LOCUS       NZ_CP098344             9294 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 53009_G1 plasmid p53009-9.3, complete
            sequence.
ACCESSION   NZ_CP098344
VERSION     NZ_CP098344.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28680537
            Assembly: GCF_023658165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Chen,L.
  TITLE     Genomic Characterization of Multidrug-Resistant Enterobacterales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2022) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023658165.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:22:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,713
            Genes (coding)                    :: 5,571
            CDSs (with protein)               :: 5,571
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 53 of 142
            Pseudo Genes (incomplete)         :: 93 of 142
            Pseudo Genes (internal stop)      :: 14 of 142
            Pseudo Genes (multiple problems)  :: 17 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098344.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="53009_G1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p53009-9.3"
                     /geo_loc_name="USA"
                     /collection_date="2017/2018"
     gene            450..2414
                     /locus_tag="NB668_RS27295"
                     /old_locus_tag="NB668_27290"
     CDS             450..2414
                     /locus_tag="NB668_RS27295"
                     /old_locus_tag="NB668_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            2414..3145
                     /locus_tag="NB668_RS27300"
                     /old_locus_tag="NB668_27295"
     CDS             2414..3145
                     /locus_tag="NB668_RS27300"
                     /old_locus_tag="NB668_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            3152..3682
                     /locus_tag="NB668_RS27305"
                     /old_locus_tag="NB668_27300"
     CDS             3152..3682
                     /locus_tag="NB668_RS27305"
                     /old_locus_tag="NB668_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3710..3889)
                     /locus_tag="NB668_RS27310"
                     /old_locus_tag="NB668_27305"
     CDS             complement(3710..3889)
                     /locus_tag="NB668_RS27310"
                     /old_locus_tag="NB668_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3958..4335
                     /locus_tag="NB668_RS27315"
                     /old_locus_tag="NB668_27310"
     CDS             3958..4335
                     /locus_tag="NB668_RS27315"
                     /old_locus_tag="NB668_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(4397..4696)
                     /locus_tag="NB668_RS27320"
                     /old_locus_tag="NB668_27315"
     CDS             complement(4397..4696)
                     /locus_tag="NB668_RS27320"
                     /old_locus_tag="NB668_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4686..4949)
                     /locus_tag="NB668_RS27325"
                     /old_locus_tag="NB668_27320"
     CDS             complement(4686..4949)
                     /locus_tag="NB668_RS27325"
                     /old_locus_tag="NB668_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(5255..5674)
                     /locus_tag="NB668_RS27330"
                     /old_locus_tag="NB668_27325"
     CDS             complement(5255..5674)
                     /locus_tag="NB668_RS27330"
                     /old_locus_tag="NB668_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6077..7762
                     /locus_tag="NB668_RS27335"
                     /old_locus_tag="NB668_27330"
     CDS             6077..7762
                     /locus_tag="NB668_RS27335"
                     /old_locus_tag="NB668_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7772..8029
                     /locus_tag="NB668_RS27340"
                     /old_locus_tag="NB668_27335"
     CDS             7772..8029
                     /locus_tag="NB668_RS27340"
                     /old_locus_tag="NB668_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            8114..8263
                     /locus_tag="NB668_RS27345"
                     /old_locus_tag="NB668_27340"
     CDS             8114..8263
                     /locus_tag="NB668_RS27345"
                     /old_locus_tag="NB668_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1077..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8367..8945)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2415..3142
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 acgccaccga cgcacggtct ctccccctct ttctggctcc gtccggctgg acgccggcca
       61 cgccagaaat tcacccggcc cccgtcaggc tcacgcctga ccacccacaa aaatccacgg
      121 tgctggacgc acctggcgaa gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct
      181 cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg gggtgccgcc
      241 ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc ttttccgcat
      301 ggggcggaaa gaggctggcc tccgtggtga aagctgaacg caatcaccac ggacgtgacc
      361 gccgccgcca tgccttgggg catgtccgcg gcttgtgcag cgggggctgc acatcacgcc
      421 ggcaaaccat cgtctgaagg agagagaaca tgtttaatac ggattcgctt gcctggaagt
      481 ggcacattcc ggagctggcc cggcagattt atccgtgggt cagctggctg taccaggagt
      541 attacggtct ggccggggct ttcctagtta tcggcctgtt tttcggtctg gctgaacttt
      601 gggccggacg gatgcgtttt gtggggctga gtcataagga gcgacgggag tatgccgatc
      661 gctataagcg cgataataaa cggccagcgc cattttcggt gctggcctcg ctggattcgc
      721 ccgggcggat ggtcagggca cgtcgacatg tctggaacta cgcgtactgg ctgaccggcg
      781 cggccggtct ggcccttctg ggggggctgg ttgtcgccgt gtttatcgag agctggggag
      841 aaaccgtcct gcgtccggac ctctacgcaa acgcagatta ttacctgctg cagccggcta
      901 acctgttcgg gattgcctgg tgcgcgggcg ggctggctgt cgggattgtg ttggcgcggc
      961 tgctggcgtt cctgctgctt gacggtcatt ttgcggctgc tgacgaggcg gtgaacgcga
     1021 aactgaatca ggagagccgg cgcagcagcc agcggacagg agagatgacc gacgtgcgcc
     1081 acctgcattt cggggagccg gtaccggtta atgcgctggc cgatttttcc tcggagcagg
     1141 cccggcagga gcaggcggtg tttctgggta aggatgagca gggcaagccg gtgctggttc
     1201 cgcgtgatac ctggcgcaag accaacattc agatcctcgg cctgcccggg agcggcaaga
     1261 gcgtgatggc caccaacgcg ctcattcgct gcgtacgcga tttcggggac gcggtggtgt
     1321 acttcgaccc caaaggggac gcatgggcgc cgcacgtctt ccgggcgcac tgtccggact
     1381 tcaccctgct ggatctgcgg ccggggaaac cggcgcagct gaatctgttc cgcgatctcg
     1441 accagtacgc gctgaaaaac ctgctgattg ccgggcttaa cctgggcgag accggcgacg
     1501 tggcggacca ctaccgcatc agcgagcaga aagcggcgaa gctgattgcg gagcagtttc
     1561 cgcagggggc aaacattcag caggtgctgg cggcggcgta cgcgctgccg gaggccctga
     1621 aaaaggacgt gaaggggttg atcactaggc tggaaaacgt cgctgacctg agcgtcctgc
     1681 agacggacag tggcatcgat gtggcgggga ttatcaacgg cggcggctgc ctgtacgtta
     1741 tcggctcgat ggacgacgag gcggtgatcc gtgtgcaaaa aatgctgttt gcccgctgcg
     1801 cgcaaatcat cattgcgcgg gacgagttcc gggagtggcc acacgtcagc gtgatgctcg
     1861 atgaaattaa atatctgctg tcgaagtatg tactgaacgc actggggacg ctgcgcagcc
     1921 gggactgcaa tctgttgctc gcgcaccagt cgctgggcga tttcgggcag tgcgggcagg
     1981 atctgcccgc tgaatttgtg aaaaccacgg tactggataa cacgccgatc cgctggtttt
     2041 accgggcggc cagcctggag tccgcgcagt gggcggcggg gcagaccggg gaaatcaggg
     2101 tggacgtgga gcgccgccgt gccagccggg aggcggggaa cgtggagcat atcagcgggg
     2161 acagcttcat ccagaaggag gcgcggccgc tgtttgacgt gaacaccctg caacacctgc
     2221 cggacgggtt cgcggtcatg accgggctgg gtgtggcccg gatggcgttc agcagtccgc
     2281 tgcgggttga gcgccgggaa atcccgttgc agcgtttccc ggtgctggcg aaaaccgatc
     2341 ccctggcgga gttccggccc ccggccgacg gcggcgggcc gaaagcggat gatgattttg
     2401 cggggattta ctgatggcgc tggagctgta caacgtcagc cacgcgaaac gacaggcgcg
     2461 gaacgcggag aaaacccggc tgaccctgcg ctggttgcgg gaagagctgt gttccacggc
     2521 ggagctggtt gcgcggcggc tggggattgc ggcggtgcag ccggtctacc gcttcctcga
     2581 cagcctggtg gcgaaagggc tgctggtccg gaccaaatat ccggtggacg gccgtcaggt
     2641 cagcgtgtgg gggctgacgc cgcatggcgt ggcgttctcg ttcgacgagg acgaaccgct
     2701 ggcggatgtt attccgtttc agccgtcaag ggtgtcggca gcacatctgc cgcaccggct
     2761 ggccgtgcaa tccctgcgcc ttgatatgga agccagaggg gccaccggct ggcgcattct
     2821 gcaccgtctg agtcttaaag gcatgaaagt gccggacgcc ctggcggagc tggacggtaa
     2881 aacggtggcg tttgaggtgg agcgcaccgt caagagccgt cgccggtatc aggaagtggt
     2941 gtccgggtat ctgttcaacc gcaaagctaa cggcatcgat gaaatctggt acatctgccc
     3001 ggaccgggcg acgcaggtca gggtgcagcg ggcaatcctg tcggtggatg aaatcgtgaa
     3061 cccacagacc ggcgaggccc gcaaaacggc agagctggat cgtgaacggt tgtttgcgtg
     3121 ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat ttattgaaga
     3181 cgcggaggac cggaacggcg agcgctgcat catgccgacc agcgagcatc tggaagatat
     3241 ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc gggcccagca
     3301 tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc
     3361 cacgtacttt ttcactggta aaaatccgca atgggaacgg aacaaaccca caggaatgaa
     3421 gctgtgtgat tacctctatc gcaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt
     3481 ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgacg tggtgagtga
     3541 gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa
     3601 cagccgctat tttaacgtgc cggtacctga actgccggag gacaaacctg cccgccggtg
     3661 gtggccgttc ggcaaaaaat aaatgccagc accggccatg ccggtgctgt tattcctccc
     3721 cgagcttatc ccgcatcacc tgccagagtg tttccgcatc ctcgtgtagc cgctcgcaca
     3781 tgtcggccgg ggtatccata tccagctcat tcagtttatc gagcaatagc agcgactgct
     3841 ccttaataaa tttagccata ttgagggctg ttttttcctg ttttgtcatg attctttcct
     3901 tccgggcatg gtgtgcgcct gtcaggtata ctgtctcaaa aatagcgggg tgagacaatg
     3961 gcgtgggtga cggtgagaca ggcgacggaa ctgacgggta aagcccgaag ttccctgtat
     4021 cgggatatgg cgaaaggccg cgtatcatac cgtacggaag ctgatggcgg ccgggtggtg
     4081 gataccagcg agcttatccg ggtgtacggt gagttaagac aggatgagac tcacgagcgg
     4141 gacgagttga gactgtcggg tgagactgaa aaaatggcct ccgacgcgtt gatcgcggag
     4201 ataaaagcac tcagggagga ggtggccggg ctacggcagg aaatgcagtc catgagactg
     4261 ctggaacaca aaccggagac agcagagacc ccggccggaa caccgcgccg gtggtggccg
     4321 tggggaaaac gctgacgcgc tgtgcttgtc caaccggcga cggccacgca atgaattgcg
     4381 tccccgtcac cggttattag agaacaatat ggggattctg gcgctggccg accacggcca
     4441 gaacccggac ttttctggtc tggtcatcaa catcatagag gacatgctgt cccatcagac
     4501 tgatccggcg aacgtcctcg ccatagcgtg agtccacttc atagagtcgc gggaagtcgg
     4561 ccagtttgtc cgccgcagcg agaacccggt cggtaacgtt ctgcgcatcc cacagggatg
     4621 aattttccgc gatatagtca aacagcgtcg ttacatcctc gatggctccc tgcttccatt
     4681 ccacgtcata cggcatcgcg cagttcctga taacggccat gcaggcgctg gcggagttgt
     4741 tccgtggtca tacccggacg cgtgtcagca cgggcggcgg tcactttggc gcgaacctga
     4801 tcttcatact ctgcctgggc cagttcttcg ttagccagtc gctgcaacag cgcagctttg
     4861 tccgggccac cttccggcag tatgcggggc aggctttcag ccagttcccg aagaaagttg
     4921 gcgttttcgt agtgtgtagt gttgctcatt gcggagcctc ccgggttaat gcggcaatat
     4981 gaaggaagtt tagcaggggg cggtctgaaa gaatatggtc gtgtcagtca ggctgatgaa
     5041 gcagaaagtc ggtaaaaaag ggcttacggc atccatttta cgtcaaaaca tatgccatgc
     5101 agttgcgtgc ataggcatgc attaggataa aatttatcgg gcgcgtttcc ggcagttttc
     5161 cgggggggtt gttgcctgtt ttatcccgta gccgccggaa acgccctgag cccgtctgag
     5221 cggtgcgcgt aatgacgcgt tatggtaaat ccacttactg tctgccttca tagccatcga
     5281 gataaacagc tgaaatggtg tcagccagta gccttgattt tttcggtgtg aggtctttgc
     5341 tgcggtaatc ggtacccgca aattgtgttg tgccttggag tgagtttaaa aattcctgac
     5401 tgtgaaactg ctgaatgcgt tgctgcacat cactccgtga ttttccggtc gctttatcct
     5461 ttgcgcccat atcccagaac tgccggaaaa aaggcaccat cattcgtgtt gtgtcagctt
     5521 tctgggtgga aaaattggga tcaaaaccgt catctgaagc atatacgtaa tgccgggtat
     5581 gctgttccac gctgcccttt ggagcacagg cagtgagagt tgctgtcgct gttatcatgg
     5641 cgataagaca cagtttgctg ccggaacgtc tcatgagcgt ctccttacct ttaattcttt
     5701 tttctcattg agttatatat ccgcaggcta tcttatcatc agtttctgac cgtgttcagt
     5761 gaccgaacaa cccgaaccag accccccgtt tcttttcctg tcccggaggg ggctttctgg
     5821 tatctcatat aatgatttca gtaaaacata ctgaggccgg agaacggcgc cgtttgtgct
     5881 ggtttttgtt gcatctggag gggattgcgc atggtgtgag tggctgtttt tttgatcatt
     5941 ttcacaaaaa cgcaaattta cagacttgac atgaaaaaca aggcagcgta atttttatac
     6001 tgtatataaa accagtggtt atatgtacag tattttattt tagtttattg ttttaaaagt
     6061 caaagaggaa aagtatatga gtggcggaga cggacgaggt ccgggtaatt caggtctggg
     6121 acacaatggt ggtcaggcca gtgggaatgt gaacggtaca tcaggtaaag gtggcccttc
     6181 atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacacctaa
     6241 cggggatatt cataactata acccggggga gtttggtaac ggcgggagta aacccggtgg
     6301 taatggcggt aacagcggca atcatagcgg gagctctggt ggcggacagt cttcggccac
     6361 cgcgatggcc ttcggtctgc ctgccttggc cactccgggg gctgaaggac tggctttatc
     6421 cgtttccggc gatgcgttgt cggccgctgt tgctgatgtg ctggctgccc tgaaaggtcc
     6481 gtttaagttt ggtctgtggg ggattgcgat ttacggagtg ctgccttctg agatagcaaa
     6541 agatgatccg aaaatgatgt caaaaatcgt gacgtcattg ccggcagaca cggtaacgga
     6601 gacgccggtc agctccctgc cgctggacca ggcgacggtc agcgtcacta aacgtgtggc
     6661 ggatattgtg aaggacgagc ggcagcatat tgcggttgtc accggccggc caatgagtgt
     6721 tcctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg
     6781 tctcccgtct ctgcaggtga gcgtacctaa aggtgttccg gccgcgaaag ccccgccaaa
     6841 aggcattatt gctgaaaaag gtgattcacg tccggctggt tttacggccg gtggtaactc
     6901 ccgtgaagcc gttattcgtt tcccgaaaga gagcggacag aaaccggtct atgtgtcggt
     6961 gacggatgtt cttactccgg cacaggtaaa acagcgtctg gaggaagaaa agcgtcgcca
     7021 gcaggcatgg gacgctgctc acccggaaga ggggctgaaa agagagtatg ataaagcgaa
     7081 agctgagctg gatgctgaag ataaaaatat tgcgacctta aacagtcgca ttgcatcgac
     7141 agagaaggcg atccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga
     7201 ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg
     7261 gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa
     7321 agctgcccag acatctttaa acgaaatgaa tgaatcctta tccagggata aggctgcgct
     7381 ttccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa
     7441 attaaatgag gaaaagaaaa aacctcgtaa gggaactaaa gattacggcc atgattattt
     7501 tcctgatccc aagactgaag atattaaggg gttgggagag ttgaaagagg gtaaacctaa
     7561 aacccctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggtgata aaaagcgtaa
     7621 aatttatgaa tgggattccc agcacggtga gcttgaaggg taccgcgcca gtgatggcga
     7681 acacctcggg gcattcgatc caaaaacggg taagcaggtt aaagggccgg atccaaaacg
     7741 aaacattaaa aaatatcttt aagaggtaaa tatggggctt aaattacata ttcattggtt
     7801 tgataagaaa accgaagagt ttaaaggtgg tgaatactca aaagacttcg gtgatgatgg
     7861 ttctgtcatt gaaagtctgg ggatgccttt aaaggataat attaataatg gttggtttga
     7921 tgttgaaaaa tcatgggttt cgatattaca gccacacttt aaaaatgtaa tcgatattag
     7981 taaatttgat tactttgtat cctttgttta ccgggatggt aactggtaag caaaagttat
     8041 caaggatgat ttgaaataca gacttccggt gttcagggat gaataccggg gtttttaaat
     8101 acagggaagg tatatgaaaa aggtaaaggt tgtttttctt tttatattga ttttttcagg
     8161 ctttcttctg gtcgcatgtc aggcaaacta tatccgggat gttcagggtg gaacggtggc
     8221 accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct
     8281 tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg
     8341 ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct
     8401 ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt
     8461 caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct
     8521 gctgccagtg gcgttttgcg tgcttttccg ggttggactc aagtgaacag ttaccggata
     8581 aggcgcagca gtcgggctga acggggggtt cttgcttaca gcccagcttg gagcgaacga
     8641 cctacaccga gccgagatac cagagtgtga gctatgagaa agcgccacac ttcccgcaag
     8701 ggagaaaggc ggaacaggta tccgggaaac ggcagggttg gaacaggaga gcgcaagagg
     8761 gagcgattca tcggaaacgg tgatgatctt taagtcctgt cgggtttcgc cactcctgac
     8821 tgattcatgg ttgagccacc ggctcccaca gatgcaccga aaaagcgtct gtttatgtga
     8881 actcagtcag gagggcggag cctatggaaa aacgccacct gcgtggcctt gccgttttgc
     8941 ctcacatgtt agtcccctgc ttattcacgg aatctgtggg taactttgca tgtgtccgca
     9001 gcgcccgccg cagtctcacg gccggagcgt agcgaccgag tgagcgagga ggcgcaattt
     9061 ccccggcacc tacaagacgg acaccggacg cgctgatgtg gcatattgtc ccctgattaa
     9121 gtctttatat tccctgataa taaattacca ggataggtga atcatatcgg tttttcgctg
     9181 aacattttac gttaacctat ctctgttgct tttttctcct tacggagaaa tacaggtcat
     9241 ccccgcgagg gcgtggctga cctcaggggg atatcccctg cttacgctgg ctgg
//
