LOCUS       NZ_CP099354             8401 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia marmotae strain RHB02-E4-C07 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP099354
VERSION     NZ_CP099354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488490
            Assembly: GCF_029717905.1
KEYWORDS    RefSeq.
SOURCE      Escherichia marmotae
  ORGANISM  Escherichia marmotae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB02-E4-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029717905.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:21:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,620
            CDSs (total)                      :: 4,502
            Genes (coding)                    :: 4,336
            CDSs (with protein)               :: 4,336
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 53 of 166
            Pseudo Genes (incomplete)         :: 120 of 166
            Pseudo Genes (internal stop)      :: 24 of 166
            Pseudo Genes (multiple problems)  :: 30 of 166
            Pseudo Genes (short protein)      :: 3 of 166
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Escherichia marmotae"
                     /mol_type="genomic DNA"
                     /strain="RHB02-E4-C07"
                     /isolation_source="Pooled sediment sample collected from
                     floor of pig farm"
                     /db_xref="taxon:1499973"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            198..638
                     /locus_tag="NFJ26_RS22965"
                     /old_locus_tag="NFJ26_22865"
     CDS             198..638
                     /locus_tag="NFJ26_RS22965"
                     /old_locus_tag="NFJ26_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(1405..1554)
                     /locus_tag="NFJ26_RS22970"
                     /old_locus_tag="NFJ26_22870"
     CDS             complement(1405..1554)
                     /locus_tag="NFJ26_RS22970"
                     /old_locus_tag="NFJ26_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2125..2283
                     /locus_tag="NFJ26_RS23125"
     CDS             2125..2283
                     /locus_tag="NFJ26_RS23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2743..3558
                     /gene="sul2"
                     /locus_tag="NFJ26_RS22975"
                     /old_locus_tag="NFJ26_22875"
     ARG             2744..3555
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3619..4422
                     /gene="aph(3'')-Ib"
                     /locus_tag="NFJ26_RS22980"
                     /old_locus_tag="NFJ26_22880"
     ARG             3620..4419
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4422..5258
                     /locus_tag="NFJ26_RS22985"
                     /old_locus_tag="NFJ26_22885"
     CDS             4422..5258
                     /locus_tag="NFJ26_RS22985"
                     /old_locus_tag="NFJ26_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            5564..5806
                     /locus_tag="NFJ26_RS22990"
                     /old_locus_tag="NFJ26_22890"
     CDS             5564..5806
                     /locus_tag="NFJ26_RS22990"
                     /old_locus_tag="NFJ26_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5838..6488)
                     /gene="tetR(A)"
                     /locus_tag="NFJ26_RS22995"
                     /old_locus_tag="NFJ26_22895"
     CDS             complement(5838..6488)
                     /gene="tetR(A)"
                     /locus_tag="NFJ26_RS22995"
                     /old_locus_tag="NFJ26_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            6594..7793
                     /gene="tet(A)"
                     /locus_tag="NFJ26_RS23000"
                     /old_locus_tag="NFJ26_22900"
     ARG             6595..7790
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7760..7948)
                     /locus_tag="NFJ26_RS23005"
                     /old_locus_tag="NFJ26_22905"
                     /pseudo=""
     CDS             complement(<7760..7948)
                     /locus_tag="NFJ26_RS23005"
                     /old_locus_tag="NFJ26_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7992..8381)
                     /locus_tag="NFJ26_RS23010"
                     /old_locus_tag="NFJ26_22910"
     CDS             complement(7992..8381)
                     /locus_tag="NFJ26_RS23010"
                     /old_locus_tag="NFJ26_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             4423..5255
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1590..1763)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1687..2292
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.431"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttaaaaacag caaaaactgt atttataaca atgtgtttat atacagtatt cgaaatacta
       61 cgcccgtcaa aaccacaccg tcaacaggtt ttttgtgtat ttttcacgcc tcacgggcag
      121 cctcagacct tttgtgttaa aacagtaata tgttctgctg ttacaatacg gtacacacag
      181 gagacactgt cacttttatg gcatggcaca cagttcaaga gacactgaaa ttaacaggaa
      241 agtcgcgctc gcaattatat cgtgacatgg ctaaaggcct tgtcagctat cgcacaggaa
      301 aaaatgatcg ccgggagttc gagaccagtg aactgattcg ggcctatggc gagctgcgca
      361 cttctgagac accaaaaagg cacaaggagg gacactccaa atctgtcgtt agccaggcgg
      421 aaaatgctca gctagacgtc attcagcaga aactggacgc tctgcagcag actgttaccc
      481 taatgcttga agacaagatg gcgcgggaag cagaaaaccg cctgcgaaat gaggagaggg
      541 aacaactaca ggccgaaatt agccgtctgg ctgacgcgct ggcacaggaa aaaaagcggg
      601 gattctggtc gcggctgttt cgctccggtt cagagtaaca gtgcaggtga ctctgattta
      661 ccataacatc tgttatcagc accgcctgag aggccctcag ggcgtttctg acgggaaacc
      721 ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac
      781 ggcatgcatg agtatgcgca caagtgcata gcgcggaaat tcacaccatt cagccggggt
      841 aagtcctgtt tccggcgttc ttcccatccg aaaacggtga acggggacgc aatttattgc
      901 gtggccgtga agcgttgccg gcgcagccgg aaaacccgct gtcagcacgt tcaggaaggg
      961 ggtgatgtac aaatggcaca gaggatgaaa gtgacgtatg agctgaagga atgatgtaaa
     1021 agtgatgtat aagtgatgta aaagtgatgt ataagtgatg taaaagtgat gtatgaactg
     1081 aaggagcgat gtaattctgt agtcaggaag aataacccgt tcatgcaggc atgaacgata
     1141 cagtgaggaa cgcttttttt gtcacgatct ccccttaacc tgcgtaaatc acccgccatg
     1201 gttgattatt tttcaacctc aaaatggtca tacacctaac gcagccgcga ttttcatcgc
     1261 aactgcatga attaaaagaa aaaaaaggaa tatttcgttt tcacgagtaa tacccgggta
     1321 ttaaaaatcc actgtaatta catgaaaata aagaaataaa aggtaatacc ggagtattac
     1381 cacgtacgcg ggcaagcgaa aaagctataa cgcttcccgg ccgacaggcg acccaaaatc
     1441 cacaggggca tgcaggttct cttcagtaac ctgcgccatt aaatcaaaaa ggtcatattc
     1501 gccgggaagc tgcgtatcag tcgaatccag ttgttcatca tattcagatg gcatggggtg
     1561 atatccagta gctgagaggt tgttagcggg tttcggggcg cagccctgaa ccagtcatgt
     1621 agcgctagcg gagtgtatac tggcttacta tgttgccacg attgcgaaaa tcagtgaagt
     1681 gcttcatgtg gcaggagaaa aaaggcagca ccggcgcgtc agcagaatat gcgatacagg
     1741 agatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcggct gcggcgagcg
     1801 gtaccggctt acggacgggg acggaaattt cctggaagat accaagaaga cacttagcaa
     1861 agaagagata aggccacggc gaagccgttt ttccataggc tccgcccccc tgacaagcat
     1921 cgcaaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagatcccag
     1981 gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     2041 gtcattccgc tgttatggcc gcggtttctc attccacgcc tgacactcag ttccgtgaag
     2101 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
     2161 ttgtccggta actatcaact tgagtccaac ccggaaagac acgacaaaac gccactggca
     2221 gcagccatta gtaacaggag tgcacgagaa gatagattaa gaggtcttga agtagaggcc
     2281 tgactgcggc tacactgaaa gaacagtttt ggtaatggcg gtctcgtgaa ccctgttacc
     2341 gggttaagca gttccccaac tgacttaacc ttcgaacaaa ccgcctcccc aggcggtttt
     2401 ttcgtttaca gagcaagaga ttacgattca ttgaaaagga tctcaagaag atcctttgta
     2461 gattactatc aattaatctt ccgaacatga caccagtcac tgttgattac ggttaaaccg
     2521 ctccgtaaca ggtttccctc gtattggtga cttgcagcac caaagaagca aaaaagataa
     2581 atatatctac ttattgagag atgatatatt tatttccaaa atgctacaga tacatcatca
     2641 tgaaacggat caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc
     2701 gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc
     2761 attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg
     2821 ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac
     2881 ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg
     2941 cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc gctcgacagt
     3001 tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct caatgatatt
     3061 cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc caaactcgtc
     3121 gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc tggcgacatc
     3181 atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc
     3241 aaacgcaacc gccttgtcct tgatcccggc atggggtttt ttctgggggc tgctcccgaa
     3301 acctcgctct cggtgctggc gcggttcgat gaattgcggc tgcgcttcga tttgccggtg
     3361 cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg tccgggggat
     3421 gtcggggccg cgacactcgc tgcagagctt gccgccgccg caggtggagc tgacttcatc
     3481 cgcacacacg agccgcgccc cttgcgcgac gggctggcgg tattggcggc gctgaaagaa
     3541 accgcaagaa ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga
     3601 aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc gcattctgac
     3661 tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg tgacgggcat
     3721 gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga gcgtgaccgc
     3781 ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg gcaggaggaa
     3841 caggagggtg catgcttggt gataacggca attccgggag taccggcggc tgatctgtct
     3901 ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc tgttcacagc
     3961 ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg acgcgccgtt
     4021 gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga caagagtacg
     4081 ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct cgaccaagag
     4141 cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat ggtggaccct
     4201 aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc agatcgctat
     4261 gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc agatgaagca
     4321 gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga ccgcgaacgc
     4381 cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg ccgcctgttt
     4441 ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt tccagcctcg
     4501 tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg aaacctatag
     4561 aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat gggaggggag
     4621 cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc ggggagcgaa
     4681 tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt gcagcggaac
     4741 taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt ctcccgatcc
     4801 gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca ggttgtcaaa
     4861 ctgactacgt ccacgcggcg attatagccg atcaaatgat gagcaatgcc tcggaactgc
     4921 gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc ggctggctgg
     4981 tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat atgttctacg
     5041 atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag atggcggacg
     5101 cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg tacgcttatg
     5161 ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat ctagctatcg
     5221 cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac ttctcctatc
     5281 ccctgggaac acatcaatct caccggagaa tatcgctggc caaagcctta gcgtaggatt
     5341 ccgccccttc ccgcaaacga cccctggaag ccccgtagcg acgcggagag gggcgagaca
     5401 agccaagggc gcaggctcga tgcgcagcac gacatagccg gttctcgcaa ggacgagaat
     5461 ttccctgcgg tgcccctcaa gtgtcaatga aagtttccaa cgcgagccat tcgcgagagc
     5521 cttgagtcca cgctagatct atctcatctg cgcaaggcag aacgtgaaga cggccgccct
     5581 ggacctcgcc cgcgagcgcc aggcgcacga ggccggcgcg cggacccgcg ccacggccca
     5641 cgagcggacg ccgcagcagg agcgccagaa ggccgccaga gaggccgagc gcggccgtga
     5701 ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg ggcgtctgac
     5761 gcggtggaaa gggggagggg atgttgtcta catggctctg ctgtagtgag tgggttgcgc
     5821 tccggcagcg gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt cctgacaacg
     5881 agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc
     5941 ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt
     6001 tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc
     6061 ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc
     6121 gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc
     6181 gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg
     6241 gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga
     6301 ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc
     6361 cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg
     6421 tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac
     6481 tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga
     6541 taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac
     6601 ccaacagacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc
     6661 tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg
     6721 cccactatgg cattctgctg gcgctgtatg cgttgatgca atttgcctgc gcacctgtgc
     6781 tgggcgcgct gtcggatcgt ttcgggcggc ggccggtctt gctcgtctcg ctggccggcg
     6841 ctgctgtcga ctacgccatc atggcgacgg cgcctttcct ttgggttctc tatatcgggc
     6901 ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata
     6961 tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt ttcgggttcg
     7021 ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc cacgctccgt
     7081 tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc cttttgccgg
     7141 agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg ctcgcttcgt
     7201 tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc ttcatcatgc
     7261 aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat cgctttcact
     7321 gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat tcactcgccc
     7381 aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca ctcatgctcg
     7441 gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg ggatggatgg
     7501 cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa
     7561 tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc
     7621 tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta
     7681 taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc
     7741 cggcgctgcg tcgcgggctt tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     7801 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     7861 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     7921 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     7981 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     8041 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
     8101 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
     8161 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
     8221 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
     8281 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
     8341 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
     8401 t
//
