LOCUS       NZ_CP098761            10060 bp    DNA     circular CON 23-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KP167 plasmid pKP167-10, complete
            sequence.
ACCESSION   NZ_CP098761
VERSION     NZ_CP098761.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28922061
            Assembly: GCF_023796915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liang,J. and Bao,Q.
  TITLE     Investigation of Class 1 Integrons in Clinical Klebsiella pneumoniae
            Complex Isolates and Characterization of a Double Multiple Mobile
            Genetic Element Sequences in an IncFIIK Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liang,J. and Bao,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2022) School of Laboratory Medicine and Life
            Science/Institute of Biomedical Informatics, Wenzhou Medical
            University, Chashan University Town, Wenzhou 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1; unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 05:01:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,562
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,292
            CDSs (with protein)               :: 5,292
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 69 of 148
            Pseudo Genes (incomplete)         :: 80 of 148
            Pseudo Genes (internal stop)      :: 39 of 148
            Pseudo Genes (multiple problems)  :: 31 of 148
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098761.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP167"
                     /isolation_source="secretions"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP167-10"
                     /geo_loc_name="China"
                     /lat_lon="27.03 N 119.39 E"
                     /collection_date="2020-01-06"
                     /collected_by="JiaLei Liang"
     gene            600..2564
                     /locus_tag="ND678_RS27735"
                     /old_locus_tag="ND678_27725"
     CDS             600..2564
                     /locus_tag="ND678_RS27735"
                     /old_locus_tag="ND678_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2564..3295
                     /gene="mobC"
                     /locus_tag="ND678_RS27740"
                     /old_locus_tag="ND678_27730"
     CDS             2564..3295
                     /gene="mobC"
                     /locus_tag="ND678_RS27740"
                     /old_locus_tag="ND678_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3302..3832
                     /locus_tag="ND678_RS27745"
                     /old_locus_tag="ND678_27735"
     CDS             3302..3832
                     /locus_tag="ND678_RS27745"
                     /old_locus_tag="ND678_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3859..4038)
                     /locus_tag="ND678_RS27750"
                     /old_locus_tag="ND678_27740"
     CDS             complement(3859..4038)
                     /locus_tag="ND678_RS27750"
                     /old_locus_tag="ND678_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4064..4492)
                     /locus_tag="ND678_RS27755"
                     /old_locus_tag="ND678_27745"
     CDS             complement(4064..4492)
                     /locus_tag="ND678_RS27755"
                     /old_locus_tag="ND678_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4656..4970)
                     /locus_tag="ND678_RS27760"
                     /old_locus_tag="ND678_27750"
     CDS             complement(4656..4970)
                     /locus_tag="ND678_RS27760"
                     /old_locus_tag="ND678_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4958..5236)
                     /locus_tag="ND678_RS27765"
                     /old_locus_tag="ND678_27755"
     CDS             complement(4958..5236)
                     /locus_tag="ND678_RS27765"
                     /old_locus_tag="ND678_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5411..5776)
                     /locus_tag="ND678_RS27770"
                     /old_locus_tag="ND678_27760"
     CDS             complement(5411..5776)
                     /locus_tag="ND678_RS27770"
                     /old_locus_tag="ND678_27760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5773..6144)
                     /gene="tolA"
                     /locus_tag="ND678_RS27775"
                     /old_locus_tag="ND678_27765"
     CDS             complement(5773..6144)
                     /gene="tolA"
                     /locus_tag="ND678_RS27775"
                     /old_locus_tag="ND678_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6418..6663)
                     /locus_tag="ND678_RS27780"
                     /old_locus_tag="ND678_27770"
     CDS             complement(6418..6663)
                     /locus_tag="ND678_RS27780"
                     /old_locus_tag="ND678_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6990..8675
                     /locus_tag="ND678_RS27785"
                     /old_locus_tag="ND678_27775"
     CDS             6990..8675
                     /locus_tag="ND678_RS27785"
                     /old_locus_tag="ND678_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8685..8942
                     /locus_tag="ND678_RS27790"
                     /old_locus_tag="ND678_27780"
     CDS             8685..8942
                     /locus_tag="ND678_RS27790"
                     /old_locus_tag="ND678_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9027..9176
                     /locus_tag="ND678_RS27795"
                     /old_locus_tag="ND678_27785"
     CDS             9027..9176
                     /locus_tag="ND678_RS27795"
                     /old_locus_tag="ND678_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1990..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9280..9858)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2565..3292
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tcaggatagg tgaaccatgt cggtttttcg ctgaacattt tacgttaacc tatctctgtt
       61 gcttttttct ccttgcggag aaatgcaagt catccccgct ggggcgtggc tgacttcagg
      121 ggaaaagacc ttgcttacgc tggctggacg ccaccgacgc acggtctttt cccctctttc
      181 tggctctgtc cggctggacg ccggccacgc cagaaattca cccggccccc gtcaggctca
      241 cgcctgacca cccacaaaaa tccacggtgc tggacgcacc tgacgaagca gcaaagctgc
      301 tcctgatttt gcgggtggtc agccgctcgc tatggcgaac tctgacgggg gccggaacac
      361 cggaaaagac cgcactgggg tgccgccttt tcctctcaca cgctggggcg tgctcgttgt
      421 tcctccgggg ctgcgccttt tccgcatggg gcggaaagag gctggcctcc gcggtgaaag
      481 ctgaacgcaa tcaccacgga cgtgaccgcc gccgccatgc cttggggcat gtccgcggct
      541 tgtgcagcgg gggctgcgca tcacgccggt aaacccgtcg tctgaaggag agagattgca
      601 tgtttaatac ggattcgctt gcctggcagt ggcatatccc ggagctggcc cggcagattt
      661 acccgtgggt cagctggctg taccgggagt attacagtct ggccggtttt tttctggtta
      721 tcggcctgtt tttcggtctg gctgaactct gggccggccg aatgcgtttt gtggggctga
      781 gccataaggc gcggcgggag tatgccgatc gctataagcg cgataataac cggactgtgc
      841 cattttcggt gctggcctcg ctggattcac ccgggcggat ggtcagggca cggcgacatg
      901 tctggaacta cgcgtactgg ctgaccggcg ctgccggtct ggcccttctg ggggggctgg
      961 ttgtcgccgt gtttatcgag agctggggag aaaccgtcct gcgcccggac ctctatgcga
     1021 acgcagatta ttacctgctg cagccggcta acctgttcgg gatagcctgg tgtgccggag
     1081 ggcttgtcgc cggcgtcctt ctggcgcggc tgctggcgtt cctgctgctt gatggtcatt
     1141 ttgcggccgc tgacgaggcg gtgaacgcga aactgaatca ggagagccgg cgcagcagcc
     1201 agcggaccgg agaaatgaca gacgtccgcc acctgcattt cggggagccg gtaccggtta
     1261 acgcgctggc cgatttttcc acggagcagg cccggaaaca gcaggcggtg tttctgggta
     1321 aggatgagca gggccagccg gtgctggtcc ctcgtgatac ctggcgcaag accaatattc
     1381 agatcctcgg cctgcccggg agcggcaaga gcgcgatggc caccaacgcg ctcattcgct
     1441 gcgtacgcga tttcggggac gctgtggtgt acttcgaccc caaaggggac gcgtgggcgc
     1501 cgcacgtctt ccgggcgcac tgcccggact tcaccctgct ggatctgcgg ccggggaaac
     1561 cggcgcagct gaatctgttc cgcgatctcg accagtacgc cctgaagaat ctgctggttg
     1621 ccgggtttaa cctgagtgag accggcgacg tggcggacca ctaccgcatc agcgagcaga
     1681 aagcggcgaa gctgattgcg gagcagttcc cgcagggggc aaacattcag caggtgctgg
     1741 cggcggcgta cgcgttgccg gaggccctga aaaaggacgt gaaggggctg atcaccaagc
     1801 tggagaacgt ggccgacctg agcgtcctgc agacggacag cggtatcgat gtggcgggga
     1861 taatcaacgg cggcggctgc ctgtacgtca tcggctcgat ggacgatgag gcggtgatcc
     1921 gggtgcagaa gatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg gatgagttcc
     1981 ggaggtggcc gcacgcgagc atcatgctcg atgagattaa atatctgctg tcgaagtacg
     2041 tgctgaacgc gctggggacg ctgcgcagcc gggactgcaa cctgctgctc gcgcaccagt
     2101 cgctgggcga cttcggtcag tgcgggcagg atctgcccgc tgattttgtg aaaaccacgg
     2161 tactggataa cacgccgatc cgctggttct atcgcgcggc cagccaggag tccgcgcagt
     2221 gggcggcggg ccagaccggg gagatccggg tggacgtgga gcgccgccgg gccagccggg
     2281 aggcggggaa cgtggagcac atcagcggcg acagcttcat ccagaaggag gcgcggccgc
     2341 tgtttgacgt gaacaccctg caacacctgc ctgacgggtt cgcggtgatg accgggctgg
     2401 gcgtggcgcg gctggcgttc agcagcccgc tgcgggttga gcgccgggaa atttcgctga
     2461 aaaccttccc ggtgctggcg aaaacggacc cgctggcgga gtacctgcct ccggccgacg
     2521 gcggcggacc aaaagcggat gatgattttg cggggcttta ctgatgacac tggagcagta
     2581 caacgtcagc cacgcgaagc ggcaggcgcg gaacgcggag aaaacacggc tgaccctgcg
     2641 ctggctgcgg gaagagctgt gttccacggc ggagctggtt gcgcgacggc tggggattgc
     2701 ggcggtacag ccggtttacc gcttcctgga cagtctggtg gcgaaagggc tgctggtccg
     2761 ggcgaaatac ccggttgacg gccgtcaggt cagcgtgtgg gggctgacgc cgcacggggt
     2821 ggcgttctcg ttcgacgagg acgagccgct gacggacgtt attccgtttc agccgtcaag
     2881 ggtgtcggcg gcgcagctgc cgcaccggct ggccgtgcag tccctgcgcc tctccatgga
     2941 gggccggggg gccaccggct ggcggtacct gcaccgtatg gccctgaaag ggatgaaagt
     3001 gccggacgcg ctggcggagc tggacggcag aacggtggca tttgaggtag agcgcaccgt
     3061 caagagccgc aggcggtatc aggaagtggt gtccgggtat ctgttcaacc gccgggcgaa
     3121 cggcatcgat gaaatctggt acatctgccc tgaccggccg acgcttgtcc gggtacagcg
     3181 ggcgattctg gcggtggatg aaatcgtgaa tccacagacc ggcgaggccc gcaaaacggt
     3241 ggagctggac cgggagaggc tgttcgcgtg ctttaaattt atgacaacgg agtgagggat
     3301 tatggcgctg tacatggtat ttattgagga cgtggaagac aaagacggcg agcgctgcat
     3361 tatgccaacc agcgagcatc tggaagacat ggtggtgctc gcctggcaga ccggggcgta
     3421 tgttttctgt tttgtcgacc gggcccagca tctggcgatg atgaaatcca tcgggatgta
     3481 tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt ttcaccggtg aaaatccgca
     3541 atgggaacgg gacaaaccca caggaatgaa gctgtgtgat tacctctatc gaaaggtgga
     3601 gcggttcgcc atcgagaacg gtgtgcaggt ggttgatgcc gggaagtgct caccggagca
     3661 gattaacgcg atgattgagg tggtcagcga gcggcacggg gaaagcgcgg tgaacgagaa
     3721 gctgaaagcg gagctgatgc gggaatataa cagccgctat tttaacgtgc cggtgcctga
     3781 actgccggag gacaaacccg cccgccggtg gtggccgttc ggcaaaaaat aacgccagca
     3841 ccggcatggc cggtgctgtt attcttcccc gagcttatcc cgcatcacct gccagagtgt
     3901 ttccgcatcc tcgtgaagcc gttcgcacat gtcggccggg gtatccatgt ccagctcgtt
     3961 cagcttttcc agcaacagca gcgactgatc cttaatgaat ttagccatat tgagggcagt
     4021 tttttcctgc ttcgtcatgt tctccattcc cttcggtaag ttctcagcta aaccagccgg
     4081 accagaagcg ttttttcttc tgtgtcagtt ctgttgtcaa tctgtccact tcggcctgga
     4141 gtttccggcg ctcatcatcg tgctggcggc gttcttcttc ccgggatgtt ttatcttcca
     4201 gcatcagggt gacagcctgc cggagatcgg ccagttcctt ttgcattgcc agaagctgtt
     4261 cctgtgggaa atttttttgt gtattttcac ggtgtgaaat gccgggaatt tgtttctcag
     4321 ggatattcac atttccgtat acacggatca gttctgacac atccacgaca gggttgtttt
     4381 tcccgtcacg tgacacggta acctttccct gttttacatg attgtatagc gttcttctgg
     4441 taatgcccgc ggcttgtgct gcctgtgaaa ggttgagcaa tgttttcgcc actgtatacc
     4501 cctgatttta tcgggtgtga aaggtgtgta atttctcacg gtaaatgtat cgctgaattt
     4561 tacccgtttt tccgttcagg atgtggtttt ctgacgcgct gcgcttgtca aaccggcgac
     4621 ggccacgcaa tgaattgcgc ccccgtcatc ggttattaga ggctgtctgc ccccggatac
     4681 ccggattctt tctgatgcct gacggtgacg atcacaatct ggtccatttc ccggtcgtgg
     4741 cggtagagca tcacatagcc actgtcgcca aacccgatca ccagttcctt atattccagc
     4801 ggcagaaacg gcaccgggcg gccaatgtct ggcaatgttt tcagttgctg gatagcccgg
     4861 acgatcacct cacctccttt tctggctgcc agccgatttt tggttttgag aaagtcctga
     4921 aggcgttgta aatcgcgctg tgccagcgcg gaaatcgtta cctgtggcat ggcggagcat
     4981 cctgctcgtt ctcagttccc caggtactta tccaggcttc cgcttcttcc gcagtaaggt
     5041 gcagacccgt ttcctgatag tgctgccatg cggcctgtcc gtcccgcagg tactggctgc
     5101 gcttctcttc ccggtcgatg tattcggtga ttgcctccag cattagcgcg tgcgctgagc
     5161 ggtgccggtc atcggccagc gttttcagcc ggtccttaag ttcttcatcg agcctgatgg
     5221 atgttgctga tgacataaaa gcccctttgt agtcatatgt attacacatg actactttaa
     5281 tccgtggcgg cgctatttgc cagaaatacg gcttttgctg cccatttttc agcttcgctt
     5341 ccactaatca gggctgctac aggctcactc atcatccagg actattgcag cctgcgggtt
     5401 aaaggacctg ttactggcgg caaccatcat cattgagctt ttgggtggtg actggtcgcg
     5461 gatcctgtaa gccgtcattt ttcagactga attcaaaatt tataacctgt cgcattgccg
     5521 ggtgccctgc gggatatagc cagtgcctca ttgccgtcag cgtttcccga aaaaaaaggt
     5581 gacagccggt aagcgacacg ttgtccactc gtccatcgtc cagaatatca aacgtggcgg
     5641 tcacctgacc ttcgatgcgt tttgctgcgg cctgtaccgg gtaggttggc tgcagtgtgg
     5701 ttacgggttt tagtggtatc gcatgtaccg gagtgaggaa catggccgcc agcactgtca
     5761 gagtaaaagc tttcatggtc tgaaatccag aggggcgttt ttgaatattt gccaggtatt
     5821 ttcatccggt gccggcggaa ttttggcgtg ggtaacggct gatattgctg ctttacagag
     5881 tttcggatct cctccttcag caatggcgct gttcagagtg ccatcacgtt gtatggatat
     5941 ccgcacggtg cacatttttc ccgcataggc ctctggttca aacatctgca tttcaacagc
     6001 ggtcttgatg gccgcagcgt agctactgat tttagatgac ttatccagag tataaatttt
     6061 cggcggctgg gtggtggaat gcttacaggc cgtcaggagt aaggggctgc acaatgccag
     6121 caggataaag ggtattaaac gcatcgggtt gatgacctgt cagtaaaaaa tgttacatgt
     6181 atatttgtca ctctttacgg ccacgttcat ttacataaac acggcttatc gtatcgtttt
     6241 tacaccaaac ggcccgccat gcagttgcgt gcataggcat gcagcgggtt acaatttacc
     6301 gggcgcgttt ccggcggttt tccgggtggt ttgttgcctg ttttatcccg tagccgccgg
     6361 aaacgccctc agaccgtctg agcggtgcgg gcaatgcgac gttatggtaa atacccctta
     6421 ccggcggcgc tgtggctgcc aggtgaaggt ggtttccgta gcctcgtatt tctcccgacg
     6481 catttcctgc gcccgcctgg cttcacgttc gtcctgaatg gcacgaagca ccctttcccg
     6541 cacctgttgt tttaccgcct ttggcagcgt tttgttccct ttcaggccca tggccagtgc
     6601 ctgagcgaca aaggcgtccg ccaggcgctt ttcttcagcg ggagtcagtt tcaggtgcgt
     6661 catagtggcc tccgggcgtc tcaggtttcc cgaacagttt aaccgcaggt cctctcctca
     6721 tgccagttag ctttcagatg tgtctatacc atctgaaatg taagtgtgat gaatgtctta
     6781 aataataaaa tttgttgcca tgagaaattt tcgggaaaag acgcgatctg cttacaggtg
     6841 gctccgggcg cttttttgtg cctgaagatg agaactaatc tcatatattg acacctgaaa
     6901 acgggaggag taaggtaata attatactgt gtatatgtac agtatgttgg ctttaattta
     6961 ttgttttaaa tgtcaaagag gaaaacggta tgagtggtgg agacggtcga ggtccgggta
     7021 attcaggtct gggacataat ggtggtcagg ccagtgggaa tgtgaacggt acgtctggta
     7081 aaggtggccc ttcatcaggt ggtggtacgg atccaaacag cgggccgggc tggggtacga
     7141 cgcatacgcc gaatggagat attcataact ataatccggg ggagtttggt aacggcggga
     7201 gtaaacccgg tggtaatggt ggtaacagcg gcaatcatag cggtagctct ggtggcggac
     7261 agtcttcggc cactgcgatg gccttcggtc tgcctgccct ggctactccg ggggctgaag
     7321 gactggcttt atcagtttcc ggcgatgcgt tgtcggccgc cgttgctgat gtgctggctg
     7381 ccctgaaggg gccgtttaag tttggtctgt gggggattgc gatctacggt gtgctgcctt
     7441 ctgagattgc aaaagatgat ccgaaaatga tgtcaaaaat tatgacgtca ttaccggccg
     7501 atgcggtgac ggagactccg gcaagtactt taccactgga ccaggcgacg gttcgtgtca
     7561 gacaacgggt tgtggatgtg gtgaaggatg agcggcagca tattgcggtt gtcgcaggtc
     7621 ggccaatgag tgtccctgtg gtggatgcga aaccgacaaa acgtccgggg gtattcagtg
     7681 tgtcgattcc gggtctcccg tctctgcagg tgagcgtacc gaaaggtgtt ccgacagcga
     7741 aagccccgcc aaaaggcatt gttgctgaaa aaggtgattc acgtccggct ggttttacag
     7801 ccggtggtaa ctcccgtgag gccgttattc gtttcccgaa agagaccgga cagaagccgg
     7861 tttatgtgtc ggtgacagat gttcttaccc cggcacaggt aaaacagcgt caggaggaag
     7921 aaaagcgtcg ccagcaggca tgggacgccg ctcatccgga agaggggctg aaaagagact
     7981 atgataaagc gaaagctgag ctggatgccg aagataaaaa tattgcgacc ttaaacagcc
     8041 gcattgcatc gacagagaag gcgctccccg gtgcaagggc tgctgttcag gaagccgata
     8101 aaaaggtaaa agaggcagag gcaaataaag atgattttgt gacttataac ccgcctcatg
     8161 aatatggctc cgggtggcag gatcaggttc gctatcttga taaggatatt cagaatcaga
     8221 atgagaaatt aaaagcggct caggcatctt taaacgcaat gaatgaatcc ttatccagag
     8281 ataaggctgc acttaccggg gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg
     8341 atgcagaaaa taagttaaat gaggaaaaga acaagcctcg caaggggact aaagactacg
     8401 gccatgatta tcatccagta cctaaaacag aagacataaa gggacttgga gagttaaagc
     8461 gaggtgatcc taaaacacct aaacaaggtg gtggtggtaa gcgggcccga tggtatggcg
     8521 ataaaaaacg taaaatttat gaatgggatt cccagcacgg tgagcttgaa gggtatcgtg
     8581 ccagtgatgg cgaacaccta ggcgcatttg atcccaaaac gggtaagcag gttaaagggc
     8641 cagatccaaa acggaacatt aaaaaatatc tttgagaggt aaatatgggg cttaaattac
     8701 gtttgacatg gtttgataag aaaactgaag agtttaaagg ggaagagtat tctaaagact
     8761 ttggtgacga tggttctgtc attgaaagct tgggaatgcc tttaaaggat aatattaata
     8821 atggtgagtt tgatttgaag aaagaatggg ttcctttact acagccattt tttaaaaatc
     8881 aaataaaacc tgatgtggat aattgttata tagcttttga ttatcgggac ggtaactggt
     8941 aacaaaaaat tatcaaggat gatttgaaat actgacttcc ggtgttcagg gatgaatgcc
     9001 ggagtcttta aatacaggga aagtatatga aaaaggtaaa gactattttt ctttttatat
     9061 tgattgttgc tggctttctt ctggccgcat gtcaggcaaa ttatatccgg gatgttcagg
     9121 gcggaacggt ggcaccatcg tcctcctctg aactgacggg gatcgcggtt cagtagaaaa
     9181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga
     9241 aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacc
     9301 gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccggact
     9361 ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg cgaactccca
     9421 gttaccaatg gctgctgcca gtggcgtttt gcgtgctttc ccgggttgga ctcaagtgaa
     9481 cagttaccgg ataaggcgca gcagtcgggc tgaacgggga gttcttgctt acagcccagc
     9541 ttggagcgaa cgacctacac cgagccgaga taccagtgtg tgagctatga gaaagcgcca
     9601 cacttcccgc aagggagaac ggcggacagg tatccggtaa acggcagggt cggaacagga
     9661 gagcgcaaga gggagcgacc taccggaaac ggtggggatc tttaagtcct gtcgggtttc
     9721 gcccgtactg tcagattcat ggttgagcct cacggctccc acagatgcac cgaaaaagcg
     9781 tctgtttatg tgaactctgg caggagggcg gagcctatgg aaaaacgcca ccggcgcggc
     9841 cctgccgttt tgcctcacat gttagtcccc tgcttatcca cggaatctgt gggtaacttt
     9901 gtatgtgtcc gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc gagtgagcga
     9961 ggaggcgcaa tttccccggc acctgcaaga cggtcaccgg acgcgctgat gtgggatatt
    10021 gtcccctgat taagtctttc tattccctga cgatcaatta
//
