LOCUS       NZ_CP098425            10122 bp    DNA     circular CON 22-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 220 plasmid p220_2, complete sequence.
ACCESSION   NZ_CP098425
VERSION     NZ_CP098425.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28747376
            Assembly: GCF_032878355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Bellinzona,G., Sassera,D., Corbella,M. and Merla,C.
  TITLE     Klebsiella pneumoniae AVI/CZA and CFDC resistant isolated in IRCCS
            Policlinico San Matteo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Bellinzona,G., Sassera,D., Corbella,M. and Merla,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) Biologia e Biotecnologie L. Spallanzani,
            University of Pavia, Via Ferrata, 9, Pavia, Italy 27100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.78X
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/22/2023 13:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,329
            CDSs (total)                      :: 5,208
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 54 of 118
            Pseudo Genes (incomplete)         :: 55 of 118
            Pseudo Genes (internal stop)      :: 26 of 118
            Pseudo Genes (multiple problems)  :: 13 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098425.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="220"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p220_2"
                     /geo_loc_name="Italy: Pavia"
                     /lat_lon="45.19660 N 9.14881 E"
                     /collection_date="2021-03-19"
                     /collected_by="Fondazione IRCCS Policlinico San Matteo"
                     /genotype="ST101"
     gene            join(10095..10122,1..83)
                     /locus_tag="NCW69_RS26525"
                     /old_locus_tag="NCW69_26460"
     CDS             join(10095..10122,1..83)
                     /locus_tag="NCW69_RS26525"
                     /old_locus_tag="NCW69_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_116938357.1"
                     /translation="MTTDYLQQCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            1564..3528
                     /locus_tag="NCW69_RS26530"
                     /old_locus_tag="NCW69_26465"
     CDS             1564..3528
                     /locus_tag="NCW69_RS26530"
                     /old_locus_tag="NCW69_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3528..4259
                     /locus_tag="NCW69_RS26535"
                     /old_locus_tag="NCW69_26470"
     CDS             3528..4259
                     /locus_tag="NCW69_RS26535"
                     /old_locus_tag="NCW69_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            4266..4796
                     /locus_tag="NCW69_RS26540"
                     /old_locus_tag="NCW69_26475"
     CDS             4266..4796
                     /locus_tag="NCW69_RS26540"
                     /old_locus_tag="NCW69_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4824..5003)
                     /locus_tag="NCW69_RS26545"
                     /old_locus_tag="NCW69_26480"
     CDS             complement(4824..5003)
                     /locus_tag="NCW69_RS26545"
                     /old_locus_tag="NCW69_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5072..5449
                     /locus_tag="NCW69_RS26550"
                     /old_locus_tag="NCW69_26485"
     CDS             5072..5449
                     /locus_tag="NCW69_RS26550"
                     /old_locus_tag="NCW69_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(5511..5810)
                     /locus_tag="NCW69_RS26555"
                     /old_locus_tag="NCW69_26490"
     CDS             complement(5511..5810)
                     /locus_tag="NCW69_RS26555"
                     /old_locus_tag="NCW69_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5800..6063)
                     /locus_tag="NCW69_RS26560"
                     /old_locus_tag="NCW69_26495"
     CDS             complement(5800..6063)
                     /locus_tag="NCW69_RS26560"
                     /old_locus_tag="NCW69_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6369..6788)
                     /locus_tag="NCW69_RS26565"
                     /old_locus_tag="NCW69_26500"
     CDS             complement(6369..6788)
                     /locus_tag="NCW69_RS26565"
                     /old_locus_tag="NCW69_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7191..8876
                     /locus_tag="NCW69_RS26570"
                     /old_locus_tag="NCW69_26505"
     CDS             7191..8876
                     /locus_tag="NCW69_RS26570"
                     /old_locus_tag="NCW69_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8886..9143
                     /locus_tag="NCW69_RS26575"
                     /old_locus_tag="NCW69_26510"
     CDS             8886..9143
                     /locus_tag="NCW69_RS26575"
                     /old_locus_tag="NCW69_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            9228..9464
                     /locus_tag="NCW69_RS26580"
                     /old_locus_tag="NCW69_26515"
     CDS             9228..9464
                     /locus_tag="NCW69_RS26580"
                     /old_locus_tag="NCW69_26515"
                     /inference="COORDINATES: protein motif:HMM:NF014457.3"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_123836186.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANGTVAKLAMRQPFVLFKGLTF
                     QKLCLPGAFRPGDHHNKMLRPGLCVVHASPQYL"
                     /name="colicin release lysis protein"
     gene            complement(9355..10059)
                     /locus_tag="NCW69_RS26585"
                     /old_locus_tag="NCW69_26520"
     CDS             complement(9355..10059)
                     /locus_tag="NCW69_RS26585"
                     /old_locus_tag="NCW69_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     BGC             2191..10122
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(187..765)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3529..4256
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 aggcaaacta tatccgggat gttcagggtg gaacggtggc accatcgtcc tcctctgaac
       61 tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct
      121 gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg
      181 ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca
      241 aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta
      301 gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg
      361 tgcttttccg ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga
      421 acggggggtt cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac
      481 cagagtgtga gctatgagaa agcgccacac ttcccgcaag ggagaaaggc ggaacaggta
      541 tccgggaaac ggcagggttg gaacaggaga gcgcaagagg gagcgattca tcggaaacgg
      601 tgatgatctt taagtcctgt cgggtttcgc cactcctgac tgattcatgg ttgagccacc
      661 ggctcccaca gatgcaccga aaaagcgtct gtttatgtga actcagtcag gagggcggag
      721 cctatggaaa aacgccacct gcgtggcctt gccgttttgc ctcacatgtt agtcccctgc
      781 ttattcacgg aatctgtggg taactttgca tgtgtccgca gcgcccgccg cagtctcacg
      841 gccggagcgt agcgaccgag tgagcgagga ggcgcaattt ccccggcacc tacaagacgg
      901 acaccggacg cgctgatgtg gcatattgtc ccctgattaa gtctttatat tccctgataa
      961 taaattacca ggataggtga atcatatcgg tttttcgctg aacattttac gttaacctat
     1021 ctctgttgct tttttctcct tacggagaaa tacaggtcat ccccgcgagg gcgtggctga
     1081 cctcaggggg atatcccctg cttacgctgg ctggacgcca ccgacgcacg gtctctcccc
     1141 ctctttctgg ctccgtccgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc
     1201 aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg acgcacctgg cgaagcagca
     1261 aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc
     1321 ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc
     1381 tcgttgttcc tccggggctg cgccttttcc gcatggggcg gaaagaggct ggcctccgtg
     1441 gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc
     1501 cgcggcttgt gcagcggggg ctgcacatca cgccggcaaa ccatcgtctg aaggagagag
     1561 aacatgttta atacggattc gcttgcctgg aagtggcaca ttccggagct ggcccggcag
     1621 atttatccgt gggtcagctg gctgtaccag gagtattacg gtctggccgg ggctttccta
     1681 gttatcggcc tgtttttcgg tctggctgaa ctttgggccg gacggatgcg ttttgtgggg
     1741 ctgagtcata aggagcgacg ggagtatgcc gatcgctata agcgcgataa taaacggcca
     1801 gcgccatttt cggtgctggc ctcgctggat tcgcccgggc ggatggtcag ggcacgtcga
     1861 catgtctgga actacgcgta ctggctgacc ggcgcggccg gtctggccct tctggggggg
     1921 ctggttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgtcc ggacctctac
     1981 gcaaacgcag attattacct gctgcagccg gctaacctgt tcgggattgc ctggtgcgcg
     2041 ggcgggctgg ctgtcgggat tgtgttggcg cggctgctgg cgttcctgct gcttgacggt
     2101 cattttgcgg ctgctgacga ggcggtgaac gcgaaactga atcaggagag ccggcgcagc
     2161 agccagcgga caggagagat gaccgacgtg cgccacctgc atttcgggga gccggtaccg
     2221 gttaatgcgc tggccgattt ttcctcggag caggcccggc aggagcaggc ggtgtttctg
     2281 ggtaaggatg agcagggcaa gccggtgctg gttccgcgtg atacctggcg caagaccaac
     2341 attcagatcc tcggcctgcc cgggagcggc aagagcgtga tggccaccaa cgcgctcatt
     2401 cgctgcgtac gcgatttcgg ggacgcggtg gtgtacttcg accccaaagg ggacgcatgg
     2461 gcgccgcacg tcttccgggc gcactgtccg gacttcaccc tgctggatct gcggccgggg
     2521 aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgcgctgaa aaacctgctg
     2581 attgccgggc ttaacctggg cgagaccggc gacgtggcgg accactaccg catcagcgag
     2641 cagaaagcgg cgaagctgat tgcggagcag tttccgcagg gggcaaacat tcagcaggtg
     2701 ctggcggcgg cgtacgcgct gccggaggcc ctgaaaaagg acgtgaaggg gttgatcact
     2761 aggctggaaa acgtcgctga cctgagcgtc ctgcagacgg acagtggcat cgatgtggcg
     2821 gggattatca acggcggcgg ctgcctgtac gttatcggct cgatggacga cgaggcggtg
     2881 atccgtgtgc aaaaaatgct gtttgcccgc tgcgcgcaaa tcatcattgc gcgggacgag
     2941 ttccgggagt ggccacacgt cagcgtgatg ctcgatgaaa ttaaatatct gctgtcgaag
     3001 tatgtactga acgcactggg gacgctgcgc agccgggact gcaatctgtt gctcgcgcac
     3061 cagtcgctgg gcgatttcgg gcagtgcggg caggatctgc ccgctgaatt tgtgaaaacc
     3121 acggtactgg ataacacgcc gatccgctgg ttttaccggg cggccagcct ggagtccgcg
     3181 cagtgggcgg cggggcagac cggggaaatc agggtggacg tggagcgccg ccgtgccagc
     3241 cgggaggcgg ggaacgtgga gcatatcagc ggggacagct tcatccagaa ggaggcgcgg
     3301 ccgctgtttg acgtgaacac cctgcaacac ctgccggacg ggttcgcggt catgaccggg
     3361 ctgggtgtgg cccggatggc gttcagcagt ccgctgcggg ttgagcgccg ggaaatcccg
     3421 ttgcagcgtt tcccggtgct ggcgaaaacc gatcccctgg cggagttccg gcccccggcc
     3481 gacggcggcg ggccgaaagc ggatgatgat tttgcgggga tttactgatg gcgctggagc
     3541 tgtacaacgt cagccacgcg aaacgacagg cgcggaacgc ggagaaaacc cggctgaccc
     3601 tgcgctggtt gcgggaagag ctgtgttcca cggcggagct ggttgcgcgg cggctgggga
     3661 ttgcggcggt gcagccggtc taccgcttcc tcgacagcct ggtggcgaaa gggctgctgg
     3721 tccggaccaa atatccggtg gacggccgtc aggtcagcgt gtgggggctg acgccgcatg
     3781 gcgtggcgtt ctcgttcgac gaggacgaac cgctggcgga tgttattccg tttcagccgt
     3841 caagggtgtc ggcagcacat ctgccgcacc ggctggccgt gcaatccctg cgccttgata
     3901 tggaagccag aggggccacc ggctggcgca ttctgcaccg tctgagtctt aaaggcatga
     3961 aagtgccgga cgccctggcg gagctggacg gtaaaacggt ggcgtttgag gtggagcgca
     4021 ccgtcaagag ccgtcgccgg tatcaggaag tggtgtccgg gtatctgttc aaccgcaaag
     4081 ctaacggcat cgatgaaatc tggtacatct gcccggaccg ggcgacgcag gtcagggtgc
     4141 agcgggcaat cctgtcggtg gatgaaatcg tgaacccaca gaccggcgag gcccgcaaaa
     4201 cggcagagct ggatcgtgaa cggttgtttg cgtgctttaa atttatgaca acggagtgag
     4261 ggattatggc gctgtacatg gtatttattg aagacgcgga ggaccggaac ggcgagcgct
     4321 gcatcatgcc gaccagcgag catctggaag atatggtggt gctcgcctgg cagaccgggg
     4381 cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa tccatcggga
     4441 tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcact ggtaaaaatc
     4501 cgcaatggga acggaacaaa cccacaggaa tgaagctgtg tgattacctc tatcgcaagg
     4561 tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg
     4621 agcagattaa cgcgatgatt gacgtggtga gtgagcggca cggggaaagc gcggtgaacg
     4681 agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtac
     4741 ctgaactgcc ggaggacaaa cctgcccgcc ggtggtggcc gttcggcaaa aaataaatgc
     4801 cagcaccggc catgccggtg ctgttattcc tccccgagct tatcccgcat cacctgccag
     4861 agtgtttccg catcctcgtg tagccgctcg cacatgtcgg ccggggtatc catatccagc
     4921 tcattcagtt tatcgagcaa tagcagcgac tgctccttaa taaatttagc catattgagg
     4981 gctgtttttt cctgttttgt catgattctt tccttccggg catggtgtgc gcctgtcagg
     5041 tatactgtct caaaaatagc ggggtgagac aatggcgtgg gtgacggtga gacaggcgac
     5101 ggaactgacg ggtaaagccc gaagttccct gtatcgggat atggcgaaag gccgcgtatc
     5161 ataccgtacg gaagctgatg gcggccgggt ggtggatacc agcgagctta tccgggtgta
     5221 cggtgagtta agacaggatg agactcacga gcgggacgag ttgagactgt cgggtgagac
     5281 tgaaaaaatg gcctccgacg cgttgatcgc ggagataaaa gcactcaggg aggaggtggc
     5341 cgggctacgg caggaaatgc agtccatgag actgctggaa cacaaaccgg agacagcaga
     5401 gaccccggcc ggaacaccgc gccggtggtg gccgtgggga aaacgctgac gcgctgtgct
     5461 tgtccaaccg gcgacggcca cgcaatgaat tgcgtccccg tcaccggtta ttagagaaca
     5521 atatggggat tctggcgctg gccgaccacg gccagaaccc ggacttttct ggtctggtca
     5581 tcaacatcat agaggacatg ctgtcccatc agactgatcc ggcgaacgtc ctcgccatag
     5641 cgtgagtcca cttcatagag tcgcgggaag tcggccagtt tgtccgccgc agcgagaacc
     5701 cggtcggtaa cgttctgcgc atcccacagg gatgaatttt ccgcgatata gtcaaacagc
     5761 gtcgttacat cctcgatggc tccctgcttc cattccacgt catacggcat cgcgcagttc
     5821 ctgataacgg ccatgcaggc gctggcggag ttgttccgtg gtcatacccg gacgcgtgtc
     5881 agcacgggcg gcggtcactt tggcgcgaac ctgatcttca tactctgcct gggccagttc
     5941 ttcgttagcc agtcgctgca acagcgcagc tttgtccggg ccaccttccg gcagtatgcg
     6001 gggcaggctt tcagccagtt cccgaagaaa gttggcgttt tcgtagtgtg tagtgttgct
     6061 cattgcggag cctcccgggt taatgcggca atatgaagga agtttagcag ggggcggtct
     6121 gaaagaatat ggtcgtgtca gtcaggctga tgaagcagaa agtcggtaaa aaagggctta
     6181 cggcatccat tttacgtcaa aacatatgcc atgcagttgc gtgcataggc atgcattagg
     6241 ataaaattta tcgggcgcgt ttccggcagt tttccggggg ggttgttgcc tgttttatcc
     6301 cgtagccgcc ggaaacgccc tgagcccgtc tgagcggtgc gcgtaatgac gcgttatggt
     6361 aaatccactt actgtctgcc ttcatagcca tcgagataaa cagctgaaat ggtgtcagcc
     6421 agtagccttg attttttcgg tgtgaggtct ttgctgcggt aatcggtacc cgcaaattgt
     6481 gttgtgcctt ggagtgagtt taaaaattcc tgactgtgaa actgctgaat gcgttgctgc
     6541 acatcactcc gtgattttcc ggtcgcttta tcctttgcgc ccatatccca gaactgccgg
     6601 aaaaaaggca ccatcattcg tgttgtgtca gctttctggg tggaaaaatt gggatcaaaa
     6661 ccgtcatctg aagcatatac gtaatgccgg gtatgctgtt ccacgctgcc ctttggagca
     6721 caggcagtga gagttgctgt cgctgttatc atggcgataa gacacagttt gctgccggaa
     6781 cgtctcatga gcgtctcctt acctttaatt cttttttctc attgagttat atatccgcag
     6841 gctatcttat catcagtttc tgaccgtgtt cagtgaccga acaacccgaa ccagaccccc
     6901 cgtttctttt cctgtcccgg agggggcttt ctggtatctc atataatgat ttcagtaaaa
     6961 catactgagg ccggagaacg gcgccgtttg tgctggtttt tgttgcatct ggaggggatt
     7021 gcgcatggtg tgagtggctg tttttttgat cattttcaca aaaacgcaaa tttacagact
     7081 tgacatgaaa aacaaggcag cgtaattttt gtactgtata taaaaccagt ggttatatgt
     7141 acagtatttt attttagttt attgttttaa aagtcaaaga ggaaaagtat atgagtggcg
     7201 gagacggacg aggtccgggt aattcaggtc tgggacacaa tggtggtcag gccagtggga
     7261 atgtgaacgg tacatcaggt aaaggtggcc cttcatcagg tggtggtacg gatccaaaca
     7321 gcgggccggg ctggggtacg acgcatacac ctaacgggga tattcataac tataacccgg
     7381 gggagtttgg taacggcggg agtaaacccg gtggtaatgg cggtaacagc ggcaatcata
     7441 gcgggagctc tggtggcgga cagtcttcgg ccaccgcgat ggccttcggt ctgcctgccc
     7501 tggccactcc gggggctgaa ggactggctt tatccgtttc cggcgatgcg ttgtcggccg
     7561 ctgttgctga tgtgctggct gccctgaaag gtccgtttaa gtttggtctg tgggggattg
     7621 cgatttacgg agtgctgcct tctgagatag caaaagatga tccgaaaatg atgtcaaaaa
     7681 tcgtgacgtc attgccggca gacacggtaa cggagacgcc ggtcagctcc ctgccgctgg
     7741 accaggcgac ggtcagcgtc actaaacgtg tggcggatat tgtgaaggac gagcggcagc
     7801 atattgcggt tgtcaccggc cggccaatga gtgttcctgt ggtggatgcg aaaccgacaa
     7861 aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac
     7921 ctaaaggtgt tccggccgcg aaagccccgc caaaaggcat tattgctgaa aaaggtgatt
     7981 cacgtccggc tggttttacg gccggtggta actcccgtga ggccgttatt cgtttcccga
     8041 aagagagcgg acagaaaccg gtctatgtgt cggtgacgga tgttcttact ccggcacagg
     8101 taaaacagcg tctggaggaa gaaaagcgtc gccagcaggc atgggacgct gctcacccgg
     8161 aagaggggct gaaaagagag tatgataaag cgaaagctga gctggatgct gaagataaaa
     8221 atattgcgac cttaaacagt cgcattgcat cgacagagaa ggcgatcccc ggtgcaaggg
     8281 ctgctgttca ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg
     8341 tgacttataa cccgcctcat gaatatggct ccgggtggca ggatcaggtt cgctatcttg
     8401 ataaggatat tcagaatcag aatgagaaat taaaagctgc ccagacatct ttaaacgaaa
     8461 tgaatgaatc cttatccagg gataaggctg cgctttccgg ggcgatggag agccggaaac
     8521 aaaaggagaa aaaagcgaag gatgcagaaa ataaattaaa tgaggaaaag aaaaaacctc
     8581 gtaagggaac taaagattac ggccatgatt attttcctga tcccaagact gaagatatta
     8641 aggggttggg agagttgaaa gagggtaaac ctaaaacccc taaacaaggt ggtggtggta
     8701 agcgggcccg atggtatggt gataaaaagc gtaaaattta tgaatgggat tcccagcacg
     8761 gtgagcttga agggtaccgc gccagtgatg gcgaacacct cggggcattc gatccaaaaa
     8821 cgggtaagca ggttaaaggg ccggatccaa aacgaaacat taaaaaatat ctttaagagg
     8881 taaatatggg gcttaaatta catattcatt ggtttgataa gaaaaccgaa gagtttaaag
     8941 gtggtgaata ctcaaaagac ttcggtgatg atggttctgt cattgaaagt ctggggatgc
     9001 ctttaaagga taatattaat aatggttggt ttgatgttga aaaatcatgg gtttcgatat
     9061 tacagccaca ctttaaaaat gtaatcgata ttagtaaatt tgattacttt gtatcctttg
     9121 tttaccggga tggtaactgg taagcaaaag ttatcaagga tgatttgaaa tacagacttc
     9181 cggtgttcag ggatgaatac cggggttttt aaatacaggg aaggtatatg aaaaaggtaa
     9241 aggttgtttt tctttttata ttgatttttt caggctttct tctggtcgca tgtcaggcaa
     9301 acggcactgt tgcaaagtta gcgatgaggc agccttttgt cttattcaaa ggccttacat
     9361 ttcaaaaact ctgcttacca ggcgcatttc gcccagggga tcaccataat aaaatgctga
     9421 ggcctggcct ttgcgtagtg cacgcatcac ctcaatacct ttgatggtgg cgtaagccgt
     9481 cttcatggat ttaaatccca gcgtggcgcc gattatccgt ttcagtttgc catgatcgca
     9541 ttcaatcacg ttgttccggt acttaatctg tcggtgttca acgtcagacg ggcaccggcc
     9601 ttcgcgtttg agcagagcaa gcgcgcgacc ataggcgggc gctttatccg tgttgatgaa
     9661 tcgcgggatc tgccacttct tcacgttgtt gaggatttta cccagaaacc ggtatgcagc
     9721 tttgctgtta cgacgggagg agagataaaa atcgacagtg cggccccggc tgtcgacggc
     9781 ccggtacaga tacgcccagc ggccattgac cttcacgtag gtttcatcca tgtgccacgg
     9841 gcaaagatcg gaagggttac gccagtacca gcgcagccgt ttttccattt caggcgcata
     9901 acgctgaacc cagcggtaaa tcgtggagtg atcgacattc actccgcgtt cagccagcat
     9961 ctcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
    10021 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
    10081 gggatgataa gtttatcacc accgactatt tgcaacagtg cc
//
