LOCUS       NZ_CP099408             3325 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 33221 plasmid pSKLX33221-5, complete
            sequence.
ACCESSION   NZ_CP099408
VERSION     NZ_CP099408.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28986224
            Assembly: GCF_023913555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Liu,R.
  TITLE     MCR-9- and KPC-2-Co-Producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Liu,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) School of Medicine, The First Affiliated
            Hospital of Zhengzhou University, Qingchun Street, Shangcheng
            District, Hangzhou, Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 15:00:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,138
            CDSs (total)                      :: 6,017
            Genes (coding)                    :: 5,826
            CDSs (with protein)               :: 5,826
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 73 of 191
            Pseudo Genes (incomplete)         :: 126 of 191
            Pseudo Genes (internal stop)      :: 26 of 191
            Pseudo Genes (multiple problems)  :: 30 of 191
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099408.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="33221"
                     /isolation_source="cerebrospinal fluid"
                     /db_xref="taxon:573"
                     /plasmid="pSKLX33221-5"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2015-08"
     gene            759..908
                     /locus_tag="NE845_RS30590"
                     /old_locus_tag="NE845_30590"
     CDS             759..908
                     /locus_tag="NE845_RS30590"
                     /old_locus_tag="NE845_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310068.1"
                     /translation="MPSEYDEKLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1389..1580
                     /locus_tag="NE845_RS30595"
                     /old_locus_tag="NE845_30595"
     CDS             1389..1580
                     /locus_tag="NE845_RS30595"
                     /old_locus_tag="NE845_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1580..2002
                     /locus_tag="NE845_RS30600"
                     /old_locus_tag="NE845_30600"
     CDS             1580..2002
                     /locus_tag="NE845_RS30600"
                     /old_locus_tag="NE845_30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032451468.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGPLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2798..3319)
                     /locus_tag="NE845_RS30605"
                     /old_locus_tag="NE845_30605"
     CDS             complement(2798..3319)
                     /locus_tag="NE845_RS30605"
                     /old_locus_tag="NE845_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310069.1"
                     /translation="MSDLQNKLVFSESPRVELGSNFFINVPVILQYKDTPLISVESAVD
                     ASFTTSIPIYHNDGTYLALAKGTQLYRTEEGRKAGVTLRYEDKVTVCLLNGKEVFELRR
                     QSAAALKAEAELYVPDGKLISYRGSSPKLFSTENNQLAIRGLVMSGSTFSGCKIGILLD
                     ENSIAIGVNP"
                     /name="hypothetical protein"
ORIGIN
        1 atcctttttt tgtcaaaaaa aacctaatgc ccccgtggtg ggtatataaa aatcacgggg
       61 agcgtagcga ccttcgtcca gctcgtttct tgtgtgtctc accctgcaaa aggttcgatt
      121 gtgtgggagt agtgtccgac gttttcaatc acactactcc gcttactact ttcttagaca
      181 ctcgttttat caggtgcttg ttatcgtaca gcgagacggg gtagagctta actcttttgc
      241 ggttaagtta catatccacc aattgcctgt ttatcctgcc ggagtccggg gcgtagacct
      301 gagtgtctaa ggtgcagata ttattttaat gtcgttgaat ggtattaagc tggtagtgac
      361 aatgctagcc agccctgcta ggatttgatt tctgacgtct attcctggca ggtatctgcg
      421 aagaattaga gtaccgaagc cctgtgattc tgtcacgggg cttttgtaca tttaaagcca
      481 gagaatcaaa aacatcccca caaagacacc ttcaccgctc gccgcagccg aacgaccgag
      541 cgtagcgagt cagtgagcga ggaagcggaa gagccactgc cttggcatgg ttcacaggac
      601 actttggtca tgcatatttc acaccctgca tggcgcactt cacctgacct ttcgctcatg
      661 gcaacatatc aagccagtct accgctagtg ctacgtgacg ggttcagggc tgcaccccta
      721 aaaccgctaa caacctctca gctactggat atcaccccat gccatctgaa tatgatgaaa
      781 aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat ttaatggcgc
      841 aggttactga agagaacctg catgcccctg tggattctgg gccgcctgtc ggccgggaag
      901 cgttatagct ttttcgcttg cccgcgtacg tggtaatacg ccggtattac attttatttc
      961 tttattttca tgcgattaca ggtgattgta atacgttgcg tattacataa acaaacggag
     1021 atttcagttt ttacctttta attcatgcgg ttgcgatgaa agtcgcggct gcgttaggtg
     1081 tatgaccatt taaggggcaa aaaattatca ttctggccgc gtgatctgcg caggttaagg
     1141 agcatcggcg acgaaaaagc gtctttaact gcttcgttca tgcaagcatg aacggattat
     1201 ttttccggac tacaggtaaa ctacaggaag actacaaaag ttgatgactt aatcctgatt
     1261 tttagtgcga tgactacaga taaactacaa aataactaca ggattacctc agatccgttc
     1321 tctgaaccat tcgtacatcc gaccccctta ccggttttac actgagcact gatgctctga
     1381 gatcccttat gacgaaacaa cagaaaacag cgctgaacat ggctaaattc atccagaatc
     1441 agtctctgct cctgctggag aagcttaacg agcttgatct cgatgctgag gccgaccttt
     1501 gtgagaaact ccacgatgat gcggagcatc ttttccgtac actgtcctcg agactggatg
     1561 cattacagga tggtaactga tggccagagt gtctattagt gaagcagcac ggcttgttaa
     1621 ggttagtcgc cctactattt acaagatgat taacagtggt aagttaagtt acacctctgt
     1681 tgtaaagcac gggaagggta ttaaagttat tgatacatca gagcttatta gagtatttgg
     1741 tcctcttgat ggtgtaatcg atgctgtaaa atatgatgta aaaagtgacg cagaatctac
     1801 aggtattaac agcgttggtt tacatgattt acaacataga atcgctttac ttgaagctga
     1861 aaacgatggt cttaaagggg ctgttaaggc tcgagatgag catattgatt cccttcgtca
     1921 ggctatgcag cttctggagc acaagcacga accttcttca ccaccccatt ccccgtggtg
     1981 gaaattctgg aaaaaatcct gatcccgcca tgcttgagtt ttcctctccg gccatgccgg
     2041 ggcgttcgtg aaccttccca aaaatcgcca gatctctgaa cgctcctcac actaaaaata
     2101 tccacacccc acgcaaaatt aaatttttgc agataactca ttaatatcag tcagttggtt
     2161 gttattaaat tacgcagtat taccttctgt aaggtcaatc caagtcgtcg gtgattcgtg
     2221 tacgacttgc cgacgactcg tcatagagtc atcgacgact cacttaagac tatcagtaac
     2281 ttatgacgac ttgactctag agtcgaatcg ttattttggt gtttttttga ccttgacgac
     2341 tctagagtca agtcacgagt cgaatcgccg gtgaatcgtg tggtgagtcg tccggcgact
     2401 tgccataacg tgattgccgg ccgtcgacga acccgattcc tgatcgtgct gaaaaccggt
     2461 tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt
     2521 tttcggatag gaagaacgcc ggaaacagga cttaccctgg ctgaatggcg tgaatttccg
     2581 cgctatgcac ttgcgcgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg
     2641 cgggttttcg ggtggtttgt tgcctgtttt accgctttcc cgtcagaaac gccctgaggg
     2701 cctctcaggt ggtgctgata acggatgtta tggtaaatac aggtgagcgg gataaaccat
     2761 catcttttat tgcgtgcgca agaaataacc aacgcggcta cggatttaca ccaatcgcta
     2821 tgctgttttc atctagcaat atccctattt tacacccgct gaatgttgat ccggacatca
     2881 ctaaaccgcg tatagctagt tgattgtttt ccgtgctgaa caactttggc gaggatcctc
     2941 gataacttat tagctttcca tcaggaacat aaagctcagc ctctgctttt agtgcagctg
     3001 cgctttgtct tctgagctcg aatacctctt tcccgttaag aagacaaaca gttaccttat
     3061 cttcataccg gagagtcact cctgcctttc taccttcttc agtacggtag agctgcgttc
     3121 ctttagccag ggccaggtat gttccatcgt tgtgatagat agggatgctg gtagtgaaac
     3181 tagcatcaac cgcactctca acactaataa gtggggtgtc tttatactga agtattactg
     3241 ggacgttgat gaaaaaatta gaaccaagct caactcgagg gctttcacta aatacgagtt
     3301 tattctgcaa atcactcatg gatac
//
