag tgattcaggg ttaatgatgg gggttatcac tggtgatttg
     1141 taccgctcga gtaccgatta cgaacgaggg gtttcttaca ttggcttgat ggaggttatg
     1201 ttagaaaaga taaagaaaaa caataggtta agagttagtg atgttgattt ttttcgaggg
     1261 gattcatttc agataaccag caaagatcct attaatttat taagtattgc cgtttatatt
     1321 agggcttatt taagatcata tagtgagaaa aacgatggta aagggatgta tgatgctcgc
     1381 ttggcgatat ccatagataa agttaataag tatagaaggg atataaactc aatatttgaa
     1441 tctgctcaga taaagtcagg gaaaagttta gatctcattg aaaatgatga gatgatggtc
     1501 ttcgactcat cactttatga tttaaaggaa atatattccc catctattat tcttcttgat
     1561 gcattgcttt caatgttgtc caaacctcaa tcagaaatcc taagggagat tgtatatgat
     1621 agggatgcaa gtatttctga aatagcagaa agactgggaa agagtaaaca agttgtttat
     1681 aaaaccataa aaagaggagc ggttgataag ataataaagt ttttggatgc tcatctttca
     1741 ctgcttggaa tgagattttt ttaatggagg gggtatgggg cacggtgtaa ctgttctatt
     1801 tttgttgttg cttttggttc atataatttt tgacttttat ttgcaaactg aggagatggt
     1861 tagagaaaag gcattcgata atggcattgg tagggcttta aaggctcact tatttcatgc
     1921 cgtgatgcat ggtttttttc tatgtctatc ctgcttcgct ttcatatatg tactgcttgg
     1981 ctgtaccaat ctattaaact ttatgctatt tgttttgctg ttttctttat ttacattttt
     2041 tcattttgca atagatgtcg caaagtcgat tacctctctg aaagtaaagg gtttttattg
     2101 ttttgctgtt ttcttgtttg atcaagtttt gcatatttta actatatttt ttgttattta
     2161 ttatttcttt tgtgattatg gcgtatatat atatgatttt aatttcataa aatatgttgg
     2221 cggggtgttg atatttgctt gttctatagc gtttttacta aagccttcat ctattatagt
     2281 ttacttattc tttaaatcat caggtattgg tgatggttca gagaaggtta aaataaccaa
     2341 aagtaaaatt gcggaattgc tttatattgt attgaataaa gaactgggtg attctgatgt
     2401 taaagattgc aaggtggcag taaataaata caagaaaaaa gcagaggaaa ttgtaatcgg
     2461 tatttctgat gggcaagctg atattgatat cagaactatt tccaaagtta ataatgctgg
     2521 tagatttata ggttaccttg aaagattgat aatttttttg tttttgattt taggctcggt
     2581 cactgcggtt gtcgcggtgt tagcaatgaa aacagctctg agatttagtg atctaaaaga
     2641 tgataatgat agtgggaagg ctgaatatat aatgattggt acatttttta gtcttattgt
     2701 aaccgtgata atatctgtgt tagccagaat gtacttgatt gagattggta taaaaattaa
     2761 tgattttagt tagtttgagc tcagtttctc acataagaag atgatggttg caatgatgag
     2821 aaatgatatc aatggaatga tgatgcattt tcgtatctga gaaggatctt cttgagatca
     2881 tttatatctg cgcgtaatct cttgcactgg aaacgaaaaa accacctggg gaggtggttt
     2941 ttcgaaggtt aagtcagttg gggaactgct taaccaggta actgtcttgt gcgagacgag
     3001 ccaccaaaac tgttctttta gtgtagctgt agtccggcca tcacttcaag cactctctaa
     3061 aatctctcgc acatcgactc ttatcaatgg ctgctgccag tggcgctttg tcgtgtcttc
     3121 ccggattgga ctcaagtcga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga taccaacagc
     3241 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3361 tttaagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3421 aggggggcgg agcctatgga aaaacgtctg cggcgctggc ttcttccggt gctttgcttt
     3481 ttgctcacat gttctttccg gctttatccc ctgattctgt ggataaccgt attaccgctt
     3541 ttgagtgagc tgacaccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     3601 aggaagcgga agagcgcctt atgtgacatt ttctccttac gctcctctat gccgttctgc
     3661 atcctgtccg gatgcgttat atcccggtgc gattttccgc ttcaaagcgt gtctgtatgc
     3721 tgttctgaag ttcttccgcg agttcgtgca gcttctcaca catggcggcc
//
