LOCUS       NZ_CP100082             4510 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain RJKP36 plasmid pRJKP36-6, complete
            sequence.
ACCESSION   NZ_CP100082
VERSION     NZ_CP100082.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388314
            Assembly: GCF_025884095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,640
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 249 of 363
            Pseudo Genes (incomplete)         :: 132 of 363
            Pseudo Genes (internal stop)      :: 42 of 363
            Pseudo Genes (multiple problems)  :: 56 of 363
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100082.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RJKP36"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pRJKP36-6"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Ruijin Hospital"
     gene            complement(233..589)
                     /locus_tag="NIL00_RS28745"
                     /old_locus_tag="NIL00_28730"
     CDS             complement(233..589)
                     /locus_tag="NIL00_RS28745"
                     /old_locus_tag="NIL00_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(793..1113)
                     /locus_tag="NIL00_RS28750"
                     /old_locus_tag="NIL00_28735"
     CDS             complement(793..1113)
                     /locus_tag="NIL00_RS28750"
                     /old_locus_tag="NIL00_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1296..1538
                     /locus_tag="NIL00_RS28755"
                     /old_locus_tag="NIL00_28740"
     CDS             1296..1538
                     /locus_tag="NIL00_RS28755"
                     /old_locus_tag="NIL00_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            complement(2080..2526)
                     /locus_tag="NIL00_RS28760"
                     /old_locus_tag="NIL00_28745"
     CDS             complement(2080..2526)
                     /locus_tag="NIL00_RS28760"
                     /old_locus_tag="NIL00_28745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(3313..3723)
                     /locus_tag="NIL00_RS28765"
                     /old_locus_tag="NIL00_28750"
     CDS             complement(3313..3723)
                     /locus_tag="NIL00_RS28765"
                     /old_locus_tag="NIL00_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3723..3914)
                     /locus_tag="NIL00_RS28770"
                     /old_locus_tag="NIL00_28755"
     CDS             complement(3723..3914)
                     /locus_tag="NIL00_RS28770"
                     /old_locus_tag="NIL00_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4034..4443
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcgctcctc caagccagtt accttggttc aatcacctgc aagtgtttga accttcggaa
       61 aaaccgccct gcaaggcggt ttttccgttt acagagcaag agattacgcg taccgaaacc
      121 ggatctcaag tgctcgtaaa agaaaatgat gatgaccttt agcggttaca aaaaaagaag
      181 gcacactatt acagaggcct tcttctagtt tggttaaatt tttttaaaaa aatcatgcga
      241 ccgcatgttt tttttccaaa gattcagcat tgtgcttcct ttctataatt tcatgagcgg
      301 gagtgactct ttctatttct tcctgaaggc gtgggctgct cgtaacctcc cacaaatcaa
      361 cagactgctg tatatttaac cagaacttag cggtagtatc gaatgcttta gctaaccgaa
      421 acgccatatc ggtagacagt ttcctattat tgttaaccaa agcacttgca gaatttctat
      481 gcacgtttaa catttcagcc aagtcattga tttttaaacc acttggctcg agatattcat
      541 aaagaataat atctcctaca gtcgatggtt ttctggttgc ttgggacatg ctttactcct
      601 gaattggtcg cttctctctg ttagatgagg aagataagta tttcttctgt taccagctgt
      661 atcgattagt aattatctta aatatagtta cgtcgcgtta ctttattaat cattatttgt
      721 gatgacgttt taacgtaggt gaggaaatgc ctcacctact tgttttgtaa gataacagtt
      781 ccggtctgtt acctatcggt aatcatgtgg atcgagatac aaatcctcga ctccgttctc
      841 accccactta aatatcaagc ggtacttatc attcaccctt atagatgaat accctgtcaa
      901 tggagggttg agattttcaa atcggttccc cgggggagac cgtaaatctc caattttatc
      961 agcagcattc agcatatcca gcttgcgacc taatgaaaca ctaatcgtag ctggaatttt
     1021 tcgattggct ttaccgtatt tataaaactg gtatagccaa tcgtctcgga actgaatgcg
     1081 gcaatcatca tcactatcat gattttgctg catttttctt ttacctcaaa tgttgccgca
     1141 acttggcaag ttctagatca ccttttacca ccaccatagt acaacaaacg ccagaaatgc
     1201 acaatagcac tgtgcacttg tgcaaaaaac cataatgata taaaatgcac aaaagcacag
     1261 tgctactgtg caaaaaaaga atagttggag gtcatatggc gcttgttcgc tcaggaaata
     1321 aaaccaagat ttttgaagca gatccgaaat ttaacccagt ttttgataca caacacaagc
     1381 ctggcgatac gtgcacgtat gaaggaattt acaaatgcac aggttgcggt aaagaagtaa
     1441 ctatcgcagg agggaaaaca cttccaccac aaaatcatca tcaacatagt cccggacagg
     1501 gggacatccg ctggcaacta tttgtttatg cagagtgagg tatgaaaatg gcagaccgta
     1561 gaccacttaa cccgcatgtc gtcgtcccta aaccaggagg cggaactcag ccacgagatc
     1621 gtaataataa tggaagagtc cgagagaaac gttcagatgc gggaaaatca agaaaataag
     1681 aaaagccgcc aaataggggt cagtttggat atcggaaact tttgtacgtt aagcgcatta
     1741 tttaaccaat attgatgcgt aagggattga ttttacctca gctacgatcg cttttaagtc
     1801 accgctcttt ctgtatcgca tttaggttat ccgattaagg aaaattcacg ggaaacggcg
     1861 tatagcgcgg gagtacatca ttgcgagagg taagcctgtt caaaacacag gcttaccgta
     1921 caataattct ctatatccaa actgacccca agaagtctga cactaacccc acacaaatcg
     1981 aatccgttgc aagatgagac acacaagaaa agagctagac gaaggtcgct ccgctcccca
     2041 tggtttttgt atacccacca tgggggcata ggtagtttat taactaactc caagataatg
     2101 cgtgatcgca gccgtgagta caccacagcc cacttgttta gataaatcta aaaccccttg
     2161 taggattctc ttagtgttcc actcaccttc tgcatccatt ggttgagata gtgcagcact
     2221 caactggttc ttaatctttt gcgaagttat atcgttatct attgagttga tttcctcctt
     2281 ggctttctcc aaggaagcct ggagttcacc gcgttctatt tttaactttt cacgataact
     2341 gtctggtgtg tgtagttcgt tcccatccgc atcgagatta ataacttcgc ctcgctcacc
     2401 aacagtaaca aagctctcta cgtgagacag atcatcaaca accatcacat tccttttcat
     2461 tcttaaacca gtcacttttg cacctgcacc gatcttcata aaactaaagc ctgatttatc
     2521 gttcactcac atcacctcat aagataaaaa tgttaataac gttttcacga gcaattattt
     2581 accataacgc ctcattacgc gcaccgctca aacgctctag aaacgattct ggggcgtaat
     2641 ccattaaatc ctacgtctta ccccctgaaa acgcctcaaa atcgctccag ggcgttttac
     2701 gggcatgaat tgttatgcac cagactgact ggtggcggat ttcagcctga attcaggcca
     2761 gacaactcaa aacccgcacc aaaccagact gaaaacggtg aacggggacg caatttattg
     2821 cgtggccgtg aagcgttgcc ggcgcagccg gaaaaacggt tttcagcacg ttcaggaatc
     2881 gggttcgtcg acggccggca atcacgttat gcaagtcgcc ggacgactca ccacacgatt
     2941 caccggcgat tcgactcgtg acttgactct agagtcgcca aggtcaaaaa aacaccaaaa
     3001 tgacgattcg actctagagt caagtcgtca taagttactg atagtcttaa gtgagtcgtc
     3061 gatgactcta tgacgagtcg tcggcaagtc gtacacgaat cgccgacgac ttggattcac
     3121 cttacagaaa gtaatactgc gtaatgtaat aacccacaac taactgatat taatgaatta
     3181 tcttcaaaat tttaattttg cgtggggtgt gggtattttt agtggtgagg agcgttcaga
     3241 gatctggcga tttttgggaa ggttcaggaa ctccccggga tggccggaga ggaaaacgga
     3301 agcatgacgg agtcaggatt ttttccagaa tttccaccac ggggaatcgg gtggtgaaga
     3361 aggttcgtgc ttgtgctcca gaagctgcat agcctgacga agggaatcaa tatgctcatc
     3421 tcgagcctta acagcccctt taagaccatc gttttcagct tctagtaaag caattctatg
     3481 ttgtaaatca tgtaaaccaa cgctgttaac acctgtagat cctgcgtcac tttttacagt
     3541 atcgatgaca ccatcaagag aaccaaaaac tctaataagc tctgaagtat caataacttt
     3601 aatgatcttc ccgtgcttta caacagaggt gtaacttaac ttcccactgt taatcatctt
     3661 gtaaattgtg gggcgactaa ccttaacaag ccgtgccgct tcactaatag acactctggc
     3721 catcagttac catcctgtaa ttcatccagt ctcgaggaca gtgtacggaa aagatgctcc
     3781 gcatcatcgt ggagtttctc acaaaggtcg gcctctgcat caagatcaag ctcgttaagc
     3841 ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag cgctgttttc
     3901 tgttgtttcg tcataagaag cctcagagcg tcagtgctca gtgtaaaacc agtctctttt
     3961 ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggtagga
     4021 ctatgcgtat aaatgctcga gaaaaccgag cggacgcgtg atgggcagaa ggaaaggccg
     4081 cttagcggcc gtttttccat aggctccgcc cccctgacaa gcatcataga aattgacgct
     4141 caaatcagtg gtggcgaaac ccgacaggac tataaagata tcaggcgttt ccccctgatg
     4201 gctccctcgt gcgctctcct gttcctgcca ttcggtttac cggtgtcatt ccgctgttat
     4261 ggccgcgttt atctcattcc acgccagaca ctcagttccg ggaaggcagt tcgctccaag
     4321 ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgtcttatc cggtaactat
     4381 cgtcttgagt ccaacccggt aagacatgca aaagcgccac tggcagcagc cactggtaac
     4441 tgatttagag gagttagtct tgaagtcatg cgcctgttaa ggctaaactg aaagaacaag
     4501 ttttggtgac
//
