LOCUS       NZ_CP099337             2345 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia fergusonii strain RHB44-C03 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP099337
VERSION     NZ_CP099337.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488660
            Assembly: GCF_029719985.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB44-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 143x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029719985.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:31:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,652
            Genes (coding)                    :: 4,493
            CDSs (with protein)               :: 4,493
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 57 of 159
            Pseudo Genes (incomplete)         :: 122 of 159
            Pseudo Genes (internal stop)      :: 25 of 159
            Pseudo Genes (multiple problems)  :: 40 of 159
            Pseudo Genes (short protein)      :: 2 of 159
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099337.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB44-C03"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:564"
                     /plasmid="unnamed6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            298..1266
                     /gene="mobV"
                     /locus_tag="NFK96_RS23780"
                     /old_locus_tag="NFK96_23740"
     CDS             298..1266
                     /gene="mobV"
                     /locus_tag="NFK96_RS23780"
                     /old_locus_tag="NFK96_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1330..1656)
                     /locus_tag="NFK96_RS23785"
                     /old_locus_tag="NFK96_23745"
     CDS             complement(1330..1656)
                     /locus_tag="NFK96_RS23785"
                     /old_locus_tag="NFK96_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001070513.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQIDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          290..1263
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ctgctctttc aaaaacgata ttcattgcct gatttcgtca agggcgagcg tagcgagtgc
       61 catttaccct tgacgactca ccggataaaa caggcttggc agtggtggga ggtgacgctc
      121 cgcgacgcct tccacctgcc agaccgccgt tgattttgac ctttggaagc gcagcttccc
      181 gaacgatttt gcgaagcaaa gtctagtgag tacactatca tcttgatagt ggcccgccgg
      241 aggtatcgca atgcgctccc ccgcctggct gaatgaatgc cggaatcagg ggggaacatg
      301 gcgaactacg cgataatgcg ctgcaaaaag ctgacgggaa tgggaagcgt ggccagcgcg
      361 ttgcagcact gctaccgaga gcgtgagacg ccgaacgcta acgctgaacg gacaccagaa
      421 aactactgct ctgtgtccaa atcaacggat caggcaatgg ggcgtgtgcg ggagctgctg
      481 ccggaaaaac gccgtaaaga tgcggtactg gccgttgaat acgttatgac agcaagccct
      541 gagtggtgga aagaggccac accacggcag caggcggagt ttttcgcccg ctctgagcaa
      601 tggctggaga agaaatacgg gaaggatcgt gttgtggcgg cggtggttca ccgggacgag
      661 gccaccccgc acctgtctgc gttcgtggtg ccattgacgc aggatggacg gctgtccgcg
      721 aaagagttta ttggcgggcg gtcaaagatg cgtgaagatc agagcaccta cgctgaatca
      781 gtaaaaaaac tgggtcttga gcgcggcatc gagggaagcc gcgccacgca tcagacagtt
      841 cagcactact acgaatcgat aaaccgtggc acccgtagcc aggtatcgat ctcgccggag
      901 gcgctggagc cgcgtgtatt gcggaagggc attttcacca aagacgtgga agatcaggca
      961 gccatagcca aacgtctaag ccacgcagtg aacgatggct ttgcaggaac catcgctatg
     1021 gcctctcaga gcgcccagaa cgcaaaaaga gcgcgtgagc tacagaagac tatggatgcg
     1081 cagcagaaac gcctacagag cgttacagag ccttttaagg ggctttccag agagcagatg
     1141 accgaaatcc tgatgatggc tcagcggttt aagcagcaaa accaggaaaa ggaaaaacag
     1201 cagcgtattg aacgtgaaaa acagcgacag acgcgatcgc gtggcatggg tggtatggag
     1261 cgttagtttt gatgcgatgc aggatagggc taacgccgca aagtgacgtc atttgcggca
     1321 ttccagagat caggttctgc cgccctgtat aacgctgagg ccgtttttaa gggctttttt
     1381 gtggttgctg acagtacctt tccagccgaa ctggggattt aggcggtacg ttttgctccg
     1441 cccaatcttc ggtccttcca gtatgatccc gaaatcaatc aggtttttta ccgcacgact
     1501 gacatttgtt ttttgcatag tcagcgcttc tgcaatatct atctgcgcga cctggatgta
     1561 gttctcgtaa tccagctctg cgagcaaggc catcagtaca cgcatctggt catggtttag
     1621 ctcattggca atggtaatta gcgcggcttg attcatagta aagtgcctct ggaatgatga
     1681 cttttgtttg ggacgaatca ctgctacaaa accacctaat tcctcccctg tactttggtc
     1741 tacctgcgtg attctccgca tctaaaaatc tccaatttca ggaaaaatag ttatctctca
     1801 agataactaa gttatctcta gagataacta ttgtcaaaaa ctttccgctt gtggcgcggc
     1861 ttccgagaca tccccttctg tatcttatta ctctccgtac tcatacatcg gcaaaatcag
     1921 gcgtttttct gcccgttcgg tgcgaacgaa tttctatgta cgggaagtgg aaattccgct
     1981 tcctcgctca ttcgctcact acgttcggtc atgagtctgc ggcgaaaagt acgattagta
     2041 cttgaggtaa gtacgattag tacttgaggt aagtacgatt agtacttgag gtaagtacga
     2101 ttagtacttg aggtgagtac gattcttctc tctagccctt ggtgtgcgcg ggttcccgta
     2161 tctatatcta tatattaagg gggaaaataa aggctgaaaa acgccatttt taaacatcga
     2221 aaaacgagct tttttgagtc tggagcgtac ggatttctat gtccggggag tggaaattcc
     2281 gcttcctcga acggacagac tcgcggcgat gccgctccct aatccgttct tgcggcgagc
     2341 ggttc
//
