LOCUS       NZ_CP099724             6647 bp    DNA     circular CON 10-JUL-2023
DEFINITION  Escherichia coli strain EC21GDE043 plasmid pGDE043-3, complete
            sequence.
ACCESSION   NZ_CP099724
VERSION     NZ_CP099724.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29354608
            Assembly: GCF_024125695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Huang,Y., Liu,C., Tang,B. and Guan,C.
  TITLE     Complete genome sequence of mcr-1-harboring E. coli isolated from
            Guangdong
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Huang,Y., Liu,C., Tang,B. and Guan,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Mar-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/10/2023 04:28:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,357
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 1 of 285
            Pseudo Genes (frameshifted)       :: 89 of 285
            Pseudo Genes (incomplete)         :: 191 of 285
            Pseudo Genes (internal stop)      :: 64 of 285
            Pseudo Genes (multiple problems)  :: 56 of 285
            Pseudo Genes (short protein)      :: 5 of 285
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21GDE043"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="pGDE043-3"
                     /geo_loc_name="China:Guangdong"
                     /collection_date="2021-03"
     gene            join(6578..6647,1..89)
                     /locus_tag="NH569_RS26625"
                     /old_locus_tag="NH569_26625"
     CDS             join(6578..6647,1..89)
                     /locus_tag="NH569_RS26625"
                     /old_locus_tag="NH569_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            587..814
                     /locus_tag="NH569_RS26630"
                     /old_locus_tag="NH569_26630"
     CDS             587..814
                     /locus_tag="NH569_RS26630"
                     /old_locus_tag="NH569_26630"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(811..2379)
                     /gene="cea"
                     /locus_tag="NH569_RS26635"
                     /old_locus_tag="NH569_26635"
     CDS             complement(811..2379)
                     /gene="cea"
                     /locus_tag="NH569_RS26635"
                     /old_locus_tag="NH569_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2639..3076
                     /locus_tag="NH569_RS26640"
                     /old_locus_tag="NH569_26640"
     CDS             2639..3076
                     /locus_tag="NH569_RS26640"
                     /old_locus_tag="NH569_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3157..3576
                     /locus_tag="NH569_RS26645"
                     /old_locus_tag="NH569_26645"
     CDS             3157..3576
                     /locus_tag="NH569_RS26645"
                     /old_locus_tag="NH569_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3894..4127)
                     /locus_tag="NH569_RS26650"
                     /old_locus_tag="NH569_26650"
     CDS             complement(3894..4127)
                     /locus_tag="NH569_RS26650"
                     /old_locus_tag="NH569_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_167817990.1"
                     /translation="MTELETHLLNALEQLQQDYMQWLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4134..4652)
                     /locus_tag="NH569_RS26655"
                     /old_locus_tag="NH569_26655"
     CDS             complement(4134..4652)
                     /locus_tag="NH569_RS26655"
                     /old_locus_tag="NH569_26655"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4690..5121)
                     /locus_tag="NH569_RS26660"
                     /old_locus_tag="NH569_26660"
                     /pseudo=""
     CDS             complement(<4690..5121)
                     /locus_tag="NH569_RS26660"
                     /old_locus_tag="NH569_26660"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5330..5653)
                     /locus_tag="NH569_RS26665"
                     /old_locus_tag="NH569_26665"
     CDS             complement(5330..5653)
                     /locus_tag="NH569_RS26665"
                     /old_locus_tag="NH569_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5717..5908
                     /locus_tag="NH569_RS26670"
                     /old_locus_tag="NH569_26670"
     CDS             5717..5908
                     /locus_tag="NH569_RS26670"
                     /old_locus_tag="NH569_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(5994..6081)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          6092..6647
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3791..5340)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaggcacgc cttaacgcca ctggcagcag ccactggtaa ccggattagc agagcgatga
       61 tggcacaaac ggtgctacag agttcttgaa gtagtggccc gactacggct acactagaag
      121 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      181 ctcttgatcc ggcaaacaaa ccaccgttgg tagcggtggt ttttttgttt gcaagcagca
      241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgcg
      301 atccccgtca gtttagaaga ggaggatggt gcgatggtcc ctccctgaac atcacgtata
      361 tagttagcct gacatccaac aaggaggttt atcgcgaata ttcccacaaa aaatcttttc
      421 ctcataactc gatccttata aaatgaaaag aatatatggc gaggtttaat ttatgagctt
      481 aagatactac ataaaaaata ttttatttgg cctgtactgc acacttatat atatatacct
      541 tataacaaaa aacagcgaag ggtattattt cctcgtgtca gataagatgc tatatgcaat
      601 agtgataagc actattctat gtccatattc aaaatatgct attgaataca tagcttttaa
      661 cttcataaag aaagattttt tcgaaagaag aaaaaaccta aataacgccc ccgtagcaaa
      721 attaaaccta tttatgctat ataatctact ttgtttggtc ctagcaatcc catttggatt
      781 gctaggactt tttatatcaa taaagaataa ttaaatccct aacacctcat ttatagtatt
      841 aagtttattc ttatcaatat aggagcatag aattcctgta acaatagcaa taccccaaat
      901 acctaatgta gttccagcaa gcaagctaaa aagtaaagca acaacataac tcacccctgc
      961 atctgcagct ttcttctcta atgtaagaaa tagtggcttc cagtcacctg tatccttaat
     1021 ttttaggata tcagatacca catcatatcc aaaagaaaca tgccccgtaa tcttcaagta
     1081 cttggcaaac tgatctaaat gtttagccca gtcatcatac ttcaccgatg ccaacgcatt
     1141 aaaaatagca tcacgatcgg ctttgctgaa tttcttattt aaaacatcct tgtatttttc
     1201 aaaagcagcg agagcttcat tcacattgcc gattttctta cctttagact tatcagcaag
     1261 ttcctgtgcc attttcgaat atttttcacc atatttttca gtcagcgttt gataaaagct
     1321 aactgttgca tcaacagcat ccttaatctg tgaattaagg agattattct gtgctttttt
     1381 caaattttct tcagcttcat gaacacgagc gataccggca ttacgattat tactgacctg
     1441 agaaatagcc ttctggatct gagttatatc agcatttatc cggttaatac gtgtttctga
     1501 tgctgttacc tgtttttgtt tttcttctct aatcttaccg gccccaaccc gtcgtctggt
     1561 tgcttcaaaa aaaggacggt tctgaagcgg atcattggct cttggtgata gttttttgac
     1621 cagctcatcc agttctttat atttagcgga tgcctgagcc agttcatttc gttttccagc
     1681 gagcgttttc atttctgcat cacgggcatg gatactggag cttaaacgag aattgagagt
     1741 cttaatctct ccatccattt tcaccacttc agattgtgca gcagaaagtt ttttttgggc
     1801 gatctcaaca gctttagctt cttcactcaa tgcagccagt cgtttctctt cagcttcagc
     1861 cagtttcaac tggcgttctg tttcagcctt ctcccgttca atctctttac gtcgttgttc
     1921 tgcttcctga aaagcctttt ctgctgcttc cgcttcttta cgggcttttt cttctgcttt
     1981 cgcaaggcgc aaacgctctg cttccgcctg catagctgca ttattagcat gagcaagctc
     2041 tgttgctgaa ggcgtacgtg aggcattgtg acgaagagcc tcattcacga tatccttcag
     2101 gcgctgagtc agcgcatccc tgtttgcctt tgctttcgcc tgtgcttccg ctgcagcttt
     2161 tgcccgggca gcctgctctg cctgtgtttt ctttaattga gcagtagacc atttagcagt
     2221 tgcatgaata gctgcagaac tttcactttt actgcctcct tttccacctc cgccgccaga
     2281 gccactcccg tcaggagtac cattcaaaag agtaataatt acctgtccct tatcatcata
     2341 aggaacacca tctttatagt acgctaccgc ggtttccatt ataaaatcct ctttgacttt
     2401 taaaacaata agttaaaaat aaatactgta catataacca ctggttttat atacagcata
     2461 aaagctacgc cgctgcattt tccctgtcaa gactgtggac ttccattttt gtgaaaacga
     2521 tcaaaaaaac agtctttcac accacgcgct attctcgccc gatgccacaa aaaccagcac
     2581 aaacattacc gttctcagac ctcattatgt tttactgaaa ctatgagatg agacatctat
     2641 gggacactgt cactttatgg catggcacac actccgggac gcactaaaaa tgacaggcag
     2701 atcgcgttca cagttttacc gtgatatgcg cgcaggcctt gtcagttacc gtaccggcag
     2761 ggacggacga cgggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca
     2821 gaatgagaca ccagaaaggc acagtgaggg acatgcagaa aatccacatg atcagcagac
     2881 agaacgcatt ctccgggaac tgaatgagct gaaacaatgc ctgacgctga tgcttgagga
     2941 taaacaggca caggatatgg atcgcagacg ccaggaagca gaacgggaac agctacaaaa
     3001 tgagatagcc cagctcaggc aggcactgga actggaaaag aaacggggat tctggtccag
     3061 gttgttcggt cgctgaacgc tgtcagagac tgatgataaa atagtcttcg gataataact
     3121 caccgagaat aaatacttta aggtagggag acactcatga gacgtaccgg aaacaaactt
     3181 tgtcttatcg ccatgataac agcaacagta gctctcacag cctgtacccc aaagggcagc
     3241 gtggaacaac atacccggca ttacgtatat gcttctgatg acggttttga tcccaacttt
     3301 tccacccaaa aagccgacac aacacgaatg atggtgcctt tttttcggca gttctgggat
     3361 atgggagcta aagataaagc gacaggaaaa tcacggagtg atgtgcaaca acgcattcag
     3421 cagtttcaca gccaggaatt tttaaactca ctccggggca caactcaatt tgcgggtact
     3481 gattaccgca gcaaagacct taccccgaaa aaatccaggc tgctggctga cacgatttct
     3541 gcggtttatc tcgatggcta cgagggcaga cagtaagtgg atttaccata atcccttaat
     3601 tgtacgcacc gctaaaacgc gttcagcgcg atcacggcag cagacaggta aaaatggcaa
     3661 caaaccaccc gaaaaactgc cgcgatcgcg cctgataaat tttaaccgta tgaataccta
     3721 tgcaaccaga gggtacaggc cacattaccc ccacttaatc cactgaagct gccatttttc
     3781 atggtttcac catcccagcg aagggccatc cagcgtgcgt tcctgtattt ccggctgacg
     3841 ctcccgttcc agggataaca catgttcgcg ctcctgtatc agccgttcct ctcttatctc
     3901 cagttctcgc tgtataactg gctcaagcgt tctgtctgct cgctcaagtg ctgcacctgc
     3961 tgactcaact gcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac
     4021 atcttctgca attccacgaa ggcgctctcc cattcgctca gccactgcat atagtcctgt
     4081 tgcagctgct ctaaggcgtt cagcaaatgt gtttccagct ctgtcactct gtgtcactcc
     4141 ttcagatgta cccactcttt cccctgaaag ggaatcacct ccgctgattt cccgtacgga
     4201 aggacaagga atttcctgtt cccgtcctgc acaaactcca cgccccatgt cttcgcgttc
     4261 agtttctgca atgtctcttc ctgcttcctg atttcttcca ggttcgcctg tatcctccct
     4321 ccaagatacc agagcgtccc gccactcgcg gtaaacagga gaaagactat ccccagtaac
     4381 atcatgcccg tattccctgc cagctttaac acgtccctcc tgtgctgcat catcgcctct
     4441 ttcacccctt cccggtgttt ttccagcgat tcctctgtcg aggctgtgaa cagggctata
     4501 gcgtctctga ttttcgtctc gtttgatgtc acagcctcgc ttacagattc gccgagcctc
     4561 ctgaactcgt tgttcagcat tttctctgta gattcggctc tctctttcag ctttttctcg
     4621 aactccgcgc ccgtctgcaa aagattgctc ataaaatgct cctttcagcc tgatattctt
     4681 cccgccgttc ggatctgcaa tgctgatact gcttcgcgtc accctgacca cttccagccc
     4741 cgcctcagtg agcgcctgaa tcacatcctg acggcctttt atctctccgg catggtaaag
     4801 tgcatctata cctcgcgtga cgccctcagc aagcgcctgt ttcgtttcag gcaggttatc
     4861 agggagtgtc agcgtcctgc ggttctccgg ggcgttcggg tcatgcagcc cgtaatggtg
     4921 atttaccagc gtctgccagg catcaattct aggcctgtct gcgcggtcgt agtacggctg
     4981 gaggcgtttt ccggtctgta gctccatgtt cggaatgaca aaattcagct caagccgtcc
     5041 cttgtcctgg tgctccaccc acaggatgct gtactgattt ttttcgagac cgggcatcag
     5101 tacacgctca aagctcgcca tcactttttc acgtcctccc ggcggcagct ccttctccgc
     5161 gaacgacaga acaccggacg tgtatttctt cgcaaatggc gtggcatcga tgagttcccg
     5221 gacttcttcc ggattaccct gaagcaccgt tgcgccttcg cggttacgct ccctccccag
     5281 caggtaatca accggaccac tgccaccacc ttttcccctg gcatgaaatt taactatcat
     5341 cccgcgcccc ctgttccctg acagccagac gcagccggcg cagctcatcc ccgatggcca
     5401 tcagtgcggc caccacctga acccggtcac cggaagacca ctgcccgctg ttcaccttac
     5461 gggctgtctg attcaggtta tttccgatgg cggccagctg acgcagtaac ggcggtgcca
     5521 gtgtcggcag ttttccggaa cgggcaaccg gctcccccag gcagacccgc cgcatccata
     5581 ccgccagttg tttaccctca cagcgttcaa gtaaccgggc atgttcatca tcagtaaccc
     5641 gtattgtgag catcctctcg cgtttcatcg gtatcattac cccatgaaca gaaatccccc
     5701 ttacacggag gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg cccgctttat
     5761 cagaagccag acattaacgc tgctggagaa gctcaacgaa ctggacgcag atgaacaggc
     5821 cgatatttgt gaatcgcttc acgaccacgc cgatgagctt taccgcagct gcctcgcacg
     5881 tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     5941 tctgtgagcg gatgccggga gctgacaagc ccgtcagggc gcgtcagcag gttttagcgg
     6001 gtgtcggggc gcagccctga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     6061 catgcggcat cagtgcggat tgtatgaaaa gtacgccatg ccgggtgtga aatgccgcac
     6121 agatgcgtaa ggagaaaatg cacgtccagg cgcttttccg cttcctcgct cactgactcg
     6181 ctacgctcgg tcgttcgact gcggcgagcg gtactgactc acacaaaaac ggtaacacag
     6241 ttatccacag aatcagggga taaggccgga aagaacatgt gagcaaaaga ccaggaacag
     6301 gaagaaggcc acgtagcagg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6361 aaatagacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6421 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6481 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct
     6541 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6601 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccgg
//
