LOCUS       NZ_CP099264             4215 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella aerogenes strain RHB08-SO-C04 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP099264
VERSION     NZ_CP099264.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488518
            Assembly: GCF_029717585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB08-SO-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029717585.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:16:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,399
            CDSs (total)                      :: 5,277
            Genes (coding)                    :: 5,147
            CDSs (with protein)               :: 5,147
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 86 of 130
            Pseudo Genes (internal stop)      :: 22 of 130
            Pseudo Genes (multiple problems)  :: 25 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099264.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="RHB08-SO-C04"
                     /isolation_source="Pooled soil sample collected from floor
                     of cattle farm"
                     /db_xref="taxon:548"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4107..4215,1..479)
                     /locus_tag="NFJ54_RS26945"
                     /old_locus_tag="NFJ54_26905"
     CDS             join(4107..4215,1..479)
                     /locus_tag="NFJ54_RS26945"
                     /old_locus_tag="NFJ54_26905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019842775.1"
                     /translation="MIRKIFFASAIVVAISGCTAMDKLAGMGVVSQQTSTFDNSTVIEV
                     SPNSLYDPNSTWGTPLQLGAIWSSAYPEEAGLILSYNSNVSSGNAAYLGLTGIDINVDG
                     KISSYKAQQQTNLSSSSYNSVSKTIYTSSKNTIVIPYKLLESMVSAKNCRIRIHTSQGN
                     QDIDFSAQSIPGGKATAIVSIKEFLAKVERFK"
                     /name="hypothetical protein"
     gene            489..986
                     /locus_tag="NFJ54_RS26950"
                     /old_locus_tag="NFJ54_26910"
     CDS             489..986
                     /locus_tag="NFJ54_RS26950"
                     /old_locus_tag="NFJ54_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_219830950.1"
                     /translation="MKKQILALALLSLPLIGMAKDSGYGNTEWGMTPNQVVAAQKGKAH
                     LIPPQKYKDQWGKVSIEDVNIGSGIYTVTFIFDASDKLVQTNVASKEKKNEGIANDQFN
                     ALNQLLTQKYGKPQFVSDEKATWKTDATTIELNKMIISGIMAQTLVRYIPNKTVISDTS
                     NL"
                     /name="hypothetical protein"
     gene            complement(1332..1547)
                     /locus_tag="NFJ54_RS26955"
                     /old_locus_tag="NFJ54_26915"
     CDS             complement(1332..1547)
                     /locus_tag="NFJ54_RS26955"
                     /old_locus_tag="NFJ54_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_063617430.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSNG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1551..2036)
                     /locus_tag="NFJ54_RS26960"
                     /old_locus_tag="NFJ54_26920"
     CDS             complement(1551..2036)
                     /locus_tag="NFJ54_RS26960"
                     /old_locus_tag="NFJ54_26920"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_279281588.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTIIREQKSTQAILSERNSGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2071..2775)
                     /locus_tag="NFJ54_RS27025"
                     /pseudo=""
     CDS             complement(<2071..2775)
                     /locus_tag="NFJ54_RS27025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2714..3037)
                     /locus_tag="NFJ54_RS26970"
                     /old_locus_tag="NFJ54_26930"
     CDS             complement(2714..3037)
                     /locus_tag="NFJ54_RS26970"
                     /old_locus_tag="NFJ54_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176142619.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAE
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3679..3792
                     /locus_tag="NFJ54_RS27030"
     CDS             3679..3792
                     /locus_tag="NFJ54_RS27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059446996.1"
                     /translation="MHEPPVQSDRCALSGNYHLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(3147..3325)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.912"
                     /coverage="83.0"
                     /evalue="7.48e-53"
     Typing          3301..3792
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.782"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          complement(1250..2724)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgataactc gactgtcata gaggtatctc caaattcgct atacgaccct aacagcactt
       61 ggggaacacc acttcagttg ggcgcgatat ggtctagtgc ctatccagaa gaggctggac
      121 taatcctttc ctacaactcg aatgtatcta gcggaaatgc cgcatactta ggtttgacgg
      181 ggattgatat aaatgttgat ggtaaaatat ccagctataa agcacagcaa caaactaatc
      241 taagcagcag ctcatataac tccgtatcca agacgatcta tacctctagc aagaatacta
      301 tcgttatccc gtataaattg ctggagagca tggtatcagc taaaaactgc cgcattcgca
      361 tacacacaag ccaaggaaat caagacatcg acttctcagc acaaagtatc cccggaggta
      421 aagcgacagc gatcgtttcc attaaagagt tcctggccaa agtcgaacgt tttaaataag
      481 gaggcgtcat gaagaagcag attcttgcac tcgctttgct atcactgcca ctgattggca
      541 tggcaaagga ctctggttat ggcaataccg aatggggtat gaccccaaat caggtcgttg
      601 cagcccagaa aggaaaagct catttaatcc cccctcaaaa gtataaagac caatggggaa
      661 aagtgagcat tgaagacgtg aatataggta gtggaatata tacagttacg tttatttttg
      721 acgcatcaga taaattagtc caaaccaacg tcgccagcaa agaaaagaaa aatgagggaa
      781 tagcgaacga ccaatttaac gcattaaatc aattacttac acagaagtat ggcaaaccac
      841 agtttgtaag tgatgaaaaa gcaacatgga aaactgatgc tacaacaatt gagctaaata
      901 agatgatcat ctccggaatt atggctcaaa cactagttcg ctacattcct aataagaccg
      961 tgatttctga cacgtctaac ctgtaataat tgtagcttca ctgagtgtct ctctcctgtt
     1021 tctatatctg aggtgggaga ttgaacataa catctcttat cggcaccgct ccgggcaggt
     1081 tcggcgtgaa tacggtgcct gcttggtaaa acccgcagca aagcgcccgg taaaccgccc
     1141 cgtttgcgtc cggtaaattt ccccggactg catagccatg cagccgggtg aatggctcag
     1201 gttttcagcg cagatacccg ccgaaagccc cgttttattc ggttatcaca gcgacgggcc
     1261 gttatacgtc cgctgccgct gcgatcgctc cagctccagc gctttctgat gctgatgcag
     1321 ctcgacggca ttcagccgtt cgacaaccgg cgcagctgct cgctcaaact ctgcacctgc
     1381 ctgctcaagc ccgtgacgcg ctcgctcagc gccgcgttct cccgttgcat aagcccagac
     1441 atcgtccgcc attccgcgaa ggcgttctcc cactcgtcca gccttttcga gtagtcctgc
     1501 tgtagctgct ctaatgcgct cagcaactgt ttttccagct ccgtcatgtg ctatttcccc
     1561 gccagtatca tccagctcga atcctctccg aaccgtcccg cttccgggtt caccctcacg
     1621 cagcggcgtc tctcttcccc gcaggtcgtg agctgaacgc cgctgttcct ctctgacagt
     1681 atggcctgcg tgctcttttg ctcccggatg atcgtgtagt tgtcgagtat ctgctgcccc
     1741 tgccaccaca gaatgcccgc gctcgaggcg atcagcagcg cggagaccag gacgatggtc
     1801 agccacgtct ggctgaccat gcgcagcatc cctttcgtgc gctggctcat ggctgaggac
     1861 agcttccggt cgtggtcgag gatggcggcg ctgattctct tctcgctttc gctcagttcc
     1921 gctctgacag acttctcgtg ctcgctgaat gcggctttca gcatctcgcc ggtagtctgc
     1981 tgctgcgctt tcgatttctg ctctaagtcc ttcgccagcg ttaaaagact gttcatagat
     2041 ggctcccttc agtcggatgt ttcgcccccc gtccgggtcg gcaatgctga tgctgctttt
     2101 cgtggtgcgc accacctcga aacctgcgtt ttccagcgcc tctgtaacgt cctgccgcgt
     2161 tttcagctcc ccggacgagg ccaaggccag taaaccccgc gttatcgcct gggcggcttc
     2221 ctgcttcgct tcaggcagcg cggaaggcgt gaccagcgct cgccggtttt ccggcgcgtt
     2281 cgggtcgtgc agccccagcc tgccgttcac tatggtctgc caggcatcaa tgcgcggacg
     2341 gtcggcgcgg tcgtaatacg gctggagccg cttgccggtc agcagctccg tattcgggat
     2401 caggaagttc agctccagcc gccccttgtc ctggtgctcg acccacagca cgctgtactg
     2461 gtctttatcg agtccgggca tcagaacccg ctcaaagctc gccatcagct tttctcgctg
     2521 cccgggcggt aaatcctttt cggcaaagga caggacgccg gatgtatact ttttggcgta
     2581 gggcgaggcg tcgataagct cccggacttc ctccggctta ccctgcagaa cgctggcgcc
     2641 gtcgcgctgc cggtctttcc ccagcaggta atccaccgga ccggcgccgc cgccacgccc
     2701 tctggggtga aatttaacga tcatcctctg cccccttttc cagcacggcg tgccgcagcc
     2761 gctcgagtcc ggcatcgatg gccatcagcg cggcgacaac ctgcacgcgg tcgtgcccgc
     2821 taccgccacc gccgttaacc tgccgggcaa tctggttaag gttgttcccc atgccggcaa
     2881 gctgacgaag cagcgccggc gagagcgagg gaagtttgcc ggctctggcg ggcttctcgt
     2941 ccaggcacgt ctggcgcatc caggctgcga gctgcttacc gtcgcaacgc tccagcagac
     3001 gccggtgctc gtcttccgtt acccacatgg tgagcatttt gctgcgttta tctgccaatc
     3061 cggcctcctg ataacgccgg gacgggcggg aaattctttc ccggacggcg cggtgttgga
     3121 caagccgcag gcgcgtcagt ggcttttagc gggtttcggg gcgcagccct gaaccagtca
     3181 cggagcgcta gcggagtgta tactggctaa atatgttaga tcagagtggc aattcaggga
     3241 agttattcat gttgcaaggg aaaatgcggc tatagggcgt aagtgcaaaa tgcaggtaac
     3301 ggtgacatga cgcctcctcg ctcactcggt cgctgcgctc ctgccgtgag actgcggcgg
     3361 gcgttacctg ctcacgaatg acaagataaa gagatggcac tcaagtcagg aaaggccgga
     3421 aaaaggatac ggcgctgccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     3481 tctgacgctc aaatcagtgg cggcgaaacc cgacaggact ataaagatcc caagagtttc
     3541 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     3601 cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     3661 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttgtcc
     3721 ggtaactatc atcttgagtc caacccggaa agacacgaca aagcgccact ggcagcagcc
     3781 actggtaact gatttagagg agttagtctt gaagttatgc gccggttaag gctaaactga
     3841 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     3901 agctctgaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttc gtggtcaaga
     3961 gattacgact ctcgcttaag gatctcaaga agatctgccc gcatcctttc aaaatccttt
     4021 taaaaaagaa tatcgtgtgc aaaatggttt cgggaatacg atgttttttt cagtctctgt
     4081 taaaaagaaa aaaataggat aacgatatga taaggaagat tttttttgct tctgcgatcg
     4141 tagtagcgat ctctggctgt acagcaatgg ataaattggc gggaatgggt gttgtttctc
     4201 agcaaacatc aacct
//
