LOCUS       NZ_CP098341             9294 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Klebsiella pneumoniae strain 53015_G7 plasmid p53015-9.3, complete
            sequence.
ACCESSION   NZ_CP098341
VERSION     NZ_CP098341.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28680538
            Assembly: GCF_023658145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Chen,L.
  TITLE     Genomic Characterization of Multidrug-Resistant Enterobacterales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2022) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 Ideation Way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023658145.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:25:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,643
            CDSs (total)                      :: 5,519
            Genes (coding)                    :: 5,395
            CDSs (with protein)               :: 5,395
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 46 of 124
            Pseudo Genes (incomplete)         :: 80 of 124
            Pseudo Genes (internal stop)      :: 13 of 124
            Pseudo Genes (multiple problems)  :: 14 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP098341.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="53015_G7"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p53015-9.3"
                     /geo_loc_name="USA"
                     /collection_date="2017/2018"
     gene            complement(join(8241..9294,1..632))
                     /locus_tag="NB667_RS27240"
                     /old_locus_tag="NB667_27245"
     CDS             complement(join(8241..9294,1..632))
                     /locus_tag="NB667_RS27240"
                     /old_locus_tag="NB667_27245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1035..1454
                     /locus_tag="NB667_RS27245"
                     /old_locus_tag="NB667_27250"
     CDS             1035..1454
                     /locus_tag="NB667_RS27245"
                     /old_locus_tag="NB667_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1760..2023
                     /locus_tag="NB667_RS27250"
                     /old_locus_tag="NB667_27255"
     CDS             1760..2023
                     /locus_tag="NB667_RS27250"
                     /old_locus_tag="NB667_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2013..2312
                     /locus_tag="NB667_RS27255"
                     /old_locus_tag="NB667_27260"
     CDS             2013..2312
                     /locus_tag="NB667_RS27255"
                     /old_locus_tag="NB667_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2374..2751)
                     /locus_tag="NB667_RS27260"
                     /old_locus_tag="NB667_27265"
     CDS             complement(2374..2751)
                     /locus_tag="NB667_RS27260"
                     /old_locus_tag="NB667_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            2820..2999
                     /locus_tag="NB667_RS27265"
                     /old_locus_tag="NB667_27270"
     CDS             2820..2999
                     /locus_tag="NB667_RS27265"
                     /old_locus_tag="NB667_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3027..3557)
                     /locus_tag="NB667_RS27270"
                     /old_locus_tag="NB667_27275"
     CDS             complement(3027..3557)
                     /locus_tag="NB667_RS27270"
                     /old_locus_tag="NB667_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3564..4295)
                     /locus_tag="NB667_RS27275"
                     /old_locus_tag="NB667_27280"
     CDS             complement(3564..4295)
                     /locus_tag="NB667_RS27275"
                     /old_locus_tag="NB667_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(4295..6259)
                     /locus_tag="NB667_RS27280"
                     /old_locus_tag="NB667_27285"
     CDS             complement(4295..6259)
                     /locus_tag="NB667_RS27280"
                     /old_locus_tag="NB667_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7740..7889)
                     /locus_tag="NB667_RS27285"
                     /old_locus_tag="NB667_27290"
     CDS             complement(7740..7889)
                     /locus_tag="NB667_RS27285"
                     /old_locus_tag="NB667_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7974..8231)
                     /locus_tag="NB667_RS27290"
                     /old_locus_tag="NB667_27295"
     CDS             complement(7974..8231)
                     /locus_tag="NB667_RS27290"
                     /old_locus_tag="NB667_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7059..7637
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3568..4295)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 cggccggtga caaccgcaat atgctgccgc tcgtccttca caatatccgc cacacgttta
       61 gtgacgctga ccgtcgcctg gtccagcggc agggagctga ccggcgtctc cgttaccgtg
      121 tctgccggca atgacgtcac gatttttgac atcattttcg gatcatcttt tgctatctca
      181 gaaggcagca ctccgtaaat cgcaatcccc cacagaccaa acttaaacgg acctttcagg
      241 gcagccagca catcagcaac agcggccgac aacgcatcgc cggaaacgga taaagccagt
      301 ccttcagccc ccggagtggc caaggcaggc agaccgaagg ccatcgcggt ggccgaagac
      361 tgtccgccac cagagctccc gctatgattg ccgctgttac cgccattacc accgggttta
      421 ctcccgccgt taccaaactc ccccgggtta tagttatgaa tatccccgtt aggtgtatgc
      481 gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga agggccacct
      541 ttacctgatg taccgttcac attcccactg gcctgaccac cattgtgtcc cagacctgaa
      601 ttacccggac ctcgtccgtc tccgccactc atatactttt cctctttgac ttttaaaaca
      661 ataaactaaa ataaaatact gtacatataa ccactggttt tatatacagt ataaaaatta
      721 cgctgccttg tttttcatgt caagtctgta aatttgcgtt tttgtgaaaa tgatcaaaaa
      781 aacagccact cacaccatgc gcaatcccct ccagatgcaa caaaaaccag cacaaacggc
      841 gccgttctcc ggcctcagta tgttttactg aaatcattat atgagatacc agaaagcccc
      901 ctccgggaca ggaaaagaaa cggggggtct ggttcgggtt gttcggtcac tgaacacggt
      961 cagaaactga tgataagata gcctgcggat atataactca atgagaaaaa agaattaaag
     1021 gtaaggagac gctcatgaga cgttccggca gcaaactgtg tcttatcgcc atgataacag
     1081 cgacagcaac tctcactgcc tgtgctccaa agggcagcgt ggaacagcat acccggcatt
     1141 acgtatatgc ttcagatgac ggttttgatc ccaatttttc cacccagaaa gctgacacaa
     1201 cacgaatgat ggtgcctttt ttccggcagt tctgggatat gggcgcaaag gataaagcga
     1261 ccggaaaatc acggagtgat gtgcagcaac gcattcagca gtttcacagt caggaatttt
     1321 taaactcact ccaaggcaca acacaatttg cgggtaccga ttaccgcagc aaagacctca
     1381 caccgaaaaa atcaaggcta ctggctgaca ccatttcagc tgtttatctc gatggctatg
     1441 aaggcagaca gtaagtggat ttaccataac gcgtcattac gcgcaccgct cagacgggct
     1501 cagggcgttt ccggcggcta cgggataaaa caggcaacaa cccccccgga aaactgccgg
     1561 aaacgcgccc gataaatttt atcctaatgc atgcctatgc acgcaactgc atggcatatg
     1621 ttttgacgta aaatggatgc cgtaagccct tttttaccga ctttctgctt catcagcctg
     1681 actgacacga ccatattctt tcagaccgcc ccctgctaaa cttccttcat attgccgcat
     1741 taacccggga ggctccgcaa tgagcaacac tacacactac gaaaacgcca actttcttcg
     1801 ggaactggct gaaagcctgc cccgcatact gccggaaggt ggcccggaca aagctgcgct
     1861 gttgcagcga ctggctaacg aagaactggc ccaggcagag tatgaagatc aggttcgcgc
     1921 caaagtgacc gccgcccgtg ctgacacgcg tccgggtatg accacggaac aactccgcca
     1981 gcgcctgcat ggccgttatc aggaactgcg cgatgccgta tgacgtggaa tggaagcagg
     2041 gagccatcga ggatgtaacg acgctgtttg actatatcgc ggaaaattca tccctgtggg
     2101 atgcgcagaa cgttaccgac cgggttctcg ctgcggcgga caaactggcc gacttcccgc
     2161 gactctatga agtggactca cgctatggcg aggacgttcg ccggatcagt ctgatgggac
     2221 agcatgtcct ctatgatgtt gatgaccaga ccagaaaagt ccgggttctg gccgtggtcg
     2281 gccagcgcca gaatccccat attgttctct aataaccggt gacggggacg caattcattg
     2341 cgtggccgtc gccggttgga caagcacagc gcgtcagcgt tttccccacg gccaccaccg
     2401 gcgcggtgtt ccggccgggg tctctgctgt ctccggtttg tgttccagca gtctcatgga
     2461 ctgcatttcc tgccgtagcc cggccacctc ctccctgagt gcttttatct ccgcgatcaa
     2521 cgcgtcggag gccatttttt cagtctcacc cgacagtctc aactcgtccc gctcgtgagt
     2581 ctcatcctgt cttaactcac cgtacacccg gataagctcg ctggtatcca ccacccggcc
     2641 gccatcagct tccgtacggt atgatacgcg gcctttcgcc atatcccgat acagggaact
     2701 tcgggcttta cccgtcagtt ccgtcgcctg tctcaccgtc acccacgcca ttgtctcacc
     2761 ccgctatttt tgagacagta tacctgacag gcgcacacca tgcccggaag gaaagaatca
     2821 tgacaaaaca ggaaaaaaca gccctcaata tggctaaatt tattaaggag cagtcgctgc
     2881 tattgctcga taaactgaat gagctggata tggatacccc ggccgacatg tgcgagcggc
     2941 tacacgagga tgcggaaaca ctctggcagg tgatgcggga taagctcggg gaggaataac
     3001 agcaccggca tggccggtgc tggcatttat tttttgccga acggccacca ccggcgggca
     3061 ggtttgtcct ccggcagttc aggtaccggc acgttaaaat agcggctgtt atattcccgc
     3121 atcagctccg ctttcagctt ctcgttcacc gcgctttccc cgtgccgctc actcaccacg
     3181 tcaatcatcg cgttaatctg ctccggtgag cacttcccgg catcaaccac ctgcacaccg
     3241 ttctcgatgg cgaaccgctc caccttgcga tagaggtaat cacacagctt cattcctgtg
     3301 ggtttgttcc gttcccattg cggattttta ccagtgaaaa agtacgtggc tttttcgtcg
     3361 ctgttgagcc gctcgtcata catcccgatg gatttcatca tcgccagatg ctgggcccgg
     3421 tcgacaaaac agaaaacata cgccccggtc tgccaggcga gcaccaccat atcttccaga
     3481 tgctcgctgg tcggcatgat gcagcgctcg ccgttccggt cctccgcgtc ttcaataaat
     3541 accatgtaca gcgccataat ccctcactcc gttgtcataa atttaaagca cgcaaacaac
     3601 cgttcacgat ccagctctgc cgttttgcgg gcctcgccgg tctgtgggtt cacgatttca
     3661 tccaccgaca ggattgcccg ctgcaccctg acctgcgtcg cccggtccgg gcagatgtac
     3721 cagatttcat cgatgccgtt agctttgcgg ttgaacagat acccggacac cacttcctga
     3781 taccggcgac ggctcttgac ggtgcgctcc acctcaaacg ccaccgtttt accgtccagc
     3841 tccgccaggg cgtccggcac tttcatgcct ttaagactca gacggtgcag aatgcgccag
     3901 ccggtggccc ctctggcttc catatcaagg cgcagggatt gcacggccag ccggtgcggc
     3961 agatgtgctg ccgacaccct tgacggctga aacggaataa catccgccag cggttcgtcc
     4021 tcgtcgaacg agaacgccac gccatgcggc gtcagccccc acacgctgac ctgacggccg
     4081 tccaccggat atttggtccg gaccagcagc cctttcgcca ccaggctgtc gaggaagcgg
     4141 tagaccggct gcaccgccgc aatccccagc cgccgcgcaa ccagctccgc cgtggaacac
     4201 agctcttccc gcaaccagcg cagggtcagc cgggttttct ccgcgttccg cgcctgtcgt
     4261 ttcgcgtggc tgacgttgta cagctccagc gccatcagta aatccccgca aaatcatcat
     4321 ccgctttcgg cccgccgccg tcggccgggg gccggaactc cgccagggga tcggttttcg
     4381 ccagcaccgg gaaacgctgc aacgggattt cccggcgctc aacccgcagc ggactgctga
     4441 acgccatccg ggccacaccc agcccggtca tgaccgcgaa cccgtccggc aggtgttgca
     4501 gggtgttcac gtcaaacagc ggccgcgcct ccttctggat gaagctgtcc ccgctgatat
     4561 gctccacgtt ccccgcctcc cggctggcac ggcggcgctc cacgtccacc ctgatttccc
     4621 cggtctgccc cgccgcccac tgcgcggact ccaggctggc cgcccggtaa aaccagcgga
     4681 tcggcgtgtt atccagtacc gtggttttca caaattcagc gggcagatcc tgcccgcact
     4741 gcccgaaatc gcccagcgac tggtgcgcga gcaacagatt gcagtcccgg ctgcgcagcg
     4801 tccccagtgc gttcagtaca tacttcgaca gcagatattt aatttcatcg agcatcacgc
     4861 tgacgtgtgg ccactcccgg aactcgtccc gcgcaatgat gatttgcgcg cagcgggcaa
     4921 acagcatttt ttgcacacgg atcaccgcct cgtcgtccat cgagccgata acgtacaggc
     4981 agccgccgcc gttgataatc cccgccacat cgatgccact gtccgtctgc aggacgctca
     5041 ggtcagcgac gttttccagc ctagtgatca accccttcac gtcctttttc agggcctccg
     5101 gcagcgcgta cgccgccgcc agcacctgct gaatgtttgc cccctgcgga aactgctccg
     5161 caatcagctt cgccgctttc tgctcgctga tgcggtagtg gtccgccacg tcgccggtct
     5221 cgcccaggtt aagcccggca atcagcaggt ttttcagcgc gtactggtcg agatcgcgga
     5281 acagattcag ctgcgccggt ttccccggcc gcagatccag cagggtgaag tccggacagt
     5341 gcgcccggaa gacgtgcggc gcccatgcgt cccctttggg gtcgaagtac accaccgcgt
     5401 ccccgaaatc gcgtacgcag cgaatgagcg cgttggtggc catcacgctc ttgccgctcc
     5461 cgggcaggcc gaggatctga atgttggtct tgcgccaggt atcacgcgga accagcaccg
     5521 gcttgccctg ctcatcctta cccagaaaca ccgcctgctc ctgccgggcc tgctccgagg
     5581 aaaaatcggc cagcgcatta accggtaccg gctccccgaa atgcaggtgg cgcacgtcgg
     5641 tcatctctcc tgtccgctgg ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg
     5701 cctcgtcagc agccgcaaaa tgaccgtcaa gcagcaggaa cgccagcagc cgcgccaaca
     5761 caatcccgac agccagcccg cccgcgcacc aggcaatccc gaacaggtta gccggctgca
     5821 gcaggtaata atctgcgttt gcgtagaggt ccggacgcag gacggtttct ccccagctct
     5881 cgataaacac ggcgacaacc agccccccca gaagggccag accggccgcg ccggtcagcc
     5941 agtacgcgta gttccagaca tgtcgacgtg ccctgaccat ccgcccgggc gaatccagcg
     6001 aggccagcac cgaaaatggc gctggccgtt tattatcgcg cttatagcga tcggcatact
     6061 cccgtcgctc cttatgactc agccccacaa aacgcatccg tccggcccaa agttcagcca
     6121 gaccgaaaaa caggccgata actaggaaag ccccggccag accgtaatac tcctggtaca
     6181 gccagctgac ccacggataa atctgccggg ccagctccgg aatgtgccac ttccaggcaa
     6241 gcgaatccgt attaaacatg ttctctctcc ttcagacgat ggtttgccgg cgtgatgtgc
     6301 agcccccgct gcacaagccg cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt
     6361 ggtgattgcg ttcagctttc accacggagg ccagcctctt tccgccccat gcggaaaagg
     6421 cgcagccccg gaggaacaac gagcacgccc cagcgtgtga gaggaaaagg cggcacccca
     6481 gtgcggtctt ttccggtgtt ccggcccccg tcagagttcg ccatagcgag cggctgacca
     6541 cccgcaaaat caggagcagc tttgctgctt cgccaggtgc gtccagcacc gtggattttt
     6601 gtgggtggtc aggcgtgagc ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc
     6661 agccggacgg agccagaaag agggggagag accgtgcgtc ggtggcgtcc agccagcgta
     6721 agcaggggat atccccctga ggtcagccac gccctcgcgg ggatgacctg tatttctccg
     6781 taaggagaaa aaagcaacag agataggtta acgtaaaatg ttcagcgaaa aaccgatatg
     6841 attcacctat cctggtaatt tattatcagg gaatataaag acttaatcag gggacaatat
     6901 gccacatcag cgcgtccggt gtccgtcttg taggtgccgg ggaaattgcg cctcctcgct
     6961 cactcggtcg ctacgctccg gccgtgagac tgcggcgggc gctgcggaca catgcaaagt
     7021 tacccacaga ttccgtgaat aagcagggga ctaacatgtg aggcaaaacg gcaaggccac
     7081 gcaggtggcg tttttccata ggctccgccc tcctgactga gttcacataa acagacgctt
     7141 tttcggtgca tctgtgggag ccggtggctc aaccatgaat cagtcaggag tggcgaaacc
     7201 cgacaggact taaagatcat caccgtttcc gatgaatcgc tccctcttgc gctctcctgt
     7261 tccaaccctg ccgtttcccg gatacctgtt ccgcctttct cccttgcggg aagtgtggcg
     7321 ctttctcata gctcacactc tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg
     7381 gctgtaagca agaacccccc gttcagcccg actgctgcgc cttatccggt aactgttcac
     7441 ttgagtccaa cccggaaaag cacgcaaaac gccactggca gcagccattg gtaactggga
     7501 gttcgcagag gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc
     7561 ggctacactg gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta
     7621 agcagttccc caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt
     7681 tacagggcaa aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7741 tactgaaccg cgatccccgt cagttcagag gaggacgatg gtgccaccgt tccaccctga
     7801 acatcccgga tatagtttgc ctgacatgcg accagaagaa agcctgaaaa aatcaatata
     7861 aaaagaaaaa caacctttac ctttttcata taccttccct gtatttaaaa accccggtat
     7921 tcatccctga acaccggaag tctgtatttc aaatcatcct tgataacttt tgcttaccag
     7981 ttaccatccc ggtaaacaaa ggatacaaag taatcaaatt tactaatatc gattacattt
     8041 ttaaagtgtg gctgtaatat cgaaacccat gatttttcaa catcaaacca accattatta
     8101 atattatcct ttaaaggcat ccccagactt tcaatgacag aaccatcatc accgaagtct
     8161 tttgagtatt caccaccttt aaactcttcg gttttcttat caaaccaatg aatatgtaat
     8221 ttaagcccca tatttacctc ttaaagatat tttttaatgt ttcgttttgg atccggccct
     8281 ttaacctgct tacccgtttt tggatcgaat gccccgaggt gttcgccatc actggcgcgg
     8341 tacccttcaa gctcaccgtg ctgggaatcc cattcataaa ttttacgctt tttatcacca
     8401 taccatcggg cccgcttacc accaccacct tgtttagggg ttttaggttt accctctttc
     8461 aactctccca accccttaat atcttcagtc ttgggatcag gaaaataatc atggccgtaa
     8521 tctttagttc ccttacgagg ttttttcttt tcctcattta atttattttc tgcatccttc
     8581 gcttttttct ccttttgttt ccggctctcc atcgccccgg aaagcgcagc cttatccctg
     8641 gataaggatt cattcatttc gtttaaagat gtctgggcag cttttaattt ctcattctga
     8701 ttctgaatat ccttatcaag atagcgaacc tgatcctgcc acccggagcc atattcatga
     8761 ggcgggttat aagtcacaaa atcatcttta tttgcctctg cctcttttac ctttttatcg
     8821 gcttcctgaa cagcagccct tgcaccgggg atcgccttct ctgtcgatgc aatgcgactg
     8881 tttaaggtcg caatattttt atcttcagca tccagctcag ctttcgcttt atcatactct
     8941 cttttcagcc cctcttccgg gtgagcagcg tcccatgcct gctggcgacg cttttcttcc
     9001 tccagacgct gttttacctg tgccggagta agaacatccg tcaccgacac atagaccggt
     9061 ttctgtccgc tctctttcgg gaaacgaata acggcttcac gggagttacc accggccgta
     9121 aaaccagccg gacgtgaatc acctttttca gcaataatgc cttttggcgg ggctttcgcg
     9181 gccggaacac ctttaggtac gctcacctgc agagacggga gacccggaat cgacacactg
     9241 aatacccccg gacgttttgt cggtttcgca tccaccacag gaacactcat tggc
//
