LOCUS       NZ_CP099321             4096 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHB45-SO-C02 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP099321
VERSION     NZ_CP099321.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488699
            Assembly: GCF_029719585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB45-SO-C02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029719585.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:27:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,795
            CDSs (total)                      :: 4,677
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 31 of 64
            Pseudo Genes (incomplete)         :: 34 of 64
            Pseudo Genes (internal stop)      :: 11 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            Pseudo Genes (short protein)      :: 1 of 64
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099321.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHB45-SO-C02"
                     /isolation_source="Pooled soil sample collected from floor
                     of poultry farm"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(188..397)
                     /locus_tag="NFL35_RS23895"
                     /old_locus_tag="NFL35_23875"
     CDS             complement(188..397)
                     /locus_tag="NFL35_RS23895"
                     /old_locus_tag="NFL35_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(401..886)
                     /locus_tag="NFL35_RS23900"
                     /old_locus_tag="NFL35_23880"
     CDS             complement(401..886)
                     /locus_tag="NFL35_RS23900"
                     /old_locus_tag="NFL35_23880"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<921..1625)
                     /locus_tag="NFL35_RS23995"
                     /pseudo=""
     CDS             complement(<921..1625)
                     /locus_tag="NFL35_RS23995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1564..1887)
                     /locus_tag="NFL35_RS23910"
                     /old_locus_tag="NFL35_23890"
     CDS             complement(1564..1887)
                     /locus_tag="NFL35_RS23910"
                     /old_locus_tag="NFL35_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2526..2684
                     /locus_tag="NFL35_RS24000"
     CDS             2526..2684
                     /locus_tag="NFL35_RS24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(2921..3190)
                     /locus_tag="NFL35_RS23915"
                     /old_locus_tag="NFL35_23895"
     CDS             complement(2921..3190)
                     /locus_tag="NFL35_RS23915"
                     /old_locus_tag="NFL35_23895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            3272..3505
                     /locus_tag="NFL35_RS23920"
                     /old_locus_tag="NFL35_23900"
     CDS             3272..3505
                     /locus_tag="NFL35_RS23920"
                     /old_locus_tag="NFL35_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            3527..3754
                     /locus_tag="NFL35_RS23925"
                     /old_locus_tag="NFL35_23905"
     CDS             3527..3754
                     /locus_tag="NFL35_RS23925"
                     /old_locus_tag="NFL35_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(1996..2074)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          2150..2638
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(79..1574)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atagccatgc agccgggtga atggcaccgg gtttcggcgc agatcctcaa tgaaagcccc
       61 gttttatccg gttatcacag cgacggaccg tcatactcct gctgacgctg cgatcgttcc
      121 agctccagcg ctttctggtg ctgcgctgcg cgctcctgct cccggtgtgc ctcgatgtcg
      181 ttcagcctta cgacaaccgg cgcagctgct cgcttaaact cagcacctgc gtgctcaagt
      241 ccgtgacgcg ctcgctcagc gctgcgttct cccgttgcat aagaccacac atcgtccgcc
      301 attccgcgaa ggcgctctcc cactcgtcca gccttttcga gtagtcctgc tgtagctgct
      361 ctaatgcgct cagcaactgt ttttccagtt ccgtcatgtg ctatttcccc gccagtatca
      421 tccagctcga atcctctccg aaccgtcccg cgtccgggtt caccctcacg cagcggcgtc
      481 gttcctctcc gcaggtcgtg agctgcacgc cgctgttcct ctcggacagc atggcctgcg
      541 tgctcttctg ctcccggatg gtcgtgtaat tgtcgagtat ctgctgcccc tgccaccata
      601 gaatgcccgc acttgaggct atcagcagca cggagaccag gacgatggtc agccacgtct
      661 ggctgaccat gcgcagcatc cctttcgtac gctggctcat ggctgaggac agcatccggt
      721 cgtggtcgtg gatggcggcg ctgattctct tctcgctttc gttcagttcc gctctgacag
      781 acttctcgtg ctcgctgaat gcggctttca gcatctcgcc ggtagtctgc tgctgcactt
      841 tcgatttctg ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctcccttc
      901 agtcggatgt ttcgcccccc gtccgggtcg gcaatgctga tgctgctttt tgtggtgcga
      961 accacctcaa aacctgcgct ttccagcgcc tcagtgacgt cctgacgcgt tttcagctcc
     1021 ccggacgagg ctaaggccag taaaccccgc gtaatcgcct gagcggcttc ctgcttcgtt
     1081 tccggcagcg cggacggcgt gaccagtgcc cgccggtttt ccggcgcgtt cgggtcgtgc
     1141 agccccagac tgccgttcac gatggtctgc caggcatcaa tgcgaggacg gtcggcgcgg
     1201 tcatagtatg gctggagccg tttgccggtc agcagctccg tgttcgggat caggaagttc
     1261 agctccagcc gccccttgtc ccggtgctca acccacagca cgctgtactg gtctttatcg
     1321 agtccgggca tcagaacccg ctcgaagctc gccatcagcg tttcacgctg cccgggcggt
     1381 aagtcctgtt cggcaaagga cagcaccccg gaggtgtact ttttggcgta gggcgaggcg
     1441 tcgataagct cccggacttc ctccggcttc ccctgaagaa cgctggcacc gtcgcgctgg
     1501 cggtctttcc ccagcagata atcaaccggg ccggcaccgc ctccgcgtcc ccggggatga
     1561 aacttaacga tcatcgtcag cccccttttc cagtacggca tgccgcagcc gctcgagtcc
     1621 ggcatcgatg gccatcagcg cggcgacgac ctgcacgcgg tcgagaccgc tactgccacc
     1681 ggcgttaacc tggcgggcaa tctggttgag gttgttcccc atgccggcaa gctgacgaag
     1741 cagcgccggc gagatcgagg gaagtttgcc ggcccgggcg ggcttctcgt ccaggcacgt
     1801 ctgccgcatc caggccgcga gctgtttacc gtcgcagcgt tccagcagac ggcggtgctc
     1861 atcttctgtt acccacatcg tgagcatttt gctgcgtttg tctgccaatc cggcctcctg
     1921 ataacgccgg gacgggcggg aaatcttttc ccggtcggcg cggtgttggg caagccgcag
     1981 gcgcgtcagt ggcatttagc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta
     2041 gcggagtgta tactggctta ctatggtggc acgacgggaa tcgggatgaa atagtcagtc
     2101 atgaaaactg tgagatgagg ctcaggtgcg taagaggctg atgcctgtcc cggtggatat
     2161 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg agcggtacgg
     2221 actcacggat aaagacgtgg cggggaaata cgtgaatgca ggaggggaag aaaggatgcg
     2281 ccgctgccgg ttttccatag gctccgcccc cctgacaagc atcacagaat ctgacgctca
     2341 aatcagtggt ggcgaaaccc gatgcggctt aaagatacca ggcgtttccc cctgatggct
     2401 ccctcgtgct cccccgcatt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     2461 cgcggtttct cattccacgc ctgacactca gttccgcgaa ggcagttcgc tccaggattg
     2521 actgtatgca cgaacccccc gttcagtccg actgctgcgc cttatccggt aactatcgtc
     2581 ttgagtccaa cccggaaaga cacgaaaaat cgctactggc agcagccatt agtaactgga
     2641 ggtatacgag agacaaatta agaggtcttg aagtatgggc ctgagagagg ctacactgaa
     2701 agaacagttt tggtttctgc ggtctcgtat acccagttac ctggttaagc agttccccaa
     2761 ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgttttc agagcaagag
     2821 attacgactg taacgacagg atcacaagaa gatcatttac tcagagagac taactaccac
     2881 tatgtttgcc tatcaacata cttaggaaac attacttgag ttaacgactc caccttactt
     2941 ttagtatctc gcaatgaatc ttccgaccgc cgttgtgttg aagttctatt gcgccaccgg
     3001 ggtataaatc actttcgttt aactcaatta aaagctcgtt cttgtgagca tcctctaaat
     3061 aaaggtaata acacccacca taaaggttct gcgtatctgg cacggcaaca aaagatttct
     3121 ttccgaacag taaaacagcc tctttttctc catgctctgt cagcaataat cgaaattctt
     3181 gtccattcaa tcctcttttc gcatgaaaaa acgacctata cttagatggt tagtgaccat
     3241 atccacttta cctttaagca gggaacctat tatgaataaa aaaacgctgt tggcggccat
     3301 gatggtcgcg ttggtgacct ttgccgctgc tggatgttct tcaaaccaag aaataaaaac
     3361 cacagatgat cgcactatcg ttacagacgg caaacccaaa gttgacagtg atacgggatt
     3421 agtatcctac aaagatgccc atacaggccg aaccgaacag atcaaccgtg acaaaattac
     3481 caacatgagt gaactagaca actgatacgg ataaaaggag ctggctttga aaaagatttt
     3541 tgtttcagtg ttcgcagcgg cagttgcgct ttcagcccta accggctgca cgcgcacaag
     3601 ctatgccatc cacaccaacg acgggcgtac aattgtcagc gacggcaagc ctacagaatc
     3661 agactcaggc ctgctgggct ataaagacgc aaatggtgtc aaacagcaga tcaataaagc
     3721 cgacgttaaa gaagtctctg aaattccgca ttgaggcatc gctctctccg aaaagggccg
     3781 ctaatgcggc ccttttcttt atctgcccca acaacaggca aaaaaagccc ccgctggggg
     3841 ccaaatacct gcagtggctg gatagtactg cattcctgat gaaggagacg aataagtatg
     3901 ggccggattt ttgcgtctgc aggctcggtc gccacaattt ttccgtaccc gccaggcatc
     3961 atgcttttac cataacatcc cttgcccgca cctctccgga cgggttcagc gcgaatgcgg
     4021 cgtcagtcct gcaaaaccga cagtaaacca ccccgcaaac cgtgcattct gcgcctgata
     4081 aatctcaccc gactgc
//
