LOCUS       NZ_CP099726             3555 bp    DNA     circular CON 10-JUL-2023
DEFINITION  Escherichia coli strain EC21GDE043 plasmid pGDE043-5, complete
            sequence.
ACCESSION   NZ_CP099726
VERSION     NZ_CP099726.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29354608
            Assembly: GCF_024125695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Huang,Y., Liu,C., Tang,B. and Guan,C.
  TITLE     Complete genome sequence of mcr-1-harboring E. coli isolated from
            Guangdong
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Huang,Y., Liu,C., Tang,B. and Guan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Mar-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/10/2023 04:28:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,357
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 1 of 285
            Pseudo Genes (frameshifted)       :: 89 of 285
            Pseudo Genes (incomplete)         :: 191 of 285
            Pseudo Genes (internal stop)      :: 64 of 285
            Pseudo Genes (multiple problems)  :: 56 of 285
            Pseudo Genes (short protein)      :: 5 of 285
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099726.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21GDE043"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="pGDE043-5"
                     /geo_loc_name="China:Guangdong"
                     /collection_date="2021-03"
     gene            complement(249..974)
                     /locus_tag="NH569_RS26705"
                     /old_locus_tag="NH569_26705"
     CDS             complement(249..974)
                     /locus_tag="NH569_RS26705"
                     /old_locus_tag="NH569_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_052895205.1"
                     /translation="MASMETEQQPQQTTEQIEVKTKRVQCFIDGYNLYHAIEETGNKAL
                     HWVDLKSLCKRYLPKNASIDNVFWFSAKPSHLSRAINENYQNYTNALTECGVDVIGGRF
                     KKKNVTCEADNGCGGVFHKHEEKESDVNLAISLVSGACNDEYDIAIIITADSDLCPPIR
                     YVRERFPNKELWLVAPPGRIRRATDLCSLSSRSMEIKNDALRACCMRDEFYKDGVLVAR
                     RPQEWRAVANTRQRRARSR"
                     /name="NYN domain-containing protein"
     gene            1295..1909
                     /locus_tag="NH569_RS26710"
                     /old_locus_tag="NH569_26710"
     CDS             1295..1909
                     /locus_tag="NH569_RS26710"
                     /old_locus_tag="NH569_26710"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_052895292.1"
                     /translation="MSTKNAQKEQKKESLITTHWGDDATIHGWTAIPNSLLMLQGDLGI
                     GSTEMCILLNILMHQWPESGESISFPSIGTIASRIGVSKRTIQRGVSNLEALGILTRYQ
                     STRNDPLTNGANIFDSTPLKEYLNKKAKGITISNSNKKKERKKTGITPNIKLPRICPKC
                     LKTKATSHEEIVKFFGVRKTMAGEIINSYCKECRASEKDLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2197..2520)
                     /gene="mobA"
                     /locus_tag="NH569_RS26715"
                     /old_locus_tag="NH569_26715"
     CDS             complement(2197..2520)
                     /gene="mobA"
                     /locus_tag="NH569_RS26715"
                     /old_locus_tag="NH569_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2804..2998
                     /locus_tag="NH569_RS26720"
                     /old_locus_tag="NH569_26720"
     CDS             2804..2998
                     /locus_tag="NH569_RS26720"
                     /old_locus_tag="NH569_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_052895291.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLRLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2530..2769)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          3034..3555
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.437"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(2201..2520)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.86e-62"
ORIGIN
        1 aaagacacga cttaacgcca ctggcagcag ccattggtaa ctgatttaga ggagttgagt
       61 cttgaagtta tgcacctgat aaggctaaac tgaaagaaca agttttggcg actgtgctcc
      121 tcaaagccag ttacctcggt tcaaagagcg ccaactctat gaaccttcga aaaaccgccc
      181 gccaaggcgg ttttttcgtt ttcaacctag ttagattaat ctccttgcgc tagctgcgtc
      241 aacaaagcct aacgacttct agcccttctt tgcctggtat tggcaacagc tcgccattcc
      301 tgaggccttc tggctacaag tactccatct ttgtagaact catcacgcat acagcaggct
      361 ctcaaagcat cattcttgat ctccatactt ctcgatgaca gagagcagag atctgtcgct
      421 cgcctgattc tccccggagg agctacaagc caaagctctt tattagggaa tcgctctctt
      481 acgtatctaa taggggggca taagtcagaa tccgcagtga taataatggc tatatcatac
      541 tcatcattac acgcaccaga cactagactt atagccaaat taacatcgga ctctttttct
      601 tcatgcttat ggaaaactcc accacatcca ttatcagctt cgcacgttac attctttttt
      661 ttaaaacgtc caccgataac atcaacacca cattcggtta aagcgttggt gtaattttga
      721 taattttcat ttatggctct tgataaatgt gatggcttag cggaaaacca aaaaacatta
      781 tcaatagatg catttttagg aaggtatctc ttgcataaag atttaagatc aacccaatga
      841 agagctttat taccagtctc ttcaattgca tgatataaat tatacccatc aataaagcac
      901 tgcactcgct ttgtttttac ttcgatttgc tcagtagttt gttgtggttg ctgctctgtt
      961 tccatacttg ccatttttga cctctagata gcaaaaaggc ctgcatatgc aggccttttt
     1021 gcgtctctta accgcagtta acagaccact gtatggacgc atccaataca ggaagaattt
     1081 tgtacaatgc gcttccaagc ctcgacttgg atagccacta agagcataac tcttaggtag
     1141 tggactcaac ttaagttcta tataaaaaaa gagcaaatgg atgacattta tgtcatccta
     1201 aaagatacat gttactacct aactactctt atattaagag tagttaggta gtaactgttc
     1261 acattttgat ttttttttcg cgagaaaagc atctatgagc acaaaaaacg cacagaaaga
     1321 acaaaaaaaa gagtcattaa tcactactca ctggggagat gatgcaacca tacatgggtg
     1381 gacggctata ccaaatagcc tccttatgct gcaaggtgat cttggtattg ggagtactga
     1441 gatgtgtatt ctgctgaata tattgatgca tcaatggcct gagtcagggg agtcaatatc
     1501 ttttccgtca attggtacca ttgcatcaag aataggtgta tctaaaagaa caattcaacg
     1561 cggggttagt aacctagagg cattggggat attaactaga taccaatcaa caaggaatga
     1621 ccctctaacc aatggcgcta atatattcga ctcaactcct ctaaaagaat atttaaacaa
     1681 aaaagctaaa ggcataacaa ttagcaacag caacaaaaag aaagaaagaa agaaaaccgg
     1741 aattacaccc aacataaaat taccacgcat ttgcccaaaa tgtttgaaaa ctaaagcaac
     1801 cagccacgaa gaaattgtta aattttttgg cgtgcgaaaa acaatggctg gggaaataat
     1861 aaattcatat tgcaaagaat gccgagcttc tgaaaaagat ctattttaaa cagttgacca
     1921 ttttttatat ggccaactgc cccccactca agtaaatggg actgtttcta acgctcccat
     1981 ttaccataac gtctcattac gcgcaccgca gaaacgcgct gaacgcgatt ctgccgcaaa
     2041 caccgcaaaa atagatgttt tccccggggt aaaccggaaa aatgcggcag gatcgctttc
     2101 agctcactgc atagctatgt atgaaagtga atggcgatcg gtttgtgggc ttacggcgtt
     2161 cataccgtct gttttcgaca gtttccctcc gggaagctaa tctgccataa gcctggataa
     2221 cagggcccgg tgatactccg taatagctat cagcacctcc gcatactctc tgtccccgac
     2281 acgcttgccg tcgataaaga gttttttctg taacgccccc agcctcataa cttctttcag
     2341 tacccgatca tcagtcagtg agttaacttt cttaccgaga gctgccgccc gtaaaaaacc
     2401 ggaaacggtc ttgccgcatt cagcagccct ttttctgatt tcctggtctt cttctgccgt
     2461 cagacgaaca gggcgactga tagcccgcct gcgcgtatta cttccacttc gttttgtcat
     2521 aaaacatggc tccgtatctg acatgggtgt cggggcaaag ccctgaccag ggtaattgta
     2581 atagcgtgca tgtatgcgcg gtataacaat tgcacatcct gtcctgtcag caagctcgaa
     2641 ttaccgacaa aagcacggca gttttttggc agtgacagtg ttgtctgaca gaaatgcccg
     2701 tcaaagagtc agacagcaga ataccggcgg taaaacagtc gaaaagtggt actcgatata
     2761 atcacacaac gcagatgaca gaaagaacgg agaaaacaga aagatgaaca agcagcagca
     2821 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgaggctgc tcgaaaaact
     2881 ggacgcactt gatgctgacg aacaggccgc catgtgtgag aagctgcacg aactcgcgga
     2941 agaacttcag aacagcatac agacacgctt tgaagcggaa aatcgcaccg ggatataacg
     3001 cacccggaca ggatgcagaa cggcatagcg gagcgtaagg agaaaatgcc acgtcagacg
     3061 ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt
     3121 gtcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgccggaaa
     3181 gaacatgtga gcaaaaagca aagcaccgga acaagccagc gccgatgccg tttttccata
     3241 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3301 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3361 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3421 tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3481 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3541 ttgagtccaa cccgg
//
