LOCUS       NZ_CP099262             9294 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella aerogenes strain RHB08-SO-C04 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP099262
VERSION     NZ_CP099262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15488518
            Assembly: GCF_029717585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB08-SO-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029717585.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:16:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,399
            CDSs (total)                      :: 5,277
            Genes (coding)                    :: 5,147
            CDSs (with protein)               :: 5,147
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 86 of 130
            Pseudo Genes (internal stop)      :: 22 of 130
            Pseudo Genes (multiple problems)  :: 25 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="RHB08-SO-C04"
                     /isolation_source="Pooled soil sample collected from floor
                     of cattle farm"
                     /db_xref="taxon:548"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(7832..9294,1..502))
                     /locus_tag="NFJ54_RS26860"
                     /old_locus_tag="NFJ54_26820"
     CDS             complement(join(7832..9294,1..502))
                     /locus_tag="NFJ54_RS26860"
                     /old_locus_tag="NFJ54_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(1983..2132)
                     /locus_tag="NFJ54_RS26865"
                     /old_locus_tag="NFJ54_26825"
     CDS             complement(1983..2132)
                     /locus_tag="NFJ54_RS26865"
                     /old_locus_tag="NFJ54_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2217..2474)
                     /locus_tag="NFJ54_RS26870"
                     /old_locus_tag="NFJ54_26830"
     CDS             complement(2217..2474)
                     /locus_tag="NFJ54_RS26870"
                     /old_locus_tag="NFJ54_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(2484..4169)
                     /locus_tag="NFJ54_RS26875"
                     /old_locus_tag="NFJ54_26835"
     CDS             complement(2484..4169)
                     /locus_tag="NFJ54_RS26875"
                     /old_locus_tag="NFJ54_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4572..4991
                     /locus_tag="NFJ54_RS26880"
                     /old_locus_tag="NFJ54_26840"
     CDS             4572..4991
                     /locus_tag="NFJ54_RS26880"
                     /old_locus_tag="NFJ54_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5297..5560
                     /locus_tag="NFJ54_RS26885"
                     /old_locus_tag="NFJ54_26845"
     CDS             5297..5560
                     /locus_tag="NFJ54_RS26885"
                     /old_locus_tag="NFJ54_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            5550..5849
                     /locus_tag="NFJ54_RS26890"
                     /old_locus_tag="NFJ54_26850"
     CDS             5550..5849
                     /locus_tag="NFJ54_RS26890"
                     /old_locus_tag="NFJ54_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5911..6288)
                     /locus_tag="NFJ54_RS26895"
                     /old_locus_tag="NFJ54_26855"
     CDS             complement(5911..6288)
                     /locus_tag="NFJ54_RS26895"
                     /old_locus_tag="NFJ54_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            6357..6536
                     /locus_tag="NFJ54_RS26900"
                     /old_locus_tag="NFJ54_26860"
     CDS             6357..6536
                     /locus_tag="NFJ54_RS26900"
                     /old_locus_tag="NFJ54_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6564..7094)
                     /locus_tag="NFJ54_RS26905"
                     /old_locus_tag="NFJ54_26865"
     CDS             complement(6564..7094)
                     /locus_tag="NFJ54_RS26905"
                     /old_locus_tag="NFJ54_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7101..7832)
                     /locus_tag="NFJ54_RS26910"
                     /old_locus_tag="NFJ54_26870"
     CDS             complement(7101..7832)
                     /locus_tag="NFJ54_RS26910"
                     /old_locus_tag="NFJ54_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     BGC             1..9169
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1302..1880
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7105..7832)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 acacaatccc gacagccagc ccgcccgcgc accaggcaat cccgaacagg ttagccggct
       61 gcagcaggta ataatctgcg tttgcgtaga ggtccggacg caggacggtt tctccccagc
      121 tctcgataaa cacggcgaca accagccccc ccagaagggc cagaccggcc gcgccggtca
      181 gccagtacgc gtagttccag acatgtcgac gtgccctgac catccgcccg ggcgaatcca
      241 gcgaggccag caccgaaaat ggcgctggcc gtttattatc gcgcttatag cgatcggcat
      301 actcccgtcg ctccttatga ctcagcccca caaaacgcat ccgtccggcc caaagttcag
      361 ccagaccgaa aaacaggccg ataactagga aagccccggc cagaccgtaa tactcctggt
      421 acagccagct gacccacgga taaatctgcc gggccagctc cggaatgtgc cacttccagg
      481 caagcgaatc cgtattaaac atgttctctc tccttcagac gatggtttgc cggcgtgatg
      541 tgcagccccc gctgcacaag ccgcggacat gccccaaggc atggcggcgg cggtcacgtc
      601 cgtggtgatt gcgttcagct ttcaccacgg aggccagcct ctttccgccc catgcggaaa
      661 aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc
      721 ccagtgcggt cttttccggt gttccggccc ccgtcagagt tcgccatagc gagcggctga
      781 ccacccgcaa aatcaggagc agctttgctg cttcgccagg tgcgtccagc accgtggatt
      841 tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg aatttctggc gtggccggcg
      901 tccagccgga cggagccaga aagaggggga gagaccgtgc gtcggtggcg tccagccagc
      961 gtaagcaggg gatatccccc tgaggtcagc cacgccctcg cggggatgac ctgtatttct
     1021 ccgtaaggag aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg aaaaaccgat
     1081 atgattcacc tatcctggta atttattatc agggaatata aagacttaat caggggacaa
     1141 tatgccacat cagcgcgtcc ggtgtccgtc ttgtaggtgc cggggaaatt gcgcctcctc
     1201 gctcactcgg tcgctacgct ccggccgtga gactgcggcg ggcgctgcgg acacatgcaa
     1261 agttacccac agattccgtg aataagcagg ggactaacat gtgaggcaaa acggcaaggc
     1321 cacgcaggtg gcgtttttcc ataggctccg ccctcctgac tgagttcaca taaacagacg
     1381 ctttttcggt gcatctgtgg gagccggtgg ctcaaccatg aatcagtcag gagtggcgaa
     1441 acccgacagg acttaaagat catcaccgtt tccgatgaat cgctccctct tgcgctctcc
     1501 tgttccaacc ctgccgtttc ccggatacct gttccgcctt tctcccttgc gggaagtgtg
     1561 gcgctttctc atagctcaca ctctggtatc tcggctcggt gtaggtcgtt cgctccaagc
     1621 tgggctgtaa gcaagaaccc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt
     1681 cacttgagtc caacccggaa aagcacgcaa aacgccactg gcagcagcca ttggtaactg
     1741 ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag
     1801 tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg
     1861 ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc
     1921 gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1981 ttctactgaa ccgcgatccc cgtcagttca gaggaggacg atggtgccac cgttccaccc
     2041 tgaacatccc ggatatagtt tgcctgacat gcgaccagaa gaaagcctga aaaaatcaat
     2101 ataaaaagaa aaacaacctt tacctttttc atataccttc cctgtattta aaaaccccgg
     2161 tattcatccc tgaacaccgg aagtctgtat ttcaaatcat ccttgataac ttttgcttac
     2221 cagttaccat cccggtaaac aaaggataca aagtaatcaa atttactaat atcgattaca
     2281 tttttaaagt gtggctgtaa tatcgaaacc catgattttt caacatcaaa ccaaccatta
     2341 ttaatattat cctttaaagg catccccaga ctttcaatga cagaaccatc atcaccgaag
     2401 tcttttgagt attcaccacc tttaaactct tcggttttct tatcaaacca atgaatatgt
     2461 aatttaagcc ccatatttac ctcttaaaga tattttttaa tgtttcgttt tggatccggc
     2521 cctttaacct gcttacccgt ttttggatcg aatgccccga ggtgttcgcc atcactggcg
     2581 cggtaccctt caagctcacc gtgctgggaa tcccattcat aaattttacg ctttttatca
     2641 ccataccatc gggcccgctt accaccacca ccttgtttag gggttttagg tttaccctct
     2701 ttcaactctc ccaacccctt aatatcttca gtcttgggat caggaaaata atcatggccg
     2761 taatctttag ttcccttacg aggttttttc ttttcctcat ttaatttatt ttctgcatcc
     2821 ttcgcttttt tctccttttg tttccggctc tccatcgccc cggaaagcgc agccttatcc
     2881 ctggataagg attcattcat ttcgtttaaa gatgtctggg cagcttttaa tttctcattc
     2941 tgattctgaa tatccttatc aagatagcga acctgatcct gccacccgga gccatattca
     3001 tgaggcgggt tataagtcac aaaatcatct ttatttgcct ctgcctcttt taccttttta
     3061 tcggcttcct gaacagcagc ccttgcaccg gggatcgcct tctctgtcga tgcaatgcga
     3121 ctgtttaagg tcgcaatatt tttatcttca gcatccagct cagctttcgc tttatcatac
     3181 tctcttttca gcccctcttc cgggtgagca gcgtcccatg cctgctggcg acgcttttct
     3241 tcctccagac gctgttttac ctgtgccgga gtaagaacat ccgtcaccga cacatagacc
     3301 ggtttctgtc cgctctcttt cgggaaacga ataacggcct cacgggagtt accaccggcc
     3361 gtaaaaccag ccggacgtga atcacctttt tcagcaataa tgccttttgg cggggctttc
     3421 gcggccggaa cacctttagg tacgctcacc tgcagagacg ggagacccgg aatcgacaca
     3481 ctgaataccc ccggacgttt tgtcggtttc gcatccacca caggaacact cattggccgg
     3541 ccggtgacaa ccgcaatatg ctgccgctcg tccttcacaa tatccgccac acgtttagtg
     3601 acgctgaccg tcgcctggtc cagcggcagg gagctgaccg gcgtctccgt taccgtgtct
     3661 gccggcaatg acgtcacgat ttttgacatc attttcggat catcttttgc tatctcagaa
     3721 ggcagcactc cgtaaatcgc aatcccccac agaccaaact taaacggacc tttcagggca
     3781 gccagcacat cagcaacagc ggccgacaac gcatcgccgg aaacggataa agccagtcct
     3841 tcagcccccg gagtggccag ggcaggcaga ccgaaggcca tcgcggtggc cgaagactgt
     3901 ccgccaccag agctcccgct atgattgccg ctgttaccgc cattaccacc gggtttactc
     3961 ccgccgttac caaactcccc cgggttatag ttatgaatat ccccgttagg tgtatgcgtc
     4021 gtaccccagc ccggcccgct gtttggatcc gtaccaccac ctgatgaagg gccaccttta
     4081 cctgatgtac cgttcacatt cccactggcc tgaccaccat tgtgtcccag acctgaatta
     4141 cccggacctc gtccgtctcc gccactcata tacttttcct ctttgacttt taaaacaata
     4201 aactaaaata aaacactgta catataacca ctggttttat atacagtata aaaattacgc
     4261 tgccttgttt ttcatgtcaa gtctgtaaat ttgcgttttt gtgaaaatga tcaaaaaaac
     4321 agccactcac accatgcgca atcccctcca gatgcaacaa aaaccagcac aaacggcgcc
     4381 gttctccggc ctcagtatgt tttactgaaa tcattatatg agataccaga aagccccctc
     4441 cgggacagga aaagaaacgg ggggtctggt tcgggttgtt cggtcactga acacggtcag
     4501 aaactgatga taagatagcc tgcggatata taactcaatg agaaaaaaga attaaaggta
     4561 aggagacgct catgagacgt tccggcagca aactgtgtct tatcgccatg ataacagcga
     4621 cagcaactct cactgcctgt gctccaaagg gcagcgtgga acagcatacc cggcattacg
     4681 tatatgcttc agatgacggt tttgatccca atttttccac ccagaaagct gacacaacac
     4741 gaatgatggt gccttttttc cggcagttct gggatatggg cgcaaaggat aaagcgaccg
     4801 gaaaatcacg gagtgatgtg cagcaacgca ttcagcagtt tcacagtcag gaatttttaa
     4861 actcactcca aggcacaaca caatttgcgg gtaccgatta ccgcagcaaa gacctcacac
     4921 cgaaaaaatc aaggctactg gctgacacca tttcagctgt ttatctcgat ggctatgaag
     4981 gcagacagta agtggattta ccataacgcg tcattacgcg caccgctcag acgggctcag
     5041 ggcgtttccg gcggctacgg gataaaacag gcaacaaccc ccccggaaaa ctgccggaaa
     5101 cgcgcccgat aaattttatc ctaatgcatg cctatgcacg caactgcatg gcatatgttt
     5161 tgacgtaaaa tggatgccgt aagccctttt ttaccgactt tctgcttcat cagcctgact
     5221 gacacgacca tattctttca gaccgccccc tgctaaactt ccttcatatt gccgcattaa
     5281 cccgggaggc tccgcaatga gcaacactac acactacgaa aacgccaact ttcttcggga
     5341 actggctgaa agcctgcccc gcatactgcc ggaaggtggc ccggacaaag ctgcgctgtt
     5401 gcagcgactg gctaacgaag aactggccca ggcagagtat gaagatcagg ttcgcgccaa
     5461 agtgaccgcc gcccgtgctg acacgcgtcc gggtatgacc acggaacaac tccgccagcg
     5521 cctgcatggc cgttatcagg aactgcgcga tgccgtatga cgtggaatgg aagcagggag
     5581 ccatcgagga tgtaacgacg ctgtttgact atatcgcgga aaattcatcc ctgtgggatg
     5641 cgcagaacgt taccgaccgg gttctcgctg cggcggacaa actggccgac ttcccgcgac
     5701 tctatgaagt ggactcacgc tatggcgagg acgttcgccg gatcagtctg atgggacagc
     5761 atgtcctcta tgatgttgat gaccagacca gaaaagtccg ggttctggcc gtggtcggcc
     5821 agcgccagaa tccccatatt gttctctaat aaccggtgac ggggacgcaa ttcattgcgt
     5881 ggccgtcgcc ggttggacaa gcacagcgcg tcagcgtttt ccccacggcc accaccggcg
     5941 cggtgttccg gccggggtct ctgctgtctc cggtttgtgt tccagcagtc tcatggactg
     6001 catttcctgc cgtagcccgg ccacctcctc cctgagtgct tttatctccg cgatcaacgc
     6061 gtcggaggcc attttttcag tctcacccga cagtctcaac tcgtcccgct cgtgagtctc
     6121 atcctgtctt aactcaccgt acacccggat aagctcgctg gtatccacca cccggccgcc
     6181 atcagcttcc gtacggtatg atacgcggcc tttcgccata tcccgataca gggaacttcg
     6241 ggctttaccc gtcagttccg tcgcctgtct caccgtcacc cacgccattg tctcaccccg
     6301 ctatttttga gacagtatac ctgacaggcg cacaccatgc ccggaaggaa agaatcatga
     6361 caaaacagga aaaaacagcc ctcaatatgg ctaaatttat taaggagcag tcgctgctat
     6421 tgctcgataa actgaatgag ctggatatgg ataccccggc cgacatgtgc gagcggctac
     6481 acgaggatgc ggaaacactc tggcaggtga tgcgggataa gctcggggag gaataacagc
     6541 accggcatgg ccggtgctgg catttatttt ttgccgaacg gccaccaccg gcgggcaggt
     6601 ttgtcctccg gcagttcagg taccggcacg ttaaaatagc ggctgttata ttcccgcatc
     6661 agctccgctt tcagcttctc gttcaccgcg ctttccccgt gccgctcact caccacgtca
     6721 atcatcgcgt taatctgctc cggtgagcac ttcccggcat caaccacctg cacaccgttc
     6781 tcgatggcga accgctccac cttgcgatag aggtaatcac acagcttcat tcctgtgggt
     6841 ttgttccgtt cccattgcgg atttttacca gtgaaaaagt acgtggcttt ttcgtcgctg
     6901 ttgagccgct cgtcatacat cccgatggat ttcatcatcg ccagatgctg ggcccggtcg
     6961 acaaaacaga aaacatacgc cccggtctgc caggcgagca ccaccatatc ttccagatgc
     7021 tcgctggtcg gcatgatgca gcgctcgccg ttccggtcct ccgcgtcttc aataaatacc
     7081 atgtacagcg ccataatccc tcactccgtt gtcataaatt taaagcacgc aaacaaccgt
     7141 tcacgatcca gctctgccgt tttgcgggcc tcgccggtct gtgggttcac gatttcatcc
     7201 accgacagga ttgcccgctg caccctgacc tgcgtcgccc ggtccgggca gatgtaccag
     7261 atttcatcga tgccgttagc tttgcggttg aacagatacc cggacaccac ttcctgatac
     7321 cggcgacggc tcttgacggt gcgctccacc tcaaacgcca ccgttttacc gtccagctcc
     7381 gccagggcgt ccggcacttt catgccttta agactcagac ggtgcagaat gcgccagccg
     7441 gtggcccctc tggcttccat atcaaggcgc agggattgca cggccagccg gtgcggcaga
     7501 tgtgctgccg acacccttga cggctgaaac ggaataacat ccgccagcgg ttcgtcctcg
     7561 tcgaacgaga acgccacgcc atgcggcgtc agcccccaca cgctgacctg acggccgtcc
     7621 accggatatt tggtccggac cagcagccct ttcgccacca ggctgtcgag gaagcggtag
     7681 accggctgca ccgccgcaat ccccagccgc cgcgcaacca gctccgccgt ggaacacagc
     7741 tcttcccgca accagcgcag ggtcagccgg gttttctccg cgttccgcgc ctgtcgtttc
     7801 gcgtggctga cgttgtacag ctccagcgcc atcagtaaat ccccgcaaaa tcatcatccg
     7861 ctttcggccc gccgccgtcg gccgggggcc ggaactccgc caggggatcg gttttcgcca
     7921 gcaccgggaa acgctgcaac gggatttccc ggcgctcaac ccgcagcgga ctgctgaacg
     7981 ccatccgggc cacacccagc ccggtcatga ccgcgaaccc gtccggcagg tgttgcaggg
     8041 tgttcacgtc aaacagcggc cgcgcctcct tctggatgaa gctgtccccg ctgatatgct
     8101 ccacgttccc cgcctcccgg ctggcacggc ggcgctccac gtccaccctg atttccccgg
     8161 tctgccccgc cgcccactgc gcggactcca ggctggccgc ccggtaaaac cagcggatcg
     8221 gcgtgttatc cagtaccgtg gttttcacaa attcagcggg cagatcctgc ccgcactgcc
     8281 cgaaatcgcc cagcgactgg tgcgcgagca acagattgca gtcccggctg cgcagcgtcc
     8341 ccagtgcgtt cagtacatac ttcgacagca gatatttaat ttcatcgagc atcacgctga
     8401 cgtgtggcca ctcccggaac tcgtcccgcg caatgatgat ttgcgcgcag cgggcaaaca
     8461 gcattttttg cacacggatc accgcctcgt cgtccatcga gccgataacg tacaggcagc
     8521 cgccgccgtt gataatcccc gccacatcga tgccactgtc cgtctgcagg acgctcaggt
     8581 cagcgacgtt ttccagccta gtgatcaacc ccttcacgtc ctttttcagg gcctccggca
     8641 gcgcgtacgc cgccgccagc acctgctgaa tgtttgcccc ctgcggaaac tgctccgcaa
     8701 tcagcttcgc cgctttctgc tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcgc
     8761 ccaggttaag cccggcaatc agcaggtttt tcagcgcgta ctggtcgaga tcgcggaaca
     8821 gattcagctg cgccggtttc cccggccgca gatccagcag ggtgaagtcc ggacagtgcg
     8881 cccggaagac gtgcggcgcc catgcgtccc ctttggggtc gaagtacacc accgcgtccc
     8941 cgaaatcgcg tacgcagcga atgagcgcgt tggtggccat cacgctcttg ccgctcccgg
     9001 gcaggccgag gatctgaatg ttggtcttgc gccaggtatc acgcggaacc agcaccggct
     9061 tgccctgctc atccttaccc agaaacaccg cctgctcctg ccgggcctgc tccgaggaaa
     9121 aatcggccag cgcattaacc ggtaccggct ccccgaaatg caggtggcgc acgtcggtca
     9181 tctctcctgt ccgctggctg ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct
     9241 cgtcagcagc cgcaaaatga ccgtcaagca gcaggaacgc cagcagccgc gcca
//
