LOCUS       NZ_CP098426             5598 bp    DNA     circular CON 22-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 220 plasmid p220_3, complete sequence.
ACCESSION   NZ_CP098426
VERSION     NZ_CP098426.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28747376
            Assembly: GCF_032878355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Bellinzona,G., Sassera,D., Corbella,M. and Merla,C.
  TITLE     Klebsiella pneumoniae AVI/CZA and CFDC resistant isolated in IRCCS
            Policlinico San Matteo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Bellinzona,G., Sassera,D., Corbella,M. and Merla,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) Biologia e Biotecnologie L. Spallanzani,
            University of Pavia, Via Ferrata, 9, Pavia, Italy 27100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.78X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/22/2023 13:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,329
            CDSs (total)                      :: 5,208
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 54 of 118
            Pseudo Genes (incomplete)         :: 55 of 118
            Pseudo Genes (internal stop)      :: 26 of 118
            Pseudo Genes (multiple problems)  :: 13 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098426.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="220"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p220_3"
                     /geo_loc_name="Italy: Pavia"
                     /lat_lon="45.19660 N 9.14881 E"
                     /collection_date="2021-03-19"
                     /collected_by="Fondazione IRCCS Policlinico San Matteo"
                     /genotype="ST101"
     gene            join(5557..5598,1..135)
                     /locus_tag="NCW69_RS26590"
                     /old_locus_tag="NCW69_26525"
     CDS             join(5557..5598,1..135)
                     /locus_tag="NCW69_RS26590"
                     /old_locus_tag="NCW69_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165764108.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSV
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            125..469
                     /locus_tag="NCW69_RS26595"
                     /old_locus_tag="NCW69_26530"
     CDS             125..469
                     /locus_tag="NCW69_RS26595"
                     /old_locus_tag="NCW69_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            678..>1112
                     /locus_tag="NCW69_RS26600"
                     /old_locus_tag="NCW69_26535"
                     /pseudo=""
     CDS             678..>1112
                     /locus_tag="NCW69_RS26600"
                     /old_locus_tag="NCW69_26535"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1147..1656
                     /locus_tag="NCW69_RS26605"
                     /old_locus_tag="NCW69_26540"
     CDS             1147..1656
                     /locus_tag="NCW69_RS26605"
                     /old_locus_tag="NCW69_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1663..1875
                     /locus_tag="NCW69_RS26610"
                     /old_locus_tag="NCW69_26545"
     CDS             1663..1875
                     /locus_tag="NCW69_RS26610"
                     /old_locus_tag="NCW69_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2251..2379)
                     /locus_tag="NCW69_RS26615"
     CDS             complement(2251..2379)
                     /locus_tag="NCW69_RS26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            2542..2766
                     /locus_tag="NCW69_RS26620"
                     /old_locus_tag="NCW69_26550"
     CDS             2542..2766
                     /locus_tag="NCW69_RS26620"
                     /old_locus_tag="NCW69_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2750..3016
                     /locus_tag="NCW69_RS26625"
                     /old_locus_tag="NCW69_26555"
     CDS             2750..3016
                     /locus_tag="NCW69_RS26625"
                     /old_locus_tag="NCW69_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3086..3436)
                     /locus_tag="NCW69_RS26630"
                     /old_locus_tag="NCW69_26560"
     CDS             complement(3086..3436)
                     /locus_tag="NCW69_RS26630"
                     /old_locus_tag="NCW69_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3502..4047)
                     /locus_tag="NCW69_RS26635"
                     /old_locus_tag="NCW69_26565"
     CDS             complement(3502..4047)
                     /locus_tag="NCW69_RS26635"
                     /old_locus_tag="NCW69_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433973.1"
                     /translation="MFFKRYLTEKEIYKVALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4073..4399)
                     /locus_tag="NCW69_RS26640"
                     /old_locus_tag="NCW69_26570"
     CDS             complement(4073..4399)
                     /locus_tag="NCW69_RS26640"
                     /old_locus_tag="NCW69_26570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197760.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINEMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(4759..4917)
                     /locus_tag="NCW69_RS26645"
     CDS             complement(4759..4917)
                     /locus_tag="NCW69_RS26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          5391..5471
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="98.0"
                     /evalue="3.1e-22"
     Typing          complement(4809..5206)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          460..1991
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcctggcatc cggcagtgat ggcaaatctg atgcgtcagg cacaggagga agattaccgc
       61 cgggagcaga tggcagagct tgagcgttca gtccggtcac attttgcgga tatcggagga
      121 gaggatggcg gttaagcgta cccgtttttt aggtatccgg gtgaccgatg gggagtacca
      181 gcagctgctg gagcgctgca atggaaggca actggcggtg tggatgcggg aaacctgtct
      241 cgatacgcgc ccggcgcggt cgttgcggct gccgtccatc gatccggttt tgctgcgcca
      301 gcttgccggg atggggaaca acctcaacca gatcgcccgg aaaattaacg gcggccagtg
      361 gtcaggcgct gaccgggttc aggtggtggc cgcgctgatg gccatcgatg ccggactcga
      421 gcggctgcgg catgccgtgc tggaaaaggg ggcagacgat gatcgttaag tttcatccgc
      481 gagggcgagg cggcggtgcc gggccggtgg attatctgct ggggaaagac cgccagcgcg
      541 acggggccat cgtcctgcag ggaaagccgg aggaggtccg ggagcttatc gacgcctcgc
      601 cctacgccaa aaagtacacc tccggcgtcc tgtcttttgc tgaacaggat ttaccgcccg
      661 gccagcgtga aaagctgatg gcgagcttcg agcgggttct gatgcccgga ctcgataaag
      721 accagtacag cgtgctgtgg gttgaacacc gggacaaggg gcggctggaa ctgaattttc
      781 tggtaccgaa cacggaactg ctgaccggca ggcgtctcca gccgtactac gaccgggcag
      841 accgtccgcg catcgatgcg tggcagaccg tggtgaacgg gcgactgggg ctgcacgacc
      901 cgaacgcacc ggagaaccgg cgcgcgctgg tgacgccatc ttcgttaccc aaaacgaagc
      961 aggaagccgc agaggctatt acgcggggct tactggccct cgcctcgtca ggggagctta
     1021 aaacgcgtca ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtc gtgcgcacca
     1081 caaagagcag catcagcatt gccgacccgg acggagggcg aaacatccga cttaagggag
     1141 ccatctatga acagtctttt aacgctggcg aaggacttag agcagaaatc gaaagcgcag
     1201 cagcagagta ccggcgaaat gctgaaagcc gcattcagcg agcacgagaa gtctgtcaga
     1261 gcggaactga gcgaaagcgg gaagagaatc agcgccgcca tccgcgccca cgagcagggt
     1321 atgagcgcgg ccatgcagtc gaaccgcctg agcgtgatgc gtatggtcgg gcggacatgg
     1381 ctgaccatca cgatggtgtc aggactgctg ttcgccagcc tgagcggggt gttgtggtat
     1441 caggggagcc tgatagcgtc aaatctggcc gaaatcgacc ggcagaacgc ggcgctgtcg
     1501 aagctgaacg cgaagacctg gggcgtgacg tacctggaag acagcaacgg acgttttctg
     1561 gtgctgccga aggggacggc cgtagacagg acgcagagct ggacggtcgg gaacgggcgg
     1621 tcgaagcaga acgcgctgag actggtgaag gagtaagtgc cgatgaccga gctggaaaaa
     1681 cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct ggacgagtgg
     1741 gagagcgcct tcggggaatg gcggaaaatg tcagggctta tgcaacggga gaacgcggcg
     1801 ctgagcgagc gcgtgacgcg cttgagtcag caggtggaac gcttgagcgg gcaactgcag
     1861 cgtttgagcc ggtagtgcag cgtcatgaga tggccgtggc cgcagaacgg gcgcacgagc
     1921 aacggcagca cgaaaaggaa ctgacggaag cgcgctcccg gcagcgaagc tatgacggtc
     1981 cgtcgctggg ctgaaatgca ggaaaacagg gttctcatcg ggcatttacg ctgaaaccgg
     2041 atgtcattca cctggctgca tggctatgca gccaggtaaa atttatctgt cgcgaacggg
     2101 gcggtttatc gggtggtttg ttgccggttt tacctgactg ccaccccgtt cgcggcgaac
     2161 ccgtccgggg cggtgcgggc aacgggtgtt atgtttaatc agcgtgaacg gcagatacaa
     2221 aaaagccccg gagggctttt aactactgac ttattttttt gaagatttgc tgggtttcaa
     2281 tttgtcagta tttcccggcg tttttttatt gtcacgctgg cgtttatcag gttcccctgt
     2341 tgaatcggca attcgtttcg gacccggctt aagacccata atcgttatcc tttcaatagt
     2401 taagaggaaa gccctcaagc tagatatatt gaccgcgtta cggattatca agaggggcta
     2461 acaagattat tttttgttct tttaggttga ttgtggtact gttgatatac atgtatagca
     2521 aatggtgttc gggaggttta tatgcttgct atccggttat ctgatgagat tgagtcccgt
     2581 ctggactcgc tggcgaagca aaccggcaga acaaagacgt tttatgcgcg ggaagcaata
     2641 ctggcgcatc tggaagacct ggaggattat tatctttcag cagaaactgc tgcacgcgtt
     2701 cgccgtggtg atgaagcagt gcattcgtct gaagacgtga ggaagtcact tggtctggac
     2761 gattaactat tccgatcggg cgctcaaatc gttacgcaag atggacaaac agaacgcacg
     2821 acggattgtg gattttatga gtttacgcat tgcagttgct gccgatcctc gccagtcagg
     2881 gaagccgctc aaaggtgagc tgggcgagtt ctggcgctat cgggtgggag attatcgcgt
     2941 tctgtgtgag atccgagatg acgagcttgt tatccttgcc gccacgattg gacatcgccg
     3001 cgaagtttac gactgaagcc cgctatgcgg gcttttttga tgccgacaac aggtattatg
     3061 gtaaatactc gactaactac gtgttctact gtttgtcggt tttagttaac cagcaggttg
     3121 tcttaacgat aatgtagccg ttatttttaa ctaataaggt catattgtta acgtcaggca
     3181 taataaaaga cattatggcc tcagagttat catcagaagc agctctctgc attactactt
     3241 cgattccctt ttctttgaat ctataagtat ccagttgcct attaaactta gaaagaccta
     3301 caaccatact tagtttgtta tctttaacag taagagatgc ttcctgagta ccattacatc
     3361 ggtagtaagc ggtgtaaccg tcagcaaaag atcgaccggg gaatataaat gctaggaacg
     3421 caattaacca atacatagtt gagaatcttg gtattacaca tttcactatc ctctctttca
     3481 tttttcgttc accatacttt gttatctgat ttttaatttt tctattttat aaaccaagtc
     3541 cctaccaaaa aggcgaaaat tatcctcaaa taataataat tgtttaagaa catccatgaa
     3601 agcagctgct tcaccctcag ttatgggagc tactctatat aacatataac ctgagttaag
     3661 ggtttctttt atactttctt caatgctcat tataaatatt agtggattat catttttctt
     3721 cccccatggg tttctaatta atccttcgat gtactcaggt cctttgcgtc ctattgcctg
     3781 cgcaatgttt tttatttcat ctgcgtgagg gctttgttgt acagctttgt ttatgcctgc
     3841 ttgagtgatt ttcagagact ctacaccgtg tatgttgtat gagataaaat gtaataaatt
     3901 aagatgtaat gcaattaatc catttttgtt tacatctgag tatttggata aaaaacgtaa
     3961 tgaagtgtta actctccctt gaagcctaac aattgggagt gctaccttgt atatttcctt
     4021 ctccgttaaa taccgtttaa aaaacataat tctccgcctc cgtatagcgt gcctatttta
     4081 tttcgtcgta aaacttagct ctgaaatatt cctttagaac catcatttca ttaataattg
     4141 aatctatctc atccattttt tcttggtatt cttttaatgg tgaagggagc ggtaactcaa
     4201 tatttgaaaa gtgattggcg agatagggat caaggtttct catccattca ggcttttcgc
     4261 caatgcgttc ttcaactgaa agtgaacctg ataatctctg gttaataaga tcacttagac
     4321 tcattttggt tttattcgaa agccgttgta aggattgctt taatcctata tctagcatga
     4381 tatttgttct ttctctcatt accccccccc tcgctattgc gttttttgtt gcgcacaaag
     4441 ctaccattgc gcattaagaa acgcaataaa aaaggaggga gggagtggtt tgtacatgat
     4501 gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg tgggatggat ggagctccgg
     4561 ggataacaga actgacgatc atttaaagga tcttcttgag atcctttttt tctgcgcgta
     4621 atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc
     4681 agttggggaa ctgcttaacc gggtaactgg gtatacgaga ccgcagatac caaaaatgtt
     4741 ctttcagtgt agccgcagtt aggcacccac ttcaagacct ctcaatatct ctctcgtata
     4801 tatccagtta ccaatggctg ctgccagtgg cgtttttgcg tgtccgtccg ggttggactc
     4861 aagatgatag ttaccggaaa gggcgcagca gtcgggctga acggggggtt cgtgcataca
     4921 gcccagcttg gagcgaacga cctaaaccga gccgagatac cagcagcgtg agctatgaga
     4981 aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt attcggtaag cggcagggtc
     5041 ggaacaggag agcgcacgag ggagccgccg gagagaaatc accggcatct ttaagtcctg
     5101 tcgggtttcg tcctcctctg acttgagcgt ccatttctgt gatgctcgtc aggggaggcg
     5161 gagcctatgg aaaaacggcc acgccgtatc cttttctccg gcactttctg atttgagcgc
     5221 ctttgcttta tcccgttatc cgtgagccgg tcacgcccgc cgcagccgaa cggcaggagc
     5281 gcagcgaccg agtgagcgag gaggcgtcat ttcatctgtc cgtgcatggt attgcggacg
     5341 ctcctgagtc acatatcaca cctgccacat gaggcacttt catgaatctc gctctcatgc
     5401 caacatagta agccagtata cacagcgtaa acgctgtgac tggttcaggg ctgcgccccg
     5461 aaacccgcta acaccactga cgcgctggcg cttgtccaac gccggcgcgt cccggaaaac
     5521 atttccgtgc cgtccggcgt ttgcgaagga gggataatgc agaagccgaa gaagaagccg
     5581 ctcgtcgtaa aaaaaaga
//
