LOCUS       NZ_CP099725             4657 bp    DNA     circular CON 10-JUL-2023
DEFINITION  Escherichia coli strain EC21GDE043 plasmid pGDE043-4, complete
            sequence.
ACCESSION   NZ_CP099725
VERSION     NZ_CP099725.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29354608
            Assembly: GCF_024125695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Huang,Y., Liu,C., Tang,B. and Guan,C.
  TITLE     Complete genome sequence of mcr-1-harboring E. coli isolated from
            Guangdong
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Huang,Y., Liu,C., Tang,B. and Guan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Mar-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/10/2023 04:28:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,357
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 1 of 285
            Pseudo Genes (frameshifted)       :: 89 of 285
            Pseudo Genes (incomplete)         :: 191 of 285
            Pseudo Genes (internal stop)      :: 64 of 285
            Pseudo Genes (multiple problems)  :: 56 of 285
            Pseudo Genes (short protein)      :: 5 of 285
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099725.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21GDE043"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="pGDE043-4"
                     /geo_loc_name="China:Guangdong"
                     /collection_date="2021-03"
     gene            complement(join(4395..4657,1..82))
                     /locus_tag="NH569_RS26675"
                     /old_locus_tag="NH569_26675"
     CDS             complement(join(4395..4657,1..82))
                     /locus_tag="NH569_RS26675"
                     /old_locus_tag="NH569_26675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000764079.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     AGKLPSLSPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1308..1787)
                     /locus_tag="NH569_RS26680"
                     /old_locus_tag="NH569_26680"
     CDS             complement(1308..1787)
                     /locus_tag="NH569_RS26680"
                     /old_locus_tag="NH569_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001050742.1"
                     /translation="MNLLLPRDIVEAVLNDKKTKNARVAKCDGSEFFLELPSMNADFPA
                     GKIILKLGDSGFYNKRTKSLEGAYGLRHIWDKHRVEIGATSAEDIVIFLESILLAGAEV
                     LIDPKKGQNKAIVVESGTGMMILELKKPNGEDPYYSIITAYDRKSHPGTKLHTLI"
                     /name="hypothetical protein"
     gene            2047..2814
                     /locus_tag="NH569_RS26685"
                     /old_locus_tag="NH569_26685"
     CDS             2047..2814
                     /locus_tag="NH569_RS26685"
                     /old_locus_tag="NH569_26685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225568386.1"
                     /translation="MQPRELLMLSMYILVQAPVLTGLSKNQTESLTTNNKRGRRTVFYR
                     IKKHLQHDFYRIDSIKNEIHSPIKGFYMKDMKAVVVFTGKDLNIMRTEGGSGYWHARTD
                     RLNDADYLIAVRNRRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHT
                     PKAWKMLTDGQRYPVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKT
                     EKLSLNEAIERAKKDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(3013..3228)
                     /locus_tag="NH569_RS26690"
                     /old_locus_tag="NH569_26690"
     CDS             complement(3013..3228)
                     /locus_tag="NH569_RS26690"
                     /old_locus_tag="NH569_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3232..3717)
                     /locus_tag="NH569_RS26695"
                     /old_locus_tag="NH569_26695"
     CDS             complement(3232..3717)
                     /locus_tag="NH569_RS26695"
                     /old_locus_tag="NH569_26695"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140031164.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSAAMSQRTKGMLRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTTIREQKHTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3752..4186)
                     /locus_tag="NH569_RS26700"
                     /old_locus_tag="NH569_26700"
                     /pseudo=""
     CDS             complement(<3752..4186)
                     /locus_tag="NH569_RS26700"
                     /old_locus_tag="NH569_26700"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(163..349)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.77"
                     /coverage="87.0"
                     /evalue="2.7300000000000002e-42"
     Typing          329..808
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.938"
                     /coverage="93.0"
                     /evalue="3.51e-174"
     Typing          complement(2910..4405)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.984"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taccgtcgca gcgctccagc agacggcggt gctcatcttc ggtcacccac atcgtgagca
       61 ttttgctgcg tttgtctgcc aatccggcct cctgataacg ccgggacggg cgggaaatct
      121 tttcccggtc ggcgcggtgt tggacaagcc gcaggcgcgt cagtggtttt agcgggtttc
      181 ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc ttagtatggt
      241 aaatcgaagt aaaaattcag ggaagtgctt catgtggcag gtaaaaaatt cagctgtggg
      301 gcgtaagtgc gaaatgctag agacgatgaa atgacgcctc ctcgctcact cggtcgctac
      361 gctcctgccg tgagactgcg gcgggcgtta cctgctcaag aatgacgaga tgaatagcgc
      421 tcaaatcaag aaggaagggg aaaaggatcc ggcgttgccg tttttccata ggctccgccc
      481 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
      541 ataaagatac caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt
      601 tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca cgcctgacac
      661 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
      721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccgaaa agacacgact
      781 taacgccact ggcagcggcc actggtaagc aggatgtgcg agagatgtat cgagagttct
      841 tgaagtggag gccttagtga ggctacactg gaaggacagt atttggtgac atgtctcgca
      901 caagactgtt accacggttc agaaacttgc cggtttactg aaccttcgaa aaaccacctt
      961 gccgggtggt tttttcgttt acagggagag agattacgac gagagtaaaa cgatcacaag
     1021 gagatctgac cgtttaccag tgatttggct tcgttttggt ttccactgaa caccaaaata
     1081 gcactttttg caaaaagatg agcagtgata gagttgtatt ctgtatgtgt agaagggctt
     1141 ggtatgtaac ctgaactctg gattggttac atatttgtag gctacttgtc ggaaaccaga
     1201 tttcgacaat gcgttgttaa ggtcaaagac ctgtcccgaa ccgaaatgct aagggaatgc
     1261 cacgaacaac actattacga agaagtcggc tatttagccg gcttttttca tattagggta
     1321 tgaagttttg tacctggatg gctcttccta tcataagcag taatgatgct gtagtacggg
     1381 tcttcgccgt ttggtttttt caattctagt atcatcatcc ccgttcctga ctctacaaca
     1441 atcgctttgt tttggccttt tttaggatct atgagcactt ctgccccagc taataatatg
     1501 ctctccaaaa agatgacaat atcctcagcg gaagtagctc ctatctcgac acgatgcttg
     1561 tcccatatat gcctaaggcc ataggctccc tcgagacttt ttgtcctttt gttgtaaaaa
     1621 ccggaatctc ctagcttgag gatgattttc cctgcgggaa agtctgcatt catgcttggc
     1681 agttctagaa aaaattcact tccatcacac ttagccactc gtgcattttt tgttttctta
     1741 tcattaagaa cagcttcaac tatatcgcgt ggaagtaaca agttcacaaa ctacacctct
     1801 atagccttca ctattatgtt taggcgttaa atcccttttt gaagggctga ttctaacact
     1861 gacgtaatga gcaatttcca gtaaatcttg atagaaaaaa ccagttgtat ttaccataac
     1921 attccttgcc cgcaccgctc cggacgggtt cagcgtgaaa gcggcgttag cccggtaaaa
     1981 ccggcagtaa actccccgct aaacagcgca ttctgcgcct gataaatttc acgtggctgc
     2041 atagccatgc agccacgtga attgctcatg ctttcaatgt atatcctcgt ccaagcccct
     2101 gttttaactg gtttatcaaa aaaccaaaca gaaagcctta ccactaacaa taaaagaggt
     2161 aggaggactg tattttatcg cataaaaaaa catttacaac acgattttta tcgtatagat
     2221 tccataaaaa atgaaattca ctcacctatt aagggtttct atatgaaaga tatgaaagct
     2281 gttgtggttt ttaccgggaa ggatttgaac ataatgagaa ctgagggggg atcaggctac
     2341 tggcatgcca gaacagacag gctcaacgat gccgattacc tcatagcagt aagaaacaga
     2401 agggaaacgt gggcagtaaa agacctggaa cacggcacag cattcctgat cgccaaaatt
     2461 accgggtgtt ttaaaagccc tgattacgac gacaggaacg tcataacctt tgatgaatac
     2521 gcagaaattc acacgccaaa agcatggaaa atgctaaccg atggtcagcg ctatccggtt
     2581 gcttacctga gcgcacagga agctttttta cgtattgggg taacaccgga acaactggaa
     2641 tggaagaaat tccatccttc atccccatcg gttcctaata cagtgattcc tggtctggct
     2701 gaagagaaaa cagaaaaact ctcccttaac gaggcaattg aaagagccaa aaaggatatt
     2761 agcaacgcaa cagggattga tagctcagcc ataacaataa gcatcaagat ctgatgaagc
     2821 gtgaacaaca agatagcagc ctcatgttct agaactgaac ctgtacgcca gaaacagcaa
     2881 gcccatgctt ttccggcgca cagattcaaa gtgacggacc gttgtacgtt cgctgtcgct
     2941 gagatcgttc cagctccagc gctttctgat gctgcgccgc ccgttcctgc tcacgatgtg
     3001 cctctatcac gttcagcctt gtgacaaccg gcgcagctgc tcgctcaaac tctgcacctg
     3061 cctgctcaag cccgtgacgc gctcgctcag cgccgcgttc tcccgttgca taagaccaga
     3121 catcgtccgc cattccgcga aggcgttctc ccactcgtcc agccttttcg agtagtcctg
     3181 ctgtagctgc tctaatgcgc tcagcaactg tttttccagc tccgtcatgt gctatttccc
     3241 cgccagtatc atccagctcg aatcctctcc gaaccgtccc gcttccgggt tcaccctcac
     3301 gcagcggcgt ctctgctctc cgcaggtcga gagctgcaca ccgctgttcc tctcggacag
     3361 catggcctgc gtgtgcttct gctcccggat ggtcgtgtaa ttgtcgagta tctgctgccc
     3421 ctgccaccac agaatgcttg cgcccgatgc tgtcagcagt gtagacacca agacgatggt
     3481 cagccacgtc tggctgacca tccgcagcat acctttcgtg cgctggctca tggcagcgga
     3541 cagcttcctg tcgtggtcga ggatggcggc gctgattctc ttctcgcttt cgctcagttc
     3601 cgctctgaca gacttctcgt gctcgctgaa tgcggctttc agcatctcgc cggtactctg
     3661 ctgctgcgct ttcgatttct gctctaagtc cttcgccagc gttaaaagac tgttcataga
     3721 tggctccctt cagtcggatg tttcgccccc cgtccgggtc ggcaatgctg atgctgcttt
     3781 ttgtggtgcg taccacctca aaacctgcgc tttccagcgc ctcagtgacg tcctggcggg
     3841 ttttcagctc cccggacgag gctaaggcca gtaaaccccg tgtaattgcc tgagcggctt
     3901 cctgcttcgt ttccggcagc gcggacggcg tgaccagcgc ccgccggttt tcaggcgcgt
     3961 tcgggtcatg cagccccagc ctgccgttca cgatggtctg ccaggcatca atgcgcggac
     4021 ggtcggcgcg gtcgtaatac ggctggagac gcttgccggt cagcagctcc gtgttcggga
     4081 tcaggaagtt cagctccagc cgccccttat cctggtgctc aacccacagc acgctgtact
     4141 ggtctttatc gagtccgggc atcagaaccc gttcaaagct cgccatcagc ttttcgcgct
     4201 gcccgggcgg taaatccttt tcggcaaagg acaggacgcc ggatgtgtac ttttttgcgt
     4261 agggcgaggc gtcgataagc tccctgactt cttccggctt cccctgcaga acggtggcgc
     4321 cgtcgcgctg tcggtctttc cccagcagat aatccaccgg accggcgcca ccgccgcgcc
     4381 ctcgcgggtg aaacttaacg atcatcgtct gcaccctttt ccagcacggc atgccgcagc
     4441 cgctcgagtc cggcatctat ggccatcagc gcggcgacga cctgcacgcg gtcgtgcccg
     4501 ctgccaccac cggcgttaac ctgccgggca atctggttga ggttgttccc catgccggca
     4561 agctgacgaa gcagcgccgg cgagagcgag ggaagcttgc ccgcgcgggc gggtttctcg
     4621 tccaggcacg tctggcgcat ccaggctgca agctgtt
//
