LOCUS       NZ_CP099322             3428 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHB45-SO-C02 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP099322
VERSION     NZ_CP099322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488699
            Assembly: GCF_029719585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB45-SO-C02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029719585.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:27:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,795
            CDSs (total)                      :: 4,677
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 34 of 64
            Pseudo Genes (internal stop)      :: 11 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            Pseudo Genes (short protein)      :: 1 of 64
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHB45-SO-C02"
                     /isolation_source="Pooled soil sample collected from floor
                     of poultry farm"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            386..649
                     /locus_tag="NFL35_RS23930"
                     /old_locus_tag="NFL35_23910"
     CDS             386..649
                     /locus_tag="NFL35_RS23930"
                     /old_locus_tag="NFL35_23910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_138627904.1"
                     /translation="MPLEQAAFLFSGVGGMLLTEMEISNTRNITLIARRLAWINAKRTH
                     IDIPSFAELLFSGQKALFFEQYNYFHSRRYTTAGTSKPPDLP"
                     /name="hypothetical protein"
     gene            841..1029
                     /locus_tag="NFL35_RS23935"
                     /old_locus_tag="NFL35_23915"
     CDS             841..1029
                     /locus_tag="NFL35_RS23935"
                     /old_locus_tag="NFL35_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024157493.1"
                     /translation="MNRQESAVLNIAKFIRAQTLLLLERIDLLDFDDEATDCEQLHEQA
                     EALYRKLSARLESDITA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(<1594..1689)
                     /locus_tag="NFL35_RS23940"
                     /old_locus_tag="NFL35_23920"
                     /pseudo=""
     CDS             complement(<1594..1689)
                     /locus_tag="NFL35_RS23940"
                     /old_locus_tag="NFL35_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            2578..3275
                     /locus_tag="NFL35_RS23945"
                     /old_locus_tag="NFL35_23925"
     CDS             join(2578..2827,2827..3275)
                     /locus_tag="NFL35_RS23945"
                     /old_locus_tag="NFL35_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_094106546.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          complement(1796..1880)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="100.0"
                     /evalue="1.43e-25"
ORIGIN
        1 ttgaaaatta tcataaacat cagataaaac gaattgcaaa ggttaaaaaa ataccgccag
       61 ttataataat tgtgatatga attacagtgt tatgaaaccc atctctggct aaatcgccgc
      121 tctattcccg ttcccgttca cctgtaaagg gcatgcaggt ctctcaatac gtggtgtaac
      181 acatatcgcc ttcaccgaaa tgatgtgaag acttcatcat tttagcaatc acaggttgcc
      241 ctaatcactt aacgttatta ttgttctatt gaagccttaa cctaaaccca ctcacaaaac
      301 gtggattatc tggttagtga ttcgcagcaa caaagcgtcc cggttcaaaa cgaatcgtaa
      361 gacgtagttt cccttgatgt agggcttgcc gctggaacag gcggcttttt tattctccgg
      421 cgtggggggg atgctgttga cagaaatgga aataagtaac acccggaata ttacgcttat
      481 tgcccggcgt cttgcctgga ttaatgccaa aagaactcat atcgatatac catcgtttgc
      541 agaactgctt ttctccggcc aaaaagcgct gttcttcgaa caatataatt attttcactc
      601 acgacggtac acaacggcag gcacgtcaaa gccaccggat ttaccataac atccgttgcc
      661 cgcaccgcct caggcgccct cagggcgttt ctgacggcgg tacggtaaaa caggtaacaa
      721 accacccgaa aagccgccag aaacgtgcca ggtaaatttt aacgccatgc atgactatgc
      781 accagaatga cggcatcctg ttttcacatc aaaatcatca caagacacag gaaaacacac
      841 atgaaccgcc aggaatccgc cgttctcaac atagctaaat ttatccgcgc ccagacactc
      901 ctcctgctgg aaagaattga cctgctcgac ttcgatgacg aagcgacaga ctgcgaacag
      961 ctgcatgagc aggccgaagc gctttaccgg aagctcagcg cccgactgga atcagacata
     1021 acggcttagc ctttccgtcg cctgcatcat ggcatccaca tcatcttttg agagatcttc
     1081 atcctccagt cgtcgctcaa ggatgaacgg acacggaatt tattacgggt cgtgaggcac
     1141 tcagtatgaa agaaaaatgc agacaataca gactggaact gtaagcaagc agtgacgaga
     1201 ttaaaaatca ggataaccag agacggtatt tccgagactc agagaggtaa tcaatacggt
     1261 taaatgcccc gggatataat gcactaacga cacgccagaa atgtatctgg gtcagctcat
     1321 ctgccccacc ttccctgatg tcagaaagta cctcgtcgta agtctttcct tcctcgcggg
     1381 ctacaatcct catcgtgtcc cttgcaaggc gatcagtaca ccctccgaaa atcagaacat
     1441 caataaacac gaagtccctt ccggtaagaa aaaaaaccgg cacgggagcc ggtaatactg
     1501 tgaaaaacag cccgctttag catcgcggtt aatagtgaca tgttatgcca ttgagcagag
     1561 atataagcac ccaccaaaac taggaggaac agacatagca gacgggcgca cccatgcaga
     1621 aagctgtgta cccccgcagc gttccagcag tagccggtac tcatcctccg tcacccacat
     1681 ggtgagtatc ttgttccgtt tctcggacaa accagtctcc tgataacgcc gtgacgggcg
     1741 ggaaaccctt tcccggtcgg cgcggggttg gacaagccgc aggcgcgtca ggcttttggc
     1801 gggtttcggg gcgcagccct gaaccagtca catagcgcta gcggagtgta tactggctta
     1861 tctattttaa cactgtgcgg aaattgacag aaatcgggct atgtggcaga ggaaaatgcg
     1921 gcttcgaagc ataagcagaa tgatgggcgg acggtgaaaa tgacgcctcc tcgctcattc
     1981 gctcactacg ttcggtcatg aagctgtggc gggcgttgcg gtgatctgac tgagtgataa
     2041 tgatttttct ggctctagat gtacaaaagc cccgtgacag aatcacaggg cttgagtact
     2101 ctaattcgtg cagatacctg ctcggatcag accgcaatca aatcctagca gggctggcta
     2161 ggaatgtcac taccagctta ataccattca acgacattaa acccatatct gcaccttgac
     2221 tctcaggtct acgccccgga ctccggcagg ataaacaggc aagcggtggg aacctgacca
     2281 gctcgggaaa gaagctggct ctacccggtc tcacgttaac caataacgta cctgataaag
     2341 tgagagtgaa ggcagcggtt gcggtgaagc gtgattgcca aggtctggcg caacacccac
     2401 aaaatcgaag cagcatacga acggcctgat gctggtcgct tcgctccccg ttttctggtg
     2461 tacccaccac gggggcatta ggctttcttt gccctaatat gggtttgcga ctctcaaatc
     2521 cgggtaatgc tgccaactta ctgatttagt gtatgatggt gattttaagg tgcttgcgtg
     2581 gcttccattt ccatcagatg tccttcctgc tccgctactg aaggcgtggt gcgtaacggc
     2641 aaaagcactg ccggacatca gcgctatctc tgctctcatt gccgtaaaac atggcaacta
     2701 cagttcactt acaccgcctc tcagcccggt acgcaccaga aaatcattga tatggccatg
     2761 aatggcgtcg gatgtcgcgc cagtgcacgc attatgggcg ttggcctcaa cacggtttta
     2821 cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacaaccg ggcagtgatg
     2881 tgattgtctg cgctgaaatg gacgaacagt ggggctacgt cggtgctaaa tcacgtcagc
     2941 gctggctgtt ttacgcgtat gacaggatac ggaggacggt tgtggcgcac gtcttcggtg
     3001 aacgcactct ggccacactg gagcgtcttc tgagcctgct gtcggccttt gaggtcgtgg
     3061 tatggatgac ggatggctgg ccgctgtatg aatcacgcct gaagggaaag ctgcacgtta
     3121 tcagcaagcg atatacgcag cgcattgagc gacataacct gaatctgaga caacatctgg
     3181 caaggctggg acggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca
     3241 tcgggcatta tctgaacata aaacactatc agtaagttgg agtcattacc catcacgatg
     3301 actaaaaaac aagcaatttg agtaaacaaa tatacgcctg acatatttgt ttactcatga
     3361 aataatatag ccacaataat aactggtgta ttttttttgg ctcccggtga tgtcaggggg
     3421 ccaacttt
//
