LOCUS       NZ_CP099324             4938 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain RHB45-SO-C08 plasmid unnamed1,
            complete sequence.
ACCESSION   NZ_CP099324
VERSION     NZ_CP099324.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488704
            Assembly: GCF_029721335.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB45-SO-C08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 137x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029721335.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:12:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,294
            CDSs (total)                      :: 4,179
            Genes (coding)                    :: 4,142
            CDSs (with protein)               :: 4,142
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 17 of 37
            Pseudo Genes (incomplete)         :: 22 of 37
            Pseudo Genes (internal stop)      :: 7 of 37
            Pseudo Genes (multiple problems)  :: 8 of 37
            Pseudo Genes (short protein)      :: 1 of 37
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099324.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHB45-SO-C08"
                     /isolation_source="Pooled soil sample collected from floor
                     of poultry farm"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            401..892
                     /locus_tag="NFL40_RS21440"
                     /old_locus_tag="NFL40_21430"
     CDS             401..892
                     /locus_tag="NFL40_RS21440"
                     /old_locus_tag="NFL40_21430"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            899..1114
                     /locus_tag="NFL40_RS21445"
                     /old_locus_tag="NFL40_21435"
     CDS             899..1114
                     /locus_tag="NFL40_RS21445"
                     /old_locus_tag="NFL40_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2043..3329
                     /locus_tag="NFL40_RS21450"
                     /old_locus_tag="NFL40_21440"
     CDS             2043..3329
                     /locus_tag="NFL40_RS21450"
                     /old_locus_tag="NFL40_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            4338..4661
                     /locus_tag="NFL40_RS21455"
                     /old_locus_tag="NFL40_21445"
     CDS             4338..4661
                     /locus_tag="NFL40_RS21455"
                     /old_locus_tag="NFL40_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4051..4223
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(3580..4126)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2..1215
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.087"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4339..4655
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
     Typing          4652..4938
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.958"
                     /coverage="97.0"
                     /evalue="2.19e-55"
ORIGIN
        1 cacgaggaca aggggcgtct ggagctgaac tttctgatcc ccaataccga gctgctgacc
       61 ggcaggcggc tccagccgta ctacgaccgc gccgaccggc cgcgtatcga tgcctggcag
      121 accatcgtga acggcagact ggggctgcac gatccgaacg cgccggaaaa ccgccgggcg
      181 ctggtcacgc cggccggact gccgaaaacg aagcaggaag ccgcagaagc catcacccgt
      241 ggactgctga gcttagcctc gtccggggag ctgaaaagcc gtcaggacgt cactgaggct
      301 cttgaaggtg caggttttga ggtggtgcgt acaacgaaaa gcagtatcag cattgccgac
      361 cctgacgggg ggcgaaacat ccgactgaag ggagccatct atgagcagtc ttttaacgct
      421 ggcgaaggac ttagagcagc aatcgaaagc gcagcagaag agtaccggcg agatgctgaa
      481 agccgcattc agcgagcacg agcagtctgt cagagcggaa ctgaacgcaa gcgcgaggag
      541 aatcagcgac gccatcagcg cccacgagca gagtatgagc gaggcgatgg agaaaaaccg
      601 gcggagcgtg ttgctgacgg ccgggaggac atggctcacc atcctgatgg tgtcagcgct
      661 gctgatcgcc acgagcggga gcattctgtg gtggcaggga cggcagataa ccgacaatta
      721 cacacatctt cgtcagcagg aagataccct ggcgaaaatg accgcccgga cgtggggagt
      781 acgctatcag gagagcaacg acggccggag gttccttatc ctgccgccgg ggatgcaagc
      841 cgaagccatc ccctacgacg ggacgacctg gatccgcctg aaacaggagt agcggaaaat
      901 gacagagctg gaaaagcagt tgctgagcgc attagagcag ctacagcagg actactcgca
      961 aaggctggac gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg gacttatgca
     1021 acgggagaac gcggtgctga acgagcgcgt gtcggacttg agcacgcagg tgctgagttt
     1081 aagcgagcag ctgcgccgct tgtccgggaa ctgaatgcca tcgaggagaa ggctgcactg
     1141 gagtatcagc gtcagaacga gctggagctg acgctccgac agaagcagaa gagtttcgat
     1201 ggtccgaaga tgaggtgaca gcccgcacga agaagggctc atcgcgttcc tgctaaatag
     1261 gctgactacc ggaatggtag tgacttatcc cggaggaagt gatgaaaatt ctcaacgtta
     1321 ttgaagccct gctgaactgt ttttacctgg tgatggagct gtgtgagttg ccggagaggc
     1381 atttgccggt ttttaaggcg ttcgcgctga ttttagtgtg actttacgct gcatgcatct
     1441 ggctgcatga ctatgcatca gtgtaatatc cagcggctgc gtttttggtg ttttttcgtc
     1501 atgtctgtcg ccactttctc gcgtctggca ccggaaacgc agccaacccc gtttctgcgg
     1561 tgcgggcaag gggtgttatg ttaaaagccg aggtctggcg gatacggaaa aaattatgcc
     1621 ggccgggctt atgcccgcgg caatccggcc gatacttaaa ttatccttca caaaggatgt
     1681 gctgcgatcc aggcagttga gtgttttccg gctcccggat gtgagccatt tttttgcctt
     1741 ctgatgcctg ttaatgcatg cggagctgcg ctggttcttc caagattgat agacgccagc
     1801 ttctctgctg gagccggcgg atttgccgta aatatgaaca ttttcttatt gttcagcccg
     1861 ctaaagatgg cgggtttttt acgcgtgaaa tccggagccg gacatcatgc cggcatggct
     1921 gaagagcggg ctttttcccg ccctgtcgca gtaatatata acgctacata aggagtagct
     1981 gataaccatg gcagtactta cattatccgg gcaatctgat tcatatgttt tcacttaaaa
     2041 atatgttctc cctgaacctc cagcgatcag ccatttcgta tgacgcaatt tgtcagaata
     2101 agcctgtcat tgagttcagt ccggaggggg tgataaataa agccagcccg ctttttctct
     2161 ccactatggg gtacagggct gatgaaataa tcggtcacca ccatcgtata ttctgcccgc
     2221 cctcgctggt aagttcaccg gaatacagcc agttctggca gcgccttgcc aggggagaaa
     2281 gctttagtgg caagtacctg cggctggcaa agggaaatcg ttccgtatgg cttgaagcca
     2341 gttatatccc cgtttctgac aggcgcggcc gtgttatcag agtcattaaa attgctgccg
     2401 atatttctga gcgcgtgcat tctgctcttg agcaggaagc agtcgtaaat gccataaacc
     2461 gttctatggc catcatcacc ttcaatcctg aagggatcgt gcttgaagca aatgaaaatt
     2521 tcgtcaatgc cactggctat aagcgggatg aaatcatagg taagcatcat cgtctgttct
     2581 gtgccgagac gctttacaaa agtgatgaat accgacattt ctgggagagt ctgaatcaag
     2641 gtgagttttt ttctggtctg tttccacgcc ttaaccgtca gggagaccct ctgtggttcc
     2701 gtgcaactta taaccctgtc tttaacagtg atgggcagct ttataaaatt gtgaaattcg
     2761 ctacagatgt tacggctgat gtcctgcgaa accagagaga gcaagaggct gctgtgcatg
     2821 catgggatat ggccgtgcag acccgagaaa gtgcgcagaa cggtgccaat gttattgaaa
     2881 atagcatcct tatgattgac agaattgcgc aggggatggg ggctgtctcc accgatatct
     2941 cacggctcaa caatcagtct gaaagtattg acgatatggt ggaaaccatc cggaagtttg
     3001 ccatgcagac aagacttatt gcactgaatg ccgcgattga agcagcaagg gctggcgcat
     3061 ccggaagaag ttttgcggtt gttgccgcag aagtgcgtaa tcttgcggcg agcgtcagta
     3121 gcgcgactga agaaattgag caagtcgtgg ccagcaatag tcaactggcc aaggatgttc
     3181 tctgtggcat tgaaaacagc ctgatgaaca ctcgggaagg ggttacactc atgcgcgaag
     3241 cgggtgaagt tatgaccagc attcagagga atgctgcagg ggttgagact gcggttaagg
     3301 atgtcgccat ttcagttaaa gccggatagg ccatggtgca tatccccata tctgtcgaac
     3361 atcacagatg atcttcttga gatcctttcc gttgcgtcgt aatctcttgc tctgaaaacg
     3421 aaaaaaccac ccggcaaggt ggctttttcg aaggttcgca agagtagacg ctcttttgaa
     3481 ccgcggtaac tggcttggag gagcacagtc accaaaactg tcctttcagt gtagccttat
     3541 cgcacccgaa acttcaagac tctcctcctc taaacctgtt accagtggct gctgccagtg
     3601 gcgctttgtc gtgtttttcc ggattggact caagttgata gttaccggat aaggcgcagc
     3661 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcccgg
     3721 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     3781 aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa cgcctggtat
     3841 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     3901 tcaggggggc ggagcctatg gaaaaacggc ttcgccgcgg ccttatcgct tccctgttaa
     3961 gcttctttct ggcatctccc aggaaatttc cgccccgcct gtaagccggt accgctcgcc
     4021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaatat atcctgtatc
     4081 gcatattctg ctgacgctcc gctgctgcat ttttctcctg ccacatgaag cacttcactg
     4141 atattcgcat ccgtgccaac atagtaagcc agtatacact ccgctagcgc tacgtgactg
     4201 gttcagggct gcgccccgaa acccgctaac agccactgac gcgcctgcgg cttgtccaac
     4261 cccgcgccga ccgggaaagg ttttcccgcc cgtcccgggg ttatcaggag actgttttgt
     4321 cagagaaacg gaacaagatg ctcaccatgt gggtgacgga ggatgagcac aggcggctac
     4381 ttgagcgctg cgacggcagg cagctggcgg cgtggatgcg gcagacctgc ctggacgaaa
     4441 agccggctcg taccgcaaaa cttccttcga tctcgccggc gctgctgcgc cagcttgccg
     4501 gtatgggcaa caacctgaac cagatagccc gccgtgttaa cgccggcgtc ggtaccggtc
     4561 atgaccgggt gcagattgtg gcggcgctga tggccatcga tgccgggctt gagcggctgc
     4621 ggcacgccgt tcttgagaaa ggaacggacg atgatcgtta aatttcatcc acgcggtcgc
     4681 ggcggtggcg ccgggccggt ggactatctg ctgggtaaag accggcagcg tgaaggcgcc
     4741 agcgtcctgc aggggaagcc ggaagaagtc cgggagctga tcgacgcgtc gccttacgtg
     4801 aagaaataca cctccggcgt cctgtcgttt gcggaggccg atctgcctcc cggccagcgg
     4861 gaaaaactga tggccagctt cgagcgggtt ctgatgcccg gacttgataa agatcagtac
     4921 agcattctgt gggtggag
//
