LOCUS       NZ_CP099578             3125 bp    DNA     circular CON 16-JUL-2023
DEFINITION  Staphylococcus aureus strain MRSA1369 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP099578
VERSION     NZ_CP099578.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29251701
            Assembly: GCF_024172245.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Tukenmez,H., Nye,T.M., Bonde,M., Caparon,M.G., Almqvist,F.,
            Hultgren,S.J. and Johansson,J.
  TITLE     Complete Genome Sequence of the Uropathogenic Methicillin-Resistant
            Staphylococcus aureus Strain MRSA 1369
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Tukenmez,H., Nye,T.M., Bonde,M., Caparon,M.G., Almqvist,F.,
            Hultgren,S.J. and Johansson,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2022) Molecular Microbiology, Washington
            University, 425 S. Euclid Avenue, Hultgren Lab, MPRB Room 10260, St.
            Louis, MO 63110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/16/2023 03:48:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,835
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 42 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 14 of 80
            Pseudo Genes (multiple problems)  :: 15 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099578.1.
            Bacteria and source DNA available from Hultgren Lab, MPRB Room
            10260, 425 S. Euclid Avenue St. Louis, MO 63110.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="MRSA1369"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
     gene            join(2495..3125,1..365)
                     /locus_tag="NHB12_RS14560"
                     /old_locus_tag="NHB12_14560"
     CDS             join(2495..3125,1..365)
                     /locus_tag="NHB12_RS14560"
                     /old_locus_tag="NHB12_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            948..1136
                     /locus_tag="NHB12_RS14565"
                     /old_locus_tag="NHB12_14565"
     CDS             948..1136
                     /locus_tag="NHB12_RS14565"
                     /old_locus_tag="NHB12_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1462..2043)
                     /locus_tag="NHB12_RS14570"
                     /old_locus_tag="NHB12_14570"
     CDS             complement(1462..2043)
                     /locus_tag="NHB12_RS14570"
                     /old_locus_tag="NHB12_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          436..600
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          2..365
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2496..3125
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.521"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ttgactatcg acctgttgct aatgttaaag cgatcaaacc gaatagaaaa ggcgataaag
       61 acattgaatc ggcaatcaaa gagacctcaa aatattcggt taaatcatct gattttttaa
      121 ctgatgatga tgaaaaaaat caagaaattg taagtgattt agaaaaaggt ttgtatcgaa
      181 aacgtatgtt aagttatggt ggattgctta aacaaaaaca taaaatttta aacttagacg
      241 atgtcgaaga tggtaatttg attaatgcaa gtgatgaaga taaaacaaca gacgaagaag
      301 aaaaagcaca ttcaattacc gcaatttgga atttcgaaaa gcaaaattat tatttaagac
      361 attagtgttg actaatgtct tttttgttga ttttttataa aaaagtactg tcttattttt
      421 gtgacaaatg ctgtatgtag tgtcacaaaa ataagacaaa cgcaatatat tgtgtcacaa
      481 aaataagaca gtacagcttt gtatgatatc actttaaaat accttaaaac ccctggaatt
      541 tctggctttg ccagacctat catttttgaa tgatagcaaa ttctccttat gctcttacgg
      601 agtttttaga gaaaaattaa aaattctcga tttttgataa aaaacgccct gcaggaattt
      661 agaaaaacat gtggaagttt taaaggattt tatgctaatt tttaatttgc atgtaaatgg
      721 gcagtgttaa aaaattagtc actggttttg ctgaaatttt tgtttgtgaa attaccatat
      781 atttatttgg ctcatatttg cgttttaaga gcttgtatat atttttaagt taaaaaccta
      841 tatgataccc cctaaacctt taaaataccc ccttaaaact caaaataaag gtatttataa
      901 tttgagtaag tcttgtgata aagtttttta aaaaaggagt gtttattttg aataaaaaca
      961 cagtgttaga tgaaggttac gttatagcaa ctattttaaa tgttttcttt ttacttggat
     1021 taatttttat atctcatttg gaaaatttat atatattaat tccgtatgtt attttgatgg
     1081 gaataaatgc tatttatttg gttgttaagg ttatgaattt caaaaagaat aattagaata
     1141 tttgagggga ttttgaaacg agtttcttct tgttttcaca ctgttttttt attcctattg
     1201 gtgttgttgc ttactttttg tttttctata aagatgatgc ttttgacgag atggaagaaa
     1261 aatatgatta tcatgaagat aataaaaaat agacgacgca tttatgccga gaaaatttat
     1321 tgatgttgag aagaaccctt aactaaactt gcagacgaat gtcggcatag cgtgagctat
     1381 taagccgacc attcgacaag ttttgggatt gttaagggtt ccgaggctca acgtcaataa
     1441 agcaattgga ataaatagta tctaaagtaa cccgtattta agattacatt gtacatcctt
     1501 cgaaaccata gtagctgcga ttccagctag gtttcctttt atcccaattt tagctaattt
     1561 ttttgctacg ccctttaagt ttttggcttt aacatcttca aaaactgctg tgattatgtt
     1621 tccagttaaa agttctccat atgagtttaa aacttcagaa tagaaacagt caccgctatt
     1681 tttgtagttc ccaccaactg ctctaggtga tattgaacta gttttctttt tattctctat
     1741 gtcgtttttt agtttttgga atccttcagg aacatatcca tatcgagatt tggcattttc
     1801 tagattaaca tctttagctg ttccgtcttt atctaaaata ataattttgc catatatgta
     1861 ttttagttct ttttcaattt ctttttcgtt taaagtttca acgtttttat ctgcaatgtt
     1921 atttttgttt tgggcttgag cttctttgaa attaaaatcc aagaccattg aaagtatggt
     1981 taaaatactt aaagatacta ctaaaatctt tcttagtgat atactgtttt gaattgcatt
     2041 cattgtaaat gcccccttgt tttgttattt aaatgaattc aatataatat taacatataa
     2101 aaacaaagga gtttaatatg aataagtttt tttacttttt gattatttta ttatctgtat
     2161 ttttaatttt taattttgtt aattcgattt tatttgataa tccttttgac tttttagaaa
     2221 gttttattta ttcattggtt ttcacattaa tttatttagg tttagagggt cataaaaaga
     2281 agaaggaata ggttgttttt tgaaacgagt gtgaacgagt ttcttcttgt cttgatacta
     2341 tatagaaata actcgatttt atatatatag ctgtaactgt tgatattaca gtgtttaaac
     2401 gtgtttttgt gcgtgaaagg aaaatttgac aataaaaaac cccagttata ttattaaggt
     2461 gtcgaatctt aaataatact gggggtcttt ttatatgcaa tataatacta ctagaagtat
     2521 aaccgaaaat caagataata aaacgttaaa agatatgacg aaaagtggga aacaacgccc
     2581 atggagagaa aagaaaatag ataatgtaag ctatgcagat atactagaaa ttttaaaaat
     2641 caaaaaggct tttaatgtaa aacaatgtgg taatatttta gaatttaagc caactgatga
     2701 aggctatttg aagttacata agacatggtt ttgtaaatca aaattatgtc cggtttgtaa
     2761 ttggagacgt gctatgaaaa atagttatca agctcaaaaa gtgattgaaa aagtaattaa
     2821 ggaaaagcca aaagcacgtt ggttgttttt aacactttca acaaaaaatg cgatagatgg
     2881 agatacttta gaacaaagtt tgaagcatct aactaaagca tttgataggt tgagtagata
     2941 taaaaaggtt aaacaaaatc ttgttggatt tatgcgttca acagaagtta ccgttaataa
     3001 aaatgacggt agttataatc agcacatgca tgttttgtta tgtgttgaaa atgcatattt
     3061 tagaaaaaaa gagaattata taactcaaga agaatgggtt aatttatggc aaagagcatt
     3121 acaag
//
