LOCUS       NZ_CP100075             5596 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain RJKP41 plasmid pRJKP41-3, complete
            sequence.
ACCESSION   NZ_CP100075
VERSION     NZ_CP100075.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29388315
            Assembly: GCF_025884075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Plasmids carrying blaKPC-2 tend to evade CRISPR immunity in ST15
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hu,Y., Jiang,J., Wang,D., Guo,Q. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:01:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,550
            CDSs (total)                      :: 5,425
            Genes (coding)                    :: 5,278
            CDSs (with protein)               :: 5,278
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 59 of 147
            Pseudo Genes (incomplete)         :: 90 of 147
            Pseudo Genes (internal stop)      :: 25 of 147
            Pseudo Genes (multiple problems)  :: 24 of 147
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP100075.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RJKP41"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pRJKP41-3"
                     /geo_loc_name="China"
                     /lat_lon="31.02 N 121.04 E"
                     /collection_date="2018"
                     /collected_by="Ruijin Hospital"
     gene            547..771
                     /locus_tag="NIL01_RS27665"
                     /old_locus_tag="NIL01_27655"
     CDS             547..771
                     /locus_tag="NIL01_RS27665"
                     /old_locus_tag="NIL01_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            755..1021
                     /locus_tag="NIL01_RS27670"
                     /old_locus_tag="NIL01_27660"
     CDS             755..1021
                     /locus_tag="NIL01_RS27670"
                     /old_locus_tag="NIL01_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1091..1441)
                     /locus_tag="NIL01_RS27675"
                     /old_locus_tag="NIL01_27665"
     CDS             complement(1091..1441)
                     /locus_tag="NIL01_RS27675"
                     /old_locus_tag="NIL01_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1507..2052)
                     /locus_tag="NIL01_RS27680"
                     /old_locus_tag="NIL01_27670"
     CDS             complement(1507..2052)
                     /locus_tag="NIL01_RS27680"
                     /old_locus_tag="NIL01_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2078..2404)
                     /locus_tag="NIL01_RS27685"
                     /old_locus_tag="NIL01_27675"
     CDS             complement(2078..2404)
                     /locus_tag="NIL01_RS27685"
                     /old_locus_tag="NIL01_27675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2762..2920)
                     /locus_tag="NIL01_RS27770"
     CDS             complement(2762..2920)
                     /locus_tag="NIL01_RS27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3560..3736
                     /locus_tag="NIL01_RS27690"
                     /old_locus_tag="NIL01_27680"
     CDS             3560..3736
                     /locus_tag="NIL01_RS27690"
                     /old_locus_tag="NIL01_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3726..4070
                     /locus_tag="NIL01_RS27695"
                     /old_locus_tag="NIL01_27685"
     CDS             3726..4070
                     /locus_tag="NIL01_RS27695"
                     /old_locus_tag="NIL01_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4279..>4713
                     /locus_tag="NIL01_RS27700"
                     /old_locus_tag="NIL01_27690"
                     /pseudo=""
     CDS             4279..>4713
                     /locus_tag="NIL01_RS27700"
                     /old_locus_tag="NIL01_27690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4748..5257
                     /locus_tag="NIL01_RS27705"
                     /old_locus_tag="NIL01_27695"
     CDS             4748..5257
                     /locus_tag="NIL01_RS27705"
                     /old_locus_tag="NIL01_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5264..5476
                     /locus_tag="NIL01_RS27710"
                     /old_locus_tag="NIL01_27700"
     CDS             5264..5476
                     /locus_tag="NIL01_RS27710"
                     /old_locus_tag="NIL01_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3395..3474
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2812..3209)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4061..5592
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaggaaa acagggttct catcgggcat ttacgctgaa accggatgtc attcacctgg
       61 ctgcatggct atgcagccag gtaaaattta tctgtcgcga acggggcggt ttatcgggtg
      121 gtttgttgcc ggttttacct gactgccacc ccgttcgcgg cgaacccgtc cggggcggtg
      181 cgggcaacgg gtgttatgtt taatcagcgt gaacggcaga tacaaaaaag ccccggaggg
      241 cttttaacta ctgacttatt tttttgaaga tttgctgggt ttcaatttgt cagtatttcc
      301 cggcgttttt ttattgtcac gctggcgttt atcaggttcc cctgttgaat cggcaattcg
      361 tttcggaccc ggcttaagac ccataatcgt tatcctttca atagttaaga ggaaagccct
      421 caagctagat atattgaccg cgttacggat tatcaagagg ggctaacaag attatttttt
      481 gttcttttag gttgattgtg gtactgttga tatacatgta tagcaaatgg tgttcgggag
      541 gtttatatgc ttgctatccg gttatctgat gagattgagt cccgtctgga ctcgctggcg
      601 aagcaaaccg gcagaacaaa gacgttttat gcgcgggaag caatactggc gcatctggaa
      661 gacctggagg attattatct ttcagcagaa actgctgcac gcgttcgccg tggtgatgaa
      721 gcagtgcatt cgtctgaaga cgtgaggaag tcacttggtc tggacgatta actattccga
      781 tcgggcgctc aaatcgttac gcaagatgga caaacagaac gcacgacgga ttgtggattt
      841 tatgagttta cgcattgcag ttgctgccga tcctcgccag tcagggaagc cgctcaaagg
      901 tgagctgggc gagttctggc gctatcgggt gggagattat cgcgttctgt gtgagatccg
      961 agatgacgag cttgttatcc ttgccgccac gattggacat cgccgcgaag tttacgactg
     1021 aagcccgcta tgcgggcttt tttgatgccg acaacaggta ttatggtaaa tactcgacta
     1081 actacgtgtt ctactgtttg tcggttttag ttaaccagca ggttgtctta acgataatgt
     1141 agccgttatt tttaactaat aaggtcatat tgttaacgtc aggcataata aaagacatta
     1201 tggcctcaga gttatcatca gaagcagctc tctgcattac tacttcgatt cccttttctt
     1261 tgaatctata agtatccagt tgcctattaa acttagaaag acctacaacc atacttagtt
     1321 tgttatcttt aacagtaaga gatgcttcct gagtaccatt acatcggtag taagcggtgt
     1381 aaccgtcagc aaaagatcga ccggggaata taaatgctag gaacgcaatt aaccaataca
     1441 tagttgagaa tcttggtatt acacatttca ctatcctctc tttcattttt cgttcaccat
     1501 actttgttat ctgattttta atttttctat tttataaacc aagtctctac caaaaaggcg
     1561 aaaattatcc tcaaataata ataattgttt aagaacatcc atgaaagcag ctgcttcacc
     1621 ctcagttatg ggagctactc tatataacat ataacctgag ttaagggttt cttttatact
     1681 ttcttcaatg ctcattataa atattagtgg attatcattt ttcttccccc atgggtttct
     1741 aattaatcct tcgatgtact caggtccttt gcgtcctatt gcctgcgcaa tattttttat
     1801 ttcatctgcg tgagggcttt gttgtacagc tttgtttatg cctgcttgag tgattttcag
     1861 agactctaca ccgtgtatgt tgtatgagat aaaatgtaat aaattaagat gcaatgcaat
     1921 taatccattt ttgtttacat ctgagtattt ggataaaaaa cgtaatgaag tgttaactct
     1981 cccttgaagc ctaacaattg ggagtgctgc cttgtatatt tccttctccg ttaaataccg
     2041 tttaaaaaac ataattctcc gcctccgtat agcgtgccta ttttatttcg tcgtaaaact
     2101 tagctctgaa atattccttt agaaccatca tgtcattaat aattgaatct atctcatcca
     2161 ttttttcttg gtattctttt aatggtgaag ggagcggtaa ctcaatattt gaaaagtgat
     2221 tggcgagata gggatcaagg tttctcatcc attcaggctt ttcgccaatg cgttcttcaa
     2281 ctgaaagtga acctgataat ctctggttaa taagatcact tagactcatt ttggttttat
     2341 tcgaaagccg ttgtaaggat tgctttaatc ctatatctag catgatattt gttctttctc
     2401 tcattacccc cccttgctat tgcgtttttt gttgcgcaca aagctaccat tgcgcattaa
     2461 gaaacgcaat aaaaaaggag ggagggagtg gtttgtacat gatgtgcagt gcgctgaggg
     2521 catcagtggc gggtgttttt ccgtgggatg gatggagctc cggggataac agaactgacg
     2581 atcatttaaa ggatcttctt gagatccttt ttttctgcgc gtaatctctt gcactgtaaa
     2641 cgaaaaaacc gcctggggag gcggtttgat cgaaggttaa gtcagttggg gaactgctta
     2701 accgggtaac tgggtatacg agaccgcaga taccaaaact gttctttcag tgtagccgca
     2761 gttaggcacc cacttcaaga cctctcaata tctctctcgt atatatccag ttaccaatgg
     2821 ctgctgccag tggcgttttt gcgtgtccgt ccgggttgga ctcaagatga tagttaccgg
     2881 aaagggcgca gcagtcgggc tgaacggggg gttcgtgcat acagcccagc ttggagcgaa
     2941 cgacctaaac cgagccgaga taccagcagc gtgagctatg agaaagcgcc acgcttcccg
     3001 aaagggaaaa aggcggaaca ggtatccggt aaacggcagg gtcggaacag gagagcgcac
     3061 gagggagccg ccggagagaa atcaccggca tctttaagtc ctgtcgggtt tcgtcctcct
     3121 ctgacttgag cgtccatttc tgtgatgctc gtcaggggag gcggagccta tggaaaaacg
     3181 gcaacgccgc atccttttct ccggcccttt ctgatttgag cgcctttgct ttatcccgtt
     3241 atccgtgagt cggtcacgcc cgccgcagcc gaacggcagg agcgcagcta ccgagtgagc
     3301 gaggaggcgt catttcatct gtccgtgcat ggtattgcgg acgctcctga gccacatatc
     3361 acacctgcca catgaggcac tttcatgaat cgtgctctca tgccaacata gtaagccagt
     3421 atacacagcg taaacgctgt gactggttca gggctgcgcc ccgaaacccg ctaacaccac
     3481 tgacgcgctg gcgcttgtcc aacgccggag cgacccggaa aacttttccg tgccgtccgg
     3541 cgtttgcgaa ggagggataa tgcagaagcc gaagaagaag ccgctcgtcg taaaaaaaag
     3601 atcctggcat ccggcagtga tggcaaatct gatgcgtcag gcacaggagg aagattaccg
     3661 ccgggagcag atggcagagc ttgagcgttc agcccggtca cattttgcgg atatcggagg
     3721 agaggatggc ggttaagcgt acccgttttt taggtatccg ggtgaccgat ggggagtacc
     3781 agcagctgct ggagcgctgc aatggaaggc aactggcggt gtggatgcgg gaaacctgtc
     3841 tcgatacgcg cccggcgcgg tcgttgcggc tgccgtccat cgatccggtt ttgctgcgcc
     3901 agcttgccgg gatggggaac aacctcaacc agatcgcccg gaaaattaac ggcggccagt
     3961 ggtcaggcgc tgaccgggtt caggtggtgg ccgcgctgat ggccattgat gccgaactcg
     4021 agcggctgcg gcatgccgtg ctggaaaagg gggcagacga tgatcgttaa gtttcatccg
     4081 cgagggcgag gcggcggtgc cgggccggtg gattatctgc tggggaaaga ccgccagcgc
     4141 gacggggcca tcgtcctgca gggaaagccg gaggaggtcc gggagcttat cgacgcctcg
     4201 ccctacgcca aaaagtacac ctccggcgtc ctgtcttttg ctgaacagga tttaccgccc
     4261 ggccagcgtg aaaagctgat ggcgagcttc gagcgggttc tgatgcccgg actcgataaa
     4321 gaccagtaca gcgtgctgtg ggttgaacac cgggacaagg ggcggctgga actgaatttt
     4381 ctggtaccga acacggaact gctgaccggc aggcgtctcc agccgtacta cgaccgggca
     4441 gaccgtccgc gcatcgatgc gtggcagacc gtggtgaacg ggcgactggg gctgcacgac
     4501 ccgaacgcac cggagaaccg gcgcgcgctg gtgacgccat cttcgttacc caaaacgaag
     4561 caggaagccg cagaggctat tacgcggggc ttactggccc tcgcctcgtc aggggagctt
     4621 aaaacgcgtc aggacgtcac tgaggcgctg gaaagcgcag gttttgaggt cgtgcgcacc
     4681 acaaagagca gcatcagcat tgccgacccg gacggagggc gaaacatccg acttaaggga
     4741 gccatctatg aacagtcttt taacgctggc gaaggactta gagcagaaat cgaaagcgca
     4801 gcagcagagt accggcgaaa tgctgaaagc cgcattcagc gagcacgaga agtctgtcag
     4861 agcggaactg agcgaaagcg ggaagagaat cagcgccgcc atccgcgccc acgagcaggg
     4921 tatgagcgcg gccatgcagt cgaaccgcct gagcgtgatg cgtatggtcg ggcggacatg
     4981 gctgaccatc acgatggtgt caggactgct gttcgccagc ctgagcgggg tgttgtggta
     5041 tcaggggagc ttgatagcgt caaatctggc cgaaatcgac cggcagaacg cagcgctgtc
     5101 gaagctgaac gcgaagacct ggggcgtgac gtacctggaa gacagcaacg gacgttttct
     5161 ggtgctgccg aaggggacgg ccgtagacag gacgcagagc tggacggtcg ggaacgggcg
     5221 gtcgaagcag aacgcgctga gactggtgaa ggagtaagtg ccgatgaccg agctggaaaa
     5281 acagttgctg agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg
     5341 ggagagcgcc ttcggggaat ggcggaaaat gtcagggctt atgcaacggg agaacgcggc
     5401 gctgagcgag cgcgtgacgc gcttgagtca gcaggtggaa cgcttgagcg ggcaactgca
     5461 gcgtttgagc cggtagtgca gcgtcatgag atggccgtgg ccgcagaacg ggcgcacgag
     5521 caacggcagc acgaaaagga actgacggaa gcgcgttccc ggcagcgaag ctatgacggt
     5581 ccgtcgctgg gctgaa
//
