LOCUS       NZ_CP100080             9730 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain RJKP36 plasmid pRJKP36-4, complete
            sequence.
ACCESSION   NZ_CP100080
VERSION     NZ_CP100080.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388314
            Assembly: GCF_025884095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,640
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 249 of 363
            Pseudo Genes (incomplete)         :: 132 of 363
            Pseudo Genes (internal stop)      :: 42 of 363
            Pseudo Genes (multiple problems)  :: 56 of 363
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100080.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RJKP36"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pRJKP36-4"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Ruijin Hospital"
     gene            complement(join(8660..9730,1..1119))
                     /locus_tag="NIL00_RS28660"
                     /old_locus_tag="NIL00_28645"
     CDS             complement(join(8660..9730,1..1119))
                     /locus_tag="NIL00_RS28660"
                     /old_locus_tag="NIL00_28645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            complement(1116..2201)
                     /locus_tag="NIL00_RS28665"
                     /old_locus_tag="NIL00_28650"
     CDS             complement(1116..2201)
                     /locus_tag="NIL00_RS28665"
                     /old_locus_tag="NIL00_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            2578..2841
                     /locus_tag="NIL00_RS28670"
                     /old_locus_tag="NIL00_28655"
     CDS             2578..2841
                     /locus_tag="NIL00_RS28670"
                     /old_locus_tag="NIL00_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2831..3130
                     /locus_tag="NIL00_RS28675"
                     /old_locus_tag="NIL00_28660"
     CDS             2831..3130
                     /locus_tag="NIL00_RS28675"
                     /old_locus_tag="NIL00_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3192..3569)
                     /locus_tag="NIL00_RS28680"
                     /old_locus_tag="NIL00_28665"
     CDS             complement(3192..3569)
                     /locus_tag="NIL00_RS28680"
                     /old_locus_tag="NIL00_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            3638..3817
                     /locus_tag="NIL00_RS28685"
                     /old_locus_tag="NIL00_28670"
     CDS             3638..3817
                     /locus_tag="NIL00_RS28685"
                     /old_locus_tag="NIL00_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3845..4375)
                     /locus_tag="NIL00_RS28690"
                     /old_locus_tag="NIL00_28675"
     CDS             complement(3845..4375)
                     /locus_tag="NIL00_RS28690"
                     /old_locus_tag="NIL00_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264279109.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAHHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4382..5113)
                     /gene="mobC"
                     /locus_tag="NIL00_RS28695"
                     /old_locus_tag="NIL00_28680"
     CDS             complement(4382..5113)
                     /gene="mobC"
                     /locus_tag="NIL00_RS28695"
                     /old_locus_tag="NIL00_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(5113..7074)
                     /locus_tag="NIL00_RS28700"
                     /old_locus_tag="NIL00_28685"
     CDS             complement(5113..7074)
                     /locus_tag="NIL00_RS28700"
                     /old_locus_tag="NIL00_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     Typing          7875..8455
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4386..5113)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 aacacgacca aataaagcat agtccccatt aagcgacagg tcatagcttt tggaattata
       61 ttccctcagt gatccccata cttgctgata catactttgt cgggtatctt tatgccggta
      121 tgttgccgtc cctaacaggt cagcatttct ccagttttgc agcgttgaaa atttcagctt
      181 ccagctatta ctgaacttct gctcaagagt tgcaaatgca gttttacttt tacttctttc
      241 aaactcccag tcattgcctg taaacgtcga gcgaggaaga ttaaggtggt gtccggtacc
      301 agaaataggt aagtcatacc accaatgatt gttatattca tactgctgat atcccccaat
      361 cgtaagtagg gtattatcag tcagatctgc ctccataatt ccgtaaatga cgttattcct
      421 gcgtttctca ctatccctga aggtctcact gttttcgcca tccacaactg cccttccgcg
      481 taaggtcgcg ctgtcattca gtggtcctga tacatcaacc ccaccccggt agtcattcca
      541 ccgtccggca ccgatgttag tgctggcctg atattgccgg gtcggacgct tgtgtatcat
      601 atttatcgcg gctgaagggt ctccggcacc ctgaacaaga cctgttgccc cgcggactat
      661 ctccacccgg tcaaaaatag ctgaactagt ttgtgaactt ggaatgtatg aattccacgg
      721 caggctaaat ccttcataca tgacattagc attaaatcct ctggacataa aattcggtct
      781 tccggaacct tctgctgtac tgacatataa tccaggagcg gttttaacca catcaataat
      841 attttttgct gctacatcat ccattcttcg tcgcgtaatg actgtagttg actgaggtgt
      901 atcccggaat gacagattca tctttgatgc tgtattggct gactgggttg tgtatgaatc
      961 tgttccttct gttgtgcttt cagaaacagc ggtcccatga acaattaatg tttcttctgg
     1021 tgaaatattt ttatctttac taactgcgcc agaatcatca gccataacag atgatgtata
     1081 catcatatat actatgctca gccctaaata tttattcatt cttctggctc catagcaatt
     1141 ttagataatt cgattttgag tatgtaatcc ttcatagtac tcatctcttt tttttcttgt
     1201 tttacactaa cgtacagatt ttcaccgtca tgtgataaag acagactgtt tggcatcagg
     1261 cgggttttta tcgttgtttt tacgtaataa gaattactgt ccacaatgct gacctgtttt
     1321 gcattacggt gcgtgatata gatttcgttc cgtaaaggat taaataaaat cgccagtgaa
     1381 tttataacat caattctgtg tagtattttt cccgagttta catcaacaac cagcaccccg
     1441 ggaatatccg ggtcggtaat aaatgcacgt ccgttttttt catccagact tacattaagg
     1501 aaaaagtgtt ttttctcagg ctctacaaca aaccgttttt ttatttcata gctactggca
     1561 tccaggttaa taatctcacc atccccgtta accagataaa tattatcccc atttttattt
     1621 aacgcaattc cagtaacagc gtgccccagt ccatcaatgg ttttaatttt cttcccgaca
     1681 gccatgtcaa taacccacag agccccttta tctgcggcgc tggagacata aagacgctga
     1741 ttttttttgt caatgaccat ttctctggtc ggtctgaaat gagcctcatc ctgtccctca
     1801 gttaacggaa ttactgaaag ttcttttccg gttagagtat caatgaccgt cactgagccg
     1861 tccagagtat tgccgatata aagtcgctca tgttcctcat ccagtgctgt ggcaaaccca
     1921 agacgtgcga ttttaacggc agattttgtt ttcagagtgg atggttctaa ctgataaaca
     1981 attccgtggc ccttatctct gtcgaatgaa gacgatgatg ctgcccagag actattattt
     2041 ttgccatcaa aagacatttc atacagacca ttgccgatat cacgagtcag gaatgttgat
     2101 tcagctatat cagccgcata cgctgaacca gtaaacagta gtaaagagta tattaatact
     2161 ctgctacgga cattaacggc ggatttaatc gtgattgcca tacttacatt tccttacaat
     2221 aaaagaaaca agaatatagc aaaagtgata atgattatca ctaccattaa tcacatgatt
     2281 taccataacg tcgcattacg cgcaccgctc agacggtctg agggcatttc cggcggctac
     2341 gggataaaac cggcaacaaa ccacccggaa aaccgccgga aacgcgcctg ataaatttta
     2401 acccgctgca tggctatgca cgcaactgca tgtcgtgcag tttgacgtaa aatgggtgtc
     2461 gtaagccctt ttttaccagc tttcgtcttc atcagcctga ctgacacgag catattcttt
     2521 cagaccgccc cctgctaaac tccttcatat cgacgtatca gcccgggagg ctccacaatg
     2581 agcaacagta cacactatga aaacgccaat ttcctgcggg aactggccga gagactgccc
     2641 cgcatactgc ctgaaggtgg cccggacaaa gctgcactgt tgcagcgcct ggctaacgaa
     2701 gaactggccc aggcagaata tgaagatcag gttcgcgcta aagtgaccgc cgcccgcgct
     2761 gacacgcgcc cgggcatgac cacggaacaa ctccgccaga gactgcatgg ccgctatcag
     2821 gaactacgcg atgccgtatg aggtggaatg gaagcaggga gccatcgagg atgtgacagc
     2881 attgtttgac tacattgcgg aaaattcatc cctgtgggat gcgcagaacg ttaccgatcg
     2941 agttctcgct gcagcggaca aactggccga cttcccgcgc ctatatgaag tggactcacg
     3001 ctatggcgag gacgttcgcc ggatcagtct gatgggacag catgtcctct atgatgttga
     3061 tgaccagacc aggaaagtcc gggttctggc cgtggttggc cagcgccaga atccccatac
     3121 cgttcgctga taaccggtga tgggggcgca attcattgcg tggccgtcgc cggttggaca
     3181 agcacagcgc gtcagcgttt tccccactgc caccatcggc gcgatgttcc ggccggtgtc
     3241 tctgctgtct ctggtttgtg ttccagcagt ctcatggcct gcatttcctg ccgcagtccg
     3301 gccacctcct ccctgagtgc ttttatctcc gcgatcaacg cgtcggaggc cattttttca
     3361 gtctcatccg acagtctcaa cccgtcccgc tcgtgagtct caatctgtct taattcaccg
     3421 tacacccgga taagttcgct ggtatccacc acccgaccac catcagcttc agtacggtat
     3481 gatacgcggc ctttcgccat atccctatac agggaactcc gggctttccc cgtcagttcc
     3541 gtcgcctgtc tcaccgtcac ccacgccatt gtctcacctc cggttttgag acagtatacc
     3601 cgacaggaag cacaccacac ccggaaggac agaaaacatg actaagcagg aaaaaactgc
     3661 cctcaatatg gctaaattta ttaaggacca gtcgctgctg ttactcgaga aactgaacga
     3721 gctggatatg gataccccgg ccgacatgtg cgagcggctg cacgaggatg cggaaacact
     3781 ctggcaggtg atgcgggata agctcgggaa ggaataacag caccggcatg gccggtgctg
     3841 ccgtttattt tttgccgaac ggccaccacc ggcgggcggg tttgtcctcc ggcagttcag
     3901 gcaccggcac gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct
     3961 cgttcaccgc gctttccccg tgccgctcgc tgaccacctc aatcatcgcg ctaatctgct
     4021 ccggtgagca cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca
     4081 ccttgcgata gaggtaatca cacagcttca ttcctgcggg tttgtcccgt tcccattgcg
     4141 gatttttacc ggtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca
     4201 tcccgatgga tttcatcatc gccagatgat gggcccggtc gacaaaacag aaaacatacg
     4261 ccccggtctg ccaggcgagc accaccatat cttcaagatg ctcgctggtc ggcatgatgc
     4321 agcgctcgcc gtcccggtcc tccgcgtctt caataaatac catgtacagc gccataatcc
     4381 ctcactccgt tgtcataaat ttaaagcacg caaacagccg ctcccggtcc agctccgccg
     4441 ttttgcgggc ctcgccggtc tgtggattca cgatttcatc caccgacagg atcgcccgct
     4501 gcaccctgac ctgcgtcgcc cggtccgggc agatgtacca gatttcatcg atgccgttcg
     4561 ctttgcgatt gaacagatac ccggacacca cctcctgata gcgcctgcgg ctcttcacgg
     4621 tgcgctccac ctcaaacgcc accgttctgc cgtccagctc cgccagcgcg tccggcacct
     4681 tcatcccctt cagggccata cggtgcaggt accgccagcc ggaggccccc cgggcctcca
     4741 tggcgagacg cagggactgc acggccagcc ggtgcggcag ctgcgccgcc gacacccttg
     4801 acggctgaaa cggaataacg tccgtcagcg gttcgtcctc gtcgaacgag aacgccaccc
     4861 cgtgcggcgt cagtccccac acgctgacct gacggccgtc aaccgggtat ttcgcccgga
     4921 ccagcagccc tttcgccacc aggctgtcca ggaagcggta aaccggctgc accgccgcaa
     4981 tccccagccg tcgcgcaacc agctccgccg tggaacacag ctcttcccgc agccagcgca
     5041 gggtcagccg ggttttctcc gcgttccgcg cctgccgctt cgcgtggctg acgttgtatc
     5101 gctccagtgc catcagtaca gccccgcaaa atcatcatca cccggccgct gccgcccggc
     5161 ctccggctgg tactccgcca gcgggtccgt tttcgccagc accgggaagc gctgcagcgg
     5221 gatttcccgg cgttcaaccc gcagcgggct gctgaacgcc atccgggcca cgcccagccc
     5281 ggtcatcacc gcaaacccgt caggcaggtg ctgcagggtg ttcacgtcaa acagcggccg
     5341 cgcctccttc tgaatgaagc tgtccccgct gatatgctcc acgttccccg cctcccggct
     5401 ggcccggcgg cgctccacgt ccacccggat ttccccggtc tggcccgccg cccactgcgc
     5461 ggactcctgg ctggccgccc ggtagaacca gcggatcggc gtgttatcca gcaccgtggt
     5521 tttcacaaac tcagcgggca gatcctggcc gcactgcccg aagtcgccca gcgactggtg
     5581 cgcgagcagc aggttgcagt cccgactgcg cagcgtcccc agcgcgttca gcacgtactt
     5641 cgacagcagg tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc
     5701 atcccgcgca atgatgattt gcgcgcagcg ggcaaacagc atcttctgca cccggatcac
     5761 cgcctcatcg tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc
     5821 cacgtcgatg ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt
     5881 gatcagcccc ttcacgtcct ttttcagggc ctccggcagc gcgtatgccg ccgccagcac
     5941 ctgctgaatg tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc
     6001 gctgatgcgg tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag
     6061 cagattcttc agggcgtact ggtcgagatc gcgaaacaga ttcagctgcg ccggtttccc
     6121 cggccgcaga tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca
     6181 cgcgtcccct ttggggtcga agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat
     6241 gagcgcgttg gtgcccatca cgctcttgcc gctcccgggc aggccgagga tctgaatatt
     6301 ggtcttgcgc caggtatcac gcgggaccag caccggctgg ccctgctcat ccttacccag
     6361 aaacaccgcc tgctgtttcc gggcctgctc tgtggaaaaa tcggccagcg cgttaaccgg
     6421 taccggctcc ccgaaatgca ggtggcggac gtcggtcatt tctccggtcc gctggctgct
     6481 gcgccggctc tcctggttca gtttcgcatt caccgcctcg tcagcggccg caaaatgacc
     6541 gtcaagcagc agaaatgcca gcagccgcgc cagaaggatg ccggcgacaa gccctccggc
     6601 acaccagaca atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcgta
     6661 gaggtccgga cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc
     6721 ccccagaagg gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgccg
     6781 ccgtgccctg accatccgcc cgggcgaatc cagcgaagcc agcaccgaaa atggcgcagt
     6841 ccggttatta tcgcgcttat agcgatcggc atactcccgt cgctccttat gactcagccc
     6901 cacaaaacgc atccgtccgg cccagagttc agccagaccg aaaaacaggc cgataaccag
     6961 gaaagccccg gccagaccgt aatactccct gtacagccag ctgacccacg ggtaaatctg
     7021 ccgggccagc tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc
     7081 tctctccttc agacgacggg tttaccggcg tggtgtgcag cccctgctgc acaagccgcg
     7141 gacatgcccc gaggcatggc ggcggcggtc acgtccgtgg tgattgcatt cagctttcac
     7201 cacggaggcc agcctctttc cgccccaagc ggaaaaggcg cagccccgga ggaacaacga
     7261 gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc
     7321 ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt
     7381 tgctgcttcg tcaggtgcgt tcagcaccgt gggtttttgt gggtggtcag gcgtgagcct
     7441 gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccgagctgag ccagaaagag
     7501 ggggaaagac cgtgcgtcgg tggcgtccag ccagcgtaag caggggattt cctcctgaag
     7561 tcagccacgc cccagcgggg atgacctgca tttctccgca aggagaaaaa agcaacagag
     7621 ataggttaac gtaaaatatt cagcagaaaa ccgacagact tcacctatct gaatgattta
     7681 tcggcaggga agggaaaggc ggtaacacgg tgcaatatgt cacatccgcg cgtccggtgg
     7741 ccgtctgcgg gaggggggat attgcgcctc ctccctcact cagtcgctac gctccggccg
     7801 tgagactgcg gcgggcgctg ctaactctcg caaagttacc cacagattcc gtggataagc
     7861 aggggactaa catgtgaggc aaaacggcag ggccgcgccg gtggcgtttt tccataggct
     7921 tcgccctcct gccagaattc acataaacag acgctttttc ggtgcatctg tgggagccgt
     7981 gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac
     8041 cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg tttaccggat
     8101 acctgttccg cctttctccc ttacgggaag tgtggcgctt tctcatagct cacacactgg
     8161 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga actccccgtt
     8221 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc ggaaagacac
     8281 gcaaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc
     8341 taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt
     8401 tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa
     8461 ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgacg
     8521 aaccatggag gatctcaaga agatcctcta catgatttga ttaaccatca actgaagtgg
     8581 caaacagggg gttgtacgcc ggaaccacaa aagacggatt tataggggca aatccgagga
     8641 gaagacccaa tccgcatggt tagaatctat aggttgcact aattttaaca tttcttggtt
     8701 ctccatatgt ttccgtgcca tatccaatcg attcacctat ggatgtataa taaaccttat
     8761 caaagacatt agttatattg agctgtaaat ccagttgtgg cattggagta tagccaataa
     8821 taatgtcagt tgttgaataa gccttttgtt tattaataac cggattgcca tcccttgtgt
     8881 ctgttatgtt agcatatatt ttactttgcc agtacgtgtt tactcctgct cgccagtttg
     8941 ttaattttcc gtcaaaggtg tacatgctcg aaatcttaaa taaattttgt ggcgacacgg
     9001 cagtattgaa tcgcttgcct tcattactac gatctccatc ttttatatat cgcgtatctg
     9061 catatgtata tcccacccca atttcccaga gtggcgtaac catgccctga agttcgaaat
     9121 ctatcccttt tgtgcgtact ttaccgctag cctcatagca ttgaggattg ccattagtaa
     9181 cacaccctgt ctgatcggga agcagcctgg ctcgattttc ctggtctacc tgaaataccg
     9241 ccagagaggc attaagcaag ccgttaaaat actcaccctt aatacctgtc tcataattct
     9301 taccaacaat aggcttgatc actttaccat tgaggtcggt gcttgactgt ggattgaata
     9361 tatcggtata gctcccataa atggtatgat gatcgtcaag attgtatgtt gaacctgcat
     9421 atttggtgat atttctggta actttataac ctgtattaat ggatgtacct ttacttgtat
     9481 attcatacca gtcaagacgg ccacccagca atattttcca gttatcacta agtagaaaat
     9541 gagttgctgc gtaggcgctt ttttgttcag tgacgtcgta ggatgcatca cctgacatct
     9601 tcatgggttt tttcgaagaa tgtgaaccat gatcaaatat attaacattc tcaatatata
     9661 ttggggaata tgtataacct gtatctttat cggtgtattt ttgctgagtt gttccagcca
     9721 cgaattgatg
//
