LOCUS       NZ_CP099745            20453 bp    DNA     circular CON 29-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 503666-2-2019 plasmid p503666-2-2019c,
            complete sequence.
ACCESSION   NZ_CP099745
VERSION     NZ_CP099745.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29187370
            Assembly: GCF_025232415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 20453)
  AUTHORS   Fernandez,J.E., Seth-Smith,H.M.B., Nordmann,P., Egli,A.,
            Endimiani,A. and Perreten,V.
  TITLE     Intra and inter-species spread of a novel conjugative
            multidrug-resistant IncC plasmid co-harbouring blaOXA-181 and armA
            in a cystic fibrosis patient
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20453)
  AUTHORS   Fernandez,J.E. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2022) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/29/2023 07:33:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,622
            CDSs (total)                      :: 5,501
            Genes (coding)                    :: 5,368
            CDSs (with protein)               :: 5,368
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 56 of 133
            Pseudo Genes (incomplete)         :: 79 of 133
            Pseudo Genes (internal stop)      :: 22 of 133
            Pseudo Genes (multiple problems)  :: 20 of 133
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099745.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20453
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="503666-2-2019"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p503666-2-2019c"
                     /geo_loc_name="Switzerland: Basel"
                     /lat_lon="47.5617 N 7.58339 E"
                     /collection_date="2019"
                     /collected_by="University Hospital Basel"
     gene            complement(107..826)
                     /locus_tag="NHF44_RS26975"
                     /old_locus_tag="NHF44_26960"
     CDS             complement(107..826)
                     /locus_tag="NHF44_RS26975"
                     /old_locus_tag="NHF44_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="WP_017879874.1"
                     /translation="MLLRCLLPLALLPLAAVASAACTLTDPTLTLQSYRVDAQKERIAM
                     YWQDRHGKAWGSLRSLLAGIDGDGRVQMAMNGGIYDKAYAPLGLYIEDGKQLTPVNRSA
                     GGGNFFIRPGGVFLVENGRAKIVPLPAYKPSPAIRYAVQSGPMLIENGLINWRLKPSAS
                     SRKLRNGVGIDKQGRVVFMLSDRETNFYDFACYAQSKLGVRQMLYLDGTLSKMYRKGGS
                     VPWQYHPFVTMITVERK"
                     /name="phosphodiester glycosidase family protein"
     gene            complement(874..1989)
                     /locus_tag="NHF44_RS26980"
                     /old_locus_tag="NHF44_26965"
     CDS             complement(874..1989)
                     /locus_tag="NHF44_RS26980"
                     /old_locus_tag="NHF44_26965"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225698.1"
                     /GO_function="GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="WP_002893127.1"
                     /translation="MPLADFHRSDPFTLGIELELQVVNPPGYDLSQDASTLIADVQHEL
                     TVGEAKHDITESMLEIATGVCRDISHAQIQLSAIQQAVQRAALRHHLQICGGGSHPFHA
                     WQRQQISDNPRYVKTVEHFGYLAQQATVFGQHVHVGCQSGDDAIYLLHGLSRFVPHFIA
                     LNAASPWFDSTDSRFACSRLNRFSSYPDNGPMPWVADWQGFRRLFRQLSYTSMIDSMKD
                     LHWDIRPSPQFGTVEVRVMDTPLTLAQAIHIAGFIQTLACWLLTERPFKHQPDDYLLYP
                     FNRYQACRYGLDGTLTDVRSGEQRSIRQEILQLADRLAPFAHQLKATAALEAVVRQAKS
                     PQSEAQQMRDFIANGGSLSGLVQKHCEIWAA"
                     /name="YbdK family carboxylate-amine ligase"
     gene            2316..3983
                     /locus_tag="NHF44_RS26985"
                     /old_locus_tag="NHF44_26970"
     CDS             2316..3983
                     /locus_tag="NHF44_RS26985"
                     /old_locus_tag="NHF44_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_004893615.1"
                     /translation="MHKKTLFSRAGRLAVAVSGALALAGAALPANASTLRIAMTAADIP
                     LTLGQPDQGYEGNRFTGIPLYDALVEWDLSQGEKPSGLVPGLATEWHIDPQNTSRWIFT
                     LRPGVKFHDGTEVNADAIVWNVDKVLNKAAPQYAPGQIGNTLSRMPTLTGAEKIDDHTV
                     ALTTSEPDALLPYNITNLFIVSPTAWQKQYDAVPASVRDSAARSKQAWTAFAAHAVGSG
                     PFKLEKLVPRQQLILDKNPDYWNKDRIPRVDKVVLIPLPEANARTAALLSKQVDWIEAP
                     APDAIDQIKSQGFHLYANTQPHLWPWQFSFEKGSPWQDIRVRKAANLCLNRAELKSYLG
                     GYMTEATGVYEADSPWHGKPTFQIKYDPDAARQLMTEAGYNASKPLHVTVATSASGSGQ
                     MQPLPMNEYIQQSLKSCFFNVDIKVVEWNTLFTNWRLGARDPSAKGIDAINVSAAVNDP
                     YFGLIRFSTAKAFPPVATNWGYFSTPETEKLAAAVKHAFTPAEMNKAAGELHAALVDQV
                     PFLFVAHDVGPRAISPAVTGVVQPQSWFIDLSLVSKKE"
                     /name="ABC transporter substrate-binding protein"
     gene            4002..4955
                     /locus_tag="NHF44_RS26990"
                     /old_locus_tag="NHF44_26975"
     CDS             4002..4955
                     /locus_tag="NHF44_RS26990"
                     /old_locus_tag="NHF44_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012968749.1"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_004893611.1"
                     /translation="MINTLLYRILLAIPTMLGVAVICFMLVQIAPGDPLVSVMPPDASE
                     ALRQTLMQAYGFDKPLPLQFIHWLWRALHGDLGMSVATGRPVIDEVMTAVAYSLRLALL
                     ATAIGFVLGSLFGFVAGYFRNSVIDRLASVLSVFGVSVPHYWLGMLLVILCSVKFALLP
                     ATGGGPIGEVGWQWDWAHLQFMLLPALTLSVIPTGIIARTVRSQVADILSQEFIVGLRA
                     RGLNESRIFVHVMKNAAPTALAVMGLQVGYLMGGSILVETVFSWPGTGLLLNTAIFQRD
                     LPLLQGTIWVLALFFLLLNLLVDILQTTLDPRIKRS"
                     /name="ABC transporter permease"
     gene            4958..5851
                     /locus_tag="NHF44_RS26995"
                     /old_locus_tag="NHF44_26980"
     CDS             4958..5851
                     /locus_tag="NHF44_RS26995"
                     /old_locus_tag="NHF44_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805546.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_004893608.1"
                     /translation="MVDTATTKAVPAAAAHATRQGYWRGVLRRLLRDPGGVVVGVVILL
                     LLALALLGPWLIVKDPYQTSMFLRLKPIGSDGFPLGSDELGRDMLSRLILGTRLSLFMG
                     IVPVVFAFFIGGAIGIIAGYTGGKTNTVIMRTVDVFYAFPSVLLAIALSGALGAGIGNA
                     LLSLTLVFVPQVARIAESVTAQVRHMDYIDAARATGASALTIIRVQVLGNVLGPIFVFS
                     TGLISVCMILASGLSFLGLGVRPPEPEWGLMLNTLRTAIYTQPWVAALPGLMIFITSIS
                     FNILADRLRAAMAIKE"
                     /name="ABC transporter permease"
     gene            5855..6892
                     /locus_tag="NHF44_RS27000"
                     /old_locus_tag="NHF44_26985"
     CDS             5855..6892
                     /locus_tag="NHF44_RS27000"
                     /old_locus_tag="NHF44_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899021.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_004893606.1"
                     /translation="MQPFINIDLGGPAQPLLKVNHLLKYFRGGKGREVVQAVDDISFTV
                     MKGETLGVVGESGCGKSTTARLLMQLLTQDSGELIFDGQGVGSSRLPLKAYRRQVQMVF
                     QDSYASLNPRLTMEESIAFGQRVHGVSAREASEYARYLLAHVGLEPARFADRYPHALSG
                     GQRQRVNIARALAMKPRLVILDEAVSALDKSVEAQVLHLLQDLKRTLELTYVFISHDLH
                     VVRWLSDRILVMYLGEVVEIGPAEQLFTASAHPYTRALLSSMPSMDPENRTLTSPLSGD
                     PPSPIAPPSGCRFHTRCPHAKAVCAEVKPRLEAVGEGHQSACLMAQPASPWHQSIPLKE
                     VSHVG"
                     /name="ABC transporter ATP-binding protein"
     gene            6882..7892
                     /locus_tag="NHF44_RS27005"
                     /old_locus_tag="NHF44_26990"
     CDS             6882..7892
                     /locus_tag="NHF44_RS27005"
                     /old_locus_tag="NHF44_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899022.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_004191439.1"
                     /translation="MSDNAFVHVRDLRVVFRRDGQTINAVNGVSFEVQKGEVMALIGES
                     GSGKSVTLRALMRLHPPGSSELSGTLQVGDDEVLTMSASQLRRYRGGRCAMIFQEPLLA
                     FDPVYTVGQQIIEGLRRHEGLSRQAARDRALEALRQVRIPSPERRLDAYPHEMSGGMRQ
                     RAMIALALSCNPQLLLADEPTTALDATVQIQILILLREQQKQRGLSIVFVTHDIGAAVE
                     IADRVAVMYAGRIVEEASMSEILRHPRHPYTQVLLGSRPKEGLKKGDALHCIPGAPPDL
                     ARLPPGCAFAERCPHRRPVCEQSLPPTESVAERHAVSCHRWRELAIDANAQALAV"
                     /name="ABC transporter ATP-binding protein"
     gene            7990..9258
                     /locus_tag="NHF44_RS27010"
                     /old_locus_tag="NHF44_26995"
     CDS             7990..9258
                     /locus_tag="NHF44_RS27010"
                     /old_locus_tag="NHF44_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="WP_004893599.1"
                     /translation="MSSDVLFTPATDTTGWRINGDRLWASLMDLAQIGATPKGGCRRLT
                     LTDLDRQGRDKVIGWAREAGMSVTIDKIGNVFMRREGRNPGLPPIVSGSHIDTQPTGGK
                     FDGNYGVLAALEVVRTLNDLQIDTDAPIEVVFWTNEEGSRFVPVMMGSGVFAGVFPLEE
                     TWAVTDKEGVSVGEALEQIGYIGEQTPGDHPIGAYFEAHIEQGPILEDEAKTIGIVQGV
                     LGIRWYDCVVTGQASHAGPTPMRLRQDALQVATRIMQEVVAIAGRSEEGRGTVGSVQVW
                     PNSRNVVPGEVTFSIDMRNLSDALVDEMDRQLRAFIAEVERESGLQVALKQVSHYPAAP
                     FDAECQQAIADAAQRLGYPARPIVSGAGHDAVYMSYLAPTGMIFIPCKDGISHNEIEYA
                     SPEHVAAGANVLLQVMLQYARPV"
                     /name="Zn-dependent hydrolase"
     gene            complement(9362..10270)
                     /locus_tag="NHF44_RS27015"
                     /old_locus_tag="NHF44_27000"
     CDS             complement(9362..10270)
                     /locus_tag="NHF44_RS27015"
                     /old_locus_tag="NHF44_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004893597.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_004893597.1"
                     /translation="MDKSAPGPWSGWVHGLLGVIIFSGSLPATRLAVQDMDPLLLTFLR
                     ASIAGLLAIALLVGFRQKRPRLAQLVSLIIVSSGVVVGFPLLTALALQRITSAHSIVFI
                     GLLPLMTALFGVLRGGERPRRAFWIFSLLGSLLVVGFALTQNAAASLSGDLLMLAAVIV
                     CGLGYAEGAKLTRELGGWQVICWALVIALPLMLPASLLVQPASWHAISASSWAALGYVS
                     LFSMLIGFIFWYKGLAAGGIAAVGQLQLLQPFFGLGLSAALLHETVSPLMLAVTLGVVL
                     CVVGSRKFARQRVGAGSGSRD"
                     /name="DMT family transporter"
     gene            10395..11729
                     /locus_tag="NHF44_RS27020"
                     /old_locus_tag="NHF44_27005"
     CDS             10395..11729
                     /locus_tag="NHF44_RS27020"
                     /old_locus_tag="NHF44_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225700.1"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_004893594.1"
                     /translation="MKARYKAVVDRYAQAIRSGQLPAGSRLPTHRTLAAGERLSLATAT
                     RVYRELEEMGLVSGETGRGTFVRDLSLPPGHGVDQQVVAADVVDLNFNYPSLPDQGDAL
                     REALRQLAMAGDIDSHLRYQPHAGRLAERDIIARHLTCQHFAPDAENVLIVNGAQHGLA
                     VTVMGLLRPGDVVAVDALTYSGFKVLAALYHLELAAIPCRPEGPDLQALQTLCQQRRVR
                     AVYTMPTLHNPLGWVLNTGQRQALADLARQHDLLIIEDAAYARLVSHPPPPVVSYAPER
                     TVYVTGFSKNIATGLRVGVVISPPRYRPEIERAIRATTWNTPTLISSLICAWIEDGTVA
                     RFETQKRQDARQRQQVARELLCGLPVVSHPDSYFVWLPLGEESRADRLANALMERRISV
                     STAEPFCVSATIPQALRIALGSVPFDSLRPALLSVRDAVEYEQYR"
                     /name="PLP-dependent aminotransferase family protein"
     gene            complement(11726..13189)
                     /locus_tag="NHF44_RS27025"
                     /old_locus_tag="NHF44_27010"
     CDS             complement(11726..13189)
                     /locus_tag="NHF44_RS27025"
                     /old_locus_tag="NHF44_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225701.1"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_004893593.1"
                     /translation="MIRHLLHVDFQPGRGLQEQVRETLVNAILSGIFAADTPLPSCRQL
                     ASQLRVSRNTTALVFESLVNEGYLISRPRSGYYLHPDYHEASPAAVESAPQREAAAPRW
                     GDRLQMTPSQQESILKPAGWMHYRYPFIYGQPDTRQFPLATWRSAANWLHGGVRDPAWV
                     IDHIDQDVPMLIEQIRTRVLPKRGIVAAPDEILITLGSQNALYLLTRLLLSSTTRIGVE
                     NPCFREAINTFLLADAEVVPHPVDEEGIVLNERPCDYYYVTPGHQVPTGVTMSSARRRQ
                     LLEHATRHDAVIIEDDYDSESNFTLNPLPALKASDRSGRVVYVSSLSKALSPGLRLGFM
                     VADPDLIDEARALRRLVYRHPPTNIQYQMAHFLAQGHYETHLRRYHYDSAQRWECLHAA
                     LQRYLPSCRALAGSEHANAFWLQTPAQINTQQLTWRAAHAGVLIEPGARHFLNAAPPDN
                     YFRMGFHAINPDAIAQGVEVLRGQLEQMG"
                     /name="PLP-dependent aminotransferase family protein"
     gene            13431..14282
                     /locus_tag="NHF44_RS27030"
                     /old_locus_tag="NHF44_27015"
     CDS             13431..14282
                     /locus_tag="NHF44_RS27030"
                     /old_locus_tag="NHF44_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_004176946.1"
                     /translation="MKKSLASMCLSAVLTVAFSYHAVAADLPEIEKSGTLKVATEDDYA
                     PFNFMNNGQADGFNKDMLEELRKYAKFHVDQSILPWTGLLAAVSTGQYDMALTGAVITD
                     ERLKVFDFTPPWASAQHYFVKRAGDTSLNTIADLSGKKVGVQAGSALLARLPELKAMLE
                     KTGGKLGPVVEYPSYPEAYADLANKRLDYVINVVISVNDLAKAKPKVFAKGLAVSGPGY
                     MAWPIPKNSPQLLAYMTRFMNHMKETGKLAELQKKWFGETYDNLPTEAITSPEQFHKLA
                     GL"
                     /name="transporter substrate-binding domain-containing
                     protein"
     gene            14330..14971
                     /locus_tag="NHF44_RS27035"
                     /old_locus_tag="NHF44_27020"
     CDS             14330..14971
                     /locus_tag="NHF44_RS27035"
                     /old_locus_tag="NHF44_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225703.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_002893187.1"
                     /translation="MDAISWQLLIEGAWTTLWISAIAIAFGVVAGLLIALVRMLRLPVI
                     DQLLVVYISLARATPLVTLVLFLFLSLPTMGINLDKNVAAIVALTLNTSAFNAEIWRNA
                     FRTFPREQREAAESVGMRRWTYFRYIMLPQMWIESLPALVNEMSFLIKGSPAIAVIGVV
                     DLTRVTNRISSVTYEPLSPILAAGLLYVAIIGCLLKLQGIAERKARRLAR"
                     /name="amino acid ABC transporter permease"
     gene            14986..15651
                     /locus_tag="NHF44_RS27040"
                     /old_locus_tag="NHF44_27025"
     CDS             14986..15651
                     /locus_tag="NHF44_RS27040"
                     /old_locus_tag="NHF44_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225704.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]; GO:0005886 - plasma membrane [Evidence
                     IEA]"
                     /GO_process="GO:0015837 - amine transport [Evidence IEA]"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the opines
                     (in Agrobacterium) octopine and nopaline, etc.; The
                     N-terminal region of this protein, as described by
                     TIGR01726, is a three transmembrane segment that identifies
                     a subfamily of ABC transporter permease subunits, which
                     specificities that include histidine, arginine, glutamine,
                     glutamate, L-cystine (sic), the opines (in Agrobacterium)
                     octopine and nopaline, etc.; The N-terminal region of this
                     protein, as described by TIGR01726, is a three
                     transmembrane segment that identifies a subfamily of ABC
                     transporter permease subunits, which specificities that
                     include histidine, arginine, glutamine, glutamate,
                     L-cystine (sic), the opines (in Agrobacterium) octopine and
                     nopaline, etc; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_017879875.1"
                     /translation="MNQWGVIWSVRDSFIAGLFATLELFITAALAALIIGIALCYFSEY
                     QKKVINRIIIGFVSLMRAIPFLILAYLLYYGLPQLGISMEPWTAGLLSLIIYHGAYFFE
                     ILRSQRRVFSGGYIEAAIAQGFSRYAIFRRIILPNIVSSALPLIGNQLIICLKDTAFLS
                     IITVQEITAAANSVQATYFIPFNAFIVAIGLYWAISILLELLIKRLTAWGAKRGMSHA"
                     /name="ABC transporter permease subunit"
     gene            15644..16405
                     /locus_tag="NHF44_RS27045"
                     /old_locus_tag="NHF44_27030"
     CDS             15644..16405
                     /locus_tag="NHF44_RS27045"
                     /old_locus_tag="NHF44_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225705.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_004151676.1"
                     /translation="MPESIAVSVKNVSKQFDNVEVLRDINLTVEKGTVVSILGSSGSGK
                     STLLRCMNWLEQPDRGEIHIGGQRLGIDEQRGRAMSHRQLAKIRERVGMVFQSFNLWPH
                     LTVQQNVSEALLRVKGMKRDEAKAMAMQQLEKVGMAHKADVYPITLSGGQKQRVAIARS
                     LAMSPEVILFDEPTSALDPELVNEVLGVMKALAAEGYTMVVVTHEMDFARQVSNEVVFL
                     EKGLLIEKAPPEKFFTQPDSERVRQFLQSSR"
                     /name="amino acid ABC transporter ATP-binding protein"
     gene            complement(16459..17223)
                     /locus_tag="NHF44_RS27050"
                     /old_locus_tag="NHF44_27035"
     CDS             complement(16459..17223)
                     /locus_tag="NHF44_RS27050"
                     /old_locus_tag="NHF44_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_004893579.1"
                     /translation="MATSNVVFITGATSGFGEAAAQVFADAGWSLVLSGRRYPRLKALQ
                     DRLAAQVPVHIIELDVRDSEAVAAAVASLPADFADITTLINNAGLALSPLPAQEVALED
                     WKTMIDTNVTGLVTVTHALLPTLIRHGAGASIINIGSIAGQWPYPGSHVYGASKAFVKQ
                     FSYNLRCDLLGTGVRVTDLAPGIAETEFTLVRTKGDQAASDKLYRGTTPLSARDIAEQM
                     FYIATLPAHMNINRVEVMPVRQAWQPFAIDRD"
                     /name="SDR family NAD(P)-dependent oxidoreductase"
     gene            17404..18741
                     /locus_tag="NHF44_RS27055"
                     /old_locus_tag="NHF44_27040"
     CDS             17404..18741
                     /locus_tag="NHF44_RS27055"
                     /old_locus_tag="NHF44_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="WP_004214933.1"
                     /translation="MKLAIHNEVAVSNDEVRQLDRAYVFHSWSMQGNLNPLVIAGAQGC
                     ELWDYEGNTWLDFSSQLVNVNIGYQHPRVLAAMKSQLETLVTIAPATANLARGEAAKRI
                     VDLAPAGFSKVFFTNAGADANENAIRMARLYTGRDKVLSAYRSYHGNTGSAIAATGDWR
                     RVPNEFSRGHVHFFNPYLYRSEFNAATEEEECQRALAHLRRIIECEGPTAIAAILLESI
                     PGTAGILVPPAGYMQGVRALADEFGIVLILDEVMAGFGRTGSWFAFEQDGVVPDLVTFA
                     KGVNAGYVPAGGVLISEPIARYFDDHFFAGGLTYSGHPLAMAAIVATIDAMKEEKVVEN
                     AASIGNEVLRPGLEALAEKHAIIGEVRGRGLFQALELVSSREQKTPLTAADMAAIKGAL
                     TEAGLLAFVVENRIHVVPPCTITAEQVAQGLAIFDAVFARFASLAK"
                     /name="aspartate aminotransferase family protein"
     gene            19058..20421
                     /locus_tag="NHF44_RS27060"
                     /old_locus_tag="NHF44_27045"
     CDS             join(19058..19526,19526..20421)
                     /locus_tag="NHF44_RS27060"
                     /old_locus_tag="NHF44_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611151.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISKpn1 family transposase"
                     /protein_id="WP_085955203.1"
                     /translation="MAKPKYSPETKLAVVNHYLSGKDGEQSTADLFGIERTSVRRWVRA
                     WQFHGAEGLTAKNNHYSDEFKLVVVRAVISDRLTMREAAARFNLSAEILVRRWLDVYND
                     AGAEGLLNMQCGRPGQMTKPKNIPPLTDKELEKLSPEELRAELRYLRAENAYPKKVESL
                     GSERKKWQKALIISELRHEHALRDLLRAAGMSRSTWYYNMNALKQGDRYAGLKENIRKI
                     YHYHKGRYGYRRITLALRKQGLRINHKTVQRLMAELSLRSVIRAKKYRAWKGRTGEAAP
                     NILSRNFGASKANEKWVTDVTEFPVQGKKLYLSSVLDLFNREVIAYSLSERPVMEMVNT
                     MLDGAFPKLRPGDAPLLHSDQGWHYRMRSYQERLKAHGMTQSMSRKGNCLDNAVMENFF
                     GTLKSECFYLREFRSVSALRKAVEDYIHYYNNERISLKLKGLSPVEYRTQALRAA"
                     /name="IS3-like element ISKpn1 family transposase"
ORIGIN
        1 gcagcgccac cgggcaagga catcgggcac ggcggcaata ccttgcccgg cctgtcgtta
       61 atttattcac tctcctggct ggaatggccg cccccaaacg cgcccgctat tttctctcca
      121 ccgttatcat ggtcacgaac ggatgatact gccacggcac gctgccgcct ttgcggtaca
      181 tcttcgacag ggtgccgtcg agataaagca tctgccgcac gccaagcttt gactgcgcat
      241 agcaggcaaa atcgtaaaag ttggtctcgc gatcgctgag cataaacacc acccggccct
      301 gcttatcgat accaaccccg ttgcgcagct tgcgcgaact ggcggagggc ttcagtcgcc
      361 agttgatgag gccattttca atcagcatcg ggccggactg caccgcatag cggatggcgg
      421 gagagggttt gtacgccggc agcggcacga ttttcgcccg gccgttttcc accaggaata
      481 cgccgccggg gcggataaag aagttcccgc cgccggcgga gcggttgacc ggcgtcagct
      541 gtttgccgtc ttcgatatac agccccagcg gcgcgtaggc cttatcgtaa atgccgccgt
      601 tcatcgccat ctgcacccgg ccgtcgccat cgatacccgc cagcagcgaa cgcagggaac
      661 cccaggcttt gccgtgccga tcctgccagt acatcgcaat gcgctctttc tgcgcatcca
      721 cccgatagct ctgcagggtc agcgtcgggt cggtgagggt acaggcggcg ctggcgacgg
      781 cggccagggg cagcagcgcc agagggagca ggcaacgtaa taacatgggc agtcctttct
      841 atagtctgcg ccgagtatac ccgcctcgct gccttatgcc gcccaaatct cacagtgttt
      901 ttgcaccagc ccggaaagcg aaccgccgtt ggcgataaag tcgcgcatct gctgcgcctc
      961 gctttgcggc gatttcgcct ggcgaaccac cgcctccagc gctgccgtcg ccttcagctg
     1021 atgggcgaaa ggggccagcc ggtcggcgag ctgcaggatc tcctggcgga tgctgcgctg
     1081 ctccccgctg cgcacgtcgg tgagggtccc gtccagcccg tagcggcagg cctgatagcg
     1141 gttaaagggg tacagcagat aatcatcagg ctggtgctta aaaggacgct ccgtcaacag
     1201 ccagcaggcc agggtctgaa taaagccggc gatgtggatc gcctgggcaa gggtcagggg
     1261 ggtgtccatc acccgcacct cgacggtacc aaactgcggg ctgggacgaa tatcccagtg
     1321 cagatccttc atgctatcga tcatgctggt gtagctcagc tggcggaaca ggcgacgaaa
     1381 cccctgccag tcggccaccc acggcatcgg accgttgtcg ggataggagg agaagcggtt
     1441 caggcgcgaa caggcgaagc ggctgtcggt gctgtcgaac cacggtgagg cggcgttaag
     1501 ggcgatgaag tgcggtacaa agcgcgacag gccatgcagc agatagatgg cgtcgtcgcc
     1561 gctctggcag ccgacgtgca catgctggcc aaagaccgtc gcctgctgtg ccagatagcc
     1621 aaaatgctcg acggttttga catagcgcgg gttatcgctg atttgctggc gctgccaggc
     1681 atgaaaggga tgggaacccc cgccgcagat ctgcagatga tggcgcagcg ccgcgcgctg
     1741 gaccgcctgc tggatggcgc tgagctggat ttgcgcatgg ctgatgtcgc ggcacacccc
     1801 ggtggcgatc tccagcatgc tctcggtgat gtcgtgtttg gcttctccca cggtcagctc
     1861 atgctggacg tcggcaatca gcgtcgaggc atcctggctg aggtcgtaac ccggcgggtt
     1921 caccacctgc agctccagtt cgataccgag ggtaaaagga tcggagcggt gaaaatcggc
     1981 taaaggcata gtctctcccg gtcgatgtgt tccctgaagt atagaaggga ttgatcccga
     2041 cggagaatac gcggcggcgg tggacggggg atgaataaaa agtgacgcag agtgattatt
     2101 tgaaatttgc catatagcgg ctggtgcaat tctgcaacat atttcccgct ggcggtgcgg
     2161 cggaataaga tgcaattatt ttcccataat tatcatggtg ataatataag tcattcttat
     2221 tttaccggtc ggttttggcc tattttcgtc cgcgtaagag ctggtacgca ttttgcctgt
     2281 taatccgtga cactttattt aacagggatc atgtgatgca caaaaagacg ttattttcgc
     2341 gtgccggtcg tctcgccgtg gcggtgagcg gcgcactggc cctggcgggc gccgccttac
     2401 ctgccaacgc ctccacgctg cggatcgcca tgaccgccgc ggatattccg ttaacccttg
     2461 gccagccgga tcaaggctat gagggcaacc gctttaccgg catcccgctg tacgacgcgc
     2521 tggtggagtg ggatctgtcg caaggtgaaa agcccagcgg tctggtgccg gggctggcga
     2581 ccgagtggca catcgatccg cagaacacca gccgctggat tttcaccctg cgtccggggg
     2641 tgaagtttca tgatgggacg gaggtgaacg ccgacgccat cgtctggaac gtggataagg
     2701 tgctgaataa agcggcgccg cagtacgccc cgggtcagat cggcaacacc ctgtcgcgca
     2761 tgccgaccct caccggcgca gagaaaattg acgaccacac cgtggcgctg accacctcgg
     2821 aaccggatgc gctgctgccg tacaacatca ccaacctgtt tatcgtctcg ccgacggcgt
     2881 ggcagaagca gtatgacgcg gtgccggcca gcgtgagaga tagcgccgcg cgcagcaaac
     2941 aggcgtggac cgccttcgcc gcccacgcgg tgggtagcgg cccgttcaaa ctggagaagc
     3001 tggtgccgcg tcagcagctg atcctcgata aaaacccgga ctactggaac aaagatcgca
     3061 ttccccgggt ggataaagtg gtgctgatcc ccttgccgga ggccaacgcc cgtaccgcgg
     3121 cgctgttgtc gaagcaggtg gactggattg aagccccggc gccggacgcc atcgatcaga
     3181 tcaagtctca gggttttcac ctctacgcca acacccagcc gcatctgtgg ccctggcagt
     3241 tctcctttga aaaaggatcg ccgtggcagg acatccgcgt gcgcaaagcg gccaacttgt
     3301 gtctgaaccg tgccgagctg aagagctacc tcggcggcta catgaccgag gccactgggg
     3361 tctacgaggc tgacagcccg tggcatggca aacccacctt ccagataaaa tatgacccgg
     3421 acgccgcccg ccagctaatg accgaggccg gatacaatgc cagcaaaccg ctgcacgtca
     3481 cggtggctac ctcggcctcc ggctccgggc agatgcagcc cttgccgatg aacgagtata
     3541 tccagcagag cctgaagagc tgcttcttca atgtggatat caaagtggtg gaatggaaca
     3601 ccctgtttac caactggcgc ctgggcgccc gggacccctc ggcgaaaggg atcgatgcga
     3661 tcaacgtcag cgccgcggtt aacgatcctt actttggcct gatccgcttc tcgacggcca
     3721 aagcgtttcc gccggtggcg accaactggg gttacttttc cacgccggag acggagaagc
     3781 tggccgccgc cgtcaagcac gcctttaccc cggcggagat gaacaaagcg gccggtgagt
     3841 tgcacgccgc gctggtggat caggtgccat tcctgtttgt cgcccacgac gtcgggccgc
     3901 gtgcgatttc ccctgcggtg accggggtgg tgcaaccgca gagctggttt atcgacctca
     3961 gcctggtgag caaaaaagag taatcgcgaa aggggcgacc gatgatcaat acgctgctat
     4021 accgcattct gctggctatc cccaccatgc tgggggtggc ggtgatctgt ttcatgctgg
     4081 tgcagatcgc cccgggcgac ccgctggtgt cggtgatgcc gccggacgcc tcggaggcgc
     4141 tgcgtcagac attaatgcag gcttacggct ttgataagcc gctgcccctg cagtttatcc
     4201 actggctgtg gcgggcgctg cacggcgacc ttggtatgtc ggtcgccacc ggccgcccgg
     4261 tgatagacga agtgatgacc gcggtggctt actctctgcg cctggcgctg ctggcgacgg
     4321 cgatcggttt tgtcctcggc agcctgttcg gctttgtcgc cggctatttt cgcaacagcg
     4381 tcattgaccg tctggcctcg gtattgtcgg tgtttggcgt cagcgtgccg cactactggc
     4441 tggggatgct gctggtgatc ctctgcagcg tcaagttcgc gctgctgccg gccaccggcg
     4501 gcgggccgat aggcgaggtg ggttggcaat gggactgggc gcacctgcag ttcatgctgc
     4561 tgccggccct caccctgtcg gtgatcccga ccgggattat cgcccgcacc gtgcgctcgc
     4621 aggtggccga tatcctcagc caggagttta tcgttggcct gcgcgcccgc gggctcaacg
     4681 agtcgcgcat ctttgtccat gtcatgaaaa atgccgcgcc caccgcgctg gcggtgatgg
     4741 ggctgcaggt gggctatctg atgggcggct cgatccttgt ggagaccgtt ttctcctggc
     4801 cgggaaccgg attgctgctc aataccgcca tttttcagcg cgacctgccc ctgctgcagg
     4861 gcaccatctg ggtgctggcg ctgtttttcc tgctgctcaa cctgctggtg gatattttgc
     4921 agaccaccct cgacccgcgt attaagagga gctgaccatg gtggatacgg caacgacgaa
     4981 agcggtcccg gccgcagcgg cccacgctac ccggcaaggt tactggcgcg gcgtgctgcg
     5041 ccgcctactg cgcgaccccg gcggggtggt ggtgggcgtg gtgatcctcc tgctgctggc
     5101 cctggcgctg ctcggtccgt ggcttatcgt caaagatccc tatcaaacgt cgatgttcct
     5161 gcgcctgaaa cccatcggca gcgatggctt cccgctgggc tcggatgagc tgggacgcga
     5221 catgctgtcg cggctgatcc ttggcacccg actgtcgctg tttatgggaa ttgtcccggt
     5281 ggtgtttgcc ttttttatcg gcggggccat cgggattatc gccggctaca cgggcggcaa
     5341 aaccaatacc gtgattatgc gcactgtgga tgtgttctat gcctttccct ccgtcctgct
     5401 ggcgattgcg ctgtcgggag cgctgggggc gggcatcggc aatgcgctgc tgtcgctgac
     5461 cctcgtgttt gtcccgcagg tggcgcggat tgccgagagc gtgaccgccc aggtgcggca
     5521 tatggactat atcgacgccg cccgggccac tggcgccagc gcgttgacca ttatccgcgt
     5581 ccaggtgttg ggcaacgtgc tcgggccgat cttcgtcttc tccaccgggc tgatttcggt
     5641 gtgcatgatc ctcgcctccg gcctgtcgtt tctcggtctc ggcgtgcggc cgccggagcc
     5701 tgagtggggg ctgatgctca atacgctgcg cacggcaatc tatacccagc cgtgggtggc
     5761 cgccctgccg ggcctgatga tttttatcac ctccatttcg ttcaatatcc tcgcggaccg
     5821 cctgcgtgcg gcaatggcga ttaaggagta agcgatgcaa ccctttatca atatcgacct
     5881 tggcgggccg gcgcagccgc tgctgaaggt caatcacctg ctgaagtact ttcgcggcgg
     5941 caaaggccgg gaggtggtgc aggcggtgga cgatatcagc ttcacggtga tgaaaggcga
     6001 aaccctgggg gtggtcggcg agtcgggctg cggcaaatcg accaccgccc ggctgctgat
     6061 gcagttgctc acccaggaca gcggcgaact gatcttcgac ggacaggggg tcggctcgtc
     6121 gcgtctgccg ctgaaagcct accgtcgcca ggtgcagatg gtctttcagg atagctatgc
     6181 ttcgctcaat ccgcgcctga ccatggagga gagcatcgcc ttcggccagc gcgtccacgg
     6241 cgtcagcgcc agggaggcca gcgaatatgc ccgctatctg ctggcgcacg ttggccttga
     6301 gccggcgcgc tttgccgacc gctacccgca tgcgctctcc ggcggccagc ggcagcgggt
     6361 gaatatcgcc cgcgcgctgg cgatgaagcc gcggctggtg atcctcgatg aggcagtgtc
     6421 ggcgctggat aaatcggtgg aagcccaggt gctgcacctg ctgcaggacc tgaaacggac
     6481 tctcgagctg acctacgtgt tcatcagcca cgatctgcac gtggttcgct ggctgtcgga
     6541 tcggatcctc gtcatgtacc ttggcgaagt ggtggagatt ggcccggcgg agcagctgtt
     6601 taccgcctcc gcccacccct atacccgggc gctgttaagc tcgatgccct ccatggaccc
     6661 ggaaaaccgc accctgacct cgccgctaag cggcgatccc cccagcccca tcgcgccgcc
     6721 gagcggctgc cgctttcaca cccgctgccc gcatgcgaag gcggtctgcg ccgaggtgaa
     6781 accgcggctg gaggcggtcg gggaggggca ccagagcgcc tgcctgatgg cgcagccggc
     6841 ctcgccctgg catcaaagca tcccccttaa ggaggtcagc catgtcggat aacgcctttg
     6901 tccatgtgcg cgacctgcgg gtcgtctttc gccgcgacgg acagaccatc aacgcggtca
     6961 acggggtctc ctttgaggta caaaaggggg aggtgatggc gctgattggc gagtcgggtt
     7021 ccggcaagag cgtgaccctg cgggcgctga tgcgcctgca cccgccaggg agcagtgagc
     7081 tctccggcac cctgcaggtg ggggacgacg aggtgctgac gatgagcgcc agccagctgc
     7141 ggcgctatcg cggcggacgc tgcgcgatga tttttcagga gccgctgctg gccttcgatc
     7201 cggtctacac cgtcgggcag cagattattg aagggttgcg ccgccacgaa gggctgtcgc
     7261 gccaggcggc ccgggacagg gcgctggagg ccctgcgcca ggtgcgcatt cccagccccg
     7321 agcggcggct ggatgcttac ccgcacgaga tgtccggcgg aatgcgccag cgggcgatga
     7381 tcgcccttgc gctctcctgt aacccgcagc tgctgctggc ggatgagccg accaccgccc
     7441 tcgacgccac ggtgcaaatc cagatcctga tcctgctgcg cgagcagcaa aagcagcgcg
     7501 ggctgtcgat cgtgtttgtg acccacgata tcggcgccgc ggtggagatc gccgaccggg
     7561 tggcggtgat gtacgccggg cggatcgtcg aggaagcgtc gatgagcgag atcctgcgcc
     7621 acccgcgcca tccttatacc caggtgctgc tgggcagtcg gccgaaagag gggcttaaga
     7681 aaggtgatgc cctgcactgc attcccggcg cgccgccgga tctggcgcgc ttgccgcccg
     7741 gctgcgcatt tgccgagcgc tgcccgcacc gtcgcccggt ctgtgagcag agtctgccgc
     7801 cgacggaatc cgtcgcggaa cgccacgcgg tgagttgcca ccgctggcgc gagctggcga
     7861 ttgacgctaa cgcccaggcc ctggccgtct gatcggcggc aaaaaaccat acaggatgaa
     7921 gaccaggcgt tgatagcgca tttccaccgc cggttacgct ggcggcgacc ttgatgtcag
     7981 gagactacca tgagcagtga tgtacttttt acccccgcga ccgacaccac cggctggcgc
     8041 atcaatggcg atcgcctgtg ggcgtcgtta atggatctcg cgcagatcgg cgccacgccg
     8101 aagggcggct gccgccgtct gaccctcacc gacctcgacc gccaggggcg ggacaaggta
     8161 ataggctggg cgcgggaggc cgggatgagc gtcaccatcg ataaaatcgg caatgtgttt
     8221 atgcgccggg aaggccgcaa ccccggcctg ccgcccatcg tctccggcag ccatattgac
     8281 acccagccca ccggcggcaa atttgacggc aactacggcg tgctggcggc gctggaggtg
     8341 gtgcgcacgc tcaacgatct gcagattgac accgacgcgc cgattgaggt ggtgttctgg
     8401 accaacgaag aggggtcgcg ctttgtgccg gtgatgatgg gctccggggt ctttgccggg
     8461 gtcttcccgc tggaggagac ctgggccgtc acggataaag agggcgtcag cgtcggcgag
     8521 gcgctggagc agatcggcta tatcggcgag cagaccccgg gggaccatcc catcggcgcc
     8581 tattttgagg cgcacattga gcaggggccg attctcgaag atgaagcgaa aaccatcggt
     8641 atcgtgcagg gcgtgctggg gatccgctgg tacgactgcg tggtcaccgg ccaggcctcg
     8701 cacgccggac ccacgccgat gcgcctgcgt caggatgccc tgcaggtggc gacccgcatt
     8761 atgcaggaag tggtggccat tgccgggcgc agcgaagagg gacgcggcac ggtcggtagc
     8821 gtgcaggtgt ggcccaacag ccgcaacgtg gtgcccgggg aggtcacctt ctccattgat
     8881 atgcgcaatc tcagcgatgc gctggtggac gagatggatc gccagctgcg ggcgtttatc
     8941 gcggaggttg agcgggagag cggtctgcag gtggccctga agcaggtaag ccactatccg
     9001 gcggcgccgt tcgatgccga atgccagcag gccattgccg atgccgcgca gcggctgggc
     9061 tatccggcgc ggccgatcgt ctccggcgcc gggcatgacg cagtgtatat gagctacctg
     9121 gcgcccaccg ggatgatttt tatcccctgc aaggacggga tcagccataa cgaaattgag
     9181 tatgcctcgc cggagcacgt cgccgccggg gccaacgtgc tgctgcaggt gatgcttcag
     9241 tacgcgcggc cggtgtagac ggtcgtcctc cccggcggcg cgtcctccag ccgtttatca
     9301 tgctgtcacc cgggtaaggc ggttacaccg acgcgacccg ggacggcgga tgacggggcg
     9361 gtcaatcacg gctcccgctc cctgcgccaa cccgctggcg ggcaaatttg cgcgagccca
     9421 ccacgcacag caccacgccc agcgtgaccg ccagcatcaa ggggctgacg gtttcgtgca
     9481 acagcgccgc cgacaggcca agtccgaaga acggctgcaa tagctggagc tggccgacgg
     9541 cggcgatccc gcccgcggca agccctttgt accagaagat gaaaccgatc agcatgctga
     9601 acagcgacac atagccgagc gccgcccagg aggaggccga gatggcatgc caggacgcgg
     9661 gctgaaccag cagcgaggcg gggagcatca acggcagggc aatcaccagc gcccagcaga
     9721 tcacctgcca gccgccgagc tcccgcgtca gcttcgcccc ttccgcatag ccgaggccgc
     9781 agacgataac cgccgccagc atcagcagat cgccgctgag cgaggccgcc gcattttgcg
     9841 tcagggcgaa tcccaccacc agcaggctgc ccagcagcga aaagatccag aaagcccgcc
     9901 gcgggcgttc gccgccgcgc agtacgccaa agagggcggt catcagcggc agcaggccaa
     9961 taaacacaat cgaatgcgcc gaggtgatgc gctgcagggc cagcgccgtg agtagcggaa
    10021 agccgaccac caccccggat gagacgatga tcagggacac aagctgcgcc aggcgcgggc
    10081 gtttctggcg aaagcccacc agcagcgcga tggcaagcag cccggcgata gaggcgcgca
    10141 gaaaggtcaa taacaacgga tccatatcct gcaccgccag ccgagtcgcg ggcagcgaac
    10201 cgctgaaaat gatcaccccc agtaaaccat gaacccatcc ggaccaggga ccgggcgccg
    10261 atttatccat tgctgaatct accacgttaa cctcgcgtta tctgattggg ctgcgcccgc
    10321 ttgagcatcg tcagcggcga tggtaatgtg gccaatctaa tacaatcaaa ttattgtcat
    10381 agatacattt cacgatgaaa gcccgatata aagccgttgt cgaccgatat gcgcaggcga
    10441 tccgcagcgg gcagctgccc gccggcagcc gtctgccgac gcaccgtacc cttgccgccg
    10501 gagaacgcct ctcgctggcc accgccacgc gcgtctaccg tgaactggaa gagatggggt
    10561 tggtcagcgg cgaaaccggc cgcggcacct tcgtgcgcga cctctcgctg ccgccggggc
    10621 atggggtcga tcagcaggtg gtggccgccg atgtcgtgga tctcaatttc aactaccctt
    10681 cgctgccgga tcagggcgat gcgctgcgcg aggcgctcag gcagctggcg atggcgggcg
    10741 atattgactc gcatctgcgc tatcagcctc acgccgggcg gctcgccgag cgggacatta
    10801 tcgcccgcca tttgacctgc cagcactttg cgccggacgc ggaaaatgtc ctgatcgtca
    10861 acggcgcaca gcacgggctg gcggtgaccg tcatggggct gttacgtccg ggggatgtgg
    10921 tggcggtgga tgcgctgacc tattcgggct tcaaggttct ggcggcgctg tatcatctgg
    10981 agctggcggc gatcccctgt cggccggagg gaccggacct gcaggcgctt cagacgctgt
    11041 gccagcagcg gcgggtgcgg gcggtgtata ccatgccgac gctgcacaat ccgctgggct
    11101 gggtgctcaa caccgggcag cgacaggcgc tggccgatct cgcgcgccag cacgatctgc
    11161 tgattattga agatgccgcc tacgcccggc tggtgagcca tccgccgccg ccggtggtca
    11221 gctatgcgcc ggaaagaacg gtctatgtca ccggattttc aaaaaatatc gccaccggac
    11281 tgcgcgtggg ggtggtgatt tctccgccgc gctatcgccc ggagattgag cgcgccatcc
    11341 gggccacgac gtggaatacg ccgacgctga taagttcgct gatctgcgcc tggattgaag
    11401 atggcacggt ggcgcgcttt gaaacgcaga aacgccagga tgcgcgacag cggcagcagg
    11461 tggcccgcga gctactttgc gggcttcccg tcgtcagtca tcccgattcg tacttcgtct
    11521 ggctgcctct gggtgaagag agccgggccg atcggctggc gaatgcgctg atggaacgcc
    11581 gcatttcggt atcgaccgcc gagccgttct gcgtctccgc cacgatcccg caggcgctgc
    11641 gcattgcgct cggatcggtg cctttcgaca gcctgcgccc ggcgctgctc agcgtgcgcg
    11701 acgccgttga gtatgaacaa taccgctaac ccatctgctc cagctggccg cgcaggacct
    11761 ccaccccctg ggcgatggcg tcagggttaa tggcgtggaa tcccatgcga aaatagttat
    11821 ccggcggcgc ggcgttaaga aaatggcgcg ccccgggctc aatcagcacg ccggcgtggg
    11881 cggcgcgcca ggtgagctgc tgggtgttga tctgcgccgg ggtctgcagc cagaaggcgt
    11941 tagcatgttc gctccccgcc agcgcgcgac acgacggcag atagcgctgc agggcggcgt
    12001 gcagacactc ccagcgctgg gcggagtcat agtggtagcg ccgcagatgg gtctcataat
    12061 ggccctgtgc aaggaagtgg gccatctggt actgaatgtt ggtcggcgga tggcgataga
    12121 ccagccggcg cagggcgcgc gcctcgtcga tcaggtccgg gtcggcgacc ataaacccca
    12181 accgcaaccc cggtgacagc gcttttgaca ggctgctgac atacaccacc cggccgctgc
    12241 gatcgctggc cttcagcgcc ggcagcgggt tgagggtaaa gttactctcc gagtcataat
    12301 catcttcaat aatgaccgcg tcatggcggg tggcatgctc cagcaactgg cgacgccgcg
    12361 cactgctcat ggtcaccccg gtgggcacct gatggcctgg tgtgacatag tagtaatcgc
    12421 agggacgctc attaagcacg atcccctctt catccaccgg gtgcggcacc acctcggcgt
    12481 cggcgagcag aaaggtgttg atggcttcgc ggaagcaggg gttttcgacg ccaatgcggg
    12541 tggtggaaga gagcagcagt cgggtcagca ggtagagcgc attttgcgaa ccaagggtga
    12601 tgagaatttc atccggcgcg gcgacaatgc cgcgcttggg cagcacccgg gtgcggatct
    12661 gctcgatcag catcggcaca tcctggtcaa tatggtcgat gacccacgcc gggtcgcgca
    12721 ccccgccgtg cagccagttc gccgccgagc gccaggtggc cagcgggaat tgccgggtat
    12781 cgggctggcc gtagataaac gggtagcggt agtgcatcca gcccgccggt ttgaggatcg
    12841 actcctgctg gctgggggtc atctgcagcc ggtcgcccca gcgtggtgca gccgcttcgc
    12901 gctgcggtgc actctcgacg gcggcaggcg aagcttcatg atagtcggga tgcaggtagt
    12961 aaccgctgcg tgggcggcta atcaggtagc cctcgttgac cagactttcg aacaccagcg
    13021 cggtggtgtt gcgcgagacg cgaagctggc tggccaactg gcgacaggag ggcagcgggg
    13081 tgtcggcggc aaagatcccg ctgagaatgg cattcaccag cgtctctcgc acctgttcct
    13141 gcaaaccgcg gccaggctga aaatcaacgt gtaacaggtg acggatcata cggggctcct
    13201 ctggcaataa cgcattgcga ccacccgtta cactcagcaa attccatacc accctgccgc
    13261 catctgacac agcgatgcgg cccatctggc tctatacgga attgaaaaac gctcccacat
    13321 taaaaacgta ttcagccgcc gtggaccgga acgtccccgg ccactatttt gcagaaacct
    13381 tttcgcgttc tggaatgaac ccgtctggcc aatcaatcag gggtgaaata atgaaaaaat
    13441 cattggcatc aatgtgtctg agcgctgtat taacggtcgc tttttcttat cacgccgtcg
    13501 cggccgatct tccggaaata gaaaaatccg gcaccttgaa agtggcgacg gaagatgatt
    13561 atgcgccgtt taactttatg aataatggcc aggccgatgg ctttaacaaa gatatgcttg
    13621 aggaattacg taagtacgcc aaattccatg tcgaccagag catattaccg tggaccggat
    13681 tattagcggc tgtctccacc gggcaatacg atatggcctt aaccggggcg gttattaccg
    13741 atgagcggct gaaggtcttt gatttcaccc caccgtgggc ctccgcgcag cactatttcg
    13801 tcaaacgcgc tggcgatacc tcgctaaata ccattgccga tctcagcggc aaaaaagtgg
    13861 gggtacaggc cgggagcgcg ctgctggcgc gtttgccgga gctgaaggcg atgctggaga
    13921 agaccggcgg caagctgggg ccggtggtgg agtatccctc ctatccggaa gcctacgccg
    13981 acctggcgaa caagcgactg gattacgtga ttaacgtggt gatctcggta aacgacctgg
    14041 cgaaagccaa accgaaggtc ttcgccaaag ggctggccgt ctccggaccg gggtatatgg
    14101 cgtggccgat cccgaaaaac tcgccgcagc tgctggccta tatgaccagg tttatgaacc
    14161 acatgaagga gaccggcaag ctcgccgagc tgcagaaaaa atggttcggc gaaacctatg
    14221 acaacctgcc gaccgaggcg atcaccagcc cggaacagtt tcacaaactc gccggactgt
    14281 aatattccgc ggcgggcgac cgccgcctgt tgttgagaag gagggcctca tggatgcaat
    14341 ttcctggcaa ttattaatcg aaggcgcatg gacgaccctc tggatttcgg ccatcgctat
    14401 cgccttcggg gtggtggctg gcctgctgat tgccctggta cggatgctgc gcctgccggt
    14461 gatcgaccag ctgctggtgg tttatatcag tctggcccgc gcgacgccgc tggtgacgct
    14521 ggtgctattt ctgttccttt ccctgcccac gatgggcatt aacctcgaca agaacgtggc
    14581 cgccatcgtg gcgctgacgc ttaacacgtc ggcgtttaac gctgagatct ggcgcaatgc
    14641 cttccgcacc tttccgcgcg aacagcggga ggcggcggag tcggtaggga tgcgccgctg
    14701 gacctacttc cgctacatca tgctgccgca gatgtggatc gagagcctgc cggcgctggt
    14761 caatgagatg tcctttttga tcaaaggtag ccccgcgatc gccgtgattg gcgtggtgga
    14821 tctcacccgg gtcaccaacc gtatatcctc ggtcacctac gagccgctgt cgccgatcct
    14881 cgccgccggc ctgctgtatg tggcgatcat cggctgtttg ttgaagctgc agggtatcgc
    14941 ggaacgtaaa gccaggcgtc tggcgcgcta ggaacgggag gcattatgaa tcagtggggc
    15001 gttatctggt cggttcgcga tagctttatt gccgggttat tcgcgaccct cgaactgttt
    15061 attaccgccg ccctggcggc gctgattatc ggtattgcgc tgtgttattt tagcgaatat
    15121 caaaagaaag tgattaaccg gattattatt ggtttcgtca gcctgatgcg cgcgatccct
    15181 tttttgatcc tcgcttatct tctgtattac ggactgccgc agctcggtat tagcatggag
    15241 ccgtggaccg ccggtttact gtccctgatt atttatcatg gcgcctattt ctttgaaata
    15301 ttgcgcagcc agcggcgggt attttccggc ggatatattg aagcggcgat tgctcagggc
    15361 ttttctcgct atgccatttt tcggcggatt attttaccca atatcgtctc ttccgcctta
    15421 ccgctgatcg gcaatcagct gattatttgc ctgaaggaca ccgctttcct gagcattatt
    15481 accgttcagg agatcaccgc cgccgccaac agcgtccagg cgacctattt tattccgttt
    15541 aacgccttta tcgtcgccat tgggctttac tgggcgatca gcattctgct ggagctgctg
    15601 attaagcgcc tgaccgcctg gggagccaaa agaggaatga gccatgcctg agtctattgc
    15661 cgtaagcgtc aaaaacgtct ccaaacagtt cgataatgtg gaagtcctgc gggatattaa
    15721 cctgacggtg gagaagggga ccgtggtgag tattctcggc tcgtccgggt cgggaaaatc
    15781 caccctgctg cgctgtatga actggctgga acagccggac cgcggtgaaa ttcatatcgg
    15841 cggtcagcgg ctggggattg atgaacagcg cggccgggcg atgtcgcacc gccagctggc
    15901 caaaatccgc gagcgggtgg ggatggtctt ccagagtttt aacctgtggc cgcacctgac
    15961 cgtgcagcag aacgtcagcg aggcgctgct gcgcgttaag ggcatgaaac gagacgaggc
    16021 gaaggccatg gccatgcagc agctggagaa agtcggcatg gcgcacaaag ccgatgtcta
    16081 cccgatcact ctctccggcg gacagaagca gcgggtggcg atcgcccgtt cgctggcgat
    16141 gtcgccggaa gttattctct tcgatgaacc gacgtcggcg ctggatccgg aactggttaa
    16201 tgaggtgttg ggggtaatga aagcccttgc cgccgagggc tacaccatgg tggtggtgac
    16261 ccacgagatg gattttgccc gccaggtgtc caacgaggtg gtgtttctgg agaagggact
    16321 gctgattgag aaggcgccgc cggagaagtt cttcactcag cctgactccg aacgcgtcag
    16381 gcagtttctg caaagcagcc gttagtccgg cctgcatccc ctgccggtat cgccggcagg
    16441 ggatgcgtcg ggtttcgctt aatcccggtc gatagcgaac ggctgccacg cctgacgcac
    16501 cggcatcacc tccacccggt taatgttcat atgcgccggc agcgtggcga tataaaacat
    16561 ctgctcggcg atatcgcgcg cgctcagcgg cgtggtgccg cgatagagct tatccgatgc
    16621 cgcctggtcg cctttggttc gcaccagggt aaattcggtc tcggcaatcc ccggcgccag
    16681 atcggtgacc cgcacgccgg tgccaagcag atcgcagcgc aggttatagc tgaactgctt
    16741 cacaaacgcc ttgctggcgc catacacatg gctgccggga tagggccact gcccggcgat
    16801 cgagccgata ttgataatac tcgccccggc gccatggcgg atcagcgtcg gcagcagggc
    16861 gtgagtgact gtcaccagac cggtgacgtt ggtgtcgatc atcgttttcc agtcctccag
    16921 cgccacctcc tgcgccggca gcggcgacag cgccagcccg gcgttgttga tcagggtggt
    16981 gatatcggcg aaatcggcgg gcagcgacgc cacggcggca gccaccgcct cgctgtcacg
    17041 aacgtcaagc tcaataatat gcaccggcac ctgggcggcg agtctgtcct gcaaggcctt
    17101 cagtcgcgga tagcggcgcc cgctcagcac cagcgaccag cccgcgtcgg caaacacctg
    17161 cgctgccgct tcgccaaaac ccgaggtcgc cccggtgata aacactacgt tgctcgttgc
    17221 catctcattc tcctgtgcgg ttgtcccttc actataaaaa cgccggacga caaggcacag
    17281 ggccagacga cgggcgggga tgtgacaccg tgtcacatcg ttatgcgcgc actggctcta
    17341 tccgatttgc gcggccaagg ccaatgatga agccaaccac tacgacatga gagagggcag
    17401 ggtatgaagc tggcaattca caacgaggtc gcggtaagca atgacgaagt tcggcaatta
    17461 gatcgtgcct atgtgtttca ttcatggtcg atgcagggca atctcaaccc gctggtcatc
    17521 gccggggcgc aaggctgcga gctatgggat tatgagggga acacctggct cgatttcagc
    17581 agccagctgg tgaacgtcaa cattggctat cagcaccccc gggtattggc ggccatgaag
    17641 tcgcagctgg agaccttggt caccatcgcg cccgccaccg ccaaccttgc ccgcggcgaa
    17701 gcggcgaagc gcatcgtcga cctggcgccg gcgggcttca gtaaggtgtt cttcactaac
    17761 gccggggcgg atgccaatga gaacgcgatt cgcatggcgc ggctctatac cgggcgcgac
    17821 aaggtgctgt cggcctatcg ttcctatcat ggcaacaccg gcagcgcgat cgccgctacc
    17881 ggcgactggc gccgggtgcc gaatgagttc tcccgcggcc acgttcactt cttcaacccc
    17941 tacctttacc gcagcgagtt caacgccgcg actgaggaag aggagtgcca gcgcgcgctg
    18001 gcgcatctgc ggcggatcat tgaatgcgag gggccgacgg ccatcgcggc gatcctgctg
    18061 gagtccatcc ccggcaccgc cgggatcctc gtgccgccgg caggctatat gcagggcgtg
    18121 cgcgccctgg ccgacgagtt tggcatcgtg ctgatcctcg atgaggtgat ggccggcttt
    18181 ggccgcaccg gcagctggtt cgccttcgag caggatggcg tggtgccgga tctggtgacc
    18241 tttgccaagg gggttaacgc cggctacgtg ccggcgggcg gggtgctgat tagcgagccg
    18301 attgcccgct actttgacga ccacttcttt gccggggggc tgacctactc cggccatccg
    18361 ctggcgatgg cggcgattgt cgccaccatc gacgcgatga aagaggagaa ggtggttgag
    18421 aacgcggcct ctatcggcaa cgaggtgctg cgccccggtc tcgaggcgct ggcggagaag
    18481 cacgccatta tcggcgaggt gcgcggtcgc ggcctgttcc aggcgctgga gctggtgagc
    18541 agccgtgaac aaaaaacgcc actgaccgcc gccgacatgg ccgcgatcaa aggggccctg
    18601 accgaagccg ggctgctggc ctttgtggtg gagaaccgca ttcacgtggt gccgccgtgc
    18661 accattactg ctgagcaggt ggcgcaaggg ctggcgattt ttgatgcggt cttcgctcgc
    18721 tttgccagcc tggcgaagta ggggaggggg ccggatactg ttgcccggcg gcgcgcttgc
    18781 acgggcgtac ggggtgtgag cctgtgagtg gatcctgtag gccgggtaag gcgcagccgc
    18841 cacccggcga aatcaaggat accgtgcctg atatcttgcc cggcggcgct gcgcttgccg
    18901 ggcctacggg gtgtgagcct gtgaatggat cctgtaggcc gggtaaggcg cagccgccac
    18961 ccggcaacac cacgggtacc gtgcctgatt attgcccggc ggcgctgctg gactgcaccc
    19021 caaaagttgg acacccaact gagtaaggtg caattttatg gctaaaccaa agtattcccc
    19081 tgaaacaaaa ctggctgtgg ttaatcatta tttgtccggt aaagacggag aacagagtac
    19141 agccgacctt tttggtattg aaagaacatc tgtccgtcgc tgggtcaggg catggcagtt
    19201 ccacggtgca gaaggcctga ctgcaaaaaa taatcattat tccgatgaat ttaaactcgt
    19261 ggtcgtccgg gcggttatca gtgaccgcct gacgatgcgt gaagcggctg cccggtttaa
    19321 tctctccgca gaaatacttg tccggcgctg gctcgacgtg tacaacgatg ccggagcgga
    19381 aggtctttta aacatgcaat gcggacggcc cggacaaatg acaaagccaa aaaatatccc
    19441 accactgaca gataaagagc tggaaaaact ctcccccgaa gagctcaggg ccgaactgcg
    19501 ttatctgcgg gcagagaatg cctatctaaa aaagttgaaa gccttggttc agagcgaaaa
    19561 aaatggcaaa aagccctgat aatcagtgaa ctaaggcatg aacacgctct gcgggacctt
    19621 ctgcgggcgg ccggtatgtc ccgtagcacg tggtattaca atatgaatgc actgaagcaa
    19681 ggggacaggt atgcgggtct taaagagaac atcaggaaga tataccacta tcacaaaggt
    19741 cgttatggct accgcaggat cacgctcgca ctgagaaaac aggggctgcg gataaaccat
    19801 aaaacagtgc agcggctgat ggcagaactg tcactccggt ctgtgataag ggcgaaaaaa
    19861 tatcgtgcct ggaaagggag aacgggcgag gccgcgccca atatcctgag ccggaacttc
    19921 ggtgcgtcaa aagccaacga aaaatgggta acagatgtga cagagttccc ggtacaggga
    19981 aaaaagcttt acctgtcgtc agttctcgat ctgtttaacc gggaggttat cgcctacagc
    20041 ctgtcggaaa ggccggtgat ggagatggtg aatacgatgc tggacggtgc gttcccgaag
    20101 ctcagaccgg gagatgctcc gctgctgcac tcggatcagg gctggcatta caggatgagg
    20161 agctatcagg aacgtttaaa ggcgcatggg atgacgcaga gtatgtcgcg taaaggaaac
    20221 tgcctggata atgcagtgat ggaaaacttc ttcgggaccc tgaaatcgga gtgtttttat
    20281 ctgagggagt tcaggagtgt cagtgcgcta agaaaagccg tagaggacta tatccattac
    20341 tacaacaacg agcggataag cctgaaatta aaaggcctga gtccggtaga gtaccgaacc
    20401 caggctctga gagccgctta atatgaacca tccaacttta tggggtcagt cca
//
