LOCUS       NZ_CP099265             1951 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella aerogenes strain RHB08-SO-C04 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP099265
VERSION     NZ_CP099265.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488518
            Assembly: GCF_029717585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB08-SO-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029717585.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:16:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,399
            CDSs (total)                      :: 5,277
            Genes (coding)                    :: 5,147
            CDSs (with protein)               :: 5,147
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 86 of 130
            Pseudo Genes (internal stop)      :: 22 of 130
            Pseudo Genes (multiple problems)  :: 25 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099265.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="RHB08-SO-C04"
                     /isolation_source="Pooled soil sample collected from floor
                     of cattle farm"
                     /db_xref="taxon:548"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(386..1360)
                     /locus_tag="NFJ54_RS26975"
                     /old_locus_tag="NFJ54_26935"
     CDS             complement(386..1360)
                     /locus_tag="NFJ54_RS26975"
                     /old_locus_tag="NFJ54_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_279281589.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILCCVALLNPTIDNPTREQ
                     RTVKIGYQEYARMMGLSFDNAYHRLNKATSELMTRTVEIIYPEGDIEKTIFQWVNLAKF
                     SRKNQSLELVFSEDILPYLFQLKKFIKYNLEHVKAFENKYSMRVYEWLLKELTQRKTHK
                     ANIEIGITEFKFMLMLENKYSALNDFTKRILKPVTNDLNTYSNMKLAIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEAAPTPEKTHSNAHRSHEKKPNDRLIDGVRKTLLQASEAKIQ
                     LTGFEAKFLADMQEKYNLNGSFSWLTAKQEMTLEKILSKYGNI"
                     /name="replication initiation protein"
ORIGIN
        1 gattttctcg ttaaaaacga taattcgtgc aggtaaaaaa attgcgtcgt ttgtagagcg
       61 ttttaaggcg atctttgagt taccagcctc gtttgtgacg ttcttcgttg agtaattcaa
      121 taaaaactgt ttcaaattca acgtcttttg gaatatctaa ttcgcagcct ttttcgttcg
      181 ataatttcag ccattttaca ctatgagatc cctattaatt ttaatagggc gcacttagac
      241 gtttcttcac ttcgttcaga aacggtgcgc gcttgcagcg ggcgaaaata gcatgtcatc
      301 ttatcatctc aacgttaaaa ccggtgggaa aggcaagcct gcttcccata ccgatcatat
      361 tgcgcctgat ggaaaatttg catgatcaaa tattgccgta tttcgataag attttttcta
      421 gcgtcatttc ttgtttcgca gtcagccacg aaaacgagcc gtttaaattg tatttttcct
      481 gcatatccgc taaaaacttt gcctcaaaac cggtaagctg tattttcgcc tcagacgctt
      541 gtaggagcgt ttttcttact ccgtcaatca atctatcgtt tggcttcttt tcgtggcttc
      601 tgtgagcgtt tgagtgcgtt ttctctggtg ttggcgctgc ttctttggct aactctgtca
      661 ccaagtcaat ttgtttatcc agttcaactt gaaagatcag cgtgtcagca ggacgaccac
      721 gcttatcaat ggctaacttc atgttgctgt aagtgttcaa gtcatttgta actggtttta
      781 ggatccgttt tgtaaaatca tttaatgctg agtatttatt ttcaagcatc agcatgaatt
      841 taaattcagt gataccaatc tcaatgttcg ctttatgggt tttgcgttga gtgagttcct
      901 ttaataacca ttcatagaca cgcatagaat acttgttttc aaaagctttt acgtgctcca
      961 ggttatattt aatgaatttc tttaattgga acaaataagg caatatatct tcactaaaaa
     1021 ccaattctag actctggttt ttacgactaa acttagccaa gtttacccac tggaatatgg
     1081 ttttttctat atcgccttct ggataaatga tctcaacagt tctagtcatt agttcgctag
     1141 ttgctttatt caaacgatga taagcattat caaatgataa tcccatcatt cgagcgtatt
     1201 cctgatagcc tatttttacc gtcctttgct ctctagtagg gttatcaatt gttggattaa
     1261 gcaacgccac acaacacaag attaattttg tttcgtgttc tgttaaatca taacgactaa
     1321 ctgccaattc attcgctttg aaaaccacaa gttcagacat aaatcaccat tgggactaag
     1381 ttaaaacctg tccacaaatg tataacaggg acattataat taaaagtccc atttttggct
     1441 tttgttgtga ataccatgat tttttgtccc tatttcccct aatttccatg attttttgtc
     1501 cctatttcat gattttttgt ccctttttgt atctgtaaca tattgatttt atttgaatta
     1561 aaaaacctta gaataaaaaa agaataaaaa gataaaaaag aataaacccc agccctgtgg
     1621 ataactcaac catttcgcca tgatttacag tcttttttgg atgaattcaa accctcgctg
     1681 aacattcctt ttgtcttcga ccgtccagct atcgagtcgc tttgcgtttc gtgcccgttg
     1741 ggttcgctac gctcacggct tgacaggaaa gggggggtta gggggctatg ccagtccccc
     1801 cttgtatcgg caaatcaccg acagcgtttt caagggcatt tgtgcggttg ccttccatgc
     1861 cctaacgggc atcaaaccgt tcatatttga aaaaggcaac tcaaaaatac ctcgagatac
     1921 cctctagaac gtcctgtaat ccattctgag c
//
